{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:06:45Z","timestamp":1774314405405,"version":"3.50.1"},"reference-count":71,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,4,5]],"date-time":"2018-04-05T00:00:00Z","timestamp":1522886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011084","name":"Sigma Xi","doi-asserted-by":"publisher","award":["201503151199414"],"award-info":[{"award-number":["201503151199414"]}],"id":[{"id":"10.13039\/100011084","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011066","name":"Smith College","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology: Parasites and Wildlife"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1016\/j.ijppaw.2018.04.001","type":"journal-article","created":{"date-parts":[[2018,4,6]],"date-time":"2018-04-06T19:08:37Z","timestamp":1523041717000},"page":"147-154","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)"],"prefix":"10.1016","volume":"7","author":[{"given":"Caroline D.","family":"Keroack","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6238-1158","authenticated-orcid":false,"given":"Kalani M.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.K.","family":"Fessler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaela E.","family":"DeAngelis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0946-9254","authenticated-orcid":false,"given":"Eirini","family":"Tsekitsidou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jillian M.","family":"Tozloski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven A.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijppaw.2018.04.001_bib1","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.pt.2015.04.006","article-title":"Expanding the MDx toolbox for filarial diagnosis and surveillance","volume":"31","author":"Alhassan","year":"2015","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib65","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S1055-7903(03)00208-2","article-title":"Ribosomal ITS sequences and plant phylogenetic inference","volume":"29","author":"\u00c1lvarez","year":"2003","journal-title":"Mol. Phylogenet. Evol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib2","doi-asserted-by":"crossref","first-page":"S16","DOI":"10.1038\/nrmicro1523z","article-title":"Evaluation of diagnostic tests for infectious diseases: general principles","volume":"8","author":"Banoo","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib3","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1093\/bioinformatics\/btq281","article-title":"Manipulation of FASTQ data with Galaxy","volume":"26","author":"Blankenberg","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib4","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1098\/rstb.2003.1447","article-title":"The promise of a DNA taxonomy","volume":"359","author":"Blaxter","year":"2004","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib66","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0020-7519(01)00357-5","article-title":"Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer","volume":"32","author":"Blouin","year":"2002","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib5","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1755-0998.2009.02629.x","article-title":"The front-end logistics of DNA barcoding: challenges and prospects","volume":"9","author":"Borisenko","year":"2009","journal-title":"Mol. Ecol. Resour"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib6","doi-asserted-by":"crossref","first-page":"S139","DOI":"10.1093\/cid\/cit578","article-title":"Better tests, better care: improved diagnostics for infectious diseases","volume":"57","author":"Caliendo","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib7","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1017\/S0031182000007149","article-title":"A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts","volume":"122","author":"Casiraghi","year":"2001","journal-title":"Parasitology"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib8","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.ijpara.2003.10.004","article-title":"Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution","volume":"34","author":"Casiraghi","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib9","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1093\/bib\/bbq003","article-title":"DNA barcoding: a six-question tour to improve users' awareness about the method","volume":"11","author":"Casiraghi","year":"2010","journal-title":"Briefings Bioinf."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib10","first-page":"649","article-title":"The helminth fauna from the common seal (Phoca vitulina vitulina, linn\u00e9, 1758) of the wadden sea in lower saxony*","volume":"38","author":"Claussen","year":"1991","journal-title":"Zentralblatt F\u00fcr Veterin\u00e4rmedizin Reihe B J. Vet. Med. Ser. B"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib67","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/S0168-9525(03)00118-5","article-title":"ITS2 is a double-edged tool for eukaryote evolutionary comparisons","volume":"19","author":"Coleman","year":"2003","journal-title":"Trends Genet."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib11","series-title":"Parasites of Marine Mammals","author":"Dailey","year":"2005"},{"issue":"288","key":"10.1016\/j.ijppaw.2018.04.001_bib12","article-title":"Dirofilariosis in the Americas: a more virulent Dirofilaria immitis?","volume":"6","author":"Dantas-Torres","year":"2013","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib13","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1469-0691.2005.01332.x","article-title":"Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis","volume":"12","author":"Edvinsson","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib14","series-title":"Reported Incidences of Parasitic Infections in Marine Mammals from 1892 to 1978","author":"Felix","year":"2013"},{"issue":"1","key":"10.1016\/j.ijppaw.2018.04.001_bib15","doi-asserted-by":"crossref","DOI":"10.1186\/1742-9994-6-1","article-title":"Integrated taxonomy: traditional approach and DNA barcoding for the identification of filarioid worms and related parasites (Nematoda)","volume":"6","author":"Ferri","year":"2009","journal-title":"Front. Zool."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib16","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1046\/j.1365-294X.2002.01485.x","article-title":"Molecular barcodes for soil nematode identification","volume":"11","author":"Floyd","year":"2002","journal-title":"Mol. Ecol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib17","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1645\/GE-2178.1","article-title":"Morphological redescription of Dirofilaria immitis","volume":"96","author":"Furtado","year":"2010","journal-title":"J. Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib18","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1006\/jipa.1997.4737","article-title":"The ribosomal RNA gene region of nosema apis (microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size","volume":"71","author":"Gatehouse","year":"1998","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib19","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1139\/z81-197","article-title":"The seal louse, Echinophthirius horridus: an intermediate host of the seal heartworm, Dipetalonema spirocauda","volume":"59","author":"Geraci","year":"1981","journal-title":"Can. J. Zool."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib20","doi-asserted-by":"crossref","first-page":"545","DOI":"10.7589\/0090-3558-30.4.545","article-title":"Factors affecting Dirofilaria immitis prevalence in red foxes in northeastern Spain","volume":"30","author":"Gortazar","year":"1994","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib68","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1093\/oxfordjournals.molbev.a026308","article-title":"Intragenomic variation within ITS1 and ITS2 of freshwater crayfishes (Decapoda: Cambaridae): implications for phylogenetic and microsatellite studies","volume":"17","author":"Harris","year":"2000","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib21","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1126\/science.1063699","article-title":"Climate warming and disease risks for terrestrial and marine biota","volume":"296","author":"Harvell","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib22","doi-asserted-by":"crossref","first-page":"753","DOI":"10.4269\/ajtmh.2011.11-0009","article-title":"Polymerase chain reaction pool screening used to compare prevalence of infective black flies in two onchocerciasis foci in northern Sudan","volume":"84","author":"Higazi","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib69","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3354\/dao02738","article-title":"Phylogenetics of Bonamia parasites based on small subunit and internal transcribed spacer region ribosomal DNA sequence data","volume":"110","author":"Hill","year":"2014","journal-title":"Dis. Aquat. Organ"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib23","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/molbev\/msl044","article-title":"Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades","volume":"23","author":"Holterman","year":"2006","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib24","doi-asserted-by":"crossref","first-page":"229","DOI":"10.7589\/0090-3558-16.2.229","article-title":"Dirofilaria immitis in red foxes in Illinois","volume":"16","author":"Hubert","year":"1980","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib25","doi-asserted-by":"crossref","DOI":"10.1645\/15-872","article-title":"Absence of the filarial endosymbiont Wolbachia in seal heartworm (Acanthocheilonema spirocauda) but evidence of ancient lateral gene transfer","volume":"102","author":"Keroack","year":"2016","journal-title":"J. Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib26","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s10152-007-0072-9","article-title":"Parasites in harbour seals (Phoca vitulina) from the German wadden sea between two phocine distemper virus epidemics","volume":"61","author":"Lehnert","year":"2007","journal-title":"Helgol. Mar. Res."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib27","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.seares.2015.06.014","article-title":"Heartworm (Acanthocheilonema spirocauda) and seal louse (Echinophthirius horridus) infections in harbour seals (Phoca vitulina) from the North and Baltic Seas","volume":"113","author":"Lehnert","year":"2015","journal-title":"J. Sea Res."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib28","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/j.ijpara.2009.12.008","article-title":"Transmission of lungworms of harbour porpoises and harbour seals: molecular tools determine potential vertebrate intermediate hosts","volume":"40","author":"Lehnert","year":"2010","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib29","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3354\/dao01823","article-title":"Phocid seals, seal lice and heartworms: a terrestrial host-parasite system conveyed to the marine environment","volume":"77","author":"Leidenberger","year":"2007","journal-title":"Dis. Aquat. Org."},{"issue":"44","key":"10.1016\/j.ijppaw.2018.04.001_bib30","article-title":"Chabertia erschowi (Nematoda) is a distinct species based on nuclear ribosomal DNA sequences and mitochondrial DNA sequences","volume":"7","author":"Liu","year":"2014","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib31","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1080\/00364827.1992.10413483","article-title":"Dipetalonema spirocauda (leidy) (nematoda) and corynosoma strumosum (rudolphi) (acanthocephala) infection in harbour seal from the kattegat-skagerrak and the baltic","author":"Lunneryd","year":"1992","journal-title":"Sarsia"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib32","series-title":"Phobos 3.3.11","author":"Mayer","year":"2006"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib33","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1186\/1471-2164-11-277","article-title":"Genome-wide analysis of tandem repeats in Daphnia pulex-a comparative approach","volume":"11","author":"Mayer","year":"2010","journal-title":"BMC Genom."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib34","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1073\/pnas.83.3.797","article-title":"Cloning and comparison of repeated DNA sequences from the human filarial parasite Brugia malayi and the animal parasite Brugia pahangi","volume":"83","author":"McReynolds","year":"1986","journal-title":"Proc. Natl. Acad. Sci. Unit. States Am."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib35","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1139\/f96-342","article-title":"Heartworm, Acanthocheilonema spirocauda (Leidy, 1858), infections in Canadian phocid seals","volume":"54","author":"Measures","year":"1997","journal-title":"Can. J. Fish. Aquat. Sci."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib36","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.4269\/ajtmh.12-0726","article-title":"A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations","volume":"88","author":"Mejia","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib37","doi-asserted-by":"crossref","first-page":"e354","DOI":"10.1371\/journal.pbio.0020354","article-title":"DNA barcoding: promise and pitfalls","volume":"2","author":"Moritz","year":"2004","journal-title":"PLoS Biol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib70","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.protis.2009.07.003","article-title":"Barcoding amoebae: comparison of SSU, ITS and COI genes as tools for molecular identification of naked lobose amoebae","volume":"161","author":"Nassonova","year":"2010","journal-title":"Protist"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib38","doi-asserted-by":"crossref","first-page":"e111","DOI":"10.1093\/nar\/gkx257","article-title":"TAREAN: a computational tool for identification and characterization of satellite DNA from unassembled short reads","volume":"45","author":"Nov\u00e1k","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2105-11-378","article-title":"Graph-based clustering and characterization of repetitive sequences in next-generation sequencing data","volume":"11","author":"Nov\u00e1k","year":"2010","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib40","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1093\/bioinformatics\/btt054","article-title":"RepeatExplorer: a Galaxy-based web server for genome-wide characterization of eukaryotic repetitive elements from next-generation sequence reads","volume":"29","author":"Nov\u00e1k","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib41","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1128\/MMBR.00033-09","article-title":"Mapping the tree of life: progress and prospects","volume":"73","author":"Pace","year":"2009","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib42","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/j.1755-0998.2009.02631.x","article-title":"DNA barcoding and the mediocrity of morphology","volume":"9","author":"Packer","year":"2009","journal-title":"Mol. Ecol. Resour"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib43","doi-asserted-by":"crossref","first-page":"570","DOI":"10.7589\/0090-3558-46.2.570","article-title":"First report of Acanthocheilonema spirocauda in the mediterranean monk seal (monachus monachus)","volume":"46","author":"Papadopoulos","year":"2010","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib44","series-title":"Improved Molecular Diagnostics for the Detection of Soil-transmitted Helminthes: a Multi-parallel qPCR Based Assay","author":"Papaiakovou","year":"2014"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib45","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004578","article-title":"Improved PCR-based detection of soil transmitted helminth infections using a next-generation sequencing approach to assay design","volume":"10","author":"Pilotte","year":"2016","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib46","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/j.pt.2017.06.008","article-title":"The current status of molecular xenomonitoring for lymphatic Filariasis and onchocerciasis","volume":"33","author":"Pilotte","year":"2017","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib47","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004641","article-title":"A novel xenomonitoring technique using mosquito excreta\/feces for the detection of filarial parasites and malaria","volume":"10","author":"Pilotte","year":"2016","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib48","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1146\/annurev.phyto.42.040803.140348","article-title":"NEMATODE molecular diagnostics: from bands to barcodes","volume":"42","author":"Powers","year":"2004","journal-title":"Annu. Rev. Phytopathol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib49","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004722","article-title":"Programmatic use of molecular xenomonitoring at the level of evaluation units to assess persistence of lymphatic Filariasis in Sri Lanka","volume":"10","author":"Rao","year":"2016","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib50","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1177\/1087057114548065","article-title":"Diagnosis of parasitic infections What's going on?","volume":"20","author":"Ricciardi","year":"2015","journal-title":"J. Biomol. Screen"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib51","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.vetpar.2005.10.013","article-title":"Discrimination between six species of canine microfilariae by a single polymerase chain reaction","volume":"135","author":"Rishniw","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib52","doi-asserted-by":"crossref","DOI":"10.1186\/s13071-015-0783-8","article-title":"Detection of Dirofilaria immitis and other arthropod-borne filarioids by an HRM real-time qPCR, blood-concentrating techniques and a serological assay in dogs from Costa Rica","volume":"8","author":"Rojas","year":"2015","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib53","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1093\/bioinformatics\/btg326","article-title":"MREPATT: detection and analysis of exact consecutive repeats in genomic sequences","volume":"19","author":"Roset","year":"2003","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib54","doi-asserted-by":"crossref","first-page":"e62221","DOI":"10.1371\/journal.pone.0062221","article-title":"A satellite explosion in the genome of holocentric nematodes","volume":"8","author":"Subirana","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib55","doi-asserted-by":"crossref","first-page":"971","DOI":"10.2307\/3275035","article-title":"Biology of filarioid nematode Dipetalonema spirocauda (Leidy, 1858) from the heart of captive harbour seals and sea lions, together with pathology of the hosts","volume":"47","author":"Taylor","year":"1961","journal-title":"J. Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib56","article-title":"Rapid detection of Brugia malayi in mosquito vectors using a real-time fluorescence resonance energy trans- fer PCR and melting curve analysis","volume":"3","author":"Thanchomnang","year":"2008","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib57","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1126\/science.282.5396.2012","article-title":"Genome sequence of the nematode C. elegans: a platform for investigating Biology","volume":"282","author":"The C. elegans Sequencing Consortium","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib58","article-title":"Repetitive DNA and next-generation sequencing: computational challenges and solutions","author":"Treangen","year":"2011","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib59","first-page":"22","article-title":"Mededelingen betreffende parasitologisch onderzoek bij de gewone zeehond, Phoca vitulina","volume":"5","author":"van den Broek","year":"1963","journal-title":"Lutra"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib60","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1006\/expr.2000.4563","article-title":"Amplification of a specific repetitive DNA sequence for trypanosoma rangeli identification and its potential application in epidemiological investigations","volume":"96","author":"Vargas","year":"2000","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib61","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1002\/bies.20529","article-title":"DNA barcoding in animal species: progress, potential and pitfalls","volume":"29","author":"Waugh","year":"2007","journal-title":"Bioessays"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib62","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1080\/10635150500354878","article-title":"The perils of DNA barcoding and the need for integrative taxonomy","volume":"54","author":"Will","year":"2005","journal-title":"Syst. Biol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib63","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1096-0031.2003.00008.x","article-title":"Myth of the molecule: DNA barcodes for species cannot replace morphology for identification and classification","volume":"20","author":"Will","year":"2004","journal-title":"Cladistics"},{"key":"10.1016\/j.ijppaw.2018.04.001_bib71","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/S1055-7903(03)00178-7","article-title":"The advantages of the ITS2 region of the nuclear rDNA cistron for analysis of phylogenetic relationships of insects: a Drosophila example","volume":"30","author":"Young","year":"2004","journal-title":"Mol. Phylogenet. Evol."},{"key":"10.1016\/j.ijppaw.2018.04.001_bib64","doi-asserted-by":"crossref","first-page":"357","DOI":"10.4269\/ajtmh.1996.54.357","article-title":"A polymerase chain reaction assay for detection of the parasite Wuchereria bancrofti in human blood samples","volume":"54","author":"Zhong","year":"1996","journal-title":"Am. J. Trop. Med. Hyg."}],"container-title":["International Journal for Parasitology: Parasites and Wildlife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213224418300129?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213224418300129?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T05:45:23Z","timestamp":1761975923000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2213224418300129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":71,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["S2213224418300129"],"URL":"https:\/\/doi.org\/10.1016\/j.ijppaw.2018.04.001","relation":{},"ISSN":["2213-2244"],"issn-type":[{"value":"2213-2244","type":"print"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology: Parasites and Wildlife","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijppaw.2018.04.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.","name":"copyright","label":"Copyright"}]}}