{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:00:25Z","timestamp":1780549225202,"version":"3.54.1"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,12,7]],"date-time":"2024-12-07T00:00:00Z","timestamp":1733529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology: Parasites and Wildlife"],"published-print":{"date-parts":[[2025,4]]},"DOI":"10.1016\/j.ijppaw.2024.101031","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T03:13:45Z","timestamp":1733886825000},"page":"101031","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Molecular-based laboratory testing confer accuracy over microscopical testing for tick identification"],"prefix":"10.1016","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7078-0411","authenticated-orcid":false,"given":"Isaac","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0205-9482","authenticated-orcid":false,"given":"Min-Kuang","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Kon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navdeep","family":"Chahil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Hogan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Morshed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"7","key":"10.1016\/j.ijppaw.2024.101031_bib1","first-page":"693","article-title":"The increasing risk of Lyme disease in Canada","volume":"56","author":"Bouchard","year":"2015","journal-title":"Can. Vet. J."},{"issue":"3","key":"10.1016\/j.ijppaw.2024.101031_bib2","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1093\/jme\/tjad042","volume":"60","author":"Burtis","year":"2023","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.ijppaw.2024.101031_bib3","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s13071-020-3893-x","article-title":"Comparative vector competence of North American Lyme disease vectors","volume":"13","author":"Couper","year":"2020","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.ijppaw.2024.101031_bib22","series-title":"Bulletin of the California Insect Survey. 25","author":"Furman","year":"1984"},{"issue":"12","key":"10.1016\/j.ijppaw.2024.101031_bib5","doi-asserted-by":"crossref","first-page":"2412","DOI":"10.3390\/microorganisms9122412","article-title":"The role of ticks in the emergence of Borrelia burdorferi as a zoonotic pathogen and its vector control: a global systemic review","volume":"9","author":"Hussain","year":"2021","journal-title":"Microorganisms"},{"issue":"8","key":"10.1016\/j.ijppaw.2024.101031_bib6","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.3390\/genes14081592","article-title":"Morphological and molecular identification of hard ticks in Hainan Island, China","volume":"14","author":"Intirach","year":"2023","journal-title":"Genes"},{"issue":"62","key":"10.1016\/j.ijppaw.2024.101031_bib7","first-page":"3923","article-title":"Agarose gel electrophoresis for the separation of DNA fragments","author":"Lee","year":"2012","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.ijppaw.2024.101031_bib8","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/1756-3305-7-93","article-title":"Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida)","volume":"7","author":"Lv","year":"2014","journal-title":"Parasites Vectors"},{"issue":"1","key":"10.1016\/j.ijppaw.2024.101031_bib9","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1093\/jmedent\/47.1.99","article-title":"Ecological niche modeling of lyme disease in British Columbia, Canada","volume":"47","author":"Mak","year":"2010","journal-title":"J. Med. Entomol."},{"issue":"11","key":"10.1016\/j.ijppaw.2024.101031_bib10","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1089\/vbz.2015.1854","article-title":"Surveillance for Borrelia burgdorferi in Ixodes ticks and small rodents in British Columbia","volume":"15","author":"Morshed","year":"2015","journal-title":"Vector Borne Zoonotic Dis."},{"issue":"2","key":"10.1016\/j.ijppaw.2024.101031_bib11","doi-asserted-by":"crossref","first-page":"348","DOI":"10.3390\/pathogens12020348","article-title":"Ticks and rickettsiae associated with wild animals sold in bush meat markets in Cameroon","volume":"12","author":"Paguem","year":"2023","journal-title":"J. Pathog."},{"issue":"4","key":"10.1016\/j.ijppaw.2024.101031_bib12","doi-asserted-by":"crossref","first-page":"e42","DOI":"10.1097\/OLQ.0000000000000935","article-title":"Increased discrimination of Treponema pallidum strains by subtyping with a 4-component system incorporating a mononucleotide tandem repeat in rpsA","volume":"46","author":"Pillay","year":"2019","journal-title":"Sex. Transm. Dis."},{"key":"10.1016\/j.ijppaw.2024.101031_bib13","article-title":"Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiences","volume":"11","author":"Pradhan","year":"2023","journal-title":"Front. Public Health"},{"issue":"3","key":"10.1016\/j.ijppaw.2024.101031_bib14","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1471-8286.2007.01678.x","article-title":"BOLD: the Barcode of Life data system (www.barcodinglife.org)","volume":"7","author":"Ratnasingham","year":"2006","journal-title":"Mol. Ecol."},{"issue":"3\u20134","key":"10.1016\/j.ijppaw.2024.101031_bib15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.14745\/ccdr.v50i34a02","article-title":"Innovations in public health surveillance: an overview of novel use of data and analytic methods","volume":"50","author":"Rilkoff","year":"2024","journal-title":"Can. Comm. Dis. Rep."},{"issue":"1","key":"10.1016\/j.ijppaw.2024.101031_bib16","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1111\/j.1948-7134.2010.00068.x","article-title":"Detection of Lyme disease spirochete, Borrelia burgdorferi sensu lato, including three novel genotypes in ticks (Acari: Ixodidae) collected from songbirds (Passeriformes)across Canada","volume":"35","author":"Scott","year":"2010","journal-title":"J. Vector Ecol."},{"issue":"5","key":"10.1016\/j.ijppaw.2024.101031_bib17","doi-asserted-by":"crossref","first-page":"935","DOI":"10.37871\/jbres1754","article-title":"Primary detection of the establishment of blacklegged ticks, Ixodes scapularis, in British Columbia, Canada","volume":"4","author":"Scott","year":"2023","journal-title":"J. Biomed. Res. Environ. Sci."},{"issue":"5","key":"10.1016\/j.ijppaw.2024.101031_bib18","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1093\/jme\/tjx111","article-title":"The first evidence of nanism in Ixodes (Ixodes) scapularis (Acari: Ixodidae), found parasitizing a human host","volume":"54","author":"Soghigian","year":"2017","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.ijppaw.2024.101031_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.prp.2021.153443","article-title":"Recent advances and challenges of RT-PCR tests for diagnosis of COVID-19","volume":"221","author":"Teymouri","year":"2021","journal-title":"Pathol. Res. Pract."},{"issue":"21","key":"10.1016\/j.ijppaw.2024.101031_bib20","doi-asserted-by":"crossref","first-page":"10221","DOI":"10.1073\/pnas.90.21.10221","article-title":"Investigation of the validity of species status of Ixodes dammini (Acari: Ixodidae) using rDNA","volume":"90","author":"Wesson","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"4","key":"10.1016\/j.ijppaw.2024.101031_bib21","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1093\/jme\/tjac074","article-title":"Development of Taqman real-time PCR for the identification of Haemaphsalis longicornis (Acari: Ixodidae)","volume":"59","author":"Xu","year":"2022","journal-title":"J. Med. Entomol."}],"container-title":["International Journal for Parasitology: Parasites and Wildlife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213224424001275?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213224424001275?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T13:18:05Z","timestamp":1762607885000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2213224424001275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":21,"alternative-id":["S2213224424001275"],"URL":"https:\/\/doi.org\/10.1016\/j.ijppaw.2024.101031","relation":{},"ISSN":["2213-2244"],"issn-type":[{"value":"2213-2244","type":"print"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular-based laboratory testing confer accuracy over microscopical testing for tick identification","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology: Parasites and Wildlife","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijppaw.2024.101031","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.","name":"copyright","label":"Copyright"}],"article-number":"101031"}}