{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:12:31Z","timestamp":1789755151742,"version":"4.0.1"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["IJID Regions"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.ijregi.2026.100993","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:40:10Z","timestamp":1788277210000},"page":"100993","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Update and optimization of a multiplex RT-qPCR assay to overcome diagnostic failure in emerging influenza A(H3N2) subclades J.2 and K (Peru, 2024\u20132026)"],"prefix":"10.1016","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7023-3190","authenticated-orcid":false,"given":"Miguel Angel","family":"Aguilar-Luis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana del","family":"Valle-Mendoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yordi","family":"Tarazona-Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deysi","family":"Aguilar-Luis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliezer Bonifacio-Velez","family":"de Villa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Cornejo-Tapia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Ant\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Andr\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Laguna-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijregi.2026.100993_bib0001","doi-asserted-by":"crossref","first-page":"210","DOI":"10.3390\/v18020210","article-title":"Genomic evolution of influenza A(H1N1)pdm09 and A\/H3N2 viruses among children in Wuhan, China, spanning the COVID-19 pandemic (2020\u20132023)","volume":"18","author":"Rizwan","year":"2026","journal-title":"Viruses"},{"key":"10.1016\/j.ijregi.2026.100993_bib0002","doi-asserted-by":"crossref","first-page":"3256","DOI":"10.1128\/JCM.40.9.3256-3260.2002","article-title":"Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes","volume":"40","author":"Spackman","year":"2002","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijregi.2026.100993_bib0003","unstructured":"World Health Organization. WHO Information for the molecular detection of influenza viruses. 2024. https:\/\/www.who.int\/teams\/global-influenza-programme\/laboratory-network\/quality-assurance\/eqa-project\/information-for-molecular-diagnosis-of-influenza-virus (Accessed 05 June 2026)"},{"key":"10.1016\/j.ijregi.2026.100993_bib0004","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.3390\/vaccines13121184","article-title":"Evolution and vaccine strain match of HA and NA genes of influenza A\/H3N2 subtype in Riyadh, Saudi Arabia, 2020\u20132023","volume":"13","author":"Alkubaisi","year":"2025","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.ijregi.2026.100993_bib0005","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1186\/s12864-026-12608-y","article-title":"Evolutionary patterns of pH1N1 and H3N2 in relation to vaccine use","volume":"27","author":"Lin","year":"2026","journal-title":"BMC Genomics"},{"key":"10.1016\/j.ijregi.2026.100993_bib0006","unstructured":"Centers for Disease Control and Prevention. Research use only CDC influenza SARS-CoV-2 (Flu SC2) multiplex assay primers, 2021. https:\/\/stacks.cdc.gov\/view\/cdc\/90575"},{"key":"10.1016\/j.ijregi.2026.100993_bib0007","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2025.30.49.2500894","article-title":"Extended influenza seasons in Australia and New Zealand in 2025 due to the emergence of influenza A(H3N2) subclade K viruses","volume":"30","author":"Dapat","year":"2025","journal-title":"Euro Surveill"},{"key":"10.1016\/j.ijregi.2026.100993_bib0008","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1186\/s12879-026-12928-0","article-title":"Genetic characterization of influenza A (A\/H3N2) viruses reveals antigenic drift in receptor binding domain and possible vaccine mismatch in strains circulating in Riyadh, Saudi Arabia, 2024\u20132025","volume":"26","author":"Abdulgader","year":"2026","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.ijregi.2026.100993_bib0009","doi-asserted-by":"crossref","DOI":"10.1155\/av\/3668615","article-title":"Evolving dynamics of whole-genome influenza A\/H3N2 viruses isolated in Cameroon","volume":"2025","author":"Tsafack","year":"2025","journal-title":"Adv Virol"},{"key":"10.1016\/j.ijregi.2026.100993_bib0010","doi-asserted-by":"crossref","first-page":"798","DOI":"10.3390\/v17060798","article-title":"Analysis of epidemiological and evolutionary characteristics of seasonal influenza viruses in Shenzhen City from 2018 to 2024","volume":"17","author":"Peng","year":"2025","journal-title":"Viruses"},{"key":"10.1016\/j.ijregi.2026.100993_bib0011","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00557-20","article-title":"Comparative performance of SARS-CoV-2 detection assays using seven different primer-probe sets and one assay kit","volume":"58","author":"Nalla","year":"2020","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijregi.2026.100993_bib0012","doi-asserted-by":"crossref","DOI":"10.3389\/fviro.2022.840952","article-title":"Investigating the extent of primer dropout in SARS-CoV-2 genome sequences during the early circulation of Delta variants","volume":"2","author":"Borcard","year":"2022","journal-title":"Front Virol"},{"key":"10.1016\/j.ijregi.2026.100993_bib0013","article-title":"Novel introductions of human-origin H3N2 influenza viruses in swine, Chile","volume":"11","author":"Ag\u00fcero","year":"2024","journal-title":"Front Vet Sci"},{"key":"10.1016\/j.ijregi.2026.100993_bib0014","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.3201\/eid2108.150084","article-title":"Phylogeography of influenza A(H3N2) virus in Peru, 2010\u20132012","volume":"21","author":"Pollett","year":"2015","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.ijregi.2026.100993_bib0015","doi-asserted-by":"crossref","first-page":"192","DOI":"10.17843\/rpmesp.2017.342.2054","article-title":"Detection of influenza A, B and subtypes A (H1N1) pdm09, a (H3N2) viruses by multiple QRT-PCR in clinical samples","volume":"34","author":"Marcos","year":"2017","journal-title":"Rev Peru Med Exp Salud Publica"},{"key":"10.1016\/j.ijregi.2026.100993_bib0016","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinf.2024.106164","article-title":"Nanopore sequencing of influenza A and B in Oxfordshire and the United Kingdom, 2022\u201323","volume":"88","author":"Cane","year":"2024","journal-title":"J Infect"},{"key":"10.1016\/j.ijregi.2026.100993_bib0017","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01880-24","article-title":"Improved resolution of avian influenza virus using Oxford nanopore R10 sequencing chemistry","volume":"12","author":"Ratcliff","year":"2024","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.ijregi.2026.100993_bib0018","doi-asserted-by":"crossref","DOI":"10.1111\/irv.70239","article-title":"Strengthening respiratory virus surveillance in sub-Saharan Africa: integrated epidemiological and genomic monitoring in C\u00f4te d\u2019Ivoire","volume":"20","author":"Kadjo","year":"2026","journal-title":"Influenza Other Respir Viruses"},{"key":"10.1016\/j.ijregi.2026.100993_bib0019","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1080\/14787210.2024.2443920","article-title":"Targeting H3N2 influenza: advancements in treatment and vaccine strategies","volume":"23","author":"Srivastava","year":"2025","journal-title":"Expert Rev Anti Infect Ther"},{"key":"10.1016\/j.ijregi.2026.100993_bib0020","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1186\/s12985-025-02765-z","article-title":"Multiplex detection of respiratory RNA viruses without amplification based on CRISPR-Cas13a immunochromatographic test strips","volume":"22","author":"Wang","year":"2025","journal-title":"Virol J"},{"key":"10.1016\/j.ijregi.2026.100993_bib0021","article-title":"Converging influenza threats: seasonal A(H3N2) (including subclade K) dynamics and the persistent pandemic risk of A(H5N1)","volume":"69","author":"Acosta-Espa\u00f1a","year":"2025","journal-title":"Travel Med Infect Dis"}],"container-title":["IJID Regions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2772707626001554?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2772707626001554?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:52:26Z","timestamp":1789753946000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2772707626001554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":21,"alternative-id":["S2772707626001554"],"URL":"https:\/\/doi.org\/10.1016\/j.ijregi.2026.100993","relation":{},"ISSN":["2772-7076"],"issn-type":[{"value":"2772-7076","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Update and optimization of a multiplex RT-qPCR assay to overcome diagnostic failure in emerging influenza A(H3N2) subclades J.2 and K (Peru, 2024\u20132026)","name":"articletitle","label":"Article Title"},{"value":"IJID Regions","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijregi.2026.100993","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.","name":"copyright","label":"Copyright"}],"article-number":"100993"}}