{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:23:23Z","timestamp":1786991003598,"version":"build-2736575974"},"reference-count":86,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002452","name":"Chonbuk National University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002452","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Immunobiology"],"published-print":{"date-parts":[[2019,11]]},"DOI":"10.1016\/j.imbio.2019.09.001","type":"journal-article","created":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T19:08:20Z","timestamp":1567796900000},"page":"747-757","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["An immunoinformatics approach for design and validation of multi-subunit vaccine against Cryptosporidium parvum"],"prefix":"10.1016","volume":"224","author":[{"given":"Ajit Kumar","family":"Dhal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alok","family":"Pani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajani Kanta","family":"Mahapatra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon-IL","family":"Yun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.imbio.2019.09.001_bib0005","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3389\/fimmu.2017.00107","article-title":"Perturbed microRNA expression by Mycobacterium tuberculosis promotes macrophage polarization leading to pro-survival foam cell","volume":"8","author":"Ahluwalia","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0010","doi-asserted-by":"crossref","first-page":"9232","DOI":"10.1038\/s41598-017-09199-w","article-title":"Exploring dengue genome to construct a multi-epitope based subunit vaccine by utilizing immunoinformatics approach to battle against dengue infection","volume":"7","author":"Ali","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.imbio.2019.09.001_bib0015","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.biochi.2015.04.005","article-title":"Orchestration of membrane receptor signaling by membrane lipids","volume":"113","author":"Arish","year":"2015","journal-title":"Biochimie"},{"key":"10.1016\/j.imbio.2019.09.001_bib0020","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0010-4655(95)00042-E","article-title":"GROMACS: a message-passing parallel molecular dynamics implementation","volume":"91","author":"Berendsen","year":"1995","journal-title":"Comput. Phys. Commun."},{"key":"10.1016\/j.imbio.2019.09.001_bib0025","doi-asserted-by":"crossref","first-page":"3195","DOI":"10.1016\/j.vaccine.2004.02.005","article-title":"Prediction of CTL epitopes using QM, SVM and ANN techniques","volume":"22","author":"Bhasin","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.imbio.2019.09.001_bib0030","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1586\/erv.09.160","article-title":"Advances in the design and delivery of peptide subunit vaccines with a focus on toll-like receptor agonists","volume":"9","author":"Black","year":"2010","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.imbio.2019.09.001_bib0035","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.str.2011.02.004","article-title":"The structural biology of Toll-like receptors","volume":"19","author":"Botos","year":"2011","journal-title":"Structure"},{"key":"10.1016\/j.imbio.2019.09.001_bib0040","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.pt.2017.02.006","article-title":"Pathogenic mechanisms of Cryptosporidium and Giardia","volume":"33","author":"Certad","year":"2017","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0045","series-title":"Seminars in Immunology","first-page":"588","article-title":"The role of B cells and humoral immunity in Mycobacterium tuberculosis infection","author":"Chan","year":"2014"},{"key":"10.1016\/j.imbio.2019.09.001_bib0050","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S1473-3099(14)70772-8","article-title":"A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium","volume":"15","author":"Checkley","year":"2015","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.imbio.2019.09.001_bib0055","doi-asserted-by":"crossref","first-page":"7447","DOI":"10.4049\/jimmunol.175.11.7447","article-title":"Multiple TLRs are expressed in human cholangiocytes and mediate host epithelial defense responses to Cryptosporidium parvum via activation of NF-\u03baB","volume":"175","author":"Chen","year":"2005","journal-title":"J. Immunol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0060","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1016\/j.addr.2012.09.039","article-title":"Fusion protein linkers: property, design and functionality","volume":"65","author":"Chen","year":"2013","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.imbio.2019.09.001_bib0065","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1002\/pro.5560020916","article-title":"ERRAT: an empirical atom-based method for validating protein structures","volume":"2","author":"Colovos","year":"1993","journal-title":"Protein Sci."},{"key":"10.1016\/j.imbio.2019.09.001_bib0070","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle mesh Ewald: an N-log (N) method for Ewald sums in large systems","volume":"98","author":"Darden","year":"1993","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.imbio.2019.09.001_bib0075","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1128\/JVI.01505-07","article-title":"Rational design of a multiepitope vaccine encoding T-lymphocyte epitopes for treatment of chronic hepatitis B virus infections","volume":"82","author":"Depla","year":"2008","journal-title":"J. Virol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0080","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1745-6150-8-30","article-title":"Designing of interferon-gamma inducing MHC class-II binders","volume":"8","author":"Dhanda","year":"2013","journal-title":"Biol. Direct"},{"key":"10.1016\/j.imbio.2019.09.001_bib0085","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1186\/1471-2105-14-S6-S4","article-title":"AllerTOP-a server for in silico prediction of allergens","author":"Dimitrov","year":"2013","journal-title":"BMC Bioinf. BioMed Central"},{"key":"10.1016\/j.imbio.2019.09.001_bib0090","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.micpath.2019.05.013","article-title":"In silico analysis and expression of a novel chimeric antigen as a vaccine candidate against Toxoplasma gondii","volume":"132","author":"Dodangeh","year":"2019","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.imbio.2019.09.001_bib0095","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1111\/cmi.12107","article-title":"Cryptosporidium parvum scavenges LDLderived cholesterol and micellar cholesterol internalized into enterocytes","volume":"15","author":"Ehrenman","year":"2013","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0100","series-title":"Methods in Enzymology","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/S0076-6879(97)77022-8","article-title":"VERIFY3D: assessment of protein models with three-dimensional profiles","author":"Eisenberg","year":"1997"},{"key":"10.1016\/j.imbio.2019.09.001_bib0105","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/jmr.893","article-title":"Predicting linear B-cell epitopes using string kernels","volume":"21","author":"EL-Manzalawy","year":"2008","journal-title":"J. Mol. Recognit. Interdiscip. J."},{"issue":"1","key":"10.1016\/j.imbio.2019.09.001_bib0110","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/0471250953.bi0506s15","article-title":"Comparative protein structure modeling using Modeller","volume":"15","author":"Eswar","year":"2006","journal-title":"Curr. Protoc. Bioinf."},{"key":"10.1016\/j.imbio.2019.09.001_bib0115","first-page":"39","article-title":"Past achievements, current situation and future challenges for vaccine development against Cryptosporidium parvum and C. hominis infections","volume":"28","author":"Fereig","year":"2018","journal-title":"J. Protozool. Res."},{"key":"10.1016\/j.imbio.2019.09.001_bib0120","series-title":"The Proteomics Protocols Handbook","first-page":"571","article-title":"Protein identification and analysis tools on the ExPASy server","author":"Gasteiger","year":"2005"},{"key":"10.1016\/j.imbio.2019.09.001_bib0125","doi-asserted-by":"crossref","first-page":"W526","DOI":"10.1093\/nar\/gki376","article-title":"JCat: a novel tool to adapt codon usage of a target gene to its potential expression host","volume":"33","author":"Grote","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.imbio.2019.09.001_bib0130","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.meegid.2016.12.010","article-title":"Immunoinformatics analysis and in silico designing of a novel multi-epitope peptide vaccine against Staphylococcus aureus","volume":"48","author":"Hajighahramani","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0135","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1002\/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H","article-title":"LINCS: a linear constraint solver for molecular simulations","volume":"18","author":"Hess","year":"1997","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.imbio.2019.09.001_bib0140","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.vetpar.2009.12.021","article-title":"Complete development and multiplication of Cryptosporidium hominis in cell-free culture","volume":"169","author":"Hijjawi","year":"2010","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0145","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1002\/prot.21123","article-title":"Comparison of multiple Amber force fields and development of improved protein backbone parameters","volume":"65","author":"Hornak","year":"2006","journal-title":"Proteins Struct. Funct. Bioinf."},{"key":"10.1016\/j.imbio.2019.09.001_bib0150","series-title":"Database 2016","article-title":"Cryptosporidium hominis gene catalog: a resource for the selection of novel Cryptosporidium vaccine candidates","author":"Ifeonu","year":"2016"},{"key":"10.1016\/j.imbio.2019.09.001_bib0155","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/j.cell.2007.09.008","article-title":"Crystal structure of the TLR1-TLR2 heterodimer induced by binding of a tri-acylated lipopeptide","volume":"130","author":"Jin","year":"2007","journal-title":"Cell"},{"key":"10.1016\/j.imbio.2019.09.001_bib0160","doi-asserted-by":"crossref","first-page":"155","DOI":"10.4161\/bbug.1.3.10408","article-title":"Development of a novel oral vaccine against Mycobacterium avium paratuberculosis and Johne disease: a patho-biotechnological approach","volume":"1","author":"Johnston","year":"2010","journal-title":"Bioeng. Bugs"},{"key":"10.1016\/j.imbio.2019.09.001_bib0165","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.exppara.2010.05.008","article-title":"Regulation of CD8+ T cell responses to infection with parasitic protozoa","volume":"126","author":"Jordan","year":"2010","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0170","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1038\/nprot.2012.085","article-title":"Template-based protein structure modeling using the RaptorX web server","volume":"7","author":"K\u00e4llberg","year":"2012","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.imbio.2019.09.001_bib0175","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/j.ijpara.2011.08.001","article-title":"Evolution of Cryptosporidium in vitro culture","volume":"41","author":"Karanis","year":"2011","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0180","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.imbio.2019.09.001_bib0185","doi-asserted-by":"crossref","first-page":"8285","DOI":"10.1038\/s41598-017-08842-w","article-title":"Exploring Leishmania secretory proteins to design B and T cell multi-epitope subunit vaccine using immunoinformatics approach","volume":"7","author":"Khatoon","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.imbio.2019.09.001_bib0190","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.2105\/AJPH.94.11.1931","article-title":"Research and development of new vaccines against infectious diseases","volume":"94","author":"Kieny","year":"2004","journal-title":"Am. J. Public Health"},{"key":"10.1016\/j.imbio.2019.09.001_bib0195","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1016\/j.cell.2007.08.002","article-title":"Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran","volume":"130","author":"Kim","year":"2007","journal-title":"Cell"},{"key":"10.1016\/j.imbio.2019.09.001_bib0200","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0140-6736(13)60844-2","article-title":"Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study","volume":"382","author":"Kotloff","year":"2013","journal-title":"Lancet"},{"key":"10.1016\/j.imbio.2019.09.001_bib0205","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/nprot.2016.169","article-title":"The ClusPro web server for protein\u2013protein docking","volume":"12","author":"Kozakov","year":"2017","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.imbio.2019.09.001_bib0210","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0215","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: a program to check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.imbio.2019.09.001_bib0220","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/j.ijpara.2017.08.001","article-title":"Innate immune responses play a key role in controlling infection of the intestinal epithelium by Cryptosporidium","volume":"47","author":"Laurent","year":"2017","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0225","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.ijpara.2017.10.007","article-title":"RNA-Seq analysis during the life cycle of Cryptosporidium parvum reveals significant differential gene expression between proliferating stages in the intestine and infectious sporozoites","volume":"48","author":"Lippuner","year":"2018","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0230","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1002\/prot.10286","article-title":"Structure validation by C\u03b1 geometry: \u03d5, \u03c8 and C\u03b2 deviation","volume":"50","author":"Lovell","year":"2003","journal-title":"Proteins Struct. Funct. Bioinf."},{"key":"10.1016\/j.imbio.2019.09.001_bib0235","doi-asserted-by":"crossref","first-page":"2592","DOI":"10.1093\/bioinformatics\/btu352","article-title":"SSpro\/ACCpro 5: almost perfect prediction of protein secondary structure and relative solvent accessibility using profiles, machine learning and structural similarity","volume":"30","author":"Magnan","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.imbio.2019.09.001_bib0240","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1128\/CVI.05197-11","article-title":"Identification and immunological characterization of three potential vaccinogens against Cryptosporidium species","volume":"18","author":"Manque","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0245","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.meegid.2017.02.007","article-title":"A novel design of a multi-antigenic, multistage and multi-epitope vaccine against Helicobacter pylori: an in silico approach","volume":"49","author":"Meza","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0250","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.ijpara.2017.10.001","article-title":"A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology","volume":"48","author":"Miller","year":"2018","journal-title":"Int. J. Parasitol."},{"issue":"1","key":"10.1016\/j.imbio.2019.09.001_bib0255","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.ijpara.2015.07.006","article-title":"Continuous culture of Cryptosporidium parvum using hollow fiber technology","volume":"46","author":"Morada","year":"2016","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0260","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/imr.12093","article-title":"The great balancing act: regulation and fate of antiviral Tcell interactions","volume":"255","author":"Moseman","year":"2013","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.imbio.2019.09.001_bib0265","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jtbi.2014.01.018","article-title":"A novel multi-epitope peptide vaccine against cancer: an in silico approach","volume":"349","author":"Nezafat","year":"2014","journal-title":"J. Theor. Biol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0270","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.compbiolchem.2016.04.006","article-title":"Designing an efficient multi-epitope peptide vaccine against Vibrio cholerae via combined immunoinformatics and protein interaction based approaches","volume":"62","author":"Nezafat","year":"2016","journal-title":"Comput. Biol. Chem."},{"key":"10.1016\/j.imbio.2019.09.001_bib0275","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.cvfa.2006.07.002","article-title":"Giardiasis and cryptosporidiosis in ruminants","volume":"22","author":"O\u2019Handley","year":"2006","journal-title":"Vet. Clin. Food Anim. Pract."},{"key":"10.1016\/j.imbio.2019.09.001_bib0280","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1016\/j.vaccine.2018.05.082","article-title":"Development of multi-epitope driven subunit vaccine in secretory and membrane protein of Plasmodium falciparum to convey protection against malaria infection","volume":"36","author":"Pandey","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.imbio.2019.09.001_bib0285","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/s41598-018-19456-1","article-title":"Novel immunoinformatics approaches to design multi-epitope subunit vaccine for malaria by investigating anopheles salivary protein","volume":"8","author":"Pandey","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.imbio.2019.09.001_bib0290","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/07391102.2015.1135298","article-title":"Febrifugine analogues as Leishmania donovani trypanothione reductase inhibitors: binding energy analysis assisted by molecular docking, ADMET and molecular dynamics simulation","volume":"35","author":"Pandey","year":"2017","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.imbio.2019.09.001_bib0295","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1016\/j.vaccine.2018.03.042","article-title":"Immunoinformatics approaches to design a novel multi-epitope subunit vaccine against HIV infection","volume":"36","author":"Pandey","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.imbio.2019.09.001_bib0300","doi-asserted-by":"crossref","first-page":"206","DOI":"10.3389\/fmicb.2016.00206","article-title":"Differential expression of miRNA regulates T cell differentiation and plasticity during visceral leishmaniasis infection","volume":"7","author":"Pandey","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0305","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.biopha.2016.06.010","article-title":"High-throughput virtual screening and quantum mechanics approach to develop imipramine analogues as leads against trypanothione reductase of leishmania","volume":"83","author":"Pandey","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.imbio.2019.09.001_bib0310","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1002\/prot.23175","article-title":"RaptorX: exploiting structure information for protein alignment by statistical inference","volume":"79","author":"Peng","year":"2011","journal-title":"Proteins Struct. Funct. Bioinf."},{"key":"10.1016\/j.imbio.2019.09.001_bib0315","series-title":"Advances in Pharmacology","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/B978-0-12-420118-7.00014-7","article-title":"New directions in nicotine vaccine design and use","author":"Pentel","year":"2014"},{"key":"10.1016\/j.imbio.2019.09.001_bib0320","series-title":"xPharm: The Comprehensive Pharmacology Reference","first-page":"1","author":"Pross","year":"2007"},{"key":"10.1016\/j.imbio.2019.09.001_bib0325","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1080\/21505594.2015.1062211","article-title":"A tug-of-war between the host and the pathogen generates strategic hotspots for the development of novel therapeutic interventions against infectious diseases","volume":"6","author":"Rana","year":"2015","journal-title":"Virulence"},{"key":"10.1016\/j.imbio.2019.09.001_bib0330","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.imbio.2015.12.004","article-title":"A multi-subunit based, thermodynamically stable model vaccine using combined immunoinformatics and protein structure based approach","volume":"221","author":"Rana","year":"2016","journal-title":"Immunobiology"},{"key":"10.1016\/j.imbio.2019.09.001_bib0335","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.mito.2015.05.005","article-title":"Proteome-scale identification and characterization of mitochondria targeting proteins of Mycobacterium avium subspecies paratuberculosis: potential virulence factors modulating host mitochondrial function","volume":"23","author":"Rana","year":"2015","journal-title":"Mitochondrion"},{"key":"10.1016\/j.imbio.2019.09.001_bib0340","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1002\/jmr.2458","article-title":"Proteome wide B and T cell epitope repertoires in outer membrane proteins of Mycobacterium avium subsp. paratuberculosis have vaccine and diagnostic relevance: a holistic approach","volume":"28","author":"Rana","year":"2015","journal-title":"J. Mol. Recognit."},{"key":"10.1016\/j.imbio.2019.09.001_bib0345","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nprot.2010.5","article-title":"I-TASSER: a unified platform for automated protein structure and function prediction","volume":"5","author":"Roy","year":"2010","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.imbio.2019.09.001_bib0350","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.ijpara.2015.01.009","article-title":"New developments in Cryptosporidium research","volume":"45","author":"Ryan","year":"2015","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0355","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.meegid.2017.04.009","article-title":"Development of a multi-epitope peptide vaccine inducing robust T cell responses against brucellosis using immunoinformatics based approaches","volume":"51","author":"Saadi","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0360","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1093\/nar\/gkl343","article-title":"AlgPred: prediction of allergenic proteins and mapping of IgE epitopes","volume":"34","author":"Saha","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.imbio.2019.09.001_bib0365","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/prot.340230306","article-title":"Evaluation of comparative protein modeling by MODELLER","volume":"23","author":"\u0160ali","year":"1995","journal-title":"Proteins Struct. Funct. Bioinf."},{"key":"10.1016\/j.imbio.2019.09.001_bib0370","series-title":"Protein Structure Prediction","first-page":"97","article-title":"Comparative protein structure modeling: introduction and practical examples with modeller","author":"S\u00e1nchez","year":"2000"},{"key":"10.1016\/j.imbio.2019.09.001_bib0375","series-title":"The PyMOL Molecular Graphics System, Version 1.3 r1","author":"Schrodinger","year":"2016"},{"key":"10.1016\/j.imbio.2019.09.001_bib0380","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1093\/nar\/15.3.1281","article-title":"The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications","volume":"15","author":"Sharp","year":"1987","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.imbio.2019.09.001_bib0385","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1038\/503189a","article-title":"Parasitic infections: time to tackle cryptosporidiosis","volume":"503","author":"Striepen","year":"2013","journal-title":"Nat. News"},{"key":"10.1016\/j.imbio.2019.09.001_bib0390","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.2217\/fvl.12.108","article-title":"Role of T-cell epitope-based vaccine in prophylactic and therapeutic applications","volume":"7","author":"Testa","year":"2012","journal-title":"Future Virol."},{"issue":"7","key":"10.1016\/j.imbio.2019.09.001_bib0395","article-title":"Graphing with gnuplot and xmgr: two graphing packages available under linux","volume":"1996","author":"Vaught","year":"1996","journal-title":"Linux J."},{"key":"10.1016\/j.imbio.2019.09.001_bib0400","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/protein\/8.2.127","article-title":"LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions","volume":"8","author":"Wallace","year":"1995","journal-title":"Protein Eng. Des. Sel."},{"key":"10.1016\/j.imbio.2019.09.001_bib0405","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1000048","article-title":"A systematic assessment of MHC class II peptide binding predictions and evaluation of a consensus approach","volume":"4","author":"Wang","year":"2008","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.imbio.2019.09.001_bib0410","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1186\/1471-2105-11-568","article-title":"Peptide binding predictions for HLA DR, DP and DQ molecules","volume":"11","author":"Wang","year":"2010","journal-title":"BMC Bioinf."},{"issue":"1","key":"10.1016\/j.imbio.2019.09.001_bib0415","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/0471250953.bi0506s47","article-title":"Comparative protein structure modeling using MODELLER","volume":"47","author":"Webb","year":"2014","journal-title":"Curr. Protoc. Bioinf."},{"key":"10.1016\/j.imbio.2019.09.001_bib0420","doi-asserted-by":"crossref","first-page":"W407","DOI":"10.1093\/nar\/gkm290","article-title":"ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins","volume":"35","author":"Wiederstein","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.imbio.2019.09.001_bib0425","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.1093\/nar\/gkm251","article-title":"LOMETS: a local meta-threading-server for protein structure prediction","volume":"35","author":"Wu","year":"2007","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.imbio.2019.09.001_bib0430","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1471-2105-9-40","article-title":"I-TASSER server for protein 3D structure prediction","volume":"9","author":"Zhang","year":"2008","journal-title":"BMC Bioinf."}],"container-title":["Immunobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0171298519300956?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0171298519300956?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T16:45:04Z","timestamp":1759250704000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0171298519300956"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":86,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["S0171298519300956"],"URL":"https:\/\/doi.org\/10.1016\/j.imbio.2019.09.001","relation":{},"ISSN":["0171-2985"],"issn-type":[{"value":"0171-2985","type":"print"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An immunoinformatics approach for design and validation of multi-subunit vaccine against Cryptosporidium parvum","name":"articletitle","label":"Article Title"},{"value":"Immunobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.imbio.2019.09.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}