{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T18:26:47Z","timestamp":1793730407918,"version":"4.6.1"},"reference-count":31,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010814","name":"Department of Education of Anhui Province","doi-asserted-by":"publisher","award":["2023AH053296"],"award-info":[{"award-number":["2023AH053296"]}],"id":[{"id":"10.13039\/501100010814","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003995","name":"Anhui Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["2408085MH209"],"award-info":[{"award-number":["2408085MH209"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Immunobiology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.imbio.2026.153225","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:04:25Z","timestamp":1786115065000},"page":"153225","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["The effect of storage time on lymphocytes function detection: Application in clinical samples"],"prefix":"10.1016","volume":"231","author":[{"given":"Ruoyu","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoran","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanhong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanling","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.imbio.2026.153225_bb0005","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.cellimm.2008.08.007","article-title":"Relationship between CD107a expression and cytotoxic activity","volume":"254","author":"Aktas","year":"2009","journal-title":"Cell. Immunol."},{"key":"10.1016\/j.imbio.2026.153225_bb0010","doi-asserted-by":"crossref","first-page":"2493","DOI":"10.3389\/fimmu.2019.02493","article-title":"NKT-like (CD3+CD56+) cells in chronic myeloid Leukemia patients treated with tyrosine kinase inhibitors","volume":"10","author":"Almeida","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.imbio.2026.153225_bb0015","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.cytogfr.2009.02.004","article-title":"Interferon-gamma: a historical perspective","volume":"20","author":"Billiau","year":"2009","journal-title":"Cytokine Growth Factor Rev."},{"key":"10.1016\/j.imbio.2026.153225_bb0020","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/s41577-021-00558-3","article-title":"Natural killer cells in antiviral immunity","volume":"22","author":"Bjorkstrom","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.imbio.2026.153225_bb0025","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1111\/j.1600-0897.2004.00177.x","article-title":"Gender difference in the non-specific and specific immune response in humans","volume":"52","author":"Bouman","year":"2004","journal-title":"Am. J. Reprod. Immunol."},{"key":"10.1016\/j.imbio.2026.153225_bb0030","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1038\/sj.cdd.4401171","article-title":"TGF-beta1 suppresses apoptosis via differential regulation of MAP kinases and ceramide production","volume":"10","author":"Chen","year":"2003","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.imbio.2026.153225_bb0035","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/j.1365-2141.2008.07028.x","article-title":"Absolute lymphocyte count is a prognostic factor in diffuse large B-cell lymphoma","volume":"141","author":"Cox","year":"2008","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.imbio.2026.153225_bb0040","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1373\/clinchem.2014.228783","article-title":"Preanalytical variables affecting the integrity of human biospecimens in biobanking","volume":"61","author":"Ellervik","year":"2015","journal-title":"Clin. Chem."},{"key":"10.1016\/j.imbio.2026.153225_bb0045","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.00230-25","article-title":"The inhibitory receptor LAG3 affects NK cell IFN-\u03b3 production through glycolysis and the PSAT1\/STAT1\/IFNG pathway","volume":"16","author":"Ge","year":"2025","journal-title":"mBio"},{"key":"10.1016\/j.imbio.2026.153225_bb0050","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1002\/cyto.a.20048","article-title":"Distinguishing modes of cell death using the ImageStream multispectral imaging flow cytometer","volume":"59","author":"George","year":"2004","journal-title":"Cytometry A"},{"key":"10.1016\/j.imbio.2026.153225_bb0055","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nri1309","article-title":"NKT cells: what\u2019s in a name?","volume":"4","author":"Godfrey","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.imbio.2026.153225_bb0060","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s12967-017-1154-3","article-title":"Stability of pro- and anti-inflammatory immune biomarkers for human cohort studies","volume":"15","author":"Graham","year":"2017","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.imbio.2026.153225_bb0065","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.cyto.2017.05.014","article-title":"Effect of the anticoagulant, storage time and temperature of blood samples on the concentrations of 27 multiplex assayed cytokines - consequences for defining reference values in healthy humans","volume":"97","author":"Henno","year":"2017","journal-title":"Cytokine"},{"key":"10.1016\/j.imbio.2026.153225_bb0070","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1186\/s12890-022-02137-1","article-title":"Prognostic value of lymphocyte count for in-hospital mortality in patients with severe AECOPD","volume":"22","author":"Hu","year":"2022","journal-title":"BMC Pulm. Med."},{"key":"10.1016\/j.imbio.2026.153225_bb0075","doi-asserted-by":"crossref","DOI":"10.1042\/BSR20203827","article-title":"Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples","volume":"41","author":"Jerram","year":"2021","journal-title":"Biosci. Rep."},{"key":"10.1016\/j.imbio.2026.153225_bb0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2021.113900","article-title":"Stability of interleukin-1beta, \u22124, \u22126, \u22128, \u221210, \u221213, interferon-gamma and tumor necrosis factor-alpha in human sera after repetitive freeze-thaw cycles and long storage","volume":"196","author":"Kordulewska","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.imbio.2026.153225_bb0085","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.pathol.2023.11.011","article-title":"Effects of blood sample storage time, temperature, anti-coagulants and blood stabiliser on lymphocyte phenotyping","volume":"56","author":"Lao","year":"2024","journal-title":"Pathology"},{"key":"10.1016\/j.imbio.2026.153225_bb0090","article-title":"Cytokines: from clinical significance to quantification","volume":"8","author":"Liu","year":"2021","journal-title":"Adv. Sci. (Weinh)"},{"key":"10.1016\/j.imbio.2026.153225_bb0095","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1002\/art.42435","article-title":"Down-regulation-resistant STAT4 risk haplotype contributes to lupus nephritis through CD4+ T cell interferon-gamma production","volume":"75","author":"Madera-Salcedo","year":"2023","journal-title":"Arthritis Rheumatol."},{"key":"10.1016\/j.imbio.2026.153225_bb0100","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1097\/RLI.0000000000000752","article-title":"Acute chelation therapy-associated changes in urine gadolinium, self-reported flare severity, and serum cytokines in gadolinium deposition disease","volume":"56","author":"Maecker","year":"2021","journal-title":"Investig. Radiol."},{"key":"10.1016\/j.imbio.2026.153225_bb0105","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.1084\/jem.20070401","article-title":"High basal STAT4 balanced by STAT1 induction to control type 1 interferon effects in natural killer cells","volume":"204","author":"Miyagi","year":"2007","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.imbio.2026.153225_bb0110","doi-asserted-by":"crossref","first-page":"2063","DOI":"10.1126\/science.1074900","article-title":"Critical role for STAT4 activation by type 1 interferons in the interferon-gamma response to viral infection","volume":"297","author":"Nguyen","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.imbio.2026.153225_bb0115","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.cyto.2007.03.006","article-title":"Effect of storage temperatures on the stability of cytokines in cervical mucous","volume":"37","author":"Panicker","year":"2007","journal-title":"Cytokine"},{"key":"10.1016\/j.imbio.2026.153225_bb0120","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.coi.2009.05.008","article-title":"Diversity in CD8(+) T cell differentiation","volume":"21","author":"Parish","year":"2009","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.imbio.2026.153225_bb0125","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1002\/eji.200425797","article-title":"Direct effects of T-bet and MHC class I expression, but not STAT1, on peripheral NK cell maturation","volume":"35","author":"Robbins","year":"2005","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.imbio.2026.153225_bb0130","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1038\/s41423-019-0220-6","article-title":"Helper T cell differentiation","volume":"16","author":"Saravia","year":"2019","journal-title":"Cell. Mol. Immunol."},{"key":"10.1016\/j.imbio.2026.153225_bb0135","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24010634","article-title":"Quantifying the detrimental effects of multiple freeze\/thaw cycles on primary human lymphocyte survival and function","volume":"24","author":"Serra","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.imbio.2026.153225_bb0140","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/382171a0","article-title":"Requirement for Stat4 in interleukin-12-mediated responses of natural killer and T cells","volume":"382","author":"Thierfelder","year":"1996","journal-title":"Nature"},{"key":"10.1016\/j.imbio.2026.153225_bb0145","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.cell.2012.09.044","article-title":"STATs Shape the active enhancer landscape of T cell populations","volume":"151","author":"Vahedi","year":"2012","journal-title":"Cell"},{"key":"10.1016\/j.imbio.2026.153225_bb0150","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1515\/cclm-2013-0705","article-title":"Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma","volume":"52","author":"Zander","year":"2014","journal-title":"Clin. Chem. Lab. Med."},{"key":"10.1016\/j.imbio.2026.153225_bb0155","doi-asserted-by":"crossref","DOI":"10.1186\/s13046-018-0764-9","article-title":"Attenuation of TGFBR2 expression and tumour progression in prostate cancer involve diverse hypoxia-regulated pathways","volume":"37","author":"Zhou","year":"2018","journal-title":"J. Exp. Clin. Cancer Res."}],"container-title":["Immunobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0171298526000719?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0171298526000719?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:31:16Z","timestamp":1793727076000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0171298526000719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S0171298526000719"],"URL":"https:\/\/doi.org\/10.1016\/j.imbio.2026.153225","relation":{},"ISSN":["0171-2985"],"issn-type":[{"value":"0171-2985","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The effect of storage time on lymphocytes function detection: Application in clinical samples","name":"articletitle","label":"Article Title"},{"value":"Immunobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.imbio.2026.153225","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier GmbH.","name":"copyright","label":"Copyright"}],"article-number":"153225"}}