{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:31:11Z","timestamp":1790469071020,"version":"4.1.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Immunology Letters"],"published-print":{"date-parts":[[2016,5]]},"DOI":"10.1016\/j.imlet.2016.03.005","type":"journal-article","created":{"date-parts":[[2016,3,11]],"date-time":"2016-03-11T17:35:58Z","timestamp":1457717758000},"page":"61-68","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":38,"special_numbering":"C","title":["Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum"],"prefix":"10.1016","volume":"173","author":[{"given":"M\u00f3nica M.","family":"Castiblanco-Valencia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana R.","family":"Fraga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro C.D.","family":"Breda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00edlvio A.","family":"Vasconcellos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udio P.","family":"Figueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsio","family":"Wunder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert I.","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela S.","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Isaac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.imlet.2016.03.005_bib0005","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.ijid.2007.09.011","article-title":"The globalization of leptospirosis: worldwide incidence trends","volume":"12","author":"Pappas","year":"2008","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.imlet.2016.03.005_bib0010","doi-asserted-by":"crossref","first-page":"e228","DOI":"10.1371\/journal.pntd.0000228","article-title":"Impact of environment and social gradient on Leptospira infection in urban slums","volume":"2","author":"Reis","year":"2008","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.imlet.2016.03.005_bib0015","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1111\/j.1469-0691.2011.03474.x","article-title":"Emergence: control and re-emerging leptospirosis: dynamics of infection in the changing world","volume":"17","author":"Hartskeerl","year":"2011","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.imlet.2016.03.005_bib0020","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1111\/j.1365-3083.2010.02505.x","article-title":"Leptospirosis: aspects of innate immunity: immunopathogenesis and immune evasion from the complement system","volume":"73","author":"Fraga","year":"2011","journal-title":"Scand. J. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0025","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1128\/IAI.01310-08","article-title":"Immune evasion of leptospira species by acquisition of human complement regulator C4BP","volume":"77","author":"Barbosa","year":"2009","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0030","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.micpath.2005.07.003","article-title":"Regulation of complement activation at the C3-level by serum resistant leptospires","volume":"39","author":"Meri","year":"2005","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.imlet.2016.03.005_bib0035","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1093\/infdis\/jit569","article-title":"Immune evasion by pathogenic Leptospira strains: the secretion of proteases that directly cleave complement proteins","volume":"209","author":"Fraga","year":"2014","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.imlet.2016.03.005_bib0040","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1084\/jem.144.5.1147","article-title":"Modulation of the alternative complement pathways by beta 1H globulin","volume":"144","author":"Whaley","year":"1976","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.imlet.2016.03.005_bib0045","doi-asserted-by":"crossref","first-page":"3268","DOI":"10.1073\/pnas.73.9.3268","article-title":"Control of the amplification convertase of complement by the plasma protein beta1H","volume":"73","author":"Weiler","year":"1976","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.imlet.2016.03.005_bib0050","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1084\/jem.146.1.257","article-title":"Human complement C3b inactivator: isolation characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution","volume":"146","author":"Pangburn","year":"1977","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.imlet.2016.03.005_bib0055","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1093\/infdis\/jir875","article-title":"Leptospiral immunoglobulin-like proteins interact with human complement regulators factor H, FHL-1 FHR-1, and C4BP","volume":"205","author":"Castiblanco-Valencia","year":"2012","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.imlet.2016.03.005_bib0060","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1084\/jem.148.1.207","article-title":"Human C4-binding protein. I. isolation and characterization","volume":"148","author":"Scharfstein","year":"1978","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.imlet.2016.03.005_bib0065","doi-asserted-by":"crossref","first-page":"6596","DOI":"10.1073\/pnas.76.12.6596","article-title":"Modulation of the classical pathway C3 convertase by plasma proteins C4 binding protein and C3b inactivator","volume":"76","author":"Gigli","year":"1979","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.imlet.2016.03.005_bib0070","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1128\/IAI.01619-06","article-title":"Response of Leptospira interrogans to physiologic osmolarity: relevance in signaling the environment-to-host transition","volume":"75","author":"Matsunaga","year":"2007","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0075","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1128\/IAI.73.1.70-78.2005","article-title":"Osmolarity: a key environmental signal controlling expression of leptospiral proteins LigA and LigB and the extracellular release of LigA","volume":"73","author":"Matsunaga","year":"2005","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0080","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1128\/IAI.01635-06","article-title":"Physiological osmotic induction of Leptospira interrogans adhesion: ligA and LigB bind extracellular matrix proteins and fibrinogen","volume":"75","author":"Choy","year":"2007","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0085","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.bbrc.2007.07.196","article-title":"A domain of the Leptospira LigB contributes to high affinity binding of fibronectin","volume":"362","author":"Lin","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.imlet.2016.03.005_bib0090","doi-asserted-by":"crossref","first-page":"133","DOI":"10.4142\/jvs.2008.9.2.133","article-title":"The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin","volume":"9","author":"Lin","year":"2008","journal-title":"J. Vet. Sci."},{"key":"10.1016\/j.imlet.2016.03.005_bib0095","doi-asserted-by":"crossref","first-page":"23547","DOI":"10.1074\/jbc.M109.031369","article-title":"Fibronectin binds to and induces conformational change in a disordered region of leptospiral immunoglobulin-like protein B","volume":"284","author":"Lin","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.imlet.2016.03.005_bib0100","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.bbrc.2009.08.089","article-title":"A novel fibronectin type III module binding motif identified on C-terminus of Leptospira immunoglobulin-like protein","volume":"389","author":"Lin","year":"2009","journal-title":"LigB. Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.imlet.2016.03.005_bib0105","doi-asserted-by":"crossref","first-page":"19380","DOI":"10.1074\/jbc.M109.004531","article-title":"Repeated domains of leptospira immunoglobulin-like proteins interact with elastin and tropoelastin","volume":"284","author":"Lin","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.imlet.2016.03.005_bib0110","doi-asserted-by":"crossref","first-page":"e11301","DOI":"10.1371\/journal.pone.0011301","article-title":"The terminal immunoglobulin-like repeats of LigA and LigB of Leptospira enhance their binding to gelatin binding domain of fibronectin and host cells","volume":"5","author":"Lin","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.imlet.2016.03.005_bib0115","doi-asserted-by":"crossref","first-page":"25140","DOI":"10.1074\/jbc.M801350200","article-title":"Calcium binds to leptospiral immunoglobulin-like protein LigB, and modulates fibronectin binding","volume":"283","author":"Lin","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.imlet.2016.03.005_bib0120","doi-asserted-by":"crossref","first-page":"5924","DOI":"10.1128\/IAI.70.11.5924-5930.2002","article-title":"Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans","volume":"70","author":"Palaniappan","year":"2002","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0125","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1046\/j.1365-2958.2003.03619.x","article-title":"Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily","volume":"49","author":"Matsunaga","year":"2003","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0130","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1128\/JCM.02344-06","article-title":"Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis","volume":"45","author":"Croda","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0135","doi-asserted-by":"crossref","first-page":"6277","DOI":"10.1016\/j.vaccine.2007.05.053","article-title":"The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis","volume":"25","author":"Silva","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.imlet.2016.03.005_bib0140","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.vaccine.2007.10.029","article-title":"Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters","volume":"26","author":"Faisal","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.imlet.2016.03.005_bib0145","doi-asserted-by":"crossref","first-page":"5826","DOI":"10.1128\/IAI.00989-08","article-title":"Targeted mutagenesis in pathogenic Leptospira species: disruption of the LigB gene does not affect virulence in animal models of leptospirosis","volume":"76","author":"Croda","year":"2008","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0150","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/1471-2180-11-129","article-title":"Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin","volume":"11","author":"Figueira","year":"2011","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0155","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1128\/JB.89.6.1625-1626.1965","article-title":"Antileptospiral activity of normal serum","volume":"89","author":"Johnson","year":"1965","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0160","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1128\/JB.93.2.513-519.1967","article-title":"Antileptospiral activity of serum. II. Leptospiral virulence factor","volume":"93","author":"Johnson","year":"1967","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0165","doi-asserted-by":"crossref","first-page":"2659","DOI":"10.1128\/IAI.74.5.2659-2666.2006","article-title":"LfhA: a novel factor H-binding protein of Leptospira interrogans","volume":"74","author":"Verma","year":"2006","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0170","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1128\/IAI.02844-14","article-title":"Pathogenic Leptospira species acquire factor H and vitronectin via the surface protein LcpA","volume":"83","author":"da Silva","year":"2015","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0175","doi-asserted-by":"crossref","first-page":"e1188","DOI":"10.1371\/journal.pone.0001188","article-title":"Leptospira interrogans endostatin-like outer membrane proteins bind host fibronectin, laminin and regulators of complement","volume":"2","author":"Stevenson","year":"2007","journal-title":"PLoS One"},{"key":"10.1016\/j.imlet.2016.03.005_bib0180","doi-asserted-by":"crossref","first-page":"3207","DOI":"10.1128\/IAI.00279-10","article-title":"Functional characterization of LcpA: a surface-exposed protein of Leptospira spp. that binds the human complement regulator C4BP","volume":"78","author":"Barbosa","year":"2010","journal-title":"Infect. Immunol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0185","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.meegid.2008.10.012","article-title":"Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp","volume":"9","author":"McBride","year":"2009","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.imlet.2016.03.005_bib0190","doi-asserted-by":"crossref","first-page":"7888","DOI":"10.1128\/AEM.02202-15","article-title":"Control of gene expression in leptospira spp by transcription activator-Like effectors demonstrates a potential role for LigA and LigB in Leptospira interrogans virulence","volume":"81","author":"Pappas","year":"2015","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Immunology Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016524781630030X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016524781630030X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,15]],"date-time":"2021-05-15T10:15:22Z","timestamp":1621073722000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016524781630030X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":38,"alternative-id":["S016524781630030X"],"URL":"https:\/\/doi.org\/10.1016\/j.imlet.2016.03.005","relation":{},"ISSN":["0165-2478"],"issn-type":[{"value":"0165-2478","type":"print"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum","name":"articletitle","label":"Article Title"},{"value":"Immunology Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.imlet.2016.03.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}