{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T03:56:43Z","timestamp":1793332603090,"version":"4.5.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC81971511"],"award-info":[{"award-number":["NSFC81971511"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82471187"],"award-info":[{"award-number":["82471187"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017534","name":"Project 333 of Jiangsu Province","doi-asserted-by":"publisher","award":["ZUZHIBU202221001"],"award-info":[{"award-number":["ZUZHIBU202221001"]}],"id":[{"id":"10.13039\/501100017534","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["821MS145"],"award-info":[{"award-number":["821MS145"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Immunopharmacology"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.intimp.2025.115833","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T06:24:57Z","timestamp":1763619897000},"page":"115833","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"P1","title":["Genome-wide characterization of Blomia tropicalis allergenome: chromosome-level assembly, bioinformatics insights, and clinical validation of novel allergens"],"prefix":"10.1016","volume":"168","author":[{"given":"Ying","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinni","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel B.","family":"Klimov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaning","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanfen","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cunyin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianglong","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoda","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Ping","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yubao","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.intimp.2025.115833_bb0005","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1111\/j.1365-2222.2012.04035.x","article-title":"Component-resolved diagnosis with commercially available D. Pteronyssinus Der p 1, Der p 2 and Der p 10: relevant markers for house dust mite allergy","volume":"42","author":"Bronnert","year":"2012","journal-title":"Clin. Exp. Allergy"},{"issue":"14","key":"10.1016\/j.intimp.2025.115833_bb0010","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.2217\/imt-2023-0051","article-title":"A novel dose-adjustment protocol for interrupted subcutaneous immunotherapy for allergic rhinitis","volume":"15","author":"Feng","year":"2023","journal-title":"Immunotherapy"},{"key":"10.1016\/j.intimp.2025.115833_bb0015","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s12948-018-0092-9","article-title":"House dust mite-related respiratory allergies and probiotics: a narrative review","volume":"16","author":"Fassio","year":"2018","journal-title":"Clin. Mol. Allergy"},{"key":"10.1016\/j.intimp.2025.115833_bb0020","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.molimm.2023.04.008","article-title":"House dust mite allergy: the importance of house dust mite allergens for diagnosis and immunotherapy","volume":"158","author":"Huang","year":"2023","journal-title":"Mol. Immunol."},{"issue":"8","key":"10.1016\/j.intimp.2025.115833_bb0025","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23084297","article-title":"Storage mite precision allergy molecular diagnosis in the moderate-to-severe T2-high asthma phenotype","volume":"23","author":"Gonzalez-Perez","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"4","key":"10.1016\/j.intimp.2025.115833_bb0030","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1159\/000464325","article-title":"Allergens of Blomia tropicalis: an overview of recombinant molecules","volume":"172","author":"Santos da Silva","year":"2017","journal-title":"Int. Arch. Allergy Immunol."},{"issue":"2","key":"10.1016\/j.intimp.2025.115833_bb0035","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1111\/j.1399-3038.2010.01094.x","article-title":"Mite component-specific IgE repertoire and phenotypes of allergic disease in childhood: the tropical perspective","volume":"22","author":"Kidon","year":"2011","journal-title":"Pediatr. Allergy Immunol."},{"issue":"3","key":"10.1016\/j.intimp.2025.115833_bb0040","first-page":"127","article-title":"Distribution of allergens in children with different atopic disorders in Central Taiwan","volume":"47","author":"Huang","year":"2006","journal-title":"Acta Paediatr. Taiwan."},{"key":"10.1016\/j.intimp.2025.115833_bb0045","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1471-2172-14-11","article-title":"Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract","volume":"14","author":"Carvalho Kdos","year":"2013","journal-title":"BMC Immunol."},{"issue":"3","key":"10.1016\/j.intimp.2025.115833_bb0050","doi-asserted-by":"crossref","first-page":"157","DOI":"10.18176\/jiaci.0892","article-title":"IgE-mediated sensitization to Blo t 21 and Blo t 5 is associated with asthma in the tropics: a case-control study","volume":"34","author":"Zakzuk","year":"2024","journal-title":"J Investig Allergol Clin Immunol"},{"issue":"4 Pt 2","key":"10.1016\/j.intimp.2025.115833_bb0055","doi-asserted-by":"crossref","first-page":"e718","DOI":"10.1111\/j.1399-3038.2010.01019.x","article-title":"Specific profiles of house dust mite sensitization in children with asthma and in children with eczema","volume":"21","author":"Shek","year":"2010","journal-title":"Pediatr. Allergy Immunol."},{"issue":"7","key":"10.1016\/j.intimp.2025.115833_bb0060","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1111\/j.1398-9995.2009.01967.x","article-title":"A multicentre study assessing the prevalence of sensitizations in patients with asthma and\/or rhinitis in China","volume":"64","author":"J. Li, B. Sun, Y. Huang, X. Lin, D. Zhao, G. Tan, J. Wu, H. Zhao, L. Cao, N. Zhong, D. China Alliance of Research on Respiratory Allergic","year":"2009","journal-title":"Allergy"},{"issue":"5","key":"10.1016\/j.intimp.2025.115833_bb0065","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1177\/1945892418787116","article-title":"The Dermatophagoides pteronyssinus molecular sensitization profile of allergic rhinitis patients in Central China","volume":"32","author":"Yang","year":"2018","journal-title":"Am. J. Rhinol. Allergy"},{"issue":"10","key":"10.1016\/j.intimp.2025.115833_bb0070","doi-asserted-by":"crossref","DOI":"10.1002\/clt2.12302","article-title":"Predicting Blomia tropicalis allergens using a multiomics approach","volume":"13","author":"Hubert","year":"2023","journal-title":"Clin. Transl. Allergy"},{"issue":"2","key":"10.1016\/j.intimp.2025.115833_bb0075","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.alit.2023.11.004","article-title":"Genomic analysis reveals novel allergens of Blomia tropicalis","volume":"73","author":"Xiong","year":"2024","journal-title":"Allergol. Int."},{"key":"10.1016\/j.intimp.2025.115833_bb0080","doi-asserted-by":"crossref","DOI":"10.1155\/2024\/3779688","article-title":"A chromosomal-level genome of Dermatophagoides farinae, a common allergenic mite species","volume":"2024","author":"Hu","year":"2024","journal-title":"Int. J. Genomics"},{"issue":"6","key":"10.1016\/j.intimp.2025.115833_bb0085","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1111\/all.15656","article-title":"Chromosome-level genomic assembly and allergome inference reveal novel allergens in Tyrophagus putrescentiae","volume":"78","author":"Zhou","year":"2023","journal-title":"Allergy"},{"issue":"6","key":"10.1016\/j.intimp.2025.115833_bb0090","first-page":"3551","article-title":"Identification of three aquaporin subgroups from Blomia tropicalis by transcriptomics","volume":"42","author":"Zhou","year":"2018","journal-title":"Int. J. Mol. Med."},{"issue":"9","key":"10.1016\/j.intimp.2025.115833_bb0095","first-page":"636","article-title":"Chinese guidelines for the diagnosis and treatment of allergic asthma (2019, the first edition)","volume":"58","year":"2019","journal-title":"Zhonghua Nei Ke Za Zhi"},{"issue":"6","key":"10.1016\/j.intimp.2025.115833_bb0100","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1093\/bioinformatics\/btr011","article-title":"A fast, lock-free approach for efficient parallel counting of occurrences of k-mers","volume":"27","author":"Mar\u00e7ais","year":"2011","journal-title":"Bioinformatics"},{"issue":"12","key":"10.1016\/j.intimp.2025.115833_bb0105","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1093\/bioinformatics\/btr174","article-title":"BamTools: a C++ API and toolkit for analyzing and managing BAM files","volume":"27","author":"Barnett","year":"2011","journal-title":"Bioinformatics"},{"issue":"10","key":"10.1016\/j.intimp.2025.115833_bb0110","doi-asserted-by":"crossref","first-page":"4647","DOI":"10.1093\/molbev\/msab199","article-title":"BUSCO update: novel and streamlined workflows along with broader and deeper phylogenetic coverage for scoring of eukaryotic. Prokaryotic, and viral genomes","volume":"38","author":"Manni","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.intimp.2025.115833_bb0115","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ymeth.2019.09.012","article-title":"Sci-hi-C: a single-cell hi-C method for mapping 3D genome organization in large number of single cells","volume":"170","author":"Ramani","year":"2020","journal-title":"Methods"},{"issue":"2","key":"10.1016\/j.intimp.2025.115833_bb0120","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.cels.2018.01.001","article-title":"Juicebox.Js provides a cloud-based visualization system for hi-C data","volume":"6","author":"Robinson","year":"2018","journal-title":"Cell Syst."},{"key":"10.1016\/j.intimp.2025.115833_bb0125","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.12688\/f1000research.7334.1","article-title":"HiCUP: pipeline for mapping and processing Hi-C data","volume":"4","author":"Wingett","year":"2015","journal-title":"F1000Res"},{"issue":"3","key":"10.1016\/j.intimp.2025.115833_bb0130","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.intimp.2025.115833_bb0135","doi-asserted-by":"crossref","DOI":"10.12688\/f1000research.23297.1","article-title":"GFF Utilities: GffRead and GffCompare","volume":"9","author":"Pertea","year":"2020","journal-title":"F1000Res"},{"issue":"2","key":"10.1016\/j.intimp.2025.115833_bb0140","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/all.15111","article-title":"Regulatory roles of three miRNAs on allergen mRNA expression in Tyrophagus putrescentiae","volume":"77","author":"Zhou","year":"2022","journal-title":"Allergy"},{"issue":"Database issue","key":"10.1016\/j.intimp.2025.115833_bb0145","doi-asserted-by":"crossref","first-page":"D121","DOI":"10.1093\/nar\/gki081","article-title":"Rfam: annotating non-coding RNAs in complete genomes","volume":"33","author":"Griffiths-Jones","year":"2005","journal-title":"Nucleic Acids Res."},{"issue":"16","key":"10.1016\/j.intimp.2025.115833_bb0150","doi-asserted-by":"crossref","first-page":"9077","DOI":"10.1093\/nar\/gkab688","article-title":"Lowe, tRNAscan-SE 2.0: improved detection and functional classification of transfer RNA genes","volume":"49","author":"Chan","year":"2021","journal-title":"Nucleic Acids Res."},{"issue":"9","key":"10.1016\/j.intimp.2025.115833_bb0155","doi-asserted-by":"crossref","first-page":"3100","DOI":"10.1093\/nar\/gkm160","article-title":"RNAmmer: consistent and rapid annotation of ribosomal RNA genes","volume":"35","author":"Lagesen","year":"2007","journal-title":"Nucleic Acids Res."},{"issue":"Pt 1","key":"10.1016\/j.intimp.2025.115833_bb0160","article-title":"Expression, purification, and activity of novel allergen Tyr p 31 from Tyrophagus putrescentiae","volume":"258","author":"Zhou","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"Pt 3","key":"10.1016\/j.intimp.2025.115833_bb0165","article-title":"Immunobiological properties and structure analysis of group 13 allergen from Blomia tropicalis and its IgE-mediated cross-reactivity","volume":"254","author":"Zhou","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"4","key":"10.1016\/j.intimp.2025.115833_bb0170","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S1389-1723(02)80166-9","article-title":"Toward elucidating the full spectrum of mite allergens--state of the art","volume":"94","author":"Kawamoto","year":"2002","journal-title":"J. Biosci. Bioeng."},{"issue":"1","key":"10.1016\/j.intimp.2025.115833_bb0175","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s10493-022-00745-4","article-title":"Complete mitochondrial genomes of Thyreophagus entomophagus and Acarus siro (Sarcoptiformes: Astigmatina) provide insight into mitogenome features, evolution, and phylogeny among Acaroidea mites","volume":"88","author":"Fang","year":"2022","journal-title":"Exp. Appl. Acarol."},{"issue":"6","key":"10.1016\/j.intimp.2025.115833_bb0180","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24065543","article-title":"The allergenic activity of Blo t 2, a Blomia tropicalis IgE-binding molecule","volume":"24","author":"Mondol","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.intimp.2025.115833_bb0185","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.651500","article-title":"Are the terms major and minor allergens useful for precision Allergology?","volume":"12","author":"Caraballo","year":"2021","journal-title":"Front. Immunol."},{"issue":"4","key":"10.1016\/j.intimp.2025.115833_bb0190","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.alit.2015.05.004","article-title":"Hierarchy and molecular properties of house dust mite allergens","volume":"64","author":"Thomas","year":"2015","journal-title":"Allergol. Int."}],"container-title":["International Immunopharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567576925018211?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567576925018211?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T19:43:17Z","timestamp":1781898197000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1567576925018211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":38,"alternative-id":["S1567576925018211"],"URL":"https:\/\/doi.org\/10.1016\/j.intimp.2025.115833","relation":{},"ISSN":["1567-5769"],"issn-type":[{"value":"1567-5769","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genome-wide characterization of Blomia tropicalis allergenome: chromosome-level assembly, bioinformatics insights, and clinical validation of novel allergens","name":"articletitle","label":"Article Title"},{"value":"International Immunopharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.intimp.2025.115833","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"115833"}}