{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:26:48Z","timestamp":1790864808752,"version":"4.1.0"},"reference-count":75,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000958","name":"American Association for the Advancement of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000958","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001082","name":"Camille and Henry Dreyfus Foundation Inc","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001082","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.isci.2022.104443","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T17:26:23Z","timestamp":1653326783000},"page":"104443","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["A single amino acid residue controls acyltransferase activity in a polyketide synthase from Toxoplasma gondii"],"prefix":"10.1016","volume":"25","author":[{"given":"Hannah K.","family":"D\u2019Ambrosio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack G.","family":"Ganley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron M.","family":"Keeler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6664-8844","authenticated-orcid":false,"given":"Emily R.","family":"Derbyshire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2022.104443_bib1","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1038\/46829","article-title":"A chain initiation factor common to both modular and aromatic polyketide synthases","volume":"401","author":"Bisang","year":"1999","journal-title":"Nature"},{"key":"10.1016\/j.isci.2022.104443_bib2","doi-asserted-by":"crossref","first-page":"W81","DOI":"10.1093\/nar\/gkz310","article-title":"antiSMASH 5.0: updates to the secondary metabolite genome mining pipeline","volume":"47","author":"Blin","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2022.104443_bib3","first-page":"20201429","article-title":"De novo biosynthesis of simple aromatic compounds by an arthropod (Archegozetes longisetosus)","volume":"287","author":"Br\u00fcckner","year":"2020","journal-title":"Proc. Biol. Sci."},{"key":"10.1016\/j.isci.2022.104443_bib4","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00387-13","article-title":"Evidence for a structural role for acid-fast lipids in oocyst walls of Cryptosporidium, Toxoplasma, and Eimeria","volume":"4","author":"Bushkin","year":"2013","journal-title":"mBio"},{"key":"10.1016\/j.isci.2022.104443_bib5","doi-asserted-by":"crossref","first-page":"11598","DOI":"10.1021\/ja804453p","article-title":"Stereospecificity of ketoreductase domains 1 and 2 of the tylactone modular polyketide synthase","volume":"130","author":"Castonguay","year":"2008","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.isci.2022.104443_bib6","doi-asserted-by":"crossref","first-page":"14349","DOI":"10.1073\/pnas.0603748103","article-title":"Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units","volume":"103","author":"Chan","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U\u00a0S\u00a0A"},{"key":"10.1016\/j.isci.2022.104443_bib7","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.cbpa.2020.06.009","article-title":"Biosynthesis of marine toxins","volume":"59","author":"Chekan","year":"2020","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.isci.2022.104443_bib8","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.chembiol.2007.05.015","article-title":"Structure-based dissociation of a type I polyketide synthase module","volume":"14","author":"Chen","year":"2007","journal-title":"Chem. Biol."},{"key":"10.1016\/j.isci.2022.104443_bib9","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.cell.2017.08.016","article-title":"Genetic mapping and biochemical basis of yellow feather pigmentation in budgerigars","volume":"171","author":"Cooke","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.isci.2022.104443_bib10","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle mesh Ewald: an N\u00b7log(N) method for Ewald sums in large systems","volume":"98","author":"Darden","year":"1993","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.isci.2022.104443_bib11","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1007\/s10295-003-0062-0","article-title":"Active-site residue, domain and module swaps in modular polyketide synthases","volume":"30","author":"Del Vecchio","year":"2003","journal-title":"J.\u00a0Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.isci.2022.104443_bib12","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1021\/bi400185v","article-title":"Mechanism and specificity of an acyltransferase domain from a modular polyketide synthase","volume":"52","author":"Dunn","year":"2013","journal-title":"Biochemistry"},{"key":"10.1016\/j.isci.2022.104443_bib13","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1038\/nature13423","article-title":"Structure of a modular polyketide synthase","volume":"510","author":"Dutta","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.isci.2022.104443_bib14","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1128\/AEM.70.1.104-113.2004","article-title":"Genetics of zwittermicin A production by Bacillus cereus","volume":"70","author":"Emmert","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.isci.2022.104443_bib15","doi-asserted-by":"crossref","first-page":"4912","DOI":"10.1038\/s41467-021-24682-9","article-title":"Mapping the biosynthetic pathway of a hybrid polyketide-nonribosomal peptide in a metazoan","volume":"12","author":"Feng","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2022.104443_bib16","doi-asserted-by":"crossref","first-page":"3468","DOI":"10.1021\/cr0503097","article-title":"Assembly-line enzymology for polyketide and nonribosomal peptide antibiotics: logic machinery, and mechanisms","volume":"37","author":"Fischbach","year":"2006","journal-title":"Chem. Rev."},{"key":"10.1016\/j.isci.2022.104443_bib17","doi-asserted-by":"crossref","first-page":"625","DOI":"10.3390\/molecules25030625","article-title":"Linking genes to molecules in eukaryotic sources: an endeavor to expand our biosynthetic repertoire","volume":"25","author":"Ganley","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.isci.2022.104443_bib18","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1021\/acs.biochem.7b00877","article-title":"Exploring the untapped biosynthetic potential of apicomplexan parasites","volume":"57","author":"Ganley","year":"2017","journal-title":"Biochemistry"},{"key":"10.1016\/j.isci.2022.104443_bib19","doi-asserted-by":"crossref","first-page":"10190","DOI":"10.1021\/ja5056998","article-title":"Elucidation of the cryptic epimerase activity of redox-inactive ketoreductase domains from modular polyketide synthases by tandem equilibrium isotope exchange","volume":"136","author":"Garg","year":"2014","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.isci.2022.104443_bib20","doi-asserted-by":"crossref","first-page":"2524","DOI":"10.1021\/bi971887n","article-title":"Functional orientation of the acyltransferase domain in a module of the erythromycin polyketide synthase","volume":"37","author":"Gokhale","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.isci.2022.104443_bib21","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1016\/S0040-4039(00)87080-X","article-title":"Structure of amipurimycin, a nucleoside antibiotic having a novel branched sugar moiety","volume":"23","author":"Goto","year":"1982","journal-title":"Tetrahedron Lett."},{"key":"10.1016\/j.isci.2022.104443_bib22","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1021\/ct200909j","article-title":"Routine microsecond molecular dynamics simulations with AMBER on GPUs. 1. generalized born","volume":"8","author":"G\u00f6tz","year":"2012","journal-title":"J.\u00a0Chem. Theor. Comput."},{"key":"10.1016\/j.isci.2022.104443_bib23","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1038\/s41429-020-0319-0","article-title":"Identification, cloning and heterologous expression of biosynthetic gene cluster for desertomycin","volume":"73","author":"Hashimoto","year":"2020","journal-title":"J.\u00a0Antibiot."},{"key":"10.1016\/j.isci.2022.104443_bib24","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1038\/nature16993","article-title":"Mycocerosic acid synthase exemplifies the architecture of reducing polyketide synthases","volume":"531","author":"Herbst","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.isci.2022.104443_bib25","doi-asserted-by":"crossref","first-page":"4688","DOI":"10.1002\/anie.200806121","article-title":"The biosynthetic logic of polyketide diversity","volume":"48","author":"Hertweck","year":"2009","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.isci.2022.104443_bib26","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1002\/cbic.201000313","article-title":"Analysis of the sorangicin gene cluster reinforces the utility of a combined phylogenetic\/retrobiosynthetic analysis for deciphering natural product assembly by trans-AT PKS","volume":"11","author":"Irschik","year":"2010","journal-title":"Chembiochem"},{"key":"10.1016\/j.isci.2022.104443_bib27","doi-asserted-by":"crossref","first-page":"6782","DOI":"10.1039\/D1CY00284H","article-title":"Computational studies on the substrate specificity of an acyltransferase domain from salinomycin polyketide synthase","volume":"11","author":"Ji","year":"2021","journal-title":"Catal. Sci. Technol."},{"key":"10.1016\/j.isci.2022.104443_bib28","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.protis.2007.08.001","article-title":"Novel insights into evolution of protistan polyketide synthases through phylogenomic analysis","volume":"159","author":"John","year":"2008","journal-title":"Protist"},{"key":"10.1016\/j.isci.2022.104443_bib29","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.isci.2022.104443_bib30","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1038\/s41467-021-22497-2","article-title":"Computationally-guided exchange of substrate selectivity motifs in a modular polyketide synthase acyltransferase","volume":"12","author":"Kalkreuter","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2022.104443_bib31","first-page":"D454","article-title":"MIBiG 2.0: a repository for biosynthetic gene clusters of known function","volume":"48","author":"Kautsar","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2022.104443_bib32","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1039\/c2np20019h","article-title":"The structures of type I polyketide synthases","volume":"29","author":"Keatinge-Clay","year":"2012","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.isci.2022.104443_bib33","doi-asserted-by":"crossref","first-page":"5334","DOI":"10.1021\/acs.chemrev.6b00683","article-title":"The uncommon enzymology of cis-acyltransferase assembly lines","volume":"117","author":"Keatinge-Clay","year":"2017","journal-title":"Chem. Rev."},{"key":"10.1016\/j.isci.2022.104443_bib34","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0969-2126(03)00004-2","article-title":"Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP Transacylase","volume":"11","author":"Keatinge-Clay","year":"2003","journal-title":"Structure"},{"key":"10.1016\/j.isci.2022.104443_bib35","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1146\/annurev.biochem.76.053105.093515","article-title":"Structure and mechanism of the 6-deoxyerythronolide B synthase","volume":"76","author":"Khosla","year":"2007","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.isci.2022.104443_bib36","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0142731","article-title":"RNA sequencing revealed numerous polyketide synthase genes in the harmful dinoflagellate Karenia mikimotoi","volume":"10","author":"Kimura","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2022.104443_bib37","doi-asserted-by":"crossref","first-page":"e0157901","DOI":"10.1371\/journal.pone.0157901","article-title":"Deciphering the draft genome of Toxoplasma gondii RH strain","volume":"11","author":"Lau","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2022.104443_bib38","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.cpc.2012.09.022","article-title":"SPFP: Speed without compromise - a mixed precision model for GPU accelerated molecular dynamics simulations","volume":"184","author":"Le Grand","year":"2013","journal-title":"Comput. Phys. Commun."},{"key":"10.1016\/j.isci.2022.104443_bib39","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive tree of life (iTOL) v5: an online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2022.104443_bib40","doi-asserted-by":"crossref","first-page":"23203","DOI":"10.1074\/jbc.M112.362210","article-title":"Crystal structure of the acyltransferase domain of the iterative polyketide synthase in enediyne biosynthesis","volume":"287","author":"Liew","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.isci.2022.104443_bib41","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1039\/D1NP00035G","article-title":"Chain release mechanisms in polyketide and non-ribosomal peptide biosynthesis","volume":"39","author":"Little","year":"2021","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.isci.2022.104443_bib42","doi-asserted-by":"crossref","first-page":"7913","DOI":"10.1021\/jacs.7b02875","article-title":"Chemoenzymatic total synthesis and structural diversification of tylactone-based macrolide antibiotics through late-stage polyketide assembly, tailoring, and C-H functionalization","volume":"139","author":"Lowell","year":"2017","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.isci.2022.104443_bib43","doi-asserted-by":"crossref","first-page":"3696","DOI":"10.1021\/acs.jctc.5b00255","article-title":"ff14SB: improving the accuracy of protein side chain and backbone parameters from ff99SB","volume":"11","author":"Maier","year":"2015","journal-title":"J.\u00a0Chem. Theor. Comput."},{"key":"10.1016\/j.isci.2022.104443_bib44","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1093\/nar\/gkr466","article-title":"AntiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences","volume":"39","author":"Medema","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2022.104443_bib45","article-title":"ColabFold - making protein folding accessible to all","author":"Mirdita","year":"2021","journal-title":"BioRxiv"},{"key":"10.1016\/j.isci.2022.104443_bib46","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1038\/s41598-019-38747-9","article-title":"Modular type I polyketide synthase acyl carrier protein domains share a common N-terminally extended fold","volume":"9","author":"Moretto","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2022.104443_bib47","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/0041-0101(85)90031-5","article-title":"The chemistry of brevetoxins: a review","volume":"23","author":"Nakanishi","year":"1985","journal-title":"Toxicon"},{"key":"10.1016\/j.isci.2022.104443_bib48","doi-asserted-by":"crossref","first-page":"12524","DOI":"10.1021\/acs.chemrev.9b00525","article-title":"Evolution and diversity of assembly-line polyketide synthases","volume":"119","author":"Nivina","year":"2019","journal-title":"Chem. Rev."},{"key":"10.1016\/j.isci.2022.104443_bib49","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/j.1469-185X.2008.00040.x","article-title":"Polyketides in insects: ecological role of these widespread chemicals and evolutionary aspects of their biogenesis","volume":"83","author":"Pankewitz","year":"2008","journal-title":"Biol. Rev."},{"key":"10.1016\/j.isci.2022.104443_bib50","doi-asserted-by":"crossref","first-page":"e110965","DOI":"10.1371\/journal.pone.0110965","article-title":"A polyketide synthase acyltransferase domain structure suggests a recognition mechanism for its hydroxymalonyl-acyl carrier protein substrate","volume":"9","author":"Park","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2022.104443_bib51","doi-asserted-by":"crossref","first-page":"4476","DOI":"10.1021\/acs.biochem.6b00639","article-title":"Roles of conserved active site residues in the ketosynthase domain of an assembly line polyketide synthase","volume":"55","author":"Robbins","year":"2016","journal-title":"Biochemistry"},{"key":"10.1016\/j.isci.2022.104443_bib52","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1021\/ct400341p","article-title":"PTRAJ and CPPTRAJ: software for processing and analysis of molecular dynamics trajectory data","volume":"9","author":"Roe","year":"2013","journal-title":"J.\u00a0Chem. Theor. Comput."},{"key":"10.1016\/j.isci.2022.104443_bib53","doi-asserted-by":"crossref","first-page":"14152","DOI":"10.1021\/jacs.9b03021","article-title":"The amipurimycin and miharamycin biosynthetic gene clusters: unraveling the origins of 2-aminopurinyl peptidyl nucleoside antibiotics","volume":"141","author":"Romo","year":"2019","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.isci.2022.104443_bib54","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0021-9991(77)90098-5","article-title":"Numerical integration of the cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes","volume":"23","author":"Ryckaert","year":"1977","journal-title":"J.\u00a0Comput. Phys."},{"key":"10.1016\/j.isci.2022.104443_bib55","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1574-6976.2011.00304.x","article-title":"Microalgae in the postgenomic era: a blooming reservoir for new natural products","volume":"36","author":"Sasso","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.isci.2022.104443_bib56","doi-asserted-by":"crossref","first-page":"3864","DOI":"10.1038\/s41467-021-24133-5","article-title":"Mining and unearthing hidden biosynthetic potential","volume":"12","author":"Scherlach","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2022.104443_bib57","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1038\/nprot.2006.468","article-title":"In-gel digestion for mass spectrometric characterization of proteins and proteomes","volume":"1","author":"Shevchenko","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.isci.2022.104443_bib58","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1038\/nchembio.2144","article-title":"A hybrid polyketide-nonribosomal peptide in nematodes that promotes larval survival","volume":"12","author":"Shou","year":"2016","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.isci.2022.104443_bib59","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.isci.2022.104443_bib60","doi-asserted-by":"crossref","first-page":"2771","DOI":"10.1002\/cctc.202002077","article-title":"Transacylation kinetics in fatty acid and polyketide synthases and its sensitivity to point mutations","volume":"13","author":"Stegemann","year":"2021","journal-title":"ChemCatChem"},{"key":"10.1016\/j.isci.2022.104443_bib61","doi-asserted-by":"crossref","first-page":"2226","DOI":"10.1038\/s41564-019-0539-x","article-title":"Life cycle progression and sexual development of the apicomplexan parasite Cryptosporidium parvum","volume":"4","author":"Tandel","year":"2019","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.isci.2022.104443_bib62","doi-asserted-by":"crossref","first-page":"11124","DOI":"10.1073\/pnas.0601924103","article-title":"The 2.7-\u00c5 crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase","volume":"103","author":"Tang","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U\u00a0S\u00a0A"},{"key":"10.1016\/j.isci.2022.104443_bib63","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/j.chembiol.2007.07.012","article-title":"Structural and mechanistic analysis of protein interactions in module 3 of the 6-deoxyerythronolide B synthase","volume":"14","author":"Tang","year":"2007","journal-title":"Chem. Biol."},{"key":"10.1016\/j.isci.2022.104443_bib64","doi-asserted-by":"crossref","first-page":"2882","DOI":"10.1038\/s41467-020-16376-5","article-title":"Animal biosynthesis of complex polyketides in a photosynthetic partnership","volume":"11","author":"Torres","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2022.104443_bib65","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1039\/C4NP00016A","article-title":"Polyketide biosynthesis in dinoflagellates: what makes it different?","volume":"31","author":"Van Wagoner","year":"2014","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.isci.2022.104443_bib66","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.cell.2019.12.013","article-title":"Identification of a master regulator of differentiation in Toxoplasma","volume":"180","author":"Waldman","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.isci.2022.104443_bib67","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1021\/ar7000414","article-title":"The chemical versatility of natural-product assembly lines","volume":"41","author":"Walsh","year":"2008","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.isci.2022.104443_bib68","doi-asserted-by":"crossref","first-page":"15560","DOI":"10.1002\/anie.201508277","article-title":"Production of the bengamide class of marine natural products in myxobacteria: biosynthesis and structure-activity relationships","volume":"54","author":"Wenzel","year":"2015","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.isci.2022.104443_bib69","doi-asserted-by":"crossref","first-page":"5056","DOI":"10.1021\/bi012086u","article-title":"Quantitative analysis of the relative contributions of donor acyl carrier proteins, acceptor ketosynthases, and linker regions to intermodular transfer of intermediates in hybrid polyketide synthases","volume":"41","author":"Wu","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.isci.2022.104443_bib70","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1021\/np400629v","article-title":"Elucidation of gephyronic acid biosynthetic pathway revealed unexpected sam-dependent methylations","volume":"76","author":"Young","year":"2013","journal-title":"J.\u00a0Nat. Prod."},{"key":"10.1016\/j.isci.2022.104443_bib71","doi-asserted-by":"crossref","first-page":"3708","DOI":"10.1021\/bi300399u","article-title":"Role of a conserved arginine residue in linkers between the ketosynthase and acyltransferase domains of multimodular polyketide synthases","volume":"51","author":"Yuzawa","year":"2012","journal-title":"Biochemistry"},{"key":"10.1016\/j.isci.2022.104443_bib72","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1002\/cbic.201900628","article-title":"Structural and biochemical insight into the recruitment of acyl carrier protein-linked extender units in ansamitocin biosynthesis","volume":"21","author":"Zhang","year":"2020","journal-title":"ChemBioChem"},{"key":"10.1016\/j.isci.2022.104443_bib73","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0378-1119(02)00931-9","article-title":"Cryptosporidium parvum: the first protist known to encode a putative polyketide synthase","volume":"298","author":"Zhu","year":"2002","journal-title":"Gene"},{"key":"10.1016\/j.isci.2022.104443_bib74","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.chembiol.2015.05.010","article-title":"Iterative mechanism of macrodiolide formation in the anticancer compound conglobatin","volume":"22","author":"Zhou","year":"2015","journal-title":"Chem. Biol."},{"key":"10.1016\/j.isci.2022.104443_bib75","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1039\/C8SC03697G","article-title":"Dalmanol biosyntheses require coupling of two separate polyketide gene clusters","volume":"10","author":"Zhou","year":"2019","journal-title":"Chem. Sci."}],"updated-by":[{"DOI":"10.1016\/j.isci.2022.104608","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000}}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004222007143?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004222007143?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T01:22:54Z","timestamp":1742520174000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004222007143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":75,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["S2589004222007143"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2022.104443","relation":{"erratum":[{"id-type":"doi","id":"10.1016\/j.isci.2022.104608","asserted-by":"object"}]},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A single amino acid residue controls acyltransferase activity in a polyketide synthase from Toxoplasma gondii","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2022.104443","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors.","name":"copyright","label":"Copyright"}],"article-number":"104443"}}