{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:49:27Z","timestamp":1772120967665,"version":"3.50.1"},"reference-count":42,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R00AG046911"],"award-info":[{"award-number":["R00AG046911"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AG059099"],"award-info":[{"award-number":["R21AG059099"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2022,11]]},"DOI":"10.1016\/j.isci.2022.105460","type":"journal-article","created":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T05:32:47Z","timestamp":1666935167000},"page":"105460","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["Identifying C.\u00a0elegans lifespan mutants by screening for early-onset protein aggregation"],"prefix":"10.1016","volume":"25","author":[{"given":"Daniel F.","family":"Midkiff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Huayta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James D.","family":"Lichty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph P.","family":"Crapster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0172-2163","authenticated-orcid":false,"given":"Adriana","family":"San-Miguel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2022.105460_bib1","doi-asserted-by":"crossref","first-page":"16010","DOI":"10.1038\/npjamd.2016.10","article-title":"Lifespan-regulating genes in C. elegans","volume":"2","author":"Uno","year":"2016","journal-title":"NPJ Aging Mech. Dis."},{"key":"10.1016\/j.isci.2022.105460_bib2","series-title":"World Population Growth and Aging","author":"Keyfitz","year":"1990"},{"key":"10.1016\/j.isci.2022.105460_bib3","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1038\/366461a0","article-title":"A C. elegans mutant that lives twice as long as wild type","volume":"366","author":"Kenyon","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.isci.2022.105460_bib4","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1101\/gad.1308205","article-title":"A Systematic RNAi Screen for Longevity Genes in C. elegans","volume":"19","author":"Hamilton","year":"2005","journal-title":"Genes. Dev."},{"key":"10.1016\/j.isci.2022.105460_bib5","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/ng1056","article-title":"A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity","volume":"33","author":"Lee","year":"2003","journal-title":"Nat. Genet."},{"key":"10.1016\/j.isci.2022.105460_bib6","doi-asserted-by":"crossref","first-page":"508","DOI":"10.2174\/138920212803251391","article-title":"Genome-wide RNAi longevity screens in Caenorhabditis elegans","volume":"13","author":"Yanos","year":"2012","journal-title":"Curr. Genom."},{"key":"10.1016\/j.isci.2022.105460_bib7","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/0047-6374(83)90082-9","article-title":"A method for the isolation of longevity mutants in the nematode Caenorhabditis elegans and initial results","volume":"22","author":"Klass","year":"1983","journal-title":"Mech. Ageing Dev."},{"key":"10.1016\/j.isci.2022.105460_bib8","doi-asserted-by":"crossref","first-page":"B102","DOI":"10.1093\/geronj\/43.4.B102","article-title":"Three mutants that extend both mean and maximum life span of the nematode, Caenorhabditis elegans, define the age-1 gene","volume":"43","author":"Friedman","year":"1988","journal-title":"J.\u00a0Gerontol."},{"key":"10.1016\/j.isci.2022.105460_bib9","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1038\/s41467-021-21743-x","article-title":"Mutagenesis screen uncovers lifespan extension through integrated stress response inhibition without reduced mRNA translation","volume":"12","author":"Derisbourg","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2022.105460_bib10","first-page":"387","article-title":"A Transparent window into biology: a primer on Caenorhabditis elegans","volume":"200","author":"Corsi","year":"2015","journal-title":"WormBook"},{"key":"10.1016\/j.isci.2022.105460_bib11","unstructured":"AhringerJ. (ed.), Reverse genetics. WormBook. 10.1895\/wormbook.1.47.1"},{"key":"10.1016\/j.isci.2022.105460_bib12","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1371\/journal.pgen.0010017","article-title":"New genes tied to endocrine, metabolic, and dietary regulation of lifespan from a Caenorhabditis elegans genomic RNAi screen","volume":"1","author":"Hansen","year":"2005","journal-title":"PLoS Genet."},{"key":"10.1016\/j.isci.2022.105460_bib13","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1038\/nrg794","article-title":"The art and design of genetic screens: Caenorhabditis elegans","volume":"3","author":"Jorgensen","year":"2002","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.isci.2022.105460_bib14","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1038\/nprot.2006.245","article-title":"RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions","volume":"1","author":"Lehner","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.isci.2022.105460_bib15","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1534\/g3.119.400241","article-title":"Forward genetic screen for Caenorhabditis elegans mutants with a shortened locomotor healthspan","volume":"9","author":"Kawamura","year":"2019","journal-title":"G3"},{"key":"10.1016\/j.isci.2022.105460_bib16","doi-asserted-by":"crossref","first-page":"18927","DOI":"10.1073\/pnas.1013854107","article-title":"3-Ketoacyl thiolase delays aging of Caenorhabditis elegans and is required for lifespan extension mediated by sir-2.1","volume":"107","author":"Berdichevsky","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2022.105460_bib17","doi-asserted-by":"crossref","first-page":"889","DOI":"10.18632\/aging.100936","article-title":"Autofluorescence as a measure of senescence in C. elegans: look to red, not blue or green","volume":"8","author":"Pincus","year":"2016","journal-title":"Aging"},{"key":"10.1016\/j.isci.2022.105460_bib18","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.mad.2016.01.004","article-title":"C.\u00a0elegans lifespan extension by osmotic stress requires FUdR, base excision repair, FOXO, and sirtuins","volume":"154","author":"Anderson","year":"2016","journal-title":"Mech. Ageing Dev."},{"key":"10.1016\/j.isci.2022.105460_bib19","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.mad.2011.08.006","article-title":"FUdR causes a twofold increase in the lifespan of the mitochondrial mutant gas-1","volume":"132","author":"Van Raamsdonk","year":"2011","journal-title":"Mech. Ageing Dev."},{"key":"10.1016\/j.isci.2022.105460_bib20","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.mad.2010.03.002","article-title":"The use of FUdR can cause prolonged longevity in mutant nematodes","volume":"131","author":"Aitlhadj","year":"2010","journal-title":"Mech. Ageing Dev."},{"key":"10.1016\/j.isci.2022.105460_bib21","doi-asserted-by":"crossref","first-page":"e1000450","DOI":"10.1371\/journal.pbio.1000450","article-title":"Widespread protein aggregation as an inherent part of aging in C. elegans","volume":"8","author":"David","year":"2010","journal-title":"PLoS Biol."},{"key":"10.1016\/j.isci.2022.105460_bib22","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.cell.2015.03.032","article-title":"Widespread proteome remodeling and aggregation in aging C. elegans","volume":"161","author":"Walther","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.isci.2022.105460_bib23","doi-asserted-by":"crossref","first-page":"13023","DOI":"10.1038\/ncomms13023","article-title":"Large-scale microfluidics providing high-resolution and high-throughput screening of Caenorhabditis elegans poly-glutamine aggregation model","volume":"7","author":"Mondal","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2022.105460_bib24","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.cell.2010.07.020","article-title":"Identification of MOAG-4\/SERF as a regulator of age-related proteotoxicity","volume":"142","author":"van Ham","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.isci.2022.105460_bib25","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.celrep.2016.12.033","article-title":"Reduced insulin\/IGF-1 signaling restores the dynamic properties of key stress granule proteins during aging","volume":"18","author":"Lechler","year":"2017","journal-title":"Cell Rep."},{"key":"10.1016\/j.isci.2022.105460_bib26","doi-asserted-by":"crossref","first-page":"3090","DOI":"10.1039\/C8LC00655E","article-title":"A microfluidic platform for lifelong high-resolution and high throughput imaging of subtle aging phenotypes in C. elegans","volume":"18","author":"Saberi-Bosari","year":"2018","journal-title":"Lab. Chip"},{"key":"10.1016\/j.isci.2022.105460_bib27","doi-asserted-by":"crossref","first-page":"12990","DOI":"10.1038\/ncomms12990","article-title":"Deep phenotyping unveils hidden traits and genetic relations in subtle mutants","volume":"7","author":"San-Miguel","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2022.105460_bib28","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1080\/15548627.2018.1445914","article-title":"Age-associated and tissue-specific decline in autophagic activity in the nematode C. elegans","volume":"14","author":"Chang","year":"2018","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2022.105460_bib29","doi-asserted-by":"crossref","first-page":"e109633","DOI":"10.15252\/embj.2021109633","article-title":"Ageing induces tissue-specific transcriptomic changes in Caenorhabditis elegans","volume":"41","author":"Wang","year":"2022","journal-title":"EMBO J."},{"key":"10.1016\/j.isci.2022.105460_bib30","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.exger.2005.12.004","article-title":"Sarcopenia in the Caenorhabditis elegans pharynx correlates with muscle contraction rate over lifespan","volume":"41","author":"Chow","year":"2006","journal-title":"Exp. Gerontol."},{"key":"10.1016\/j.isci.2022.105460_bib31","doi-asserted-by":"crossref","first-page":"R397","DOI":"10.1016\/j.cub.2009.03.013","article-title":"Stress granules","volume":"19","author":"Anderson","year":"2009","journal-title":"Curr. Biol."},{"key":"10.1016\/j.isci.2022.105460_bib32","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1534\/g3.117.300135","article-title":"Use of a sibling subtraction method for identifying causal mutations in Caenorhabditis elegans by whole-genome sequencing","volume":"8","author":"Joseph","year":"2018","journal-title":"G3"},{"key":"10.1016\/j.isci.2022.105460_bib33","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1091\/mbc.e03-07-0532","article-title":"Regulation of longevity in Caenorhabditis elegans by heat shock factor and molecular chaperones","volume":"15","author":"Morley","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.isci.2022.105460_bib34","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/j.bbrc.2018.12.161","article-title":"Age-dependent effects of floxuridine (FUdR) on senescent pathology and mortality in the nematode Caenorhabditis elegans","volume":"509","author":"Wang","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.isci.2022.105460_bib35","doi-asserted-by":"crossref","first-page":"733","DOI":"10.18632\/aging.100367","article-title":"Longevity and stress in Caenorhabditis elegans","volume":"3","author":"Zhou","year":"2011","journal-title":"Aging"},{"key":"10.1016\/j.isci.2022.105460_bib36","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/j.exger.2006.09.004","article-title":"Hormesis and aging in Caenorhabditis elegans","volume":"41","author":"Cypser","year":"2006","journal-title":"Exp. Gerontol."},{"key":"10.1016\/j.isci.2022.105460_bib37","first-page":"51","article-title":"Maintenance of C. elegans","volume":"2","author":"Stiernagle","year":"2006","journal-title":"WormBook"},{"key":"10.1016\/j.isci.2022.105460_bib38","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1038\/nmeth.2141","article-title":"Autonomous screening of C. elegans identifies genes implicated in\u00a0synaptogenesis","volume":"9","author":"Crane","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.isci.2022.105460_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1895\/wormbook.1.167.1","article-title":"Forward and reverse mutagenesis in C. elegans","author":"Kutscher","year":"2014","journal-title":"WormBook"},{"key":"10.1016\/j.isci.2022.105460_bib40","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1016\/j.cub.2021.03.069","article-title":"BBLN-1 is essential for intermediate filament organization and apical membrane morphology","volume":"31","author":"Remmelzwaal","year":"2021","journal-title":"Curr. Biol."},{"key":"10.1016\/j.isci.2022.105460_bib41","doi-asserted-by":"crossref","first-page":"56147","DOI":"10.18632\/oncotarget.11269","article-title":"OASIS 2: online application for survival analysis 2 with features for the analysis of maximal lifespan and healthspan in aging research","volume":"7","author":"Han","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.isci.2022.105460_bib42","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004222017321?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004222017321?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T17:09:25Z","timestamp":1762880965000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004222017321"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":42,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["S2589004222017321"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2022.105460","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.12.14.472506","asserted-by":"object"}]},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identifying C.\u00a0elegans lifespan mutants by screening for early-onset protein aggregation","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2022.105460","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Author(s).","name":"copyright","label":"Copyright"}],"article-number":"105460"}}