{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T21:22:29Z","timestamp":1793740949549,"version":"4.6.1"},"reference-count":49,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T00:00:00Z","timestamp":1693267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2023,10]]},"DOI":"10.1016\/j.isci.2023.107804","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T21:33:58Z","timestamp":1693431238000},"page":"107804","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["GATA1 deletion in human pluripotent stem cells increases differentiation yield and maturity of neutrophils"],"prefix":"10.1016","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8844-8445","authenticated-orcid":false,"given":"Thomas C.","family":"Harper","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2809-0506","authenticated-orcid":false,"given":"Elaine M.","family":"Oberlick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1439-7462","authenticated-orcid":false,"given":"Tomas J.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan E.","family":"Nunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark-Anthony","family":"Bray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seonmi","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey D.","family":"Driscoll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah F.","family":"Mowbray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Antczak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2023.107804_bib1","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1126\/science.1092385","article-title":"Neutrophil Extracellular Traps Kill Bacteria","volume":"303","author":"Brinkmann","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.isci.2023.107804_bib2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1146\/annurev.immunol.23.021704.115653","article-title":"How neutrophils kill microbes","volume":"23","author":"Segal","year":"2005","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.isci.2023.107804_bib3","doi-asserted-by":"crossref","first-page":"178ra40","DOI":"10.1126\/scitranslmed.3005580","article-title":"NETs are a source of citrullinated autoantigens and stimulate inflammatory responses in rheumatoid arthritis","volume":"5","author":"Khandpur","year":"2013","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.isci.2023.107804_bib4","doi-asserted-by":"crossref","first-page":"928","DOI":"10.3389\/fimmu.2017.00928","article-title":"Neutrophil Extracellular Traps and endothelial dysfunction in atherosclerosis and thrombosis","volume":"8","author":"Qi","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2023.107804_bib5","doi-asserted-by":"crossref","first-page":"31119","DOI":"10.1038\/srep31119","article-title":"Neutrophil extracellular trap formation is increased in psoriasis and induces human \u03b2-defensin-2 production in epidermal keratinocytes","volume":"6","author":"Hu","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2023.107804_bib6","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s12931-015-0221-7","article-title":"Neutrophil extracellular trap (NET) formation characterises stable and exacerbated COPD and correlates with airflow limitation","volume":"16","author":"Grabcanovic-Musija","year":"2015","journal-title":"Respir. Res."},{"key":"10.1016\/j.isci.2023.107804_bib7","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.immuni.2019.07.002","article-title":"Neutrophil Extracellular Traps Initiate Gallstone Formation","volume":"51","author":"Mu\u00f1oz","year":"2019","journal-title":"Immunity"},{"key":"10.1016\/j.isci.2023.107804_bib8","doi-asserted-by":"crossref","first-page":"19630","DOI":"10.1038\/s41598-020-76781-0","article-title":"The emerging role of neutrophil extracellular traps in severe acute respiratory syndrome coronavirus 2 (COVID-19)","volume":"10","author":"Arcanjo","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2023.107804_bib9","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20201129","article-title":"SARS-CoV-2-triggered neutrophil extracellular traps mediate COVID-19 pathology","volume":"217","author":"Veras","year":"2020","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.isci.2023.107804_bib10","doi-asserted-by":"crossref","first-page":"118617","DOI":"10.1016\/j.lfs.2020.118617","article-title":"Preventing the development of severe COVID-19 by modifying immunothrombosis","volume":"264","author":"Morris","year":"2021","journal-title":"Life Sci."},{"key":"10.1016\/j.isci.2023.107804_bib11","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0140-6736(20)30183-5","article-title":"Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China","volume":"395","author":"Huang","year":"2020","journal-title":"Lancet"},{"key":"10.1016\/j.isci.2023.107804_bib12","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1007\/s10735-020-09915-3","article-title":"COVID-19 and multiorgan failure: A narrative review on potential mechanisms","volume":"51","author":"Mokhtari","year":"2020","journal-title":"J.\u00a0Mol. Histol."},{"key":"10.1016\/j.isci.2023.107804_bib13","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1038\/s41577-020-0407-1","article-title":"Tissue damage from neutrophil-induced oxidative stress in COVID-19","volume":"20","author":"Laforge","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.isci.2023.107804_bib14","article-title":"Neutrophil extracellular traps in COVID-19","volume":"5","author":"Zuo","year":"2020","journal-title":"JCI Insight"},{"key":"10.1016\/j.isci.2023.107804_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/cells10010151","article-title":"Significance of nets formation in COVID-19","volume":"10","author":"Janiuk","year":"2021","journal-title":"Cells"},{"key":"10.1016\/j.isci.2023.107804_bib16","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.157342","article-title":"Disulfiram inhibits neutrophil extracellular trap formation and protects rodents from acute lung injury and SARS-CoV-2 infection","volume":"7","author":"Adrover","year":"2022","journal-title":"JCI Insight"},{"key":"10.1016\/j.isci.2023.107804_bib17","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/s41591-021-01576-3","article-title":"Dexamethasone modulates immature neutrophils and interferon programming in severe COVID-19","volume":"28","author":"Sinha","year":"2022","journal-title":"Nat. Med."},{"key":"10.1016\/j.isci.2023.107804_bib18","doi-asserted-by":"crossref","first-page":"141","DOI":"10.2147\/JIR.S284941","article-title":"Studying neutrophil function in\u00a0vitro: Cell models and environmental factors","volume":"14","author":"Blanter","year":"2021","journal-title":"J.\u00a0Inflamm. Res."},{"key":"10.1016\/j.isci.2023.107804_bib19","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1177\/1087057115588287","article-title":"High-throughput screening using iPSC-derived neuronal progenitors to identify compounds counteracting epigenetic gene silencing in fragile X syndrome","volume":"20","author":"Kaufmann","year":"2015","journal-title":"J.\u00a0Biomol. Screen"},{"key":"10.1016\/j.isci.2023.107804_bib20","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1053\/j.gastro.2020.10.002","article-title":"High-Fidelity Drug-Induced Liver Injury Screen Using Human Pluripotent Stem Cell-Derived Organoids","volume":"160","author":"Shinozawa","year":"2021","journal-title":"Gastroenterology"},{"key":"10.1016\/j.isci.2023.107804_bib21","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/sctm.18-0255","article-title":"Neutrophils Derived from Genetically Modified Human Induced Pluripotent Stem Cells Circulate and Phagocytose Bacteria In\u00a0Vivo","volume":"8","author":"Trump","year":"2019","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.isci.2023.107804_bib22","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/j.stemcr.2019.10.007","article-title":"Effective and Rapid Generation of Functional Neutrophils from Induced Pluripotent Stem Cells Using ETV2-Modified mRNA","volume":"13","author":"Brok-Volchanskaya","year":"2019","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.isci.2023.107804_bib23","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.stemcr.2015.01.005","article-title":"Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies","volume":"4","author":"Lachmann","year":"2015","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.isci.2023.107804_bib24","doi-asserted-by":"crossref","first-page":"111128","DOI":"10.1016\/j.celrep.2022.111128","article-title":"Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy","volume":"40","author":"Chang","year":"2022","journal-title":"Cell Rep."},{"key":"10.1016\/j.isci.2023.107804_bib25","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1182\/blood-2021-150495","article-title":"Induced Pluripotent Stem Cell-Derived Neutrophil Granulocytes - Flow Cytometry Analysis Reveals Distinct Subpopulations","volume":"138","author":"Frenz","year":"2021","journal-title":"Blood"},{"key":"10.1016\/j.isci.2023.107804_bib26","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1182\/blood-2013-12-543850","article-title":"Acetylation of C\/EBP\u03b5 is a prerequisite for terminal neutrophil differentiation","volume":"125","author":"Bartels","year":"2015","journal-title":"Blood"},{"key":"10.1016\/j.isci.2023.107804_bib27","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1016\/j.cell.2015.11.013","article-title":"Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors","volume":"163","author":"Paul","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.isci.2023.107804_bib28","doi-asserted-by":"crossref","first-page":"4322","DOI":"10.1182\/blood-2002-03-0835","article-title":"The in\u00a0vivo profile of transcription factors during neutrophil differentiation in human bone marrow","volume":"101","author":"Bjerregaard","year":"2003","journal-title":"Blood"},{"key":"10.1016\/j.isci.2023.107804_bib29","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1084\/jem.20020170","article-title":"Essential and instructive roles of GATA factors in eosinophil development","volume":"195","author":"Hirasawa","year":"2002","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.isci.2023.107804_bib30","doi-asserted-by":"crossref","first-page":"18620","DOI":"10.1073\/pnas.1311668110","article-title":"GATA-1 regulates the generation and function of basophils","volume":"110","author":"Nei","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"10.1016\/j.isci.2023.107804_bib31","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1016\/j.stemcr.2022.10.007","article-title":"Notch activation during early mesoderm induction modulates emergence of the T\/NK cell lineage from human iPSCs","volume":"17","author":"Heinze","year":"2022","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.isci.2023.107804_bib32","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/ncb3161","article-title":"Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages","volume":"17","author":"Ditadi","year":"2015","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.isci.2023.107804_bib33","doi-asserted-by":"crossref","first-page":"2791","DOI":"10.1182\/blood.V78.11.2791.bloodjournal78112791","article-title":"Granulocyte colony-stimulating factor and its receptor","volume":"78","author":"Demetri","year":"1991","journal-title":"Blood"},{"key":"10.1016\/j.isci.2023.107804_bib34","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1182\/blood-2006-09-048322","article-title":"Gata1 regulates dendritic-cell development and survival","volume":"110","author":"Guti\u00e9rrez","year":"2007","journal-title":"Blood"},{"key":"10.1016\/j.isci.2023.107804_bib35","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.1182\/blood-2012-05-432088","article-title":"Eosinophil extracellular DNA trap cell death mediates lytic release of free secretion-competent eosinophil granules in humans","volume":"121","author":"Ueki","year":"2013","journal-title":"Blood"},{"key":"10.1016\/j.isci.2023.107804_bib36","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1111\/imm.12790","article-title":"Neither eosinophils nor neutrophils require ATG5-dependent autophagy for extracellular DNA trap formation","volume":"152","author":"Germic","year":"2017","journal-title":"Immunology"},{"key":"10.1016\/j.isci.2023.107804_bib37","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.24437","article-title":"Diverse stimuli engage different neutrophil extracellular trap pathways","volume":"6","author":"Kenny","year":"2017","journal-title":"Elife"},{"key":"10.1016\/j.isci.2023.107804_bib38","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1038\/s41590-020-0669-6","article-title":"FDA-approved disulfiram inhibits pyroptosis by blocking gasdermin D pore formation","volume":"21","author":"Hu","year":"2020","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.isci.2023.107804_bib39","doi-asserted-by":"crossref","first-page":"6689","DOI":"10.1126\/sciimmunol.aar6689","article-title":"Gasdermin D plays a vital role in the generation of neutrophil extracellular traps","volume":"3","author":"Sollberger","year":"2018","journal-title":"Sci. Immunol."},{"key":"10.1016\/j.isci.2023.107804_bib40","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1182\/blood-2010-06-290171","article-title":"Myeloperoxidase is required for neutrophil extracellular trap formation: Implications for innate immunity","volume":"117","author":"Metzler","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.isci.2023.107804_bib41","doi-asserted-by":"crossref","first-page":"3409","DOI":"10.1038\/s41598-017-03257-z","article-title":"JNK Activation Turns on LPS-And Gram-Negative Bacteria-Induced NADPH Oxidase-Dependent Suicidal NETosis","volume":"7","author":"Khan","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2023.107804_bib42","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1038\/nm.1855","article-title":"Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense","volume":"14","author":"Yousefi","year":"2008","journal-title":"Nat. Med."},{"key":"10.1016\/j.isci.2023.107804_bib43","doi-asserted-by":"crossref","first-page":"8061","DOI":"10.1038\/s41598-019-44627-z","article-title":"Eosinophil extracellular trap formation is closely associated with disease severity in chronic rhinosinusitis regardless of nasal polyp status","volume":"9","author":"Hwang","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2023.107804_bib44","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1083\/jcb.201006052","article-title":"Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps","volume":"191","author":"Papayannopoulos","year":"2010","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.isci.2023.107804_bib45","doi-asserted-by":"crossref","first-page":"17624","DOI":"10.1074\/jbc.RA120.015682","article-title":"NETosis occurs independently of neutrophil serine proteases","volume":"295","author":"Kasperkiewicz","year":"2020","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.isci.2023.107804_bib46","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1111\/jth.13239","article-title":"Neutrophil elastase-deficient mice form neutrophil extracellular traps in an experimental model of deep vein thrombosis","volume":"14","author":"Martinod","year":"2016","journal-title":"J.\u00a0Thromb. Haemost."},{"key":"10.1016\/j.isci.2023.107804_bib47","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1365-2249.2008.03737.x","article-title":"Prognostic value of phagocytic activity of neutrophils and monocytes in sepsis. Correlation to CD64 and CD14 antigen expression","volume":"154","author":"Danikas","year":"2008","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.isci.2023.107804_bib48","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1172\/JCI105568","article-title":"In\u00a0Vitro Bactericidal Capacity of Human Polymorphonuclear Leukocytes: Diminished Activity in Chronic Granulomatous Disease of Childhood","volume":"46","author":"Quie","year":"1967","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.isci.2023.107804_bib49","article-title":"High-Throughput Real-Time Quantitative Reverse Transcription PCR","volume":"Chapter 15","author":"Bookout","year":"2006","journal-title":"Curr. Protoc. Mol. Biol."}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004223018813?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004223018813?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T14:56:22Z","timestamp":1754405782000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004223018813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10]]},"references-count":49,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["S2589004223018813"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2023.107804","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2023,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"GATA1 deletion in human pluripotent stem cells increases differentiation yield and maturity of neutrophils","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2023.107804","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors.","name":"copyright","label":"Copyright"}],"article-number":"107804"}}