{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:31:34Z","timestamp":1790713894208,"version":"4.1.0"},"reference-count":81,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011280","name":"University of South Florida Morsani College of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011280","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1016\/j.isci.2024.109602","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T04:58:55Z","timestamp":1711515535000},"page":"109602","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transforming the CRISPR\/dCas9-based gene regulation technique into a forward screening tool in Plasmodium falciparum"],"prefix":"10.1016","volume":"27","author":[{"given":"Amuza Byaruhanga","family":"Lucky","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azhar","family":"Muneer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9374-6749","authenticated-orcid":false,"given":"Jun","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2024.109602_bib1","series-title":"Would Malaria Report 2023. Geneva","year":"2023"},{"key":"10.1016\/j.isci.2024.109602_bib2","series-title":"Full Evidence Report on the RTS,S\/AS01 Malaria Vaccine","year":"2021"},{"key":"10.1016\/j.isci.2024.109602_bib3","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1056\/NEJMc2117480","article-title":"Evidence of Artemisinin-Resistant Malaria in Africa","volume":"386","author":"Stokes","year":"2022","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.isci.2024.109602_bib4","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1056\/NEJMoa1314981","article-title":"Spread of artemisinin resistance in Plasmodium falciparum malaria","volume":"371","author":"Ashley","year":"2014","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.isci.2024.109602_bib5","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1097\/QCO.0000000000000766","article-title":"Artemisinin and multidrug-resistant Plasmodium falciparum - a threat for malaria control and elimination","volume":"34","author":"Dhorda","year":"2021","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.isci.2024.109602_bib6","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1016\/S1473-3099(19)30391-3","article-title":"Determinants of dihydroartemisinin-piperaquine treatment failure in Plasmodium falciparum malaria in Cambodia, Thailand, and Vietnam: a prospective clinical, pharmacological, and genetic study","volume":"19","author":"van der Pluijm","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.isci.2024.109602_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1001138","article-title":"Chemical rescue of malaria parasites lacking an apicoplast defines organelle function in blood-stage Plasmodium falciparum","volume":"9","author":"Yeh","year":"2011","journal-title":"PLoS Biol."},{"key":"10.1016\/j.isci.2024.109602_bib8","doi-asserted-by":"crossref","DOI":"10.1126\/science.aap7847","article-title":"Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis","volume":"360","author":"Zhang","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.isci.2024.109602_bib9","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1126\/science.aac7041","article-title":"Identification and characterization of essential genes in the human genome","volume":"350","author":"Wang","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.isci.2024.109602_bib10","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nbt.2800","article-title":"Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library","volume":"32","author":"Koike-Yusa","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.isci.2024.109602_bib11","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1247005","article-title":"Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells","volume":"343","author":"Shalem","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.isci.2024.109602_bib12","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1016\/j.cell.2016.05.003","article-title":"A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria","volume":"165","author":"Peters","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.isci.2024.109602_bib13","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.cell.2014.09.029","article-title":"Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation","volume":"159","author":"Gilbert","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.isci.2024.109602_bib14","doi-asserted-by":"crossref","DOI":"10.1038\/s43586-021-00093-4","article-title":"High-content CRISPR screening","volume":"2","author":"Bock","year":"2022","journal-title":"Nat. Rev. Methods Primers"},{"key":"10.1016\/j.isci.2024.109602_bib15","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241914865","article-title":"CRISPR\/dCas9 Tools: Epigenetic Mechanism and Application in Gene Transcriptional Regulation","volume":"24","author":"Cai","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.isci.2024.109602_bib16","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.cell.2016.08.019","article-title":"A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes","volume":"166","author":"Sidik","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.isci.2024.109602_bib17","doi-asserted-by":"crossref","first-page":"3963","DOI":"10.1038\/s41467-019-11855-w","article-title":"A CRISPR platform for targeted in\u00a0vivo screens identifies Toxoplasma gondii virulence factors in mice","volume":"10","author":"Young","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2024.109602_bib18","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.pt.2019.03.004","article-title":"CRISPR in Parasitology: Not Exactly Cut and Dried","volume":"35","author":"Bryant","year":"2019","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.isci.2024.109602_bib19","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.chom.2019.09.008","article-title":"In\u00a0Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration","volume":"26","author":"Sangar\u00e9","year":"2019","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.isci.2024.109602_bib20","article-title":"Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFN gamma-activated murine macrophages","volume":"11","author":"Wang","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2024.109602_bib21","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.cell.2019.12.013","article-title":"Identification of a Master Regulator of Differentiation in Toxoplasma","volume":"180","author":"Waldman","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.isci.2024.109602_bib22","doi-asserted-by":"crossref","first-page":"4813","DOI":"10.1038\/s41467-020-18624-0","article-title":"Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility","volume":"11","author":"Harding","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2024.109602_bib23","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1038\/s41564-022-01114-y","article-title":"A splitCas9 phenotypic screen in Toxoplasma gondii identifies proteins involved in host cell egress and invasion","volume":"7","author":"Li","year":"2022","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.isci.2024.109602_bib24","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1038\/s41564-022-01104-0","article-title":"Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress","volume":"7","author":"Smith","year":"2022","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.isci.2024.109602_bib25","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.85605","article-title":"Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR\/Cas9 cytosine base editor toolbox LeishBASEedit","volume":"12","author":"Engstler","year":"2023","journal-title":"Elife"},{"key":"10.1016\/j.isci.2024.109602_bib26","doi-asserted-by":"crossref","first-page":"9555","DOI":"10.1074\/jbc.RA118.007121","article-title":"Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectious Plasmodium yoelii","volume":"294","author":"Walker","year":"2019","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.isci.2024.109602_bib27","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.cell.2017.06.030","article-title":"Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes","volume":"170","author":"Bushell","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.isci.2024.109602_bib28","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.pt.2020.11.007","article-title":"Essential Genes of the Parasitic Apicomplexa","volume":"37","author":"Oberstaller","year":"2021","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.isci.2024.109602_bib29","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cels.2022.12.001","article-title":"Gene-by-gene screen of the unknown proteins encoded on Plasmodium falciparum chromosome 3","volume":"14","author":"Kimmel","year":"2023","journal-title":"Cell Syst."},{"key":"10.1016\/j.isci.2024.109602_bib30","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.ijpara.2019.04.003","article-title":"Generation and functional characterisation of Plasmodium yoelii csp deletion mutants using a microhomology-based CRISPR\/Cas9 method","volume":"49","author":"Xu","year":"2019","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.isci.2024.109602_bib31","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.02782-21","article-title":"A Leak-Free Inducible CRISPRi\/a System for Gene Functional Studies in Plasmodium falciparum","volume":"10","author":"Liang","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.isci.2024.109602_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-020-15026-0","article-title":"Dissecting the role of PfAP2-G in malaria gametocytogenesis","volume":"11","author":"Josling","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2024.109602_bib33","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nature12920","article-title":"A transcriptional switch underlies commitment to sexual development in malaria parasites","volume":"507","author":"Kafsack","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.isci.2024.109602_bib34","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02558-21","article-title":"Malaria Parasite Stress Tolerance Is Regulated by DNMT2-Mediated tRNA Cytosine Methylation","volume":"12","author":"Hammam","year":"2021","journal-title":"mBio"},{"key":"10.1016\/j.isci.2024.109602_bib35","doi-asserted-by":"crossref","first-page":"e0110122","DOI":"10.1128\/spectrum.01101-22","article-title":"The Knock-Down of the Chloroquine Resistance Transporter PfCRT Is Linked to Oligopeptide Handling in","volume":"10","author":"Sanchez","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.isci.2024.109602_bib36","doi-asserted-by":"crossref","first-page":"3356","DOI":"10.1073\/pnas.1417102112","article-title":"chloroquine resistance transporter is a H+-coupled polyspecific nutrient and drug exporter","volume":"112","author":"Juge","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2024.109602_bib37","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/nbt.3853","article-title":"CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome","volume":"35","author":"Klann","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.isci.2024.109602_bib38","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jcf.2021.04.008","article-title":"Epigenome editing of the CFTR-locus for treatment of cystic fibrosis","volume":"21","author":"Kabadi","year":"2022","journal-title":"J.\u00a0Cyst. Fibros."},{"key":"10.1016\/j.isci.2024.109602_bib39","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.molcel.2017.09.012","article-title":"Combined CRISPRi\/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent","volume":"68","author":"Jost","year":"2017","journal-title":"Mol. Cell"},{"key":"10.1016\/j.isci.2024.109602_bib40","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1186\/s12936-019-2980-y","article-title":"Uptake of a fluorescently tagged chloroquine analogue is reduced in CQ-resistant compared to CQ-sensitive Plasmodium falciparum parasites","volume":"18","author":"Reiling","year":"2019","journal-title":"Malar. J."},{"key":"10.1016\/j.isci.2024.109602_bib41","doi-asserted-by":"crossref","first-page":"7891","DOI":"10.1128\/AAC.01139-15","article-title":"3-Halo Chloroquine Derivatives Overcome Plasmodium falciparum Chloroquine Resistance Transporter-Mediated Drug Resistance in P. falciparum","volume":"59","author":"Edaye","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.isci.2024.109602_bib42","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.1015957","article-title":"In\u00a0vitro susceptibility profile of Plasmodium falciparum clinical isolates from Ghana to antimalarial drugs and polymorphisms in resistance markers","volume":"12","author":"Zhao","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.isci.2024.109602_bib43","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1126\/science.1074045","article-title":"Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations","volume":"298","author":"Sidhu","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.isci.2024.109602_bib44","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41598-020-79539-w","article-title":"Histone acetyltransferase PfGCN5 regulates stress responsive and artemisinin resistance related genes in Plasmodium falciparum","volume":"11","author":"Rawat","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2024.109602_bib45","article-title":"Role of PfGCN5 in nutrient sensing and transcriptional regulation in Plasmodium falciparum","volume":"45","author":"Rawat","year":"2020","journal-title":"J.\u00a0Bio. Sci."},{"key":"10.1016\/j.isci.2024.109602_bib46","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1038\/s41467-020-20805-w","article-title":"Artemisinin-resistant K13 mutations rewire Plasmodium falciparum's intra-erythrocytic metabolic program to enhance survival","volume":"12","author":"Mok","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2024.109602_bib47","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1126\/science.1260403","article-title":"Population transcriptomics of human malaria parasites reveals the mechanism of artemisinin resistance","volume":"347","author":"Mok","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.isci.2024.109602_bib48","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-018-06221-1","article-title":"Artemisinin kills malaria parasites by damaging proteins and inhibiting the proteasome","volume":"9","author":"Bridgford","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2024.109602_bib49","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1006930","article-title":"Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites","volume":"14","author":"Rocamora","year":"2018","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.isci.2024.109602_bib50","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1365-2958.2012.08180.x","article-title":"Mechanisms of in\u00a0vitro resistance to dihydroartemisinin in Plasmodium falciparum","volume":"86","author":"Cui","year":"2012","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.isci.2024.109602_bib51","doi-asserted-by":"crossref","DOI":"10.1128\/aac.00577-23","article-title":"Plasmodium falciparum GCN5 plays a key role in regulating artemisinin resistance-related stress responses","volume":"67","author":"Lucky","year":"2023","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.isci.2024.109602_bib52","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1111\/j.1365-2958.2010.07371.x","article-title":"The MYST family histone acetyltransferase regulates gene expression and cell cycle in malaria parasite Plasmodium falciparum","volume":"78","author":"Miao","year":"2010","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.isci.2024.109602_bib53","doi-asserted-by":"crossref","first-page":"622","DOI":"10.4103\/ijmr.IJMR_1688_15","article-title":"In\u00a0vitro susceptibility of Indian Plasmodium falciparum isolates to different antimalarial drugs\u00a0& antibiotics","volume":"146","author":"Agarwal","year":"2017","journal-title":"Indian J. Med. Res."},{"key":"10.1016\/j.isci.2024.109602_bib54","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/nmeth.4223","article-title":"A genetic system to study Plasmodium falciparum protein function","volume":"14","author":"Birnbaum","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.isci.2024.109602_bib55","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/ng.3189","article-title":"Genetic architecture of artemisinin-resistant Plasmodium falciparum","volume":"47","author":"Miotto","year":"2015","journal-title":"Nat. Genet."},{"key":"10.1016\/j.isci.2024.109602_bib56","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.pt.2006.06.014","article-title":"Hypoglycemia in falciparum malaria: is fasting an unrecognized and insufficiently emphasized risk factor?","volume":"22","author":"Thien","year":"2006","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.isci.2024.109602_bib57","doi-asserted-by":"crossref","first-page":"e971","DOI":"10.1038\/cddis.2013.505","article-title":"Turning up the heat: heat stress induces markers of programmed cell death in Plasmodium falciparum in\u00a0vitro","volume":"4","author":"Engelbrecht","year":"2013","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.isci.2024.109602_bib58","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1128\/IAI.01236-06","article-title":"Molecular factors and biochemical pathways induced by febrile temperature in intraerythrocytic Plasmodium falciparum parasites","volume":"75","author":"Oakley","year":"2007","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.109602_bib59","doi-asserted-by":"crossref","first-page":"4563","DOI":"10.1038\/s41467-021-24814-1","article-title":"The apicoplast link to fever-survival and artemisinin-resistance in the malaria parasite","volume":"12","author":"Zhang","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2024.109602_bib60","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.2307\/3280864","article-title":"Culture of Plasmodium falciparum: the role of pH, glucose, and lactate","volume":"69","author":"Jensen","year":"1983","journal-title":"J.\u00a0Parasitol."},{"key":"10.1016\/j.isci.2024.109602_bib61","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1111\/mmi.14441","article-title":"Autophagy-related protein PfATG18 participates in food vacuole dynamics and autophagy-like pathway in Plasmodium falciparum","volume":"113","author":"Agrawal","year":"2020","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.isci.2024.109602_bib62","doi-asserted-by":"crossref","first-page":"790067","DOI":"10.3389\/fcimb.2021.790067","article-title":"Stressed Out About Gametocytogenesis","volume":"11","author":"Usui","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.isci.2024.109602_bib63","doi-asserted-by":"crossref","first-page":"2917","DOI":"10.1016\/j.celrep.2019.10.095","article-title":"Decreased K13 Abundance Reduces Hemoglobin Catabolism and Proteotoxic Stress, Underpinning Artemisinin Resistance","volume":"29","author":"Yang","year":"2019","journal-title":"Cell Rep."},{"key":"10.1016\/j.isci.2024.109602_bib64","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1093\/infdis\/jix156","article-title":"Multi-omics Based Identification of Specific Biochemical Changes Associated With PfKelch13-Mutant Artemisinin-Resistant Plasmodium falciparum","volume":"215","author":"Siddiqui","year":"2017","journal-title":"J.\u00a0Infect. Dis."},{"key":"10.1016\/j.isci.2024.109602_bib65","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1126\/science.aax4735","article-title":"A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malaria parasites","volume":"367","author":"Birnbaum","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.isci.2024.109602_bib66","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1038\/nature12970","article-title":"A cascade of DNA-binding proteins for sexual commitment and development in Plasmodium","volume":"507","author":"Sinha","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.isci.2024.109602_bib67","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.chom.2014.07.004","article-title":"Heterochromatin protein 1 secures survival and transmission of malaria parasites","volume":"16","author":"Brancucci","year":"2014","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.isci.2024.109602_bib68","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1146\/annurev-biochem-060815-014607","article-title":"CRISPR\/Cas9 in Genome Editing and Beyond","volume":"85","author":"Wang","year":"2016","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.isci.2024.109602_bib69","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/nbt.3199","article-title":"Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers","volume":"33","author":"Hilton","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.isci.2024.109602_bib70","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1073\/pnas.1813542116","article-title":"Epigenetic editing by CRISPR\/dCas9 in Plasmodium falciparum","volume":"116","author":"Xiao","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2024.109602_bib71","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1038\/cr.2013.122","article-title":"Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system","volume":"23","author":"Cheng","year":"2013","journal-title":"Cell Res."},{"key":"10.1016\/j.isci.2024.109602_bib72","article-title":"Empower multiplex cell and tissue-specific CRISPR-mediated gene manipulation with self-cleaving ribozymes and tRNA","volume":"45","author":"Xu","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2024.109602_bib73","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s00284-017-1406-8","article-title":"The Conspicuity of CRISPR-Cpf1 System as a Significant Breakthrough in Genome Editing","volume":"75","author":"Bayat","year":"2018","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.isci.2024.109602_bib74","doi-asserted-by":"crossref","DOI":"10.1002\/wrna.1481","article-title":"Cas9 versus Cas12a\/Cpf1: Structure-function comparisons and implications for genome editing","volume":"9","author":"Swarts","year":"2018","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"10.1016\/j.isci.2024.109602_bib75","doi-asserted-by":"crossref","first-page":"3918","DOI":"10.1093\/nar\/gkad248","article-title":"Characterization of the dual role of Plasmodium falciparum DNA methyltransferase in regulating transcription and translation","volume":"51","author":"Lucky","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2024.109602_bib76","doi-asserted-by":"crossref","DOI":"10.1038\/s42003-023-05038-z","article-title":"A type II protein arginine methyltransferase regulates merozoite invasion in Plasmodium falciparum","volume":"6","author":"Lucky","year":"2023","journal-title":"Commun. Biol."},{"key":"10.1016\/j.isci.2024.109602_bib77","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1038\/nbt.2925","article-title":"Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system","volume":"32","author":"Ghorbal","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.isci.2024.109602_bib78","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.isci.2024.109602_bib79","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1093\/nar\/29.3.850","article-title":"Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes","volume":"29","author":"Deitsch","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2024.109602_bib80","first-page":"S1791","article-title":"Faster single-end alignment generation utilizing multi-thread for BWA","volume":"26","author":"Jo","year":"2015","journal-title":"Bio Med. Mater. Eng."},{"key":"10.1016\/j.isci.2024.109602_bib81","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004224008241?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004224008241?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:38:29Z","timestamp":1783384709000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004224008241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":81,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["S2589004224008241"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2024.109602","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Transforming the CRISPR\/dCas9-based gene regulation technique into a forward screening tool in Plasmodium falciparum","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2024.109602","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"109602"}}