{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:43:20Z","timestamp":1792853000128,"version":"4.5.0"},"reference-count":78,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T00:00:00Z","timestamp":1721952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["SBIR-R43AI149804"],"award-info":[{"award-number":["SBIR-R43AI149804"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2024,9]]},"DOI":"10.1016\/j.isci.2024.110618","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T22:23:02Z","timestamp":1722378182000},"page":"110618","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"title":["A novel pan-proteome array for high-throughput profiling of the humoral response to Treponema pallidum"],"prefix":"10.1016","volume":"27","author":[{"given":"Joseph J.","family":"Campo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Romeis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Oberai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jozelyn V.","family":"Pablo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy A.","family":"Teng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam D.","family":"Shandling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber","family":"Phan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin M.","family":"Haynes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3889-7274","authenticated-orcid":false,"given":"Lorenzo","family":"Giacani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2024.110618_bib1","series-title":"Prevalence and Incidence of Selected Sexually Transmitted Infections Chlamydia trachomatis, Neisseria Gonorrhoeae, Syphilis and Trichomonas vaginalis: Methods and Results Used by WHO to Generate 2005 Estimates","year":"2011"},{"key":"10.1016\/j.isci.2024.110618_bib2","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1016\/S0140-6736(98)90001-0","article-title":"Global epidemiology of sexually transmitted diseases","volume":"351","author":"Gerbase","year":"1998","journal-title":"Lancet"},{"key":"10.1016\/j.isci.2024.110618_bib3","series-title":"2022 Sexually Transmitted Disease Surveillance","year":"2024"},{"key":"10.1016\/j.isci.2024.110618_bib4","doi-asserted-by":"crossref","DOI":"10.2807\/ese.17.29.20224-en","article-title":"Rapid increase in gonorrhoea and syphilis diagnoses in England in 2011","volume":"17","author":"Savage","year":"2012","journal-title":"Euro Surveill."},{"key":"10.1016\/j.isci.2024.110618_bib5","doi-asserted-by":"crossref","DOI":"10.2807\/ese.14.47.19417-en","article-title":"Syphilis and gonorrhoea in men who have sex with men: a European overview","volume":"14","author":"Savage","year":"2009","journal-title":"Euro Surveill."},{"key":"10.1016\/j.isci.2024.110618_bib6","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1097\/01.olq.0000149848.03733.c1","article-title":"The re-emergence of syphilis in the United Kingdom: the new epidemic phases","volume":"32","author":"Simms","year":"2005","journal-title":"Sex. Transm. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib7","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1097\/QCO.0b013e32834204bf","article-title":"China's syphilis epidemic: epidemiology, proximate determinants of spread, and control responses","volume":"24","author":"Tucker","year":"2011","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib8","doi-asserted-by":"crossref","first-page":"179","DOI":"10.5694\/j.1326-5377.2005.tb06989.x","article-title":"Epidemic syphilis among homosexually active men in Sydney","volume":"183","author":"Jin","year":"2005","journal-title":"Med. J. Aust."},{"key":"10.1016\/j.isci.2024.110618_bib9","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1128\/CMR.19.1.29-49.2006","article-title":"Biological basis for syphilis","volume":"19","author":"LaFond","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.isci.2024.110618_bib10","series-title":"Manual of Tests for Syphilis","author":"Larsen","year":"1999"},{"key":"10.1016\/j.isci.2024.110618_bib11","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1128\/iai.24.3.713-719.1979","article-title":"Unique lipid composition of Treponema pallidum (Nichols virulent strain)","volume":"24","author":"Matthews","year":"1979","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.110618_bib12","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1097\/OLQ.0000000000001387","article-title":"The Terms \"Serofast\" and \"Serological Nonresponse\" in the Modern Syphilis Era","volume":"48","author":"Ghanem","year":"2021","journal-title":"Sex. Transm. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib13","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1309\/9TCQ-B1TA-38MV-R6UM","article-title":"Evaluation of the passive particle agglutination test in the serodiagnosis and follow-up of syphilis","volume":"116","author":"Castro","year":"2001","journal-title":"Am. J. Clin. Pathol."},{"key":"10.1016\/j.isci.2024.110618_bib14","doi-asserted-by":"crossref","first-page":"197","DOI":"10.4049\/jimmunol.117.1.197","article-title":"Humoral immunity in experimental syphilis. II. The relationship of neutralizing factors in immune serum to acquired resistance","volume":"117","author":"Bishop","year":"1976","journal-title":"J. Immunol."},{"key":"10.1016\/j.isci.2024.110618_bib15","doi-asserted-by":"crossref","first-page":"191","DOI":"10.4049\/jimmunol.117.1.191","article-title":"Humoral immunity in experimental syphilis. I. The demonstration of resistance conferred by passive immunization","volume":"117","author":"Bishop","year":"1976","journal-title":"J. Immunol."},{"key":"10.1016\/j.isci.2024.110618_bib16","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/cid\/ciab287","article-title":"Previous Syphilis Alters the Course of Subsequent Episodes of Syphilis","volume":"74","author":"Marra","year":"2022","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib17","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofy096","article-title":"Repeat Syphilis Is More Likely to Be Asymptomatic in HIV-Infected Individuals: A Retrospective Cohort Analysis With Important Implications for Screening","volume":"5","author":"Kenyon","year":"2018","journal-title":"Open Forum Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib18","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2018-021544","article-title":"A retrospective study of the clinical features of new syphilis infections in an HIV-positive cohort in Alberta, Canada","volume":"8","author":"Lang","year":"2018","journal-title":"BMJ Open"},{"key":"10.1016\/j.isci.2024.110618_bib19","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1093\/cid\/cix904","article-title":"Increases in Asymptomatic Early Syphilis May Reflect Increases in Repeated Episodes of Syphilis and Not Enhanced Screening","volume":"66","author":"Kenyon","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib20","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1136\/sextrans-2017-053312","article-title":"Repeat syphilis has a different immune response compared with initial syphilis: an analysis of biomarker kinetics in two cohorts","volume":"94","author":"Kenyon","year":"2018","journal-title":"Sex. Transm. Infect."},{"key":"10.1016\/j.isci.2024.110618_bib21","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.plasmid.2018.09.011","article-title":"Linezolid resistance genes and genetic elements enhancing their dissemination in enterococci and streptococci","volume":"99","author":"Sadowy","year":"2018","journal-title":"Plasmid"},{"key":"10.1016\/j.isci.2024.110618_bib22","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/infdis\/165.1.69","article-title":"Macrophage-mediated killing of opsonized Treponema pallidum","volume":"165","author":"Baker-Zander","year":"1992","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib23","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1093\/infdis\/166.6.1449","article-title":"A subpopulation of Treponema pallidum is resistant to phagocytosis: possible mechanism of persistence","volume":"166","author":"Lukehart","year":"1992","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib24","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1574-695X.1993.tb00339.x","article-title":"Characterization of the serum requirement for macrophage-mediated killing of Treponema pallidum ssp. pallidum: relationship to the development of opsonizing antibodies","volume":"6","author":"Baker-Zander","year":"1993","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib25","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010063","article-title":"Treponema pallidum genome sequencing from six continents reveals variability in vaccine candidate genes and dominance of Nichols clade strains in Madagascar","volume":"15","author":"Lieberman","year":"2021","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib26","doi-asserted-by":"crossref","first-page":"6499","DOI":"10.1128\/JB.187.18.6499-6508.2005","article-title":"TP0453, a concealed outer membrane protein of Treponema pallidum, enhances membrane permeability","volume":"187","author":"Hazlett","year":"2005","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.isci.2024.110618_bib27","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2020.621654","article-title":"Identification and Functional Assessment of the First Placental Adhesin of Treponema pallidum That May Play Critical Role in Congenital Syphilis","volume":"11","author":"Primus","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib28","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003662","article-title":"Treponema pallidum subsp. pallidum TP0136 protein is heterogeneous among isolates and binds cellular and plasma fibronectin via its NH2-terminal end","volume":"9","author":"Ke","year":"2015","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib29","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1086\/656533","article-title":"Enhanced molecular typing of Treponema pallidum: geographical distribution of strain types and association with neurosyphilis","volume":"202","author":"Marra","year":"2010","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib30","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1128\/IAI.01124-06","article-title":"Quantitative analysis of tpr gene expression in Treponema pallidum isolates: Differences among isolates and correlation with T-cell responsiveness in experimental syphilis","volume":"75","author":"Giacani","year":"2007","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.110618_bib31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1009949","article-title":"Comparison of transcriptional profiles of Treponema pallidum during experimental infection of rabbits and in vitro culture: Highly similar, yet different","volume":"17","author":"De Lay","year":"2021","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.isci.2024.110618_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002222","article-title":"Fine Analysis of Genetic Diversity of the tpr Gene Family among Treponemal Species, Subspecies and Strains","volume":"7","author":"Centurion-Lara","year":"2013","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib33","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12900-018-0086-3","article-title":"Functional insights from proteome-wide structural modeling of Treponema pallidum subspecies pallidum, the causative agent of syphilis","volume":"18","author":"Houston","year":"2018","journal-title":"BMC Struct. Biol."},{"key":"10.1016\/j.isci.2024.110618_bib34","doi-asserted-by":"crossref","first-page":"2631","DOI":"10.1128\/IAI.00173-10","article-title":"Characterization and serologic analysis of the Treponema pallidum proteome","volume":"78","author":"McGill","year":"2010","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.110618_bib35","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-45219-8","article-title":"Deep proteome coverage advances knowledge of Treponema pallidum protein expression profiles during infection","volume":"13","author":"Houston","year":"2023","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2024.110618_bib36","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0074319","article-title":"Resequencing of Treponema pallidum ssp. pallidum strains Nichols and SS14: correction of sequencing errors resulted in increased separation of syphilis treponeme subclusters","volume":"8","author":"Petrosova","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2024.110618_bib37","doi-asserted-by":"crossref","unstructured":"Romeis E, Lieberman NAP, Molini B,Tantalo, L.C., Chung, B., Phung, Q., Avenda\u00f1o, C., Vorobieva, A., Greninger, A.L. Giacani, L. Treponema pallidum subsp. pallidum with an Artificially Impaired TprK Antigenic Variation System is Attenuated in the Rabbit Model of Syphilis. PLoS Pathog.,19(3), p.e1011259","DOI":"10.1371\/journal.ppat.1011259"},{"key":"10.1016\/j.isci.2024.110618_bib38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009753","article-title":"Longitudinal TprK profiling of in vivo and in vitro-propagated Treponema pallidum subsp. pallidum reveals accumulation of antigenic variants in absence of immune pressure","volume":"15","author":"Lin","year":"2021","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib39","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007921","article-title":"Comparative genomics and full-length TprK profiling of Treponema pallidum subsp. pallidum reinfection","volume":"14","author":"Addetia","year":"2020","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib40","doi-asserted-by":"crossref","first-page":"4445","DOI":"10.1128\/IAI.73.7.4445-4450.2005","article-title":"Genome scale identification of Treponema pallidum antigens","volume":"73","author":"McKevitt","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.110618_bib41","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1126\/science.281.5375.375","article-title":"Complete genome sequence of Treponema pallidum, the syphilis spirochete","volume":"281","author":"Fraser","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.isci.2024.110618_bib42","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1128\/iai.65.4.1440-1444.1997","article-title":"Conservation of the 15-kilodalton lipoprotein among Treponema pallidum subspecies and strains and other pathogenic treponemes: genetic and antigenic analyses","volume":"65","author":"Centurion-Lara","year":"1997","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.110618_bib43","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1590\/S0036-46651999000200009","article-title":"Analysis of Treponema pallidum recombinant antigens for diagnosis of syphilis by western blotting technique","volume":"41","author":"Sato","year":"1999","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"10.1016\/j.isci.2024.110618_bib44","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1128\/jcm.32.3.629-633.1994","article-title":"Analysis of western blotting (immunoblotting) technique in diagnosis of congenital syphilis","volume":"32","author":"Meyer","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib45","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1515\/CCLM.2009.071","article-title":"Sensitive and specific ELISA coated by TpN15-TpN17-TpN47 fusion protein for detection of antibodies to Treponema pallidum","volume":"47","author":"Sun","year":"2009","journal-title":"Clin. Chem. Lab. Med."},{"key":"10.1016\/j.isci.2024.110618_bib46","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1097\/OLQ.0b013e31819db594","article-title":"Evaluation of immunoglobulin M and G Western blot and ELISA for screening antibodies to Treponema pallidum in blood donors","volume":"36","author":"Wang","year":"2009","journal-title":"Sex. Transm. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib47","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.diagmicrobio.2006.12.024","article-title":"Characterization of the Western blotting IgG reactivity patterns in the clinical phases of acquired syphilis","volume":"58","author":"de Lemos","year":"2007","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib48","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1046\/j.1469-0691.2001.00232.x","article-title":"Evaluation of recomWell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis","volume":"7","author":"Sambri","year":"2001","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.isci.2024.110618_bib49","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0732-8893(00)00213-3","article-title":"Treponema pallidum western blot: comparison with the FTA-ABS test as a confirmatory test for syphilis","volume":"39","author":"Backhouse","year":"2001","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib50","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1258\/0956462981922034","article-title":"A new recombinant antigen latex agglutination test (Syphilis Fast) for the rapid serological diagnosis of syphilis","volume":"9","author":"Young","year":"1998","journal-title":"Int. J. STD AIDS"},{"key":"10.1016\/j.isci.2024.110618_bib51","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1128\/JCM.36.4.913-917.1998","article-title":"Novel recombinant-antigen enzyme immunoassay for serological diagnosis of syphilis","volume":"36","author":"Young","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib52","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1128\/JCM.44.3.888-891.2006","article-title":"Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome","volume":"44","author":"Brinkman","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib53","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.03466-23","article-title":"Evaluation of a minimal array of Treponema pallidum antigens as biomarkers for syphilis diagnosis, infection staging, and response to treatment","volume":"12","author":"Haynes","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.isci.2024.110618_bib54","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1128\/jcm.27.1.152-157.1989","article-title":"Sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant DNA-derived Treponema pallidum protein TmpA for serodiagnosis of syphilis and the potential use of TmpA for assessing the effect of antibiotic therapy","volume":"27","author":"Ijsselmuiden","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib55","doi-asserted-by":"crossref","first-page":"2612","DOI":"10.1128\/iai.57.9.2612-2623.1989","article-title":"Overproduction and purification of Treponema pallidum recombinant-DNA-derived proteins TmpA and TmpB and their potential use in serodiagnosis of syphilis","volume":"57","author":"Schouls","year":"1989","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.110618_bib56","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s10517-018-4261-0","article-title":"Immunochip for Syphilis Serodiagnostics with the Use of Extended Array of Treponema pallidum Recombinant Antigens","volume":"165","author":"Runina","year":"2018","journal-title":"Bull. Exp. Biol. Med."},{"key":"10.1016\/j.isci.2024.110618_bib57","doi-asserted-by":"crossref","DOI":"10.1155\/2017\/1436080","article-title":"Novel Treponema pallidum Recombinant Antigens for Syphilis Diagnostics: Current Status and Future Prospects","volume":"2017","author":"Kubanov","year":"2017","journal-title":"BioMed Res. Int."},{"key":"10.1016\/j.isci.2024.110618_bib58","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.03536-20","article-title":"Parameters Affecting Continuous In Vitro Culture of Treponema pallidum Strains","volume":"12","author":"Edmondson","year":"2021","journal-title":"mBio"},{"key":"10.1016\/j.isci.2024.110618_bib59","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1177\/09564624211042444","article-title":"Countering the rise of syphilis: A role for doxycycline post-exposure prophylaxis?","volume":"33","author":"Tran","year":"2022","journal-title":"Int. J. STD AIDS"},{"key":"10.1016\/j.isci.2024.110618_bib60","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1093\/cid\/ciz866","article-title":"Doxycycline Prophylaxis for Bacterial Sexually Transmitted Infections","volume":"70","author":"Grant","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib61","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/S1473-3099(17)30725-9","article-title":"Post-exposure prophylaxis with doxycycline to prevent sexually transmitted infections in men who have sex with men: an open-label randomised substudy of the ANRS IPERGAY trial","volume":"18","author":"Molina","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.isci.2024.110618_bib62","series-title":"Biology of the Treponematoses","author":"Turner","year":"1957"},{"key":"10.1016\/j.isci.2024.110618_bib63","first-page":"18","article-title":"The effect of the method of inoculation on the course of experimental syphilis in the rabbit","volume":"34","author":"Chesney","year":"1950","journal-title":"American J. Syphilis"},{"key":"10.1016\/j.isci.2024.110618_bib64","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.4049\/jimmunol.121.5.2014","article-title":"Demonstration of the in vitro phagocytosis of Treponema pallidum by rabbit peritoneal macrophages","volume":"121","author":"Lukehart","year":"1978","journal-title":"J. Immunol."},{"key":"10.1016\/j.isci.2024.110618_bib65","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1111\/j.1365-2958.2011.07662.x","article-title":"TP0326, a Treponema pallidum beta-barrel assembly machinery A (BamA) orthologue and rare outer membrane protein","volume":"80","author":"Desrosiers","year":"2011","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib66","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1222267","article-title":"Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326)","volume":"14","author":"Ferguson","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2024.110618_bib67","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00323-19","article-title":"Evaluation of the Protective Ability of the Treponema pallidum subsp. pallidum Tp0126 OmpW Homolog in the Rabbit Model of Syphilis","volume":"87","author":"Haynes","year":"2019","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.110618_bib68","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1128\/IAI.01424-07","article-title":"A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin","volume":"76","author":"Brinkman","year":"2008","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2024.110618_bib69","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.759474","article-title":"Two Potential Syphilis Vaccine Candidates Inhibit Dissemination of Treponema pallidum","volume":"12","author":"Xu","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2024.110618_bib70","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1574-695X.2007.00303.x","article-title":"Length of guanosine homopolymeric repeats modulates promoter activity of Subfamily II tpr genes of Treponema pallidum ssp. pallidum","volume":"51","author":"Giacani","year":"2007","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib71","doi-asserted-by":"crossref","first-page":"12","DOI":"10.4056\/sigs.2615838","article-title":"Complete genome sequence of Treponema pallidum strain DAL-1","volume":"7","author":"Zobanikov\u00e1","year":"2012","journal-title":"Stand Genomic Sci."},{"key":"10.1016\/j.isci.2024.110618_bib72","first-page":"Unit 12A.1","article-title":"Isolation and laboratory maintenance of Treponema pallidum","volume":"Chapter 12","author":"Lukehart","year":"2007","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.isci.2024.110618_bib73","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.862491","article-title":"B-Cell Epitope Mapping of TprC and TprD Variants of Treponema pallidum Subspecies Informs Vaccine Development for Human Treponematoses","volume":"13","author":"Molini","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2024.110618_bib74","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1093\/bioinformatics\/btw313","article-title":"Complex heatmaps reveal patterns and correlations in multidimensional genomic data","volume":"32","author":"Gu","year":"2016","journal-title":"Bioinformatics"},{"key":"10.1016\/j.isci.2024.110618_bib75","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1093\/bioinformatics\/btx364","article-title":"UpSetR: an R package for the visualization of intersecting sets and their properties","volume":"33","author":"Conway","year":"2017","journal-title":"Bioinformatics"},{"key":"10.1016\/j.isci.2024.110618_bib76","series-title":"Linear Programming Algorithms for Detecting Separated Data in Binary Logistic Regression Models","author":"Konis","year":"2007"},{"key":"10.1016\/j.isci.2024.110618_bib77","doi-asserted-by":"crossref","DOI":"10.1038\/psp.2013.56","article-title":"Application of ggplot2 to Pharmacometric Graphics","volume":"2","author":"Ito","year":"2013","journal-title":"CPT Pharmacometrics Syst. Pharmacol."},{"key":"10.1016\/j.isci.2024.110618_bib78","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: a practical and powerful approach to multiple testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. Roy. Statist. Soc. Ser."}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004224018431?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004224018431?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T19:44:29Z","timestamp":1762631069000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004224018431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":78,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["S2589004224018431"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2024.110618","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel pan-proteome array for high-throughput profiling of the humoral response to Treponema pallidum","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2024.110618","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"110618"}}