{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:24:37Z","timestamp":1786991077081,"version":"build-2736575974"},"reference-count":77,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["Q0035"],"award-info":[{"award-number":["Q0035"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["R01-NS129847"],"award-info":[{"award-number":["R01-NS129847"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01-NS129847"],"award-info":[{"award-number":["R01-NS129847"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Institutes of Health National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2022-02982"],"award-info":[{"award-number":["RGPIN-2022-02982"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY026545"],"award-info":[{"award-number":["EY026545"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01-EY031949"],"award-info":[{"award-number":["R01-EY031949"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.isci.2025.112371","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T16:17:09Z","timestamp":1744129029000},"page":"112371","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium"],"prefix":"10.1016","volume":"28","author":[{"given":"Feng","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph M.","family":"Nichols","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melina A.","family":"Agosto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore G.","family":"Wensel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2025.112371_bib1","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S1534-5807(04)00099-1","article-title":"Development by self-digestion: molecular mechanisms and biological functions of autophagy","volume":"6","author":"Levine","year":"2004","journal-title":"Dev. Cell"},{"key":"10.1016\/j.isci.2025.112371_bib2","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1083\/jcb.200907014","article-title":"Coordination of membrane events during autophagy by multiple class III PI3-kinase complexes","volume":"186","author":"Simonsen","year":"2009","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.112371_bib3","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1242\/jcs.02855","article-title":"Regulation of membrane traffic by phosphoinositide 3-kinases","volume":"119","author":"Lindmo","year":"2006","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.isci.2025.112371_bib4","doi-asserted-by":"crossref","first-page":"4930","DOI":"10.1093\/emboj\/17.17.4930","article-title":"Phosphoinositide signaling and turnover: PtdIns(3)P, a regulator of membrane traffic, is transported to the vacuole and degraded by a process that requires lumenal vacuolar hydrolase activities","volume":"17","author":"Wurmser","year":"1998","journal-title":"EMBO J."},{"key":"10.1016\/j.isci.2025.112371_bib5","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1242\/jcs.02735","article-title":"Functional specificity of the mammalian Beclin-Vps34 PI 3-kinase complex in macroautophagy versus endocytosis and lysosomal enzyme trafficking","volume":"119","author":"Zeng","year":"2006","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.isci.2025.112371_bib6","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1083\/jcb.200107069","article-title":"Distinct roles of class I and class III phosphatidylinositol 3-kinases in phagosome formation and maturation","volume":"155","author":"Vieira","year":"2001","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.112371_bib7","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/ncomms1037","article-title":"PI(3,5)P(2) controls membrane trafficking by direct activation of mucolipin Ca(2+) release channels in the endolysosome","volume":"1","author":"Dong","year":"2010","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2025.112371_bib8","doi-asserted-by":"crossref","first-page":"4868","DOI":"10.1093\/hmg\/ddp460","article-title":"Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2","volume":"18","author":"Ferguson","year":"2009","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.isci.2025.112371_bib9","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1042\/BST20150174","article-title":"Phosphatidylinositol 3,5-bisphosphate: regulation of cellular events in space and time","volume":"44","author":"Jin","year":"2016","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.isci.2025.112371_bib10","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.REV120.014405","article-title":"Pathways and disease-causing alterations in visual chromophore production for vertebrate vision","volume":"296","author":"Kiser","year":"2021","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.112371_bib11","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/978-94-024-0945-1_9","article-title":"Vitamin A and Vision","volume":"81","author":"Saari","year":"2016","journal-title":"Subcell. Biochem."},{"key":"10.1016\/j.isci.2025.112371_bib12","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/bs.pmbts.2015.06.009","article-title":"A History of the Classical Visual Cycle","volume":"134","author":"Wright","year":"2015","journal-title":"Prog. Mol. Biol. Transl. Sci."},{"key":"10.1016\/j.isci.2025.112371_bib13","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1152\/physiol.00038.2009","article-title":"Phagocytosis of retinal rod and cone photoreceptors","volume":"25","author":"Kevany","year":"2010","journal-title":"Physiology"},{"key":"10.1016\/j.isci.2025.112371_bib14","first-page":"397","article-title":"Outer segment disc shedding and phagocytosis in the outer retina","volume":"103","author":"LaVail","year":"1983","journal-title":"Trans. Ophthalmol. Soc. UK"},{"key":"10.1016\/j.isci.2025.112371_bib15","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1126\/science.982063","article-title":"Rod outer segment disk shedding in rat retina: relationship to cyclic lighting","volume":"194","author":"LaVail","year":"1976","journal-title":"Science"},{"key":"10.1016\/j.isci.2025.112371_bib16","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1083\/jcb.42.2.392","article-title":"Participation of the retinal pigment epithelium in the rod outer segment renewal process","volume":"42","author":"Young","year":"1969","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.112371_bib17","doi-asserted-by":"crossref","first-page":"9014","DOI":"10.1073\/pnas.1805039115","article-title":"Aberrant early endosome biogenesis mediates complement activation in the retinal pigment epithelium in models of macular degeneration","volume":"115","author":"Kaur","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2025.112371_bib18","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/s42003-021-02386-6","article-title":"\u03b2A3\/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells","volume":"4","author":"Shang","year":"2021","journal-title":"Commun. Biol."},{"key":"10.1016\/j.isci.2025.112371_bib19","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1083\/jcb.100.5.1676","article-title":"Endocytosis and degradation of interstitial retinol-binding protein: differential capabilities of cells that border the interphotoreceptor matrix","volume":"100","author":"Hollyfield","year":"1985","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.112371_bib20","doi-asserted-by":"crossref","first-page":"7199","DOI":"10.1111\/febs.16018","article-title":"Autophagy in the retinal pigment epithelium: a new vision and future challenges","volume":"289","author":"Intartaglia","year":"2022","journal-title":"FEBS J."},{"key":"10.1016\/j.isci.2025.112371_bib21","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1080\/15548627.2022.2069437","article-title":"Autophagy in age-related macular degeneration","volume":"19","author":"Kaarniranta","year":"2023","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2025.112371_bib22","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms14819","article-title":"Retrograde transport of TrkB-containing autophagosomes via the adaptor AP-2 mediates neuronal complexity and prevents neurodegeneration","volume":"8","author":"Kononenko","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2025.112371_bib23","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1159\/000103376","article-title":"Expression of autophagy-associated genes in skeletal muscle: an experimental model of chloroquine-induced myopathy","volume":"74","author":"Kimura","year":"2007","journal-title":"Pathobiology"},{"key":"10.1016\/j.isci.2025.112371_bib24","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1038\/cdd.2009.54","article-title":"The late stages of autophagy: how does the end begin?","volume":"16","author":"Noda","year":"2009","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.isci.2025.112371_bib25","doi-asserted-by":"crossref","DOI":"10.3390\/cells10113124","article-title":"Activation Mechanisms of the VPS34 Complexes","volume":"10","author":"Ohashi","year":"2021","journal-title":"Cells"},{"key":"10.1016\/j.isci.2025.112371_bib26","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1042\/BST0370232","article-title":"Nutrient-dependent regulation of autophagy through the target of rapamycin pathway","volume":"37","author":"Chang","year":"2009","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.isci.2025.112371_bib27","doi-asserted-by":"crossref","first-page":"9424","DOI":"10.1073\/pnas.0914725107","article-title":"Deletion of PIK3C3\/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway","volume":"107","author":"Zhou","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2025.112371_bib28","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1167\/iovs.19-26586","article-title":"Critical Role for Phosphatidylinositol-3 Kinase Vps34\/PIK3C3 in ON-Bipolar Cells","volume":"60","author":"He","year":"2019","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.isci.2025.112371_bib29","article-title":"Phosphatidylinositol-3-phosphate is light-regulated and essential for survival in retinal rods","volume":"6","author":"He","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2025.112371_bib30","doi-asserted-by":"crossref","first-page":"707","DOI":"10.4161\/auto.19627","article-title":"Mammalian PIK3C3\/VPS34: the key to autophagic processing in liver and heart","volume":"8","author":"Jaber","year":"2012","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2025.112371_bib31","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.4161\/auto.24634","article-title":"The class III phosphatidylinositol 3-kinase PIK3C3\/VPS34 regulates endocytosis and autophagosome-autolysosome formation in podocytes","volume":"9","author":"Bechtel","year":"2013","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2025.112371_bib32","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1016\/j.ajpath.2014.02.012","article-title":"Conditional knockout of pik3c3 causes a murine muscular dystrophy","volume":"184","author":"Reifler","year":"2014","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.isci.2025.112371_bib33","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abn1702","article-title":"Beyond autophagy: LC3-associated phagocytosis and endocytosis","volume":"8","author":"Pena-Martinez","year":"2022","journal-title":"Sci. Adv."},{"key":"10.1016\/j.isci.2025.112371_bib34","doi-asserted-by":"crossref","first-page":"4577","DOI":"10.1093\/emboj\/19.17.4577","article-title":"Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells","volume":"19","author":"Gillooly","year":"2000","journal-title":"EMBO J."},{"key":"10.1016\/j.isci.2025.112371_bib35","doi-asserted-by":"crossref","first-page":"506","DOI":"10.4161\/auto.6.4.11863","article-title":"Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation","volume":"6","author":"Polson","year":"2010","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2025.112371_bib36","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0076405","article-title":"Regulation of mammalian autophagy by class II and III PI 3-kinases through PI3P synthesis","volume":"8","author":"Devereaux","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2025.112371_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1600-0854.2011.01246.x","article-title":"Phosphatidylinositol-3,5-bisphosphate: no longer the poor PIP2","volume":"13","author":"Ho","year":"2012","journal-title":"Traffic"},{"key":"10.1016\/j.isci.2025.112371_bib38","doi-asserted-by":"crossref","first-page":"3445","DOI":"10.1016\/j.visres.2005.07.016","article-title":"Rhodopsin-EGFP knock-ins for imaging quantal gene alterations","volume":"45","author":"Wensel","year":"2005","journal-title":"Vision Res."},{"key":"10.1016\/j.isci.2025.112371_bib39","doi-asserted-by":"crossref","first-page":"9109","DOI":"10.1073\/pnas.0403149101","article-title":"Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy","volume":"101","author":"Chan","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2025.112371_bib40","doi-asserted-by":"crossref","first-page":"3561","DOI":"10.1016\/j.jmb.2017.08.012","article-title":"LC3-Associated Phagocytosis and Inflammation","volume":"429","author":"Heckmann","year":"2017","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.isci.2025.112371_bib41","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.cell.2013.06.012","article-title":"Noncanonical autophagy promotes the visual cycle","volume":"154","author":"Kim","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.isci.2025.112371_bib42","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1073\/pnas.2235688100","article-title":"Electroporation and RNA interference in the rodent retina in vivo and in vitro","volume":"101","author":"Matsuda","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2025.112371_bib43","doi-asserted-by":"crossref","first-page":"7326","DOI":"10.1073\/pnas.94.14.7326","article-title":"Identification of an early endosomal protein regulated by phosphatidylinositol 3-kinase","volume":"94","author":"Patki","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2025.112371_bib44","doi-asserted-by":"crossref","first-page":"24048","DOI":"10.1074\/jbc.271.39.24048","article-title":"Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger","volume":"271","author":"Stenmark","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.112371_bib45","doi-asserted-by":"crossref","first-page":"13503","DOI":"10.1074\/jbc.270.22.13503","article-title":"EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine \"fingers\" and contains a calmodulin-binding IQ motif","volume":"270","author":"Mu","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.112371_bib46","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1021\/bi00272a020","article-title":"Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes","volume":"22","author":"Molday","year":"1983","journal-title":"Biochemistry"},{"key":"10.1016\/j.isci.2025.112371_bib47","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1126\/science.291.5509.1793","article-title":"Structural mechanism of endosome docking by the FYVE domain","volume":"291","author":"Kutateladze","year":"2001","journal-title":"Science"},{"key":"10.1016\/j.isci.2025.112371_bib48","doi-asserted-by":"crossref","first-page":"2255","DOI":"10.1242\/jcs.114.12.2255","article-title":"FYVE and coiled-coil domains determine the specific localisation of Hrs to early endosomes","volume":"114","author":"Raiborg","year":"2001","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.isci.2025.112371_bib49","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ceb.2009.11.014","article-title":"Mammalian autophagy: core molecular machinery and signaling regulation","volume":"22","author":"Yang","year":"2010","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.isci.2025.112371_bib50","first-page":"419","article-title":"Evidence against the role of rhodopsin in rod outer segment binding to RPE cells","volume":"29","author":"Laird","year":"1988","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.isci.2025.112371_bib51","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1167\/iovs.10-6347","article-title":"Generation of Cre transgenic mice with postnatal RPE-specific ocular expression","volume":"52","author":"Iacovelli","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.isci.2025.112371_bib52","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1016\/j.ajpath.2014.02.007","article-title":"Age- and gene-dosage-dependent cre-induced abnormalities in the retinal pigment epithelium","volume":"184","author":"He","year":"2014","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.isci.2025.112371_bib53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/15548627.2020.1797280","article-title":"Guidelines for the use and interpretation of assays for monitoring (4th edition)1","volume":"17","author":"Klionsky","year":"2021","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2025.112371_bib54","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1083\/jcb.201501038","article-title":"PtdIns(4,5)P2 and PtdIns3P coordinate to regulate phagosomal sealing for apoptotic cell clearance","volume":"210","author":"Cheng","year":"2015","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.112371_bib55","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.4161\/auto.26323","article-title":"Autophagy genes coordinate with the class II PI\/PtdIns 3-kinase PIKI-1 to regulate apoptotic cell clearance in C. elegans","volume":"9","author":"Cheng","year":"2013","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2025.112371_bib56","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1001245","article-title":"Two PI 3-kinases and one PI 3-phosphatase together establish the cyclic waves of phagosomal PtdIns(3)P critical for the degradation of apoptotic cells","volume":"10","author":"Lu","year":"2012","journal-title":"PLoS Biol."},{"key":"10.1016\/j.isci.2025.112371_bib57","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ab.2006.05.014","article-title":"Application of phosphoinositide-binding domains for the detection and quantification of specific phosphoinositides","volume":"355","author":"Furutani","year":"2006","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.isci.2025.112371_bib58","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1042\/BJ20140889","article-title":"Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase","volume":"463","author":"Bago","year":"2014","journal-title":"Biochem. J."},{"key":"10.1016\/j.isci.2025.112371_bib59","doi-asserted-by":"crossref","first-page":"3805","DOI":"10.1016\/j.cub.2023.07.031","article-title":"Optineurin tunes outside-in signaling to regulate lysosome biogenesis and phagocytic clearance in the retina","volume":"33","author":"Tan","year":"2023","journal-title":"Curr. Biol."},{"key":"10.1016\/j.isci.2025.112371_bib60","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0074-7696(00)96006-6","article-title":"Renewal of photoreceptor outer segments and their phagocytosis by the retinal pigment epithelium","volume":"196","author":"Nguyen-Legros","year":"2000","journal-title":"Int. Rev. Cytol."},{"key":"10.1016\/j.isci.2025.112371_bib61","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/imr.13264","article-title":"Clearance phagocytosis by the retinal pigment epithelial during photoreceptor outer segment renewal: Molecular mechanisms and relation to retinal inflammation","volume":"319","author":"Lieffrig","year":"2023","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.isci.2025.112371_bib62","article-title":"The cell biology of the retinal pigment epithelium","volume":"2020","author":"Lakkaraju","year":"2020","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.isci.2025.112371_bib63","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1080\/15548627.2017.1380124","article-title":"RUBCN\/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye","volume":"13","author":"Muniz-Feliciano","year":"2017","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2025.112371_bib64","doi-asserted-by":"crossref","first-page":"12502","DOI":"10.1096\/fj.202000612R","article-title":"MTOR-initiated metabolic switch and degeneration in the retinal pigment epithelium","volume":"34","author":"Go","year":"2020","journal-title":"FASEB J."},{"key":"10.1016\/j.isci.2025.112371_bib65","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.aag3315","article-title":"Phagocytosed photoreceptor outer segments activate mTORC1 in the retinal pigment epithelium","volume":"11","author":"Yu","year":"2018","journal-title":"Sci. Signal."},{"key":"10.1016\/j.isci.2025.112371_bib66","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1073\/pnas.1112848109","article-title":"Class III PI3K Vps34 plays an essential role in autophagy and in heart and liver function","volume":"109","author":"Jaber","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2025.112371_bib67","doi-asserted-by":"crossref","first-page":"27019","DOI":"10.1074\/jbc.M114.593780","article-title":"Oligomeric state of purified transient receptor potential melastatin-1 (TRPM1), a protein essential for dim light vision","volume":"289","author":"Agosto","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.112371_bib68","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1258\/0023677011911525","article-title":"A simple polymerase chain reaction assay for genotyping the retinal degeneration mutation (Pdeb(rd1)) in FVB\/N-derived transgenic mice","volume":"35","author":"Gimenez","year":"2001","journal-title":"Lab. Anim."},{"key":"10.1016\/j.isci.2025.112371_bib69","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1167\/iovs.12-9662","article-title":"The Rd8 mutation of the Crb1 gene is present in vendor lines of C57BL\/6N mice and embryonic stem cells, and confounds ocular induced mutant phenotypes","volume":"53","author":"Mattapallil","year":"2012","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.isci.2025.112371_bib70","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1042\/bj3510019","article-title":"Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities","volume":"351","author":"Dowler","year":"2000","journal-title":"Biochem. J."},{"key":"10.1016\/j.isci.2025.112371_bib71","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/S0960-9822(98)70135-6","article-title":"Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells","volume":"8","author":"Stauffer","year":"1998","journal-title":"Curr. Biol."},{"key":"10.1016\/j.isci.2025.112371_bib72","doi-asserted-by":"crossref","first-page":"6639","DOI":"10.1128\/MCB.25.15.6639-6648.2005","article-title":"ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation","volume":"25","author":"Pedeux","year":"2005","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.isci.2025.112371_bib73","doi-asserted-by":"crossref","first-page":"764","DOI":"10.4161\/auto.6.6.12709","article-title":"Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins","volume":"6","author":"Itakura","year":"2010","journal-title":"Autophagy"},{"key":"10.1016\/j.isci.2025.112371_bib74","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.isci.2025.112371_bib75","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/978-1-4939-2330-4_18","article-title":"Three-dimensional architecture of murine rod cilium revealed by cryo-EM","volume":"1271","author":"Wensel","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.isci.2025.112371_bib76","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/978-1-4939-8669-9_7","article-title":"Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture","volume":"1834","author":"Mazzoni","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.isci.2025.112371_bib77","doi-asserted-by":"crossref","DOI":"10.1523\/ENEURO.0143-18.2018","article-title":"A Large Endoplasmic Reticulum-Resident Pool of TRPM1 in Retinal ON-Bipolar Cells","volume":"5","author":"Agosto","year":"2018","journal-title":"eNeuro"}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004225006327?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004225006327?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T01:00:38Z","timestamp":1776906038000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004225006327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":77,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["S2589004225006327"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2025.112371","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2025.112371","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"112371"}}