{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T20:59:21Z","timestamp":1781643561588,"version":"3.54.5"},"reference-count":48,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T00:00:00Z","timestamp":1754265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["111-2634-F-006-009"],"award-info":[{"award-number":["111-2634-F-006-009"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["109-2634-F-006-021"],"award-info":[{"award-number":["109-2634-F-006-021"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["109-2320-B-006-019"],"award-info":[{"award-number":["109-2320-B-006-019"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 110-2634-F-006-018"],"award-info":[{"award-number":["MOST 110-2634-F-006-018"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.isci.2025.113317","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T15:11:51Z","timestamp":1754493111000},"page":"113317","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Depletion of tropomyosin 1.6 alleviates TGF-\u03b21-induced fibroblast activation by promoting the \u03b1-SMA-MMP9 matrix-degrading structure"],"prefix":"10.1016","volume":"28","author":[{"given":"Ching-Lung","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang-Hui","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Hsiang","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Ting","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang-Kao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuei-Liong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzyy-Yue","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Jer","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2025.113317_bib1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/s41586-020-2941-1","article-title":"Decoding myofibroblast origins in human kidney fibrosis","volume":"589","author":"Kuppe","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.isci.2025.113317_bib2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.2353\/ajpath.2010.090517","article-title":"Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis","volume":"176","author":"Humphreys","year":"2010","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.isci.2025.113317_bib3","doi-asserted-by":"crossref","DOI":"10.1038\/cddis.2016.402","article-title":"Inflammatory macrophages can transdifferentiate into myofibroblasts during renal fibrosis","volume":"7","author":"Meng","year":"2016","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.isci.2025.113317_bib4","doi-asserted-by":"crossref","first-page":"2468","DOI":"10.1016\/j.ajpath.2015.05.019","article-title":"Matrix-Stiffness-Regulated Inverse Expression of Kruppel-Like Factor 5 and Kruppel-Like Factor 4 in the Pathogenesis of Renal Fibrosis","volume":"185","author":"Chen","year":"2015","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.isci.2025.113317_bib5","doi-asserted-by":"crossref","first-page":"F695","DOI":"10.1152\/ajprenal.00684.2013","article-title":"Regulation of proximal tubular cell differentiation and proliferation in primary culture by matrix stiffness and ECM components","volume":"307","author":"Chen","year":"2014","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"10.1016\/j.isci.2025.113317_bib6","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20202033","article-title":"Targeting mechanosensitive MDM4 promotes lung fibrosis resolution in aged mice","volume":"218","author":"Qu","year":"2021","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.isci.2025.113317_bib7","doi-asserted-by":"crossref","DOI":"10.1038\/srep31707","article-title":"Cellular adhesome screen identifies critical modulators of focal adhesion dynamics, cellular traction forces and cell migration behaviour","volume":"6","author":"Fokkelman","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2025.113317_bib8","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1083\/jcb.200609116","article-title":"Two distinct modes of myosin assembly and dynamics during epithelial wound closure","volume":"176","author":"Tamada","year":"2007","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.113317_bib9","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1189\/jlb.0711335","article-title":"The myosin family: unconventional roles of actin-dependent molecular motors in immune cells","volume":"91","author":"Maravillas-Montero","year":"2012","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.isci.2025.113317_bib10","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/jcp.25879","article-title":"TGFbeta signaling and the control of myofibroblast differentiation: Implications for chronic inflammatory disorders","volume":"233","author":"Carthy","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.isci.2025.113317_bib11","doi-asserted-by":"crossref","first-page":"4895","DOI":"10.1167\/iovs.06-0118","article-title":"Contractility as a prerequisite for TGF-beta-induced myofibroblast transdifferentiation in human tenon fibroblasts","volume":"47","author":"Meyer-ter-Vehn","year":"2006","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.isci.2025.113317_bib12","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1046\/j.1523-1755.2002.00328.x","article-title":"Y-27632 prevents tubulointerstitial fibrosis in mouse kidneys with unilateral ureteral obstruction","volume":"61","author":"Nagatoya","year":"2002","journal-title":"Kidney Int."},{"key":"10.1016\/j.isci.2025.113317_bib13","doi-asserted-by":"crossref","first-page":"6083","DOI":"10.1074\/jbc.M115.712380","article-title":"Matrix-driven Myosin II Mediates the Pro-fibrotic Fibroblast Phenotype","volume":"291","author":"Southern","year":"2016","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.113317_bib14","first-page":"2965","article-title":"Tropomyosin - master regulator of actin filament function in the cytoskeleton","volume":"128","author":"Gunning","year":"2015","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.isci.2025.113317_bib15","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.ejcb.2005.12.005","article-title":"Tropomyosins are present in lamellipodia of motile cells","volume":"85","author":"Hillberg","year":"2006","journal-title":"Eur. J. Cell Biol."},{"key":"10.1016\/j.isci.2025.113317_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2022.104484","article-title":"Distinct actin-tropomyosin cofilament populations drive the functional diversification of cytoskeletal myosin motor complexes","volume":"25","author":"Reindl","year":"2022","journal-title":"iScience"},{"key":"10.1016\/j.isci.2025.113317_bib17","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/978-0-387-85766-4_15","article-title":"Isoform sorting of tropomyosins","volume":"644","author":"Martin","year":"2008","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.isci.2025.113317_bib18","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.cub.2017.01.018","article-title":"Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations In Vitro","volume":"27","author":"Gateva","year":"2017","journal-title":"Curr. Biol."},{"key":"10.1016\/j.isci.2025.113317_bib19","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/cm.21230","article-title":"Stable incorporation of alpha-smooth muscle actin into stress fibers is dependent on specific tropomyosin isoforms","volume":"72","author":"Prunotto","year":"2015","journal-title":"Cytoskeleton (Hoboken)"},{"key":"10.1016\/j.isci.2025.113317_bib20","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/s12931-021-01730-y","article-title":"Tropomyosin 2.1 collaborates with fibronectin to promote TGF-\u03b21-induced contraction of human lung fibroblasts","volume":"22","author":"Bradbury","year":"2021","journal-title":"Respir. Res."},{"key":"10.1016\/j.isci.2025.113317_bib21","doi-asserted-by":"crossref","first-page":"13243","DOI":"10.1074\/jbc.M800927200","article-title":"Integrins uncouple Src-induced morphological and oncogenic transformation","volume":"283","author":"Huveneers","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.113317_bib22","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1242\/jcs.00839","article-title":"Actin cytoskeleton remodelling via local inhibition of contractility at discrete microdomains","volume":"117","author":"Burgstaller","year":"2004","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.isci.2025.113317_bib23","doi-asserted-by":"crossref","first-page":"8809","DOI":"10.18632\/oncotarget.6604","article-title":"TGF-beta\/Smad3 signalling regulates the transition of bone marrow-derived macrophages into myofibroblasts during tissue fibrosis","volume":"7","author":"Wang","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.isci.2025.113317_bib24","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.biomaterials.2018.08.039","article-title":"Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging","volume":"183","author":"Bennink","year":"2018","journal-title":"Biomaterials"},{"key":"10.1016\/j.isci.2025.113317_bib25","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms14913","article-title":"Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides","volume":"8","author":"Zitnay","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2025.113317_bib26","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aan5174","article-title":"Tissue-resident mesenchymal stromal cells: Implications for tissue-specific antifibrotic therapies","volume":"10","author":"Lemos","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.isci.2025.113317_bib27","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.1172\/JCI72267","article-title":"Cellular and molecular mechanisms in kidney fibrosis","volume":"124","author":"Duffield","year":"2014","journal-title":"J. Clin. Investig."},{"key":"10.1016\/j.isci.2025.113317_bib28","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/kisup.2014.6","article-title":"The FOXD1 lineage of kidney perivascular cells and myofibroblasts: functions and responses to injury","volume":"44","author":"Gomez","year":"2014","journal-title":"Kidney Int. Suppl."},{"key":"10.1016\/j.isci.2025.113317_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.mad.2021.111492","article-title":"Lens-specific conditional knockout of tropomyosin 1 gene in mice causes abnormal fiber differentiation and lens opacity","volume":"196","author":"Shibata","year":"2021","journal-title":"Mech. Ageing Dev."},{"key":"10.1016\/j.isci.2025.113317_bib30","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.tcb.2010.11.001","article-title":"Three's company: the fission yeast actin cytoskeleton","volume":"21","author":"Kovar","year":"2011","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.isci.2025.113317_bib31","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1083\/jcb.200110013","article-title":"Tropomyosin inhibits ADF\/cofilin-dependent actin filament dynamics","volume":"156","author":"Ono","year":"2002","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.113317_bib32","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1091\/mbc.e02-04-0244","article-title":"Specification of actin filament function and molecular composition by tropomyosin isoforms","volume":"14","author":"Bryce","year":"2003","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.isci.2025.113317_bib33","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.cub.2011.03.007","article-title":"A molecular pathway for myosin II recruitment to stress fibers","volume":"21","author":"Tojkander","year":"2011","journal-title":"Curr. Biol."},{"key":"10.1016\/j.isci.2025.113317_bib34","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1002\/cm.970290104","article-title":"Differential regulation of skeletal muscle myosin-II and brush border myosin-I enzymology and mechanochemistry by bacterially produced tropomyosin isoforms","volume":"29","author":"Fanning","year":"1994","journal-title":"Cell Motil Cytoskeleton"},{"key":"10.1016\/j.isci.2025.113317_bib35","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1242\/jcs.069971","article-title":"The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast","volume":"123","author":"Coulton","year":"2010","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.isci.2025.113317_bib36","doi-asserted-by":"crossref","first-page":"34832","DOI":"10.1074\/jbc.M104750200","article-title":"Modulation of myosin function by isoform-specific properties of Saccharomyces cerevisiae and muscle tropomyosins","volume":"276","author":"Strand","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.113317_bib37","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1002\/jmr.1093","article-title":"Systematic analysis of tropomodulin\/tropomyosin interactions uncovers fine-tuned binding specificity of intrinsically disordered proteins","volume":"24","author":"Uversky","year":"2011","journal-title":"J. Mol. Recognit."},{"key":"10.1016\/j.isci.2025.113317_bib38","doi-asserted-by":"crossref","first-page":"5066","DOI":"10.1074\/jbc.M311186200","article-title":"Effect of the structure of the N terminus of tropomyosin on tropomodulin function","volume":"279","author":"Kostyukova","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.113317_bib39","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.cub.2019.12.049","article-title":"Tropomodulins Control the Balance between Protrusive and Contractile Structures by Stabilizing Actin-Tropomyosin Filaments","volume":"30","author":"Kumari","year":"2020","journal-title":"Curr. Biol."},{"key":"10.1016\/j.isci.2025.113317_bib40","first-page":"5239","article-title":"Dynamic features of liver fibrogenesis and fibrosis resolution in the absence of matrix metalloproteinase-9","volume":"20","author":"Wang","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"10.1016\/j.isci.2025.113317_bib41","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21207597","article-title":"Influence of the TGF-beta Superfamily on Osteoclasts\/Osteoblasts Balance in Physiological and Pathological Bone Conditions","volume":"21","author":"Jann","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.isci.2025.113317_bib42","doi-asserted-by":"crossref","first-page":"3745","DOI":"10.1210\/en.2013-1272","article-title":"TGF-beta induces Wnt10b in osteoclasts from female mice to enhance coupling to osteoblasts","volume":"154","author":"Ota","year":"2013","journal-title":"Endocrinology"},{"key":"10.1016\/j.isci.2025.113317_bib43","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.isci.2025.113317_bib44","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/978-1-59745-352-3_3","article-title":"Propagation and culture of renal fibroblasts","volume":"466","author":"Grimwood","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.isci.2025.113317_bib45","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.2353\/ajpath.2010.091183","article-title":"Transforming growth factor-{beta}1 induces Smad3-dependent {beta}1 integrin gene expression in epithelial-to-mesenchymal transition during chronic tubulointerstitial fibrosis","volume":"177","author":"Yeh","year":"2010","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.isci.2025.113317_bib46","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1038\/nmeth.1487","article-title":"Mechanical regulation of cell function with geometrically modulated elastomeric substrates","volume":"7","author":"Fu","year":"2010","journal-title":"Nat. Methods"},{"key":"10.1016\/j.isci.2025.113317_bib47","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/nprot.2010.189","article-title":"Assaying stem cell mechanobiology on microfabricated elastomeric substrates with geometrically modulated rigidity","volume":"6","author":"Yang","year":"2011","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.isci.2025.113317_bib48","article-title":"Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells","volume":"162","author":"Sandin","year":"2020","journal-title":"J. Vis. Exp."}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004225015780?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004225015780?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:25:10Z","timestamp":1778901910000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004225015780"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":48,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["S2589004225015780"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2025.113317","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Depletion of tropomyosin 1.6 alleviates TGF-\u03b21-induced fibroblast activation by promoting the \u03b1-SMA-MMP9 matrix-degrading structure","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2025.113317","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"113317"}}