{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:24:43Z","timestamp":1786991083715,"version":"build-2736575974"},"reference-count":82,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI159450"],"award-info":[{"award-number":["AI159450"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007268","name":"Washington University in St. Louis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.isci.2025.113846","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T05:28:33Z","timestamp":1761370113000},"page":"113846","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single-cell transcriptome atlas of adult male and female human hookworm Ancylostoma ceylanicum"],"prefix":"10.1016","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8848-6595","authenticated-orcid":false,"given":"Suman","family":"Bharti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce A.","family":"Rosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9572-3436","authenticated-orcid":false,"given":"Makedonka","family":"Mitreva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2025.113846_bib1","doi-asserted-by":"crossref","DOI":"10.1038\/nrdp.2016.88","article-title":"Hookworm infection","volume":"2","author":"Loukas","year":"2016","journal-title":"Nat. Rev. Dis. Primers"},{"key":"10.1016\/j.isci.2025.113846_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.0020327","article-title":"The end of the line for hookworm? An update on vaccine development","volume":"2","author":"Devaney","year":"2005","journal-title":"PLoS Med."},{"key":"10.1016\/j.isci.2025.113846_bib3","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/0014-4894(78)90078-4","article-title":"Necator americanus and Ancylostoma duodenale: life history parameters and epidemiological implications of two sympatric hookworms of humans","volume":"44","author":"Hoagland","year":"1978","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.isci.2025.113846_bib4","doi-asserted-by":"crossref","first-page":"369","DOI":"10.4269\/ajtmh.1988.39.369","article-title":"Light, long-lasting Necator infection in a volunteer","volume":"39","author":"Beaver","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.isci.2025.113846_bib5","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1056\/NEJMra032492","article-title":"Hookworm infection","volume":"351","author":"Hotez","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.isci.2025.113846_bib6","doi-asserted-by":"crossref","first-page":"25","DOI":"10.4269\/ajtmh.1997.57.25","article-title":"Failure of mebendazole in treatment of human hookworm infections in the southern region of Mali","volume":"57","author":"De Clercq","year":"1997","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.isci.2025.113846_bib7","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1186\/s13071-019-3828-6","article-title":"Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat?","volume":"12","author":"Jimenez Castro","year":"2019","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.isci.2025.113846_bib8","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1038\/ng.2875","article-title":"Genome of the human hookworm Necator americanus","volume":"46","author":"Tang","year":"2014","journal-title":"Nat. Genet."},{"key":"10.1016\/j.isci.2025.113846_bib9","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1038\/ng.3237","article-title":"The genome and transcriptome of the zoonotic hookworm Ancylostoma ceylanicum identify infection-specific gene families","volume":"47","author":"Schwarz","year":"2015","journal-title":"Nat. Genet."},{"key":"10.1016\/j.isci.2025.113846_bib10","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/s41588-018-0262-1","article-title":"Comparative genomics of the major parasitic worms","volume":"51","year":"2019","journal-title":"Nat. Genet."},{"key":"10.1016\/j.isci.2025.113846_bib11","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.ijpara.2020.05.003","article-title":"Transcriptomic analysis of hookworm Ancylostoma ceylanicum life cycle stages reveals changes in G-protein coupled receptor diversity associated with the onset of parasitism","volume":"50","author":"Bernot","year":"2020","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.isci.2025.113846_bib12","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens12010095","article-title":"Excretory\/Secretory Proteome of Females and Males of the Hookworm Ancylostoma ceylanicum","volume":"12","author":"Uzoechi","year":"2023","journal-title":"Pathogens"},{"key":"10.1016\/j.isci.2025.113846_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002678","article-title":"Genome-wide tissue-specific gene expression, co-expression and regulation of co-expressed genes in adult nematode Ascaris suum","volume":"8","author":"Rosa","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.isci.2025.113846_bib14","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.83100","article-title":"Resolving the origins of secretory products and anthelmintic responses in a human parasitic nematode at single-cell resolution","volume":"12","author":"Henthorn","year":"2023","journal-title":"eLife"},{"key":"10.1016\/j.isci.2025.113846_bib15","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1126\/science.aam8940","article-title":"Comprehensive single-cell transcriptional profiling of a multicellular organism","volume":"357","author":"Cao","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.isci.2025.113846_bib16","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adg0506","article-title":"Whole-body gene expression atlas of an adult metazoan","volume":"9","author":"Ghaddar","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.isci.2025.113846_bib17","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/bfgp\/elx010","article-title":"The genomic basis of nematode parasitism","volume":"17","author":"Viney","year":"2018","journal-title":"Brief. Funct. Genomics"},{"key":"10.1016\/j.isci.2025.113846_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.112902","article-title":"Individual cell types in C. elegans age differently and activate distinct cell-protective responses","volume":"42","author":"Roux","year":"2023","journal-title":"Cell Rep."},{"key":"10.1016\/j.isci.2025.113846_bib19","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/s41587-023-01767-y","article-title":"Dictionary learning for integrative, multimodal and scalable single-cell analysis","volume":"42","author":"Hao","year":"2024","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.isci.2025.113846_bib20","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1186\/s13071-016-1795-8","article-title":"The hookworm Ancylostoma ceylanicum intestinal transcriptome provides a platform for selecting drug and vaccine candidates","volume":"9","author":"Wei","year":"2016","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.isci.2025.113846_bib21","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.ebiom.2015.07.030","article-title":"Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential","volume":"2","author":"Wang","year":"2015","journal-title":"EBioMedicine"},{"key":"10.1016\/j.isci.2025.113846_bib22","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.isci.2025.113846_bib23","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.molbiopara.2016.11.005","article-title":"WormBase ParaSite - a comprehensive resource for helminth genomics","volume":"215","author":"Howe","year":"2017","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.isci.2025.113846_bib24","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/nature10553","article-title":"Ascaris suum draft genome","volume":"479","author":"Jex","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.isci.2025.113846_bib25","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1186\/s12864-024-10203-7","article-title":"Intestinal cell diversity and treatment responses in a parasitic nematode at single cell resolution","volume":"25","author":"Tyagi","year":"2024","journal-title":"BMC Genom."},{"key":"10.1016\/j.isci.2025.113846_bib26","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1002\/pro.4193","article-title":"PANNZER-A practical tool for protein function prediction","volume":"31","author":"Toronen","year":"2022","journal-title":"Protein Sci."},{"key":"10.1016\/j.isci.2025.113846_bib27","doi-asserted-by":"crossref","first-page":"1700071","DOI":"10.1002\/pmic.201700071","article-title":"Sma3s: A universal tool for easy functional annotation of proteomes and transcriptomes","volume":"17","author":"Casimiro-Soriguer","year":"2017","journal-title":"Proteomics"},{"key":"10.1016\/j.isci.2025.113846_bib28","doi-asserted-by":"crossref","first-page":"D545","DOI":"10.1093\/nar\/gkaa970","article-title":"KEGG: integrating viruses and cellular organisms","volume":"49","author":"Kanehisa","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2025.113846_bib29","doi-asserted-by":"crossref","first-page":"D344","DOI":"10.1093\/nar\/gkaa977","article-title":"The InterPro protein families and domains database: 20 years on","volume":"49","author":"Blum","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2025.113846_bib30","doi-asserted-by":"crossref","first-page":"D325","DOI":"10.1093\/nar\/gkaa1113","article-title":"The Gene Ontology resource: enriching a GOld mine","volume":"49","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2025.113846_bib31","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/s41587-019-0036-z","article-title":"SignalP 5.0 improves signal peptide predictions using deep neural networks","volume":"37","author":"Almagro Armenteros","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.isci.2025.113846_bib32","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1186\/s13059-019-1832-y","article-title":"OrthoFinder: phylogenetic orthology inference for comparative genomics","volume":"20","author":"Emms","year":"2019","journal-title":"Genome Biol."},{"key":"10.1016\/j.isci.2025.113846_bib33","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1111\/j.1474-9726.2011.00713.x","article-title":"Loss of intestinal nuclei and intestinal integrity in aging C. elegans","volume":"10","author":"McGee","year":"2011","journal-title":"Aging Cell"},{"key":"10.1016\/j.isci.2025.113846_bib34","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.exppara.2017.03.009","article-title":"Tissue distribution and functional analysis of vitellogenin-6 of Toxocara canis","volume":"177","author":"Zhu","year":"2017","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.isci.2025.113846_bib35","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.3389\/fphys.2019.01067","article-title":"Vitellogenins - Yolk Gene Function and Regulation in Caenorhabditis elegans","volume":"10","author":"Perez","year":"2019","journal-title":"Front. Physiol."},{"key":"10.1016\/j.isci.2025.113846_bib36","doi-asserted-by":"crossref","first-page":"35950","DOI":"10.1074\/jbc.M405842200","article-title":"A multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms","volume":"279","author":"Williamson","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2025.113846_bib37","article-title":"A highly expressed intestinal cysteine protease of Ancylostoma ceylanicum protects vaccinated hamsters from hookworm infection","volume":"13","author":"Jason Jason","year":"2019","journal-title":"PLoS Negl. Trop Dis."},{"key":"10.1016\/j.isci.2025.113846_bib38","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0014-5793(97)01441-5","article-title":"Cloning and characterization of a novel human pH-dependent organic cation transporter, OCTN1","volume":"419","author":"Tamai I","year":"1997","journal-title":"FEBS Lett."},{"key":"10.1016\/j.isci.2025.113846_bib39","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/j.ijpara.2008.04.004","article-title":"Analysis of the transthyretin-like (TTL) gene family in Ostertagia ostertagi--comparison with other strongylid nematodes and Caenorhabditis elegans","volume":"38","author":"Saverwyns","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.isci.2025.113846_bib40","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009828","article-title":"Mining nematode protein secretomes to explain lifestyle and host specificity","volume":"15","author":"Tritten","year":"2021","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.isci.2025.113846_bib41","author":"Chonkar"},{"key":"10.1016\/j.isci.2025.113846_bib42","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.molbiopara.2011.01.008","article-title":"Ac-AP-12, a novel factor Xa anticoagulant peptide from the esophageal glands of adult Ancylostoma caninum","volume":"177","author":"Jiang","year":"2011","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.isci.2025.113846_bib43","doi-asserted-by":"crossref","first-page":"683","DOI":"10.3390\/genes11060683","article-title":"Immunoreactive Proteins in the Esophageal Gland Cells of Anisakis Simplex Sensu Stricto Detected by MALDI-TOF\/TOF Analysis","volume":"11","author":"Robertson","year":"2020","journal-title":"Genes"},{"key":"10.1016\/j.isci.2025.113846_bib44","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/S0020-7519(03)00111-5","article-title":"Molecular characterisation of the Ancylostoma-secreted protein family from the adult stage of Ancylostoma caninum","volume":"33","author":"Zhan","year":"2003","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.isci.2025.113846_bib45","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1007300","article-title":"Secreted venom allergen-like proteins of helminths: Conserved modulators of host responses in animals and plants","volume":"14","author":"Wilbers","year":"2018","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.isci.2025.113846_bib46","doi-asserted-by":"crossref","first-page":"238","DOI":"10.4269\/ajtmh.2002.66.238","article-title":"Molecular cloning and purification of Ac-TMP, a developmentally regulated putative tissue inhibitor of metalloprotease released in relative abundance by adult Ancylostoma hookworms","volume":"66","author":"Zhan","year":"2002","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.isci.2025.113846_bib47","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ygeno.2018.03.007","article-title":"Genome-wide identification and comprehensive analysis of Excretory\/Secretory proteins in nematodes provide potential drug targets for parasite control","volume":"111","author":"Gahoi","year":"2019","journal-title":"Genomics"},{"key":"10.1016\/j.isci.2025.113846_bib48","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/bs.apar.2024.03.001","article-title":"Astacin metalloproteases in human-parasitic nematodes","volume":"126","author":"Moser","year":"2024","journal-title":"Adv. Parasitol."},{"key":"10.1016\/j.isci.2025.113846_bib49","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1038\/nn.3253","article-title":"PDF-1 neuropeptide signaling modulates a neural circuit for mate-searching behavior in C. elegans","volume":"15","author":"Barrios","year":"2012","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.isci.2025.113846_bib50","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/s41467-020-20269-y","article-title":"Steroid hormones sulfatase inactivation extends lifespan and ameliorates age-related diseases","volume":"12","author":"Perez-Jimenez","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2025.113846_bib51","doi-asserted-by":"crossref","first-page":"666","DOI":"10.4269\/ajtmh.16-0519","article-title":"Characterizing Reactivity to Onchocerca volvulus Antigens in Multiplex Bead Assays","volume":"97","author":"Feeser","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.isci.2025.113846_bib52","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.neuron.2018.07.042","article-title":"The CeNGEN Project: The Complete Gene Expression Map of an Entire Nervous System","volume":"99","author":"Hammarlund","year":"2018","journal-title":"Neuron"},{"key":"10.1016\/j.isci.2025.113846_bib53","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1091\/mbc.e04-03-0179","article-title":"Tropomyosin and troponin are required for ovarian contraction in the Caenorhabditis elegans reproductive system","volume":"15","author":"Ono","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.isci.2025.113846_bib54","first-page":"1","article-title":"Egg-laying","volume":"14","author":"Schafer","year":"2005","journal-title":"WormBook"},{"key":"10.1016\/j.isci.2025.113846_bib55","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/cm.21281","article-title":"Molecular evolution of troponin I and a role of its N-terminal extension in nematode locomotion","volume":"73","author":"Barnes","year":"2016","journal-title":"Cytoskeleton (Hoboken)"},{"key":"10.1016\/j.isci.2025.113846_bib56","first-page":"1","article-title":"Development, structure, and maintenance of C. elegans body wall muscle","volume":"13","author":"Gieseler","year":"2017"},{"key":"10.1016\/j.isci.2025.113846_bib57","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1534\/g3.113.007914","article-title":"When females produce sperm: genetics of C. elegans hermaphrodite reproductive choice","volume":"3","author":"Bahrami","year":"2013","journal-title":"G3 (Bethesda)"},{"key":"10.1016\/j.isci.2025.113846_bib58","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1007\/s12639-015-0707-8","article-title":"Nematodes ultrastructure: complex systems and processes","volume":"40","author":"Basyoni","year":"2016","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.isci.2025.113846_bib59","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1083\/jcb.102.6.2033","article-title":"Intermediate filaments in muscle and epithelial cells of nematodes","volume":"102","author":"Bartnik","year":"1986","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.113846_bib60","author":"Chonkar","year":"2018","journal-title":"Old World Hookworm Parasitology"},{"key":"10.1016\/j.isci.2025.113846_bib61","series-title":"Encyclopedia of Parasitology","first-page":"950","article-title":"Nematodes","author":"Mehlhorn","year":"2008"},{"key":"10.1016\/j.isci.2025.113846_bib62","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.ydbio.2018.08.005","article-title":"CBD-1 organizes two independent complexes required for eggshell vitelline layer formation and egg activation in C. elegans","volume":"442","author":"Gonzalez","year":"2018","journal-title":"Dev. Biol."},{"key":"10.1016\/j.isci.2025.113846_bib63","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1083\/jcb.200603003","article-title":"Identification of novel chondroitin proteoglycans in Caenorhabditis elegans: embryonic cell division depends on CPG-1 and CPG-2","volume":"173","author":"Olson","year":"2006","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2025.113846_bib64","first-page":"1","article-title":"Sperm motility and MSP","volume":"1","author":"Smith","year":"2006"},{"key":"10.1016\/j.isci.2025.113846_bib65","doi-asserted-by":"crossref","first-page":"3588","DOI":"10.1073\/pnas.0911685107","article-title":"Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans","volume":"107","author":"Conine","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2025.113846_bib66","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1002\/dvdy.22271","article-title":"Spermatogenesis-defective (spe) mutants of the nematode Caenorhabditis elegans provide clues to solve the puzzle of male germline functions during reproduction","volume":"239","author":"Nishimura","year":"2010","journal-title":"Dev. Dyn."},{"key":"10.1016\/j.isci.2025.113846_bib67","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.cub.2005.12.041","article-title":"Regulation of sperm activation by SWM-1 is required for reproductive success of C. elegans males","volume":"16","author":"Stanfield","year":"2006","journal-title":"Curr. Biol."},{"key":"10.1016\/j.isci.2025.113846_bib68","doi-asserted-by":"crossref","DOI":"10.3390\/biom13040657","article-title":"Spermiogenesis in Caenorhabditis elegans: An Excellent Model to Explore the Molecular Basis for Sperm Activation","volume":"13","author":"Shimada","year":"2023","journal-title":"Biomolecules"},{"key":"10.1016\/j.isci.2025.113846_bib69","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1094\/PHYTO-04-22-0127-R","article-title":"Two Novel Bursaphelenchus xylophilus Kunitz Effector Proteins Using Different Infection and Survival Strategies to Suppress Immunity in Pine","volume":"113","author":"Wen","year":"2023","journal-title":"Phytopathology"},{"key":"10.1016\/j.isci.2025.113846_bib70","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1093\/molbev\/msaa207","article-title":"Spatial Transcriptomics of Nematodes Identifies Sperm Cells as a Source of Genomic Novelty and Rapid Evolution","volume":"38","author":"Rodelsperger","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.isci.2025.113846_bib71","series-title":"WormAtlas","article-title":"Male neuronal support cells, spicules","author":"Lints","year":"2013"},{"key":"10.1016\/j.isci.2025.113846_bib72","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cels.2019.03.003","article-title":"DoubletFinder: Doublet Detection in Single-Cell RNA Sequencing Data Using Artificial Nearest Neighbors","volume":"8","author":"McGinnis","year":"2019","journal-title":"Cell Syst."},{"key":"10.1016\/j.isci.2025.113846_bib73","doi-asserted-by":"crossref","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","article-title":"Integrated analysis of multimodal single-cell data","volume":"184","author":"Hao","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.isci.2025.113846_bib74","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1186\/s13059-015-0844-5","article-title":"MAST: a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data","volume":"16","author":"Finak","year":"2015","journal-title":"Genome Biol."},{"key":"10.1016\/j.isci.2025.113846_bib75","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","article-title":"InterProScan 5: genome-scale protein function classification","volume":"30","author":"Jones","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.isci.2025.113846_bib76","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.jmb.2015.11.006","article-title":"BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences","volume":"428","author":"Kanehisa","year":"2016","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.isci.2025.113846_bib81","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gku1128","article-title":"Helminth.net: expansions to Nematode.net and an introduction to Trematode.net","volume":"43","author":"Martin","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.isci.2025.113846_bib82","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/978-1-4939-7737-6_13","article-title":"A Multi-Omics Database for Parasitic Nematodes and Trematodes","volume":"1757","author":"Martin","year":"2018","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.isci.2025.113846_bib77","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0008237","article-title":"Comprehensive analysis of the secreted proteome of adult Necator americanus hookworms","volume":"14","author":"Logan","year":"2020","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.isci.2025.113846_bib78","doi-asserted-by":"crossref","first-page":"D884","DOI":"10.1093\/nar\/gkaa942","article-title":"Ensembl 2021","volume":"49","author":"Howe","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2025.113846_bib79","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2023.102603","article-title":"Optimized protocol for whole-mount RNA fluorescent in situ hybridization using oxidation-mediated autofluorescence reduction on mouse embryos","volume":"4","author":"Morabito","year":"2023","journal-title":"STAR Protoc."},{"key":"10.1016\/j.isci.2025.113846_bib80","doi-asserted-by":"crossref","first-page":"3632","DOI":"10.1242\/dev.140137","article-title":"Mapping a multiplexed zoo of mRNA expression","volume":"143","author":"Choi","year":"2016","journal-title":"Development"}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004225021078?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004225021078?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:41:28Z","timestamp":1782866488000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004225021078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":82,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["S2589004225021078"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2025.113846","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.01.03.631133","asserted-by":"object"}]},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-cell transcriptome atlas of adult male and female human hookworm Ancylostoma ceylanicum","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2025.113846","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"113846"}}