{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T01:52:24Z","timestamp":1781833944527,"version":"3.54.5"},"reference-count":37,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007343","name":"Shire","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007343","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["18\/CRT\/6214"],"award-info":[{"award-number":["18\/CRT\/6214"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007495","name":"Fondation Thierry Latran","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007495","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000406","name":"Motor Neurone Disease Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000406","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014076","name":"Stichting ALS Nederland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014012","name":"FightMND","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014012","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100025294","name":"Research Ireland","doi-asserted-by":"publisher","award":["21\/RC\/10294_P2"],"award-info":[{"award-number":["21\/RC\/10294_P2"]}],"id":[{"id":"10.13039\/501100025294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100025294","name":"Research Ireland","doi-asserted-by":"publisher","award":["20\/SP\/8953"],"award-info":[{"award-number":["20\/SP\/8953"]}],"id":[{"id":"10.13039\/501100025294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100025294","name":"Research Ireland","doi-asserted-by":"publisher","award":["17\/JPND\/3455"],"award-info":[{"award-number":["17\/JPND\/3455"]}],"id":[{"id":"10.13039\/501100025294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012921","name":"Royal College of Surgeons in Ireland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012921","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"Taighde Eireann - Research Ireland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.isci.2026.115636","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T16:32:25Z","timestamp":1775579545000},"page":"115636","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Small RNA sequencing identifies serum tDR-1:34-Gly-GCC tiRNA levels as a biomarker for survival in amyotrophic lateral sclerosis"],"prefix":"10.1016","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9606-0531","authenticated-orcid":false,"given":"Koen C.","family":"Demaegd","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindy","family":"Kool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharada","family":"Baindoor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Donovan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyi","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henk-Jan","family":"Westeneng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben P.A.","family":"van Eijk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grainne","family":"Geoghegan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Perez Morrissey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luise","family":"Halang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koen","family":"Poesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pegah","family":"Masrori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hesham A.Y.","family":"Gibriel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Jirstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten T.","family":"Ven\u00f8","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rgen","family":"Kjems","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orla","family":"Hardiman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan H.","family":"Veldink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Kenna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard H.","family":"van den Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Van Damme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen H.M.","family":"Prehn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"van Es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2026.115636_bib1","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1016\/S0140-6736(17)31287-4","article-title":"Amyotrophic lateral sclerosis","volume":"390","author":"van Es","year":"2017","journal-title":"Lancet"},{"key":"10.1016\/j.isci.2026.115636_bib2","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1016\/j.mayocp.2018.09.009","article-title":"Rethinking Amyotrophic Lateral Sclerosis","volume":"93","author":"Rosenfeld","year":"2018","journal-title":"Mayo Clin. Proc."},{"key":"10.1016\/j.isci.2026.115636_bib3","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1002\/mus.24198","article-title":"Genetic heterogeneity of amyotrophic lateral sclerosis: implications for clinical practice and research","volume":"49","author":"Su","year":"2014","journal-title":"Muscle Nerve"},{"key":"10.1016\/j.isci.2026.115636_bib4","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1080\/21678421.2016.1267768","article-title":"Amyotrophic lateral sclerosis - frontotemporal spectrum disorder (ALS-FTSD): Revised diagnostic criteria","volume":"18","author":"Strong","year":"2017","journal-title":"Amyotroph. Lateral Scler. Frontotemporal Degener."},{"key":"10.1016\/j.isci.2026.115636_bib5","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1212\/WNL.0000000000012545","article-title":"Innovating Clinical Trials for Amyotrophic Lateral Sclerosis: Challenging the Established Order","volume":"97","author":"van Eijk","year":"2021","journal-title":"Neurology"},{"key":"10.1016\/j.isci.2026.115636_bib6","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/S1474-4422(18)30089-9","article-title":"Prognosis for patients with amyotrophic lateral sclerosis: development and validation of a personalised prediction model","volume":"17","author":"Westeneng","year":"2018","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.isci.2026.115636_bib7","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1093\/brain\/awac394","article-title":"Neurofilament light chain in drug development for amyotrophic lateral sclerosis: a critical appraisal","volume":"146","author":"Benatar","year":"2023","journal-title":"Brain"},{"key":"10.1016\/j.isci.2026.115636_bib8","doi-asserted-by":"crossref","first-page":"364","DOI":"10.3389\/fgene.2019.00364","article-title":"Small Non-coding RNAs: New Class of Biomarkers and Potential Therapeutic Targets in Neurodegenerative Disease","volume":"10","author":"Watson","year":"2019","journal-title":"Front. Genet."},{"key":"10.1016\/j.isci.2026.115636_bib9","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1038\/s41593-021-00936-z","article-title":"Circulating miR-181 is a prognostic biomarker for amyotrophic lateral sclerosis","volume":"24","author":"Magen","year":"2021","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.isci.2026.115636_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2024.105323","article-title":"Prognostic clinical and biological markers for amyotrophic lateral sclerosis disease progression: validation and implications for clinical trial design and analysis","volume":"108","author":"Benatar","year":"2024","journal-title":"EBioMedicine"},{"key":"10.1016\/j.isci.2026.115636_bib11","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1101\/gad.1837609","article-title":"A novel class of small RNAs: tRNA-derived RNA fragments (tRFs)","volume":"23","author":"Lee","year":"2009","journal-title":"Genes Dev."},{"key":"10.1016\/j.isci.2026.115636_bib12","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.febslet.2008.12.043","article-title":"Stress induces tRNA cleavage by angiogenin in mammalian cells","volume":"583","author":"Fu","year":"2009","journal-title":"FEBS Lett."},{"key":"10.1016\/j.isci.2026.115636_bib13","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1083\/jcb.200811106","article-title":"Angiogenin cleaves tRNA and promotes stress-induced translational repression","volume":"185","author":"Yamasaki","year":"2009","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2026.115636_bib14","doi-asserted-by":"crossref","first-page":"18201","DOI":"10.1073\/pnas.1407361111","article-title":"G-quadruplex structures contribute to the neuroprotective effects of angiogenin-induced tRNA fragments","volume":"111","author":"Ivanov","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2026.115636_bib15","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1261\/rna.065516.117","article-title":"Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor","volume":"24","author":"Thomas","year":"2018","journal-title":"RNA"},{"key":"10.1016\/j.isci.2026.115636_bib16","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1016\/S1474-4422(14)70219-4","article-title":"Analysis of amyotrophic lateral sclerosis as a multistep process: a population-based modelling study","volume":"13","author":"Al-Chalabi","year":"2014","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.isci.2026.115636_bib17","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1083\/jcb.201609081","article-title":"Mechanistic insights into mammalian stress granule dynamics","volume":"215","author":"Panas","year":"2016","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2026.115636_bib18","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/978-3-319-89689-2_7","article-title":"Stress Granules and ALS: A Case of Causation or Correlation?","volume":"20","author":"Fernandes","year":"2018","journal-title":"Adv. Neurobiol."},{"key":"10.1016\/j.isci.2026.115636_bib19","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/1750-1326-7-53","article-title":"Expression of Fused in sarcoma mutations in mice recapitulates the neuropathology of FUS proteinopathies and provides insight into disease pathogenesis","volume":"7","author":"Verbeeck","year":"2012","journal-title":"Mol. Neurodegener."},{"key":"10.1016\/j.isci.2026.115636_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.expneurol.2024.115128","article-title":"Effects of ALS-associated 5'tiRNA(Gly-GCC) on the transcriptomic and proteomic profile of primary neurons in vitro","volume":"385","author":"Jirstr\u00f6m","year":"2025","journal-title":"Exp. Neurol."},{"key":"10.1016\/j.isci.2026.115636_bib21","doi-asserted-by":"crossref","DOI":"10.1093\/braincomms\/fcaa138","article-title":"5'ValCAC tRNA fragment generated as part of a protective angiogenin response provides prognostic value in amyotrophic lateral sclerosis","volume":"2","author":"Hogg","year":"2020","journal-title":"Brain Commun."},{"key":"10.1016\/j.isci.2026.115636_bib22","doi-asserted-by":"crossref","DOI":"10.1093\/braincomms\/fcaa053","article-title":"Identification of a potential non-coding RNA biomarker signature for amyotrophic lateral sclerosis","volume":"2","author":"Joilin","year":"2020","journal-title":"Brain Commun."},{"key":"10.1016\/j.isci.2026.115636_bib23","series-title":"Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration","first-page":"1","article-title":"Serum small non-coding RNA define molecular subtypes in amyotrophic lateral sclerosis","author":"Baindoor","year":"2025"},{"key":"10.1016\/j.isci.2026.115636_bib24","doi-asserted-by":"crossref","DOI":"10.1242\/dmm.050870","article-title":"Distinct fingerprints of tRNA-derived small non-coding RNA in animal models of neurodegeneration","volume":"17","author":"Baindoor","year":"2024","journal-title":"Dis. Model. Mech."},{"key":"10.1016\/j.isci.2026.115636_bib25","doi-asserted-by":"crossref","DOI":"10.1111\/bpa.13328","article-title":"Protective role of Angiogenin in muscle regeneration in amyotrophic lateral sclerosis: Diagnostic and therapeutic implications","volume":"35","author":"Fabbrizio","year":"2025","journal-title":"Brain Pathol."},{"key":"10.1016\/j.isci.2026.115636_bib26","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1002\/jcsm.13187","article-title":"tRNA-derived small RNA, 5'tiRNA-Gly-CCC, promotes skeletal muscle regeneration through the inflammatory response","volume":"14","author":"Shen","year":"2023","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"10.1016\/j.isci.2026.115636_bib27","doi-asserted-by":"crossref","first-page":"186","DOI":"10.3389\/fneur.2019.00186","article-title":"An Overview of MicroRNAs as Biomarkers of ALS","volume":"10","author":"Joilin","year":"2019","journal-title":"Front. Neurol."},{"key":"10.1016\/j.isci.2026.115636_bib28","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1136\/jnnp.2011.244939","article-title":"Population based epidemiology of amyotrophic lateral sclerosis using capture-recapture methodology","volume":"82","author":"Huisman","year":"2011","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"10.1016\/j.isci.2026.115636_bib29","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1136\/practneurol-2021-003256","article-title":"Diagnosing ALS: the Gold Coast criteria and the role of EMG","volume":"22","author":"Turner","year":"2022","journal-title":"Pract. Neurol."},{"key":"10.1016\/j.isci.2026.115636_bib30","doi-asserted-by":"crossref","first-page":"4424","DOI":"10.1093\/bioinformatics\/btab515","article-title":"tsRNAsearch: a pipeline for the identification of tRNA and ncRNA fragments from small RNA-sequencing data","volume":"37","author":"Donovan","year":"2021","journal-title":"Bioinformatics"},{"key":"10.1016\/j.isci.2026.115636_bib31","series-title":"FelixKrueger\/TrimGalore: v0.6.10 - Add Default Decompression Path (0.6.10)","author":"Felix Krueger","year":"2023"},{"key":"10.1016\/j.isci.2026.115636_bib32","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.isci.2026.115636_bib33","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.isci.2026.115636_bib34","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.isci.2026.115636_bib35","author":"Leek"},{"key":"10.1016\/j.isci.2026.115636_bib36","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. Roy. Stat. Soc. B Stat. Methodol."},{"key":"10.1016\/j.isci.2026.115636_bib37","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1212\/01.wnl.0000244466.46020.47","article-title":"Elevated serum angiogenin levels in ALS","volume":"67","author":"Cronin","year":"2006","journal-title":"Neurology"}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226010114?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226010114?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T01:32:50Z","timestamp":1781832770000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004226010114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["S2589004226010114"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2026.115636","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Small RNA sequencing identifies serum tDR-1:34-Gly-GCC tiRNA levels as a biomarker for survival in amyotrophic lateral sclerosis","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2026.115636","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"115636"}}