{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:45:30Z","timestamp":1791841530210,"version":"4.3.4"},"reference-count":101,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs Program","doi-asserted-by":"publisher","award":["CRC-2024-00304"],"award-info":[{"award-number":["CRC-2024-00304"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000106","name":"Saskatchewan Health Research Foundation","doi-asserted-by":"publisher","award":["6115"],"award-info":[{"award-number":["6115"]}],"id":[{"id":"10.13039\/501100000106","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["DG 2024-06684"],"award-info":[{"award-number":["DG 2024-06684"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008580","name":"University of Regina","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008580","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["202403PPE-522800"],"award-info":[{"award-number":["202403PPE-522800"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100029584","name":"Consortium for Graduate Study in Management","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100029584","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.isci.2026.116145","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T15:34:01Z","timestamp":1780328041000},"page":"116145","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Uncovering the mechanisms of clinically relevant altered antibiotic responses of Staphylococcus aureus under wound infection-mimetic conditions"],"prefix":"10.1016","volume":"29","author":[{"given":"Colin D.","family":"Rieger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Molaeitabari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanya E.S.","family":"Dahms","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar M.","family":"El-Halfawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2026.116145_bib1","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1016\/S1473-3099(13)70318-9","article-title":"Antibiotic resistance-the need for global solutions","volume":"13","author":"Laxminarayan","year":"2013","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.isci.2026.116145_bib2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.mib.2020.02.003","article-title":"The social life of microbes in chronic infection","volume":"53","author":"Ibberson","year":"2020","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib3","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1046\/j.1524-475X.1994.20305.x","article-title":"Definitions and guidelines for assessment of wounds and evaluation of healing","volume":"2","author":"Lazarus","year":"1994","journal-title":"Wound Repair Regen."},{"key":"10.1016\/j.isci.2026.116145_bib4","series-title":"Mechanisms of Vascular Disease: A Reference Book for Vascular Specialists","article-title":"Principles of Wound Healing","author":"Schultz","year":"2011"},{"key":"10.1016\/j.isci.2026.116145_bib5","doi-asserted-by":"crossref","first-page":"4","DOI":"10.12968\/bjcn.2003.8.Sup3.11577","article-title":"Wound exudate: composition and functions","volume":"8","author":"Cutting","year":"2003","journal-title":"Br. J. Community Nurs."},{"key":"10.1016\/j.isci.2026.116145_bib6","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/s41572-022-00377-3","article-title":"Chronic wounds","volume":"8","author":"Falanga","year":"2022","journal-title":"Nat. Rev. Dis. Primers"},{"key":"10.1016\/j.isci.2026.116145_bib7","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1111\/iwj.14491","article-title":"What's the true costs of wounds faced by different healthcare systems around the world?","volume":"20","author":"Queen","year":"2023","journal-title":"Int. Wound J."},{"key":"10.1016\/j.isci.2026.116145_bib8","doi-asserted-by":"crossref","first-page":"e14709","DOI":"10.1111\/iwj.14709","article-title":"Estimating the cost of wounds both nationally and regionally within the top 10 highest spenders","volume":"21","author":"Queen","year":"2024","journal-title":"Int. Wound J."},{"key":"10.1016\/j.isci.2026.116145_bib9","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1007\/s00248-016-0867-9","article-title":"Bacterial Contribution in Chronicity of Wounds","volume":"73","author":"Rahim","year":"2017","journal-title":"Microb. Ecol."},{"key":"10.1016\/j.isci.2026.116145_bib10","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"The lancet"},{"key":"10.1016\/j.isci.2026.116145_bib11","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/CMR.00134-14","article-title":"Staphylococcus aureus Infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management","volume":"28","author":"Tong","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.isci.2026.116145_bib12","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1089\/sur.2013.134","article-title":"Infection in Burns","volume":"17","author":"Norbury","year":"2016","journal-title":"Surg. Infect."},{"key":"10.1016\/j.isci.2026.116145_bib13","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1742-481X.2006.00159.x","article-title":"Multiple bacterial species reside in chronic wounds: a longitudinal study","volume":"3","author":"Gj\u00f8dsb\u00f8l","year":"2006","journal-title":"Int. Wound J."},{"key":"10.1016\/j.isci.2026.116145_bib14","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.3201\/eid1807.101371","article-title":"Predicting risk for death from MRSA bacteremia","volume":"18","author":"Pastagia","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.isci.2026.116145_bib15","series-title":"Prioritization of Pathogens to Guide Discovery, Research and Development of New Antibiotics for Drug-Resistant Bacterial Infections Including Tuberculosis","year":"2017"},{"key":"10.1016\/j.isci.2026.116145_bib16","doi-asserted-by":"crossref","first-page":"iv55","DOI":"10.1093\/jac\/dkz288","article-title":"Characterization of MRSA in Canada from 2007 to 2016","volume":"74","author":"Nichol","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.isci.2026.116145_bib17","doi-asserted-by":"crossref","first-page":"330","DOI":"10.3181\/00379727-48-13311","article-title":"A protein-free medium for primary isolation of the Gonococcus and Meningococcus","volume":"48","author":"Mueller","year":"1941","journal-title":"Exp. Biol. Med. (Maywood)."},{"key":"10.1016\/j.isci.2026.116145_bib18","series-title":"Performance Standards for Antimicrobial Susceptibility Testing","year":"2017"},{"key":"10.1016\/j.isci.2026.116145_bib19","doi-asserted-by":"crossref","first-page":"ii105","DOI":"10.1093\/jac\/dkn357","article-title":"Clinical implications of antimicrobial resistance for therapy","volume":"62","author":"Macgowan","year":"2008","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.isci.2026.116145_bib20","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.jcf.2012.02.006","article-title":"Results of antibiotic susceptibility testing do not influence clinical outcome in children with cystic fibrosis","volume":"11","author":"Hurley","year":"2012","journal-title":"J. Cyst. Fibros."},{"key":"10.1016\/j.isci.2026.116145_bib21","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1378\/chest.123.5.1495","article-title":"Susceptibility testing of Pseudomonas aeruginosa isolates and clinical response to parenteral antibiotic administration: lack of association in cystic fibrosis","volume":"123","author":"Smith","year":"2003","journal-title":"Chest"},{"key":"10.1016\/j.isci.2026.116145_bib22","doi-asserted-by":"crossref","first-page":"e02259-20","DOI":"10.1128\/mBio.02259-20","article-title":"Gene Dispensability in Escherichia coli Grown in Thirty Different Carbon Environments","volume":"11","author":"Tong","year":"2020","journal-title":"mBio"},{"key":"10.1016\/j.isci.2026.116145_bib23","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/nchembio.1361","article-title":"Metabolic suppression identifies new antibacterial inhibitors under nutrient limitation","volume":"9","author":"Zlitni","year":"2013","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.isci.2026.116145_bib24","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1097\/01.BCR.0000112331.72232.1B","article-title":"Microbicidal properties of a silver-containing hydrofiber dressing against a variety of burn wound pathogens","volume":"25","author":"Bowler","year":"2004","journal-title":"J. Burn Care Rehabil."},{"key":"10.1016\/j.isci.2026.116145_bib25","article-title":"Wound exudate and the role of dressings. A consensus document","volume":"5","year":"2008","journal-title":"Int. Wound J."},{"key":"10.1016\/j.isci.2026.116145_bib26","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1111\/j.1524-475X.2007.00259.x","article-title":"Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture","volume":"15","author":"Aiba-Kojima","year":"2007","journal-title":"Wound Repair Regen."},{"key":"10.1016\/j.isci.2026.116145_bib27","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1524-475X.2009.00564.x","article-title":"Do nutritional markers in wound fluid reflect pressure ulcer status?","volume":"18","author":"Iizaka","year":"2010","journal-title":"Wound Repair Regen."},{"key":"10.1016\/j.isci.2026.116145_bib28","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1046\/j.1524-475x.2000.00264.x","article-title":"Simple biochemical markers to assess chronic wounds","volume":"8","author":"James","year":"2000","journal-title":"Wound Repair Regen."},{"key":"10.1016\/j.isci.2026.116145_bib29","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1046\/j.1524-475X.1996.40211.x","article-title":"Biochemical analysis of wound fluid from nonhealing and healing chronic leg ulcers","volume":"4","author":"Trengove","year":"1996","journal-title":"Wound Repair Regen."},{"key":"10.1016\/j.isci.2026.116145_bib30","doi-asserted-by":"crossref","first-page":"775","DOI":"10.5851\/kosfa.2022.e46","article-title":"Review of the Current Research on Fetal Bovine Serum and the Development of Cultured Meat","volume":"42","author":"Lee","year":"2022","journal-title":"Food Sci. Anim. Resour."},{"key":"10.1016\/j.isci.2026.116145_bib31","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1186\/s12866-019-1682-5","article-title":"An in vitro collagen perfusion wound biofilm model; with applications for antimicrobial studies and microbial metabolomics","volume":"19","author":"Slade","year":"2019","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib32","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1002\/jmor.1051570206","article-title":"A histological study of wound healing and hemocyte function in the wax-moth Galleria mellonella","volume":"157","author":"Rowley","year":"1978","journal-title":"J. Morphol."},{"key":"10.1016\/j.isci.2026.116145_bib33","doi-asserted-by":"crossref","first-page":"998","DOI":"10.3389\/fmicb.2020.00998","article-title":"An Invertebrate Burn Wound Model That Recapitulates the Hallmarks of Burn Trauma and Infection Seen in Mammalian Models","volume":"11","author":"Maslova","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib34","series-title":"Performance Standards for Antimicrobial Susceptibility Testing CLSI Supplement M100. Approved Standard, Pennsylvania","year":"2023"},{"key":"10.1016\/j.isci.2026.116145_bib35","series-title":"Performance Standards for Antimicrobial Susceptibility Testing CLSI Supplement M100. Approved Standard, Pennsylvania","year":"2009"},{"key":"10.1016\/j.isci.2026.116145_bib36","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1749-6632.2011.06354.x","article-title":"The history of the tetracyclines","volume":"1241","author":"Nelson","year":"2011","journal-title":"Ann Ny Acad Sci"},{"key":"10.1016\/j.isci.2026.116145_bib37","doi-asserted-by":"crossref","first-page":"dlaa114","DOI":"10.1093\/jacamr\/dlaa114","article-title":"Treatment of methicillin-resistant Staphylococcus aureus (MRSA): updated guidelines from the UK","volume":"3","author":"Brown","year":"2021","journal-title":"JAC. Antimicrob. Resist."},{"key":"10.1016\/j.isci.2026.116145_bib38","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1021\/cb300413m","article-title":"Inhibition of WTA Synthesis Blocks the Cooperative Action of PBPs and Sensitizes MRSA to beta-Lactams","volume":"8","author":"Farha","year":"2013","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.isci.2026.116145_bib39","doi-asserted-by":"crossref","first-page":"1904","DOI":"10.1128\/JCM.02500-06","article-title":"Comparative genomics of Canadian epidemic lineages of methicillin-resistant Staphylococcus aureus","volume":"45","author":"Christianson","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib40","doi-asserted-by":"crossref","first-page":"e00537-12","DOI":"10.1128\/mBio.00537-12","article-title":"A Genetic Resource for Rapid and Comprehensive Phenotype Screening of Nonessential Staphylococcus aureus Genes","volume":"4","author":"Fey","year":"2013","journal-title":"mBio"},{"key":"10.1016\/j.isci.2026.116145_bib41","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1046\/j.1365-2958.2002.02944.x","article-title":"MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake","volume":"44","author":"Horsburgh","year":"2002","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib42","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1080\/10739149.2015.1105817","article-title":"Characterization of the interaction between bovine serum albumin and doxycycline by chemiluminescence and molecular docking","volume":"44","author":"Tan","year":"2016","journal-title":"Instrumentation Science & Technology"},{"key":"10.1016\/j.isci.2026.116145_bib43","first-page":"e4392","article-title":"The insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis","volume":"e4392","author":"Ramarao","year":"2012","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.isci.2026.116145_bib44","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/978-1-4419-5638-5_2","article-title":"Of model hosts and man: using Caenorhabditis elegans, Drosophila melanogaster and Galleria mellonella as model hosts for infectious disease research","volume":"710","author":"Glavis-Bloom","year":"2012","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.isci.2026.116145_bib45","doi-asserted-by":"crossref","first-page":"RP87026","DOI":"10.7554\/eLife.87026.3","article-title":"Extensive re-modelling of the cell wall during the development of Staphylococcus aureus bacteraemia","volume":"12","author":"Douglas","year":"2023","journal-title":"eLife"},{"key":"10.1016\/j.isci.2026.116145_bib46","doi-asserted-by":"crossref","first-page":"e02070-15","DOI":"10.1128\/mBio.02070-15","article-title":"The Staphylococcus aureus Methicillin Resistance Factor FmtA Is a d-Amino Esterase That Acts on Teichoic Acids","volume":"7","author":"Rahman","year":"2016","journal-title":"mBio"},{"key":"10.1016\/j.isci.2026.116145_bib47","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1128\/jb.158.2.513-516.1984","article-title":"Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus","volume":"158","author":"Hartman","year":"1984","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.isci.2026.116145_bib48","doi-asserted-by":"crossref","first-page":"e1004891","DOI":"10.1371\/journal.ppat.1004891","article-title":"Staphylococcus aureus Survives with a Minimal Peptidoglycan Synthesis Machine but Sacrifices Virulence and Antibiotic Resistance","volume":"11","author":"Reed","year":"2015","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.isci.2026.116145_bib49","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1128\/AAC.05054-11","article-title":"GraXSR Proteins Interact with the VraFG ABC Transporter To Form a Five-Component System Required for Cationic Antimicrobial Peptide Sensing and Resistance in Staphylococcus aureus","volume":"56","author":"Falord","year":"2012","journal-title":"Antimicrob Agents Ch"},{"key":"10.1016\/j.isci.2026.116145_bib50","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/j.jmii.2025.05.007","article-title":"A novel \u03c3B regulatory module in Staphylococcus aureus: Unraveling the multifaceted roles of RsbU domains in stress response mechanisms","volume":"58","author":"Huang","year":"2025","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.isci.2026.116145_bib51","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/j.ijmm.2017.11.011","article-title":"AureoWiki- The repository of the Staphylococcus aureus research and annotation community","volume":"308","author":"Fuchs","year":"2018","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib52","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1128\/AAC.02070-15","article-title":"Effects of Low-Dose Amoxicillin on Staphylococcus aureus USA300 Biofilms","volume":"60","author":"Mlynek","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.isci.2026.116145_bib53","doi-asserted-by":"crossref","first-page":"12510","DOI":"10.1073\/pnas.1404099111","article-title":"Compound-gene interaction mapping reveals distinct roles for Staphylococcus aureus teichoic acids","volume":"111","author":"Maria","year":"2014","journal-title":"P Natl Acad Sci USA"},{"key":"10.1016\/j.isci.2026.116145_bib54","doi-asserted-by":"crossref","first-page":"18033","DOI":"10.1038\/nrdp.2018.33","article-title":"Methicillin-resistant Staphylococcus aureus","volume":"4","author":"Lee","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"10.1016\/j.isci.2026.116145_bib55","doi-asserted-by":"crossref","first-page":"e02727-16","DOI":"10.1128\/AAC.02727-16","article-title":"High-Level Resistance of Staphylococcus aureus to beta-Lactam Antibiotics Mediated by Penicillin-Binding Protein 4 (PBP4)","volume":"61","author":"Hamilton","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.isci.2026.116145_bib56","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/ja.2013.100","article-title":"Taking aim at wall teichoic acid synthesis: new biology and new leads for antibiotics","volume":"67","author":"Sewell","year":"2014","journal-title":"J. Antibiot."},{"key":"10.1016\/j.isci.2026.116145_bib57","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1021\/cb100269f","article-title":"Synthetic Lethal Compound Combinations Reveal a Fundamental Connection between Wall Teichoic Acid and Peptidoglycan Biosyntheses in Staphylococcus aureus","volume":"6","author":"Campbell","year":"2011","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.isci.2026.116145_bib58","doi-asserted-by":"crossref","first-page":"4030","DOI":"10.1128\/JB.00611-08","article-title":"The N-Acetylmannosamine Transferase Catalyzes the First Committed Step of Teichoic Acid Assembly in Bacillus subtilis and Staphylococcus aureus","volume":"191","author":"D'Elia","year":"2009","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.isci.2026.116145_bib59","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1016\/j.bmcl.2010.01.036","article-title":"Development of improved inhibitors of wall teichoic acid biosynthesis with potent activity against Staphylococcus aureus","volume":"20","author":"Lee","year":"2010","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.isci.2026.116145_bib60","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/s41589-019-0401-8","article-title":"Discovery of an antivirulence compound that reverses beta-lactam resistance in MRSA","volume":"16","author":"El-Halfawy","year":"2020","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.isci.2026.116145_bib61","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1038\/s41467-023-37310-5","article-title":"Modulation of MRSA virulence gene expression by the wall teichoic acid enzyme TarO","volume":"14","author":"Lu","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.116145_bib62","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1128\/AAC.41.11.2355","article-title":"Cloning and characterization of the fmt gene which affects the methicillin resistance level and autolysis in the presence of triton X-100 in methicillin-resistant Staphylococcus aureus","volume":"41","author":"Komatsuzawa","year":"1997","journal-title":"Antimicrob Agents Ch"},{"key":"10.1016\/j.isci.2026.116145_bib63","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.ijmm.2009.10.001","article-title":"The wall teichoic acid and lipoteichoic acid polymers of Staphylococcus aureus","volume":"300","author":"Xia","year":"2010","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib64","doi-asserted-by":"crossref","first-page":"e66376","DOI":"10.7554\/eLife.66376","article-title":"Sensitizing Staphylococcus aureus to antibacterial agents by decoding and blocking the lipid flippase MprF","volume":"11","author":"Slavetinsky","year":"2022","journal-title":"eLife"},{"key":"10.1016\/j.isci.2026.116145_bib65","doi-asserted-by":"crossref","first-page":"e21323","DOI":"10.1371\/journal.pone.0021323","article-title":"Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways","volume":"6","author":"Falord","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2026.116145_bib66","doi-asserted-by":"crossref","DOI":"10.1016\/j.mib.2024.102562","article-title":"Important challenges to finding new leads for new antibiotics","volume":"83","author":"Farha","year":"2025","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib67","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/s41579-018-0098-9","article-title":"Developmental roadmap for antimicrobial susceptibility testing systems","volume":"17","author":"van Belkum","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib68","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1038\/s41467-022-29717-3","article-title":"Human serum triggers antibiotic tolerance in Staphylococcus aureus","volume":"13","author":"Ledger","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.116145_bib69","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1021\/acs.jproteome.2c00073","article-title":"Human Serum Alters the Metabolism and Antibiotic Susceptibility of Staphylococcus aureus","volume":"21","author":"Somerville","year":"2022","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.isci.2026.116145_bib70","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.107927","article-title":"Genetic and Chemical Screening in Human Blood Serum Reveals Unique Antibacterial Targets and Compounds against Klebsiella pneumoniae","volume":"32","author":"Weber","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.isci.2026.116145_bib71","doi-asserted-by":"crossref","first-page":"e0035223","DOI":"10.1128\/spectrum.00352-23","article-title":"Repurposing High-Throughput Screening Identifies Unconventional Drugs with Antibacterial and Antibiofilm Activities against Pseudomonas aeruginosa under Experimental Conditions Relevant to Cystic Fibrosis","volume":"11","author":"Di Bonaventura","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.isci.2026.116145_bib72","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/s41564-019-0595-2","article-title":"Mimicking the human environment in mice reveals that inhibiting biotin biosynthesis is effective against antibiotic-resistant pathogens","volume":"5","author":"Carfrae","year":"2020","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib73","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.ebiom.2017.05.026","article-title":"Correcting a Fundamental Flaw in the Paradigm for Antimicrobial Susceptibility Testing","volume":"20","author":"Ersoy","year":"2017","journal-title":"EBioMedicine"},{"key":"10.1016\/j.isci.2026.116145_bib74","article-title":"Re-evaluation of FDA-approved antibiotics with increased diagnostic accuracy for assessment of antimicrobial resistance","volume":"4","author":"Heithoff","year":"2023","journal-title":"Cell Rep. Med."},{"key":"10.1016\/j.isci.2026.116145_bib75","doi-asserted-by":"crossref","first-page":"4266","DOI":"10.1038\/s41598-025-88048-7","article-title":"Clinical and microbiological analysis of risk factors for breakthrough bloodstream infection during Tigecycline Therapy","volume":"15","author":"Jin","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2026.116145_bib76","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1093\/jac\/dkp455","article-title":"Breakthrough bacteraemia with a susceptible Enterococcus faecalis during tigecycline monotherapy","volume":"65","author":"Parsonage","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.isci.2026.116145_bib77","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.4161\/hv.21872","article-title":"Development of a multicomponent Staphylococcus aureus vaccine designed to counter multiple bacterial virulence factors","volume":"8","author":"Anderson","year":"2012","journal-title":"Hum. Vaccin. Immunother."},{"key":"10.1016\/j.isci.2026.116145_bib78","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1093\/infdis\/jis272","article-title":"Staphylococcus aureus Manganese Transport Protein C Is a Highly Conserved Cell Surface Protein That Elicits Protective Immunity Against S. aureus and Staphylococcus epidermidis","volume":"205","author":"Anderson","year":"2012","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.isci.2026.116145_bib79","doi-asserted-by":"crossref","DOI":"10.1038\/srep33174","article-title":"Exploring the transcriptome of Staphylococcus aureus in its natural niche","volume":"6","author":"Chaves-Moreno","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2026.116145_bib80","doi-asserted-by":"crossref","first-page":"e00715-20","DOI":"10.1128\/IAI.00715-20","article-title":"Analysis of Staphylococcus aureus Transcriptome in Pediatric Soft Tissue Abscesses and Comparison to Murine Infections","volume":"89","author":"Moffitt","year":"2021","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2026.116145_bib81","doi-asserted-by":"crossref","first-page":"e00336-18","DOI":"10.1128\/mSphere.00336-18","article-title":"MntC-Dependent Manganese Transport Is Essential for Staphylococcus aureus Oxidative Stress Resistance and Virulence","volume":"3","author":"Handke","year":"2018","journal-title":"mSphere"},{"key":"10.1016\/j.isci.2026.116145_bib82","doi-asserted-by":"crossref","first-page":"e959","DOI":"10.1542\/peds.2008-2428","article-title":"Empiric antimicrobial therapy for pediatric skin and soft-tissue infections in the era of methicillin-resistant Staphylococcus aureus","volume":"123","author":"Elliott","year":"2009","journal-title":"Pediatrics"},{"key":"10.1016\/j.isci.2026.116145_bib83","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1093\/cid\/cir340","article-title":"Use of Antistaphylococcal \u03b2-Lactams to Increase Daptomycin Activity in Eradicating Persistent Bacteremia Due to Methicillin-Resistant Staphylococcus aureus: Role of Enhanced Daptomycin Binding","volume":"53","author":"Dhand","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.isci.2026.116145_bib84","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s40121-014-0023-0","article-title":"Observation of \u201cseesaw effect\u201d with vancomycin, teicoplanin, daptomycin and ceftaroline in 150 unique MRSA strains","volume":"3","author":"Barber","year":"2014","journal-title":"Infect. Dis. Ther."},{"key":"10.1016\/j.isci.2026.116145_bib85","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1002\/phar.2034","article-title":"Early Administration of Adjuvant \u03b2-Lactam Therapy in Combination with Vancomycin among Patients with Methicillin-Resistant Staphylococcus aureus Bloodstream Infection: A Retrospective, Multicenter Analysis","volume":"37","author":"Casapao","year":"2017","journal-title":"Pharmacotherapy"},{"key":"10.1016\/j.isci.2026.116145_bib86","doi-asserted-by":"crossref","first-page":"e01554-17","DOI":"10.1128\/AAC.01554-17","article-title":"Outcomes of Vancomycin plus a \u03b2-Lactam versus Vancomycin Only for Treatment of Methicillin-Resistant Staphylococcus aureus Bacteremia","volume":"62","author":"Truong","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.isci.2026.116145_bib87","first-page":"a025247","article-title":"\u03b2-Lactams and \u03b2-Lactamase Inhibitors: An Overview","volume":"6","author":"Bush","year":"2016","journal-title":"Csh Perspect Med"},{"key":"10.1016\/j.isci.2026.116145_bib88","doi-asserted-by":"crossref","first-page":"e0234124","DOI":"10.1128\/spectrum.02341-24","article-title":"The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions","volume":"12","author":"Rieger","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.isci.2026.116145_bib89","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1097\/01.ASW.0000416006.55218.d0","article-title":"Acute and impaired wound healing: pathophysiology and current methods for drug delivery, part 1: normal and chronic wounds: biology, causes, and approaches to care","volume":"25","author":"Demidova-Rice","year":"2012","journal-title":"Adv. Skin Wound Care"},{"key":"10.1016\/j.isci.2026.116145_bib90","doi-asserted-by":"crossref","first-page":"e14861","DOI":"10.1111\/iwj.14861","article-title":"The importance of the simulated wound fluid composition and properties in the determination of the fluid handling performance of wound dressings","volume":"21","author":"Svensby","year":"2024","journal-title":"Int. Wound J."},{"key":"10.1016\/j.isci.2026.116145_bib91","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioflm.2021.100047","article-title":"Milieu matters: An in vitro wound milieu to recapitulate key features of, and probe new insights into, mixed-species bacterial biofilms","volume":"3","author":"Kadam","year":"2021","journal-title":"Biofilm"},{"key":"10.1016\/j.isci.2026.116145_bib92","doi-asserted-by":"crossref","first-page":"e112730","DOI":"10.1371\/journal.pone.0112730","article-title":"Staphylococcus aureus manganese transport protein C (MntC) is an extracellular matrix- and plasminogen-binding protein","volume":"9","author":"Salazar","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2026.116145_bib93","series-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically","year":"2012"},{"key":"10.1016\/j.isci.2026.116145_bib94","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/7651_2014_183","article-title":"Method for Preparation and Electroporation of S. aureus and S. epidermidis","volume":"1373","author":"Grosser","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.isci.2026.116145_bib95","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2026.116145_bib96","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.948343","article-title":"Antibacterial activity of novel linear polyamines against Staphylococcus aureus","volume":"13","author":"Douglas","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.isci.2026.116145_bib97","doi-asserted-by":"crossref","first-page":"e3078","DOI":"10.21769\/BioProtoc.3078","article-title":"Extraction and Analysis of Bacterial Teichoic Acids","volume":"8","author":"Kho","year":"2018","journal-title":"Bio. Protoc."},{"key":"10.1016\/j.isci.2026.116145_bib98","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.micron.2013.02.003","article-title":"Imaging living cells surface and quantifying its properties at high resolution using AFM in QI\u2122 mode","volume":"48","author":"Chopinet","year":"2013","journal-title":"Micron"},{"key":"10.1016\/j.isci.2026.116145_bib99","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.chemosphere.2014.12.035","article-title":"Oxidative stress and metabolic perturbations in Escherichia coli exposed to sublethal levels of 2,4-dichlorophenoxyacetic acid","volume":"135","author":"Bhat","year":"2015","journal-title":"Chemosphere"},{"key":"10.1016\/j.isci.2026.116145_bib100","series-title":"Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/978-3-030-83749-5_9","article-title":"Cellulomics of Live Yeast by Advanced and Correlative Microscopy","author":"Shahina","year":"2022"},{"key":"10.1016\/j.isci.2026.116145_bib101","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3762\/bjnano.4.23","article-title":"Determining cantilever stiffness from thermal noise","volume":"4","author":"L\u00fcbbe","year":"2013","journal-title":"Beilstein J Nanotech"}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226015208?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226015208?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:56:29Z","timestamp":1791838589000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004226015208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":101,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S2589004226015208"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2026.116145","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Uncovering the mechanisms of clinically relevant altered antibiotic responses of Staphylococcus aureus under wound infection-mimetic conditions","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2026.116145","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"116145"}}