{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T11:47:30Z","timestamp":1794570450704,"version":"4.9.1"},"reference-count":29,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015542","name":"GlaxoSmithKline Japan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018036","name":"Nippon Shinyaku Co Ltd","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018036","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"The Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008880","name":"Kanae Foundation for the Promotion of Medical Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008880","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["24K12448"],"award-info":[{"award-number":["24K12448"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.isci.2026.116725","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T20:55:31Z","timestamp":1783630531000},"page":"116725","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Single-cell transcriptomics reveals bladder microenvironment dynamics in Hunner type interstitial cystitis"],"prefix":"10.1016","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2599-8183","authenticated-orcid":false,"given":"Fumihiko","family":"Urabe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Yoshihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Hirano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoaki","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hironori","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyaka","family":"Umemori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kagenori","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Igarashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Furuta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2026.116725_bib1","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1111\/iju.14234","article-title":"Clinical guidelines for interstitial cystitis\/bladder pain syndrome","volume":"27","author":"Homma","year":"2020","journal-title":"Int. J. Urol."},{"issue":"Suppl 1","key":"10.1016\/j.isci.2026.116725_bib2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/iju.13969","article-title":"Phenotyping of interstitial cystitis\/bladder pain syndrome","volume":"26","author":"Akiyama","year":"2019","journal-title":"Int. J. Urol."},{"key":"10.1016\/j.isci.2026.116725_bib3","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1016\/S0022-5347(17)40201-1","article-title":"Clinical features and spectrum of light microscopic changes in interstitial cystitis","volume":"143","author":"Johansson","year":"1990","journal-title":"J. Urol."},{"key":"10.1016\/j.isci.2026.116725_bib4","first-page":"25","article-title":"Pathology and terminology of interstitial cystitis\/bladder pain syndrome: A review","volume":"34","author":"Akiyama","year":"2019","journal-title":"Histol. Histopathol."},{"key":"10.1016\/j.isci.2026.116725_bib5","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1002\/nau.23365","article-title":"Digital quantitative analysis of mast cell infiltration in interstitial cystitis","volume":"37","author":"Akiyama","year":"2018","journal-title":"Neurourol. Urodyn."},{"key":"10.1016\/j.isci.2026.116725_bib6","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0143316","article-title":"Hunner-Type (Classic) Interstitial Cystitis: A Distinct Inflammatory Disorder Characterized by Pancystitis, with Frequent Expansion of Clonal B-Cells and Epithelial Denudation","volume":"10","author":"Maeda","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2026.116725_bib7","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1097\/JU.0000000000000234","article-title":"Molecular Taxonomy of Interstitial Cystitis\/Bladder Pain Syndrome Based on Whole Transcriptome Profiling by Next-Generation RNA Sequencing of Bladder Mucosal Biopsies","volume":"202","author":"Akiyama","year":"2019","journal-title":"J. Urol."},{"key":"10.1016\/j.isci.2026.116725_bib8","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/s41392-022-00962-8","article-title":"Integrating single-cell RNA sequencing with spatial transcriptomics reveals immune landscape for interstitial cystitis","volume":"7","author":"Peng","year":"2022","journal-title":"Signal Transduct. Target. Ther."},{"key":"10.1016\/j.isci.2026.116725_bib9","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202106063","article-title":"Multimodal Single-Cell Analyses Outline the Immune Microenvironment and Therapeutic Effectors of Interstitial Cystitis\/Bladder Pain Syndrome","volume":"9","author":"Su","year":"2022","journal-title":"Adv. Sci."},{"key":"10.1016\/j.isci.2026.116725_bib10","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1038\/s41592-019-0619-0","article-title":"Fast, sensitive and accurate integration of single-cell data with Harmony","volume":"16","author":"Korsunsky","year":"2019","journal-title":"Nat. Methods"},{"key":"10.1016\/j.isci.2026.116725_bib11","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s13059-019-1850-9","article-title":"A benchmark of batch-effect correction methods for single-cell RNA sequencing data","volume":"21","author":"Tran","year":"2020","journal-title":"Genome Biol."},{"key":"10.1016\/j.isci.2026.116725_bib12","doi-asserted-by":"crossref","DOI":"10.1038\/srep28652","article-title":"Increased CXCR3 Expression of Infiltrating Plasma Cells in Hunner Type Interstitial Cystitis","volume":"6","author":"Akiyama","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2026.116725_bib13","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1007\/s00192-017-3547-5","article-title":"Comparison of inflammatory urine markers in patients with interstitial cystitis and overactive bladder","volume":"29","author":"Furuta","year":"2018","journal-title":"Int. Urogynecol. J."},{"key":"10.1016\/j.isci.2026.116725_bib14","article-title":"Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis","volume":"14","author":"Zhou","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2026.116725_bib15","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1165\/rcmb.2021-0555OC","article-title":"Anomalous Epithelial Variations and Ectopic Inflammatory Response in Chronic Obstructive Pulmonary Disease","volume":"67","author":"Watanabe","year":"2022","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1016\/j.isci.2026.116725_bib16","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.medj.2022.05.002","article-title":"Cross-tissue, single-cell stromal atlas identifies shared pathological fibroblast phenotypes in four chronic inflammatory diseases","volume":"3","author":"Korsunsky","year":"2022","journal-title":"Med"},{"key":"10.1016\/j.isci.2026.116725_bib17","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-025-22053-8","article-title":"Molecular characterization of chronic inflammatory diseases of the urinary bladder based on next-generation RNA sequencing and digital image analysis","volume":"15","author":"Akiyama","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2026.116725_bib18","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1002\/path.6353","article-title":"APRIL\/BAFF upregulation is associated with clonal B-cell expansion in Hunner-type interstitial cystitis","volume":"264","author":"Horie","year":"2024","journal-title":"J. Pathol."},{"key":"10.1016\/j.isci.2026.116725_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/srep10536","article-title":"The Wnt5a-Ror2 axis promotes the signaling circuit between interleukin-12 and interferon-\u03b3 in colitis","volume":"5","author":"Sato","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2026.116725_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2025.102783","article-title":"HLA-DQB1-mediated B cell-epithelial crosstalk drives EBV-associated inflammation in Hunner-type interstitial cystitis","volume":"36","author":"Thangameeran","year":"2025","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"10.1016\/j.isci.2026.116725_bib21","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cels.2019.03.003","article-title":"DoubletFinder: Doublet Detection in Single-Cell RNA Sequencing Data Using Artificial Nearest Neighbors","volume":"8","author":"McGinnis","year":"2019","journal-title":"Cell Syst."},{"key":"10.1016\/j.isci.2026.116725_bib22","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/s41596-024-01045-4","article-title":"CellChat for systematic analysis of cell-cell communication from single-cell transcriptomics","volume":"20","author":"Jin","year":"2025","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.isci.2026.116725_bib23","doi-asserted-by":"crossref","first-page":"5077","DOI":"10.1038\/s41467-020-18916-5","article-title":"Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma","volume":"11","author":"Chen","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.116725_bib24","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1186\/s12964-023-01312-z","article-title":"Single-cell RNA sequencing reveals pro-invasive cancer-associated fibroblasts in hypopharyngeal squamous cell carcinoma","volume":"21","author":"Cai","year":"2023","journal-title":"Cell Commun. Signal."},{"key":"10.1016\/j.isci.2026.116725_bib25","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1016\/j.ccell.2024.09.011","article-title":"A pan-cancer single-cell RNA-seq atlas of intratumoral B cells","volume":"42","author":"Fitzsimons","year":"2024","journal-title":"Cancer Cell"},{"key":"10.1016\/j.isci.2026.116725_bib26","doi-asserted-by":"crossref","first-page":"2965","DOI":"10.1038\/s41467-021-23324-4","article-title":"Interpretation of T cell states from single-cell transcriptomics data using reference atlases","volume":"12","author":"Andreatta","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.116725_bib27","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1038\/s42003-025-08094-9","article-title":"Single-cell RNA sequencing reveals a fibroblast gene signature that promotes T-cell infiltration in muscle-invasive bladder cancer","volume":"8","author":"Liu","year":"2025","journal-title":"Commun. Biol."},{"key":"10.1016\/j.isci.2026.116725_bib28","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: an R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"OMICS"},{"key":"10.1016\/j.isci.2026.116725_bib29","doi-asserted-by":"crossref","first-page":"3236","DOI":"10.1158\/0008-5472.CAN-22-0090","article-title":"Single-Cell Transcriptome Profiling Reveals Intratumoral Heterogeneity and Molecular Features of Ductal Carcinoma In Situ","volume":"82","author":"Tokura","year":"2022","journal-title":"Cancer Res."}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226021036?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226021036?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T18:12:44Z","timestamp":1794247964000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004226021036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":29,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["S2589004226021036"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2026.116725","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-cell transcriptomics reveals bladder microenvironment dynamics in Hunner type interstitial cystitis","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2026.116725","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"116725"}}