{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T18:24:04Z","timestamp":1794248644059,"version":"4.9.0"},"reference-count":66,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["AZR02170"],"award-info":[{"award-number":["AZR02170"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.isci.2026.117140","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T12:14:38Z","timestamp":1786450478000},"page":"117140","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineered HMGB1 construct with tandem Box B domains promotes tissue regeneration without potential for inflammation"],"prefix":"10.1016","volume":"29","author":[{"given":"\u00c1lvaro","family":"Vi\u00f1als Guitart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Isabel","family":"Esp\u00edrito Santo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Ling","family":"Ruan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Hsuan","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn","family":"William","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Redfield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg","family":"Fedorov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola A.","family":"Burgess-Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wyatt W.","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9579-9411","authenticated-orcid":false,"given":"Jagdeep","family":"Nanchahal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2026.117140_bib1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0092-8674(00)81692-X","article-title":"Stem cells: units of development, units of regeneration, and units in evolution","volume":"100","author":"Weissman","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.isci.2026.117140_bib2","doi-asserted-by":"crossref","first-page":"25476","DOI":"10.3390\/ijms161025476","article-title":"Stem cells in skin regeneration, wound healing, and their clinical applications","volume":"16","author":"Ojeh","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.isci.2026.117140_bib3","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S2352-3026(15)00028-9","article-title":"One million haemopoietic stem-cell transplants: a retrospective observational study","volume":"2","author":"Gratwohl","year":"2015","journal-title":"Lancet Haematol."},{"key":"10.1016\/j.isci.2026.117140_bib4","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1038\/s41586-019-1802-2","article-title":"An acute immune response underlies the benefit of cardiac stem cell therapy","volume":"577","author":"Vagnozzi","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.isci.2026.117140_bib5","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/0142-9612(95)93260-K","article-title":"In vivo and in vitro elimination of aliphatic polyanhydrides","volume":"16","author":"Domb","year":"1995","journal-title":"Biomaterials"},{"key":"10.1016\/j.isci.2026.117140_bib6","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1038\/nbt.2970","article-title":"Rethinking clinical delivery of adult stem cell therapies","volume":"32","author":"Trainor","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.isci.2026.117140_bib7","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/nm.3653","article-title":"Preparing the ground for tissue regeneration: from mechanism to therapy","volume":"20","author":"Forbes","year":"2014","journal-title":"Nat. Med."},{"key":"10.1016\/j.isci.2026.117140_bib8","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1038\/nbt.2978","article-title":"Modulating the stem cell niche for tissue regeneration","volume":"32","author":"Lane","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.isci.2026.117140_bib9","doi-asserted-by":"crossref","first-page":"aaa2340","DOI":"10.1126\/science.aaa2340","article-title":"Inhibition of the prostaglandin-degrading enzyme 15-PGDH potentiates tissue regeneration","volume":"348","author":"Zhang","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.isci.2026.117140_bib10","first-page":"4463","article-title":"Fully reduced HMGB1 accelerates the regeneration of multiple tissues by transitioning stem cells to G Alert","volume":"115","author":"Lee","year":"2018","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.isci.2026.117140_bib11","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1084\/jem.20120189","article-title":"Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release","volume":"209","author":"Venereau","year":"2012","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.isci.2026.117140_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0079572","article-title":"Role of the acidic tail of high mobility group protein B1 (HMGB1) in protein stability and DNA bending","volume":"8","author":"Belgrano","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2026.117140_bib13","doi-asserted-by":"crossref","first-page":"10161","DOI":"10.1093\/nar\/gks815","article-title":"Nucleosome dynamics: HMGB1 relaxes canonical nucleosome structure to facilitate estrogen receptor binding","volume":"40","author":"Joshi","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2026.117140_bib14","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.coi.2005.06.002","article-title":"Alarmins: Chemotactic activators of immune responses","volume":"17","author":"Oppenheim","year":"2005","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.isci.2026.117140_bib15","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1007\/978-1-59745-198-7_81","article-title":"The Use of Infrared Fluorescent Dyes in Immunofluorescence Microscopy","author":"Kasbek","year":"2009","journal-title":"Protein Protocols Handbook. Springer Protocols Handbooks."},{"key":"10.1016\/j.isci.2026.117140_bib16","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/nature13255","article-title":"mTORC1 controls the adaptive transition of quiescent stem cells from G0 to G(Alert","volume":"510","author":"Rodgers","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.isci.2026.117140_bib17","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.3389\/fimmu.2018.02118","article-title":"Redox-Mediated Mechanisms Fuel Monocyte Responses to CXCL12\/HMGB1 in Active Rheumatoid Arthritis","volume":"9","author":"Cecchinato","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2026.117140_bib18","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.3389\/fimmu.2020.01122","article-title":"Oxidation of HMGB1 Is a Dynamically Regulated Process in Physiological and Pathological Conditions","volume":"11","author":"Ferrara","year":"2020","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2026.117140_bib19","doi-asserted-by":"crossref","first-page":"11621","DOI":"10.1074\/jbc.M113.449942","article-title":"Real-time kinetics of high-mobility group box 1 (HMGB1) oxidation in extracellular fluids studied by in situ protein NMR spectroscopy","volume":"288","author":"L","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117140_bib20","doi-asserted-by":"crossref","first-page":"5551","DOI":"10.1093\/emboj\/cdg516","article-title":"Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretion","volume":"22","author":"Bonaldi","year":"2003","journal-title":"EMBO J."},{"key":"10.1016\/j.isci.2026.117140_bib21","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1242\/jcs.176412","article-title":"N-linked glycosylation plays a crucial role in the secretion of HMGB1","volume":"129","author":"Kim","year":"2016","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.isci.2026.117140_bib22","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1159\/000369972","article-title":"HMGB1 Binds to Lipoteichoic Acid and Enhances TNF-\u03b1 and IL-6 Production through HMGB1-Mediated Transfer of Lipoteichoic Acid to CD14 and TLR2","volume":"7","author":"Kwak","year":"2015","journal-title":"J. Innate Immun."},{"key":"10.1016\/j.isci.2026.117140_bib23","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1097\/01.shk.0000225404.51320.82","article-title":"HMGB1 signals through toll-like receptor (TLR) 4 and TLR2","volume":"26","author":"Yu","year":"2006","journal-title":"Shock"},{"key":"10.1016\/j.isci.2026.117140_bib24","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s10020-018-0021-x","article-title":"Ligation of free HMGB1 to TLR2 in the absence of ligand is negatively regulated by the C-terminal tail domain","volume":"24","author":"Aucott","year":"2018","journal-title":"Mol. Med."},{"key":"10.1016\/j.isci.2026.117140_bib25","doi-asserted-by":"crossref","first-page":"11942","DOI":"10.1073\/pnas.1003893107","article-title":"A critical cysteine is required for HMGB1 binding to Toll-like receptor 4 and activation of macrophage cytokine release","volume":"107","author":"Yang","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2026.117140_bib26","first-page":"4805","article-title":"Receptor for advanced glycation end products-binding COOH-terminal motif of amphoterin inhibits invasive migration and metastasis","volume":"62","author":"Huttunen","year":"2002","journal-title":"Cancer Res."},{"key":"10.1016\/j.isci.2026.117140_bib27","doi-asserted-by":"crossref","first-page":"25752","DOI":"10.1074\/jbc.270.43.25752","article-title":"The Receptor for Advanced Glycation End Products (RAGE) Is a Cellular Binding Site for Amphoterin","volume":"270","author":"Hori","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117140_bib28","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nri1594","article-title":"High-mobility group box 1 protein (HMGB1): nuclear weapon in the immune arsenal","volume":"5","author":"Lotze","year":"2005","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.isci.2026.117140_bib29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0023132","article-title":"TIRAP, an adaptor protein for TLR2\/4, transduces a signal from RAGE phosphorylated upon ligand binding","volume":"6","author":"Sakaguchi","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2026.117140_bib30","doi-asserted-by":"crossref","first-page":"705","DOI":"10.3389\/fimmu.2018.00705","article-title":"High-mobility group box 1-induced complement activation causes sterile inflammation","volume":"9","author":"Kim","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2026.117140_bib31","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.molimm.2012.10.037","article-title":"HMGB1 and leukocyte migration during trauma and sterile inflammation","volume":"55","author":"Venereau","year":"2013","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.isci.2026.117140_bib32","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1182\/blood-2016-04-710632","article-title":"Disulfide HMGB1 derived from platelets coordinates venous thrombosis in mice","volume":"128","author":"Stark","year":"2016","journal-title":"Blood"},{"key":"10.1016\/j.isci.2026.117140_bib33","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1160\/TH14-12-1073","article-title":"HMGB1 binds to activated platelets via the receptor for advanced glycation end products and is present in platelet rich human coronary artery thrombi","volume":"114","author":"Ahrens","year":"2015","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.isci.2026.117140_bib34","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1084\/jem.20160217","article-title":"High mobility group box 1 orchestrates tissue regeneration via CXCR4","volume":"215","author":"Tirone","year":"2018","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.isci.2026.117140_bib35","doi-asserted-by":"crossref","first-page":"2693","DOI":"10.1016\/j.bbadis.2017.07.012","article-title":"Non-oxidizable HMGB1 induces cardiac fibroblasts migration via CXCR4 in a CXCL12-independent manner and worsens tissue remodeling after myocardial infarction","volume":"1863","author":"Di Maggio","year":"2017","journal-title":"Biochim. Biophys. Acta. Mol. Basis Dis."},{"key":"10.1016\/j.isci.2026.117140_bib36","first-page":"e47788","article-title":"Diflunisal targets the HMGB1\/CXCL12 heterocomplex and blocks immune cell recruitment - PubMed","volume":"20","author":"F","year":"2019","journal-title":"EMBO Rep."},{"key":"10.1016\/j.isci.2026.117140_bib37","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.chembiol.2007.03.007","article-title":"Glycyrrhizin binds to high-mobility group box 1 protein and inhibits its cytokine activities","volume":"14","author":"Mollica","year":"2007","journal-title":"Chem. Biol."},{"key":"10.1016\/j.isci.2026.117140_bib38","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1002\/j.1460-2075.1992.tb05144.x","article-title":"The DNA binding site of HMG1 protein is composed of two similar segments (HMG boxes), both of which have counterparts in other eukaryotic regulatory proteins","volume":"11","author":"Bianchi","year":"1992","journal-title":"EMBO J."},{"key":"10.1016\/j.isci.2026.117140_bib39","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1084\/jem.20111739","article-title":"HMGB1 promotes recruitment of inflammatory cells to damaged tissues by forming a complex with CXCL12 and signaling via CXCR4","volume":"209","author":"Schiraldi","year":"2012","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.isci.2026.117140_bib40","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1016\/j.csbj.2019.06.020","article-title":"Oxidation State Dependent Conformational Changes of HMGB1 Regulate the Formation of the CXCL12\/HMGB1 Heterocomplex","volume":"17","author":"Fassi","year":"2019","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"10.1016\/j.isci.2026.117140_bib41","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1038\/s41467-024-45505-7","article-title":"The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with CXCL12","volume":"15","author":"Mantonico","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.117140_bib42","doi-asserted-by":"crossref","first-page":"7777","DOI":"10.1074\/jbc.M113.541474","article-title":"An immunogenic peptide in the A-box of HMGB1 protein reverses apoptosis-induced tolerance through RAGE receptor","volume":"289","author":"LeBlanc","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117140_bib43","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.ejps.2017.12.019","article-title":"Production and application of HMGB1 derived recombinant RAGE-antagonist peptide for anti-inflammatory therapy in acute lung injury","volume":"114","author":"Lee","year":"2018","journal-title":"Eur. J. Pharmaceut. Sci."},{"key":"10.1016\/j.isci.2026.117140_bib44","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1002\/eji.201141391","article-title":"Identification of lipopolysaccharide-binding peptide regions within HMGB1 and their effects on subclinical endotoxemia in a mouse model","volume":"41","author":"Youn","year":"2011","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.isci.2026.117140_bib45","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.bbrc.2013.10.085","article-title":"Redox-sensitive structural change in the A-domain of HMGB1 and its implication for the binding to cisplatin modified DNA","volume":"441","author":"Wang","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.isci.2026.117140_bib46","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s10020-018-0023-8","article-title":"Exploring the biological functional mechanism of the HMGB1\/TLR4\/MD-2 complex by surface plasmon resonance","volume":"24","author":"He","year":"2018","journal-title":"Mol. Med."},{"key":"10.1016\/j.isci.2026.117140_bib47","doi-asserted-by":"crossref","first-page":"e614","DOI":"10.1097\/CCM.0000000000003800","article-title":"Biphasic Release of the Alarmin High Mobility Group Box 1 Protein Early After Trauma Predicts Poor Clinical Outcome","volume":"47","author":"Ottestad","year":"2019","journal-title":"Crit. Care Med."},{"key":"10.1016\/j.isci.2026.117140_bib49","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1089\/thy.2016.0432","article-title":"Glycyrrhizin, a Direct HMGB1 Antagonist, Ameliorates Inflammatory Infiltration in a Model of Autoimmune Thyroiditis via Inhibition of TLR2-HMGB1 Signaling","volume":"27","author":"Li","year":"2017","journal-title":"Thyroid"},{"key":"10.1016\/j.isci.2026.117140_bib50","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1110\/ps.041219505","article-title":"The monomer \u2013 dimer equilibrium of stromal cell-derived factor-1 (CXCL12) is altered by pH, phosphate, sulfate, and heparin","volume":"14","author":"Veldkamp","year":"2005","journal-title":"Protein Sci."},{"key":"10.1016\/j.isci.2026.117140_bib51","doi-asserted-by":"crossref","first-page":"6996","DOI":"10.1093\/emboj\/16.23.6996","article-title":"Solution structure and basis for functional activity of stromal cell -derived factor -1; dissociation of CXCR4 activation from binding and inhibition of HIV -1","volume":"16","author":"Crump","year":"1997","journal-title":"EMBO J."},{"key":"10.1016\/j.isci.2026.117140_bib52","doi-asserted-by":"crossref","first-page":"4582","DOI":"10.1128\/JVI.73.6.4582-4589.1999","article-title":"Enhanced Inhibition of Human Immunodeficiency Virus Type 1 by Met-Stromal-Derived Factor 1\u03b2 Correlates with Down-Modulation of CXCR4","volume":"73","author":"Yang","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/j.isci.2026.117140_bib53","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12934-016-0617-1","article-title":"High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery","volume":"16","author":"Turchetto","year":"2017","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.isci.2026.117140_bib54","doi-asserted-by":"crossref","first-page":"6135","DOI":"10.1021\/pr1002438","article-title":"Efficient Subfractionation of Gram-Negative Bacteria for Proteomics Studies","volume":"9","author":"Thein","year":"2010","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.isci.2026.117140_bib55","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1110\/ps.041219505","article-title":"The monomer\u2013dimer equilibrium of stromal cell-derived factor-1 (CXCL 12) is altered by pH, phosphate, sulfate, and heparin","volume":"14","author":"Veldkamp","year":"2005","journal-title":"Protein Sci."},{"key":"10.1016\/j.isci.2026.117140_bib56","doi-asserted-by":"crossref","first-page":"10018","DOI":"10.1074\/jbc.M608796200","article-title":"Structural and Functional Basis of CXCL12 (Stromal Cell-derived Factor-1\u03b1) Binding to Heparin","volume":"282","author":"Murphy","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117140_bib57","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0022-1759(90)90029-U","article-title":"Removal of endotoxin from protein solutions by phase separation using triton X-114","volume":"132","author":"Aida","year":"1990","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.isci.2026.117140_bib58","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0173778","article-title":"Optimized Triton X-114 assisted lipopolysaccharide (LPS) removal method reveals the immunomodulatory effect of food proteins","volume":"12","author":"Teodorowicz","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2026.117140_bib59","series-title":"Heterologous Gene Expression in E.coli: Methods and Protocols","first-page":"373","article-title":"Mass Spectrometric Analysis of Proteins","author":"Chalk","year":"2017"},{"key":"10.1016\/j.isci.2026.117140_bib60","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jsb.2010.06.008","article-title":"High-throughput production of human proteins for crystallization: The SGC experience","volume":"172","author":"Savitsky","year":"2010","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.isci.2026.117140_bib61","doi-asserted-by":"crossref","first-page":"159","DOI":"10.3390\/chromatography1040159","article-title":"High-Throughput Mass Spectrometry Applied to Structural Genomics","volume":"1","author":"Chalk","year":"2014","journal-title":"Chromatography"},{"key":"10.1016\/j.isci.2026.117140_bib62","doi-asserted-by":"crossref","first-page":"5370","DOI":"10.1021\/ac050511+","article-title":"Estimates of Protein Surface Areas in Solution by Electrospray Ionization Mass Spectrometry","volume":"77","author":"Kaltashov","year":"2005","journal-title":"Anal. Chem."},{"key":"10.1016\/j.isci.2026.117140_bib63","doi-asserted-by":"crossref","first-page":"6459","DOI":"10.1021\/ac201740z","article-title":"Charge-Surface Correlation in Electrospray Ionization of Folded and Unfolded Proteins","volume":"83","author":"Testa","year":"2011","journal-title":"Anal. Chem."},{"key":"10.1016\/j.isci.2026.117140_bib64","first-page":"27","article-title":"Contribution: Protein Array Analyzer for ImageJ","volume":"10","author":"Carpentier","year":"2010","journal-title":"ImageJ. news"},{"key":"10.1016\/j.isci.2026.117140_bib65","series-title":"CelluSpotsTM: A Reproducible Means of Making Peptide Arrays for the Determination of SH2 Domain Binding Specificity BT - Peptide Microarrays: Methods and Protocols","author":"Wu","year":"2009"},{"key":"10.1016\/j.isci.2026.117140_bib66","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s10858-016-0060-y","article-title":"CcpNmr AnalysisAssign: a flexible platform for integrated NMR analysis - PubMed","volume":"66","author":"SP","year":"2016","journal-title":"J. Biomol. NMR"},{"key":"10.1016\/j.isci.2026.117140_bib67","doi-asserted-by":"crossref","first-page":"353","DOI":"10.4097\/kja.d.18.00242","article-title":"Multiple Comparison Test and Its Imitations What is the proper way to apply the multiple comparison test? KJA","volume":"71","author":"Lee","year":"2018","journal-title":"Korean J. Anesthesiol."}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226025186?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226025186?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T18:07:37Z","timestamp":1794247657000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004226025186"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":66,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["S2589004226025186"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2026.117140","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineered HMGB1 construct with tandem Box B domains promotes tissue regeneration without potential for inflammation","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2026.117140","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117140"}}