{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:50:09Z","timestamp":1791597009198,"version":"4.3.4"},"reference-count":24,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008990","name":"Science and Technology Department of Zhejiang Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008990","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.isci.2026.117190","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T04:34:17Z","timestamp":1787373257000},"page":"117190","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Claudin-12 sustains urothelial barrier defense against uropathogenic E. coli via calcium-dependent immunomodulation"],"prefix":"10.1016","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8049-1281","authenticated-orcid":false,"given":"Yanpeng","family":"Tian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongping","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhui","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longcheng","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2026.117190_bib1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23042194","article-title":"Specific impacts of ketamine on bladder dysfunction and associated histological alterations in rats-a time course validation through transmission electron microscopy","volume":"23","author":"Chung","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.isci.2026.117190_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/diagnostics12082009","article-title":"Changes in cystoscopic findings after intravesical hyaluronic acid instillation therapy in patients with interstitial cystitis","volume":"12","author":"Lin","year":"2022","journal-title":"Diagnostics"},{"key":"10.1016\/j.isci.2026.117190_bib3","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines10020245","article-title":"Bladder ultrastructure and urinary cytokine abnormality in patients with recurrent urinary tract infection and the changes after intravesical platelet-rich plasma injections","volume":"10","author":"Jhang","year":"2022","journal-title":"Biomedicines"},{"key":"10.1016\/j.isci.2026.117190_bib4","doi-asserted-by":"crossref","first-page":"19884","DOI":"10.1073\/pnas.0606329104","article-title":"Filamentation by escherichia coli subverts innate defenses during urinary tract infection","volume":"103","author":"Justice","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2026.117190_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2023.1128132","article-title":"An immunoresponsive three-dimensional urine-tolerant human urothelial model to study urinary tract infection","volume":"13","author":"Jafari","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.isci.2026.117190_bib6","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.85902","article-title":"Claudin-low bladder tumors are immune infiltrated and actively immune suppressed","volume":"1","author":"Kardos","year":"2016","journal-title":"JCI Insight"},{"key":"10.1016\/j.isci.2026.117190_bib7","doi-asserted-by":"crossref","first-page":"7844","DOI":"10.1128\/IAI.73.12.7844-7852.2005","article-title":"Helicobacter pylori activates myosin light-chain kinase to disrupt claudin-4 and claudin-5 and increase epithelial permeability","volume":"73","author":"Fedwick","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.isci.2026.117190_bib8","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1111\/j.1462-5822.2010.01429.x","article-title":"Interleukin-1 receptor phosphorylation activates rho kinase to disrupt human gastric tight junctional claudin-4 during helicobacter pylori infection","volume":"12","author":"Lapointe","year":"2010","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.isci.2026.117190_bib9","doi-asserted-by":"crossref","first-page":"F305","DOI":"10.1152\/ajprenal.00341.2003","article-title":"Distribution of the tight junction proteins zo-1, occludin, and claudin-4, -8, and -12 in bladder epithelium","volume":"287","author":"Acharya","year":"2004","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"10.1016\/j.isci.2026.117190_bib10","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2111247118","article-title":"Claudin-2 and claudin-12 form independent, complementary pores required to maintain calcium homeostasis","volume":"118","author":"Beggs","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2026.117190_bib11","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.3390\/ijms21062074","article-title":"Claudin-12 knockout mice demonstrate reduced proximal tubule calcium permeability","volume":"21","author":"Plain","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.isci.2026.117190_bib12","article-title":"Role of uropathogenic escherichia coli virulence factors in development of urinary tract infection and kidney damage","volume":"2012","author":"Bien","year":"2012","journal-title":"Internet J. Nephrol."},{"key":"10.1016\/j.isci.2026.117190_bib13","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1038\/s41564-023-01346-6","article-title":"Uropathogenic escherichia coli infection-induced epithelial trained immunity impacts urinary tract disease outcome","volume":"8","author":"Russell","year":"2023","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.isci.2026.117190_bib14","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1007\/s11033-025-10799-3","article-title":"Dynamics of urinary tract infections: a comprehensive study on antimicrobial susceptibility, virulence profiling and molecular epidemiology of uropathogenic escherichia coli from pakistan","volume":"52","author":"Ashraf","year":"2025","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.isci.2026.117190_bib15","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1083\/jcb.200110122","article-title":"Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice","volume":"156","author":"Furuse","year":"2002","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.isci.2026.117190_bib16","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/s41579-020-0324-0","article-title":"Urinary tract infections: microbial pathogenesis, host-pathogen interactions and new treatment strategies","volume":"18","author":"Klein","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.isci.2026.117190_bib17","doi-asserted-by":"crossref","DOI":"10.3390\/cells11071129","article-title":"Vitamin d receptor influences intestinal barriers in health and disease","volume":"11","author":"Sun","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.isci.2026.117190_bib18","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/nyas.13360","article-title":"Intestinal epithelial claudins: expression and regulation in homeostasis and inflammation","volume":"1397","author":"Garcia-Hernandez","year":"2017","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.isci.2026.117190_bib19","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.jcmgh.2019.03.003","article-title":"Deficiency of stomach-type claudin-18 in mice induces gastric tumor formation independent of h pylori infection","volume":"8","author":"Suzuki","year":"2019","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.isci.2026.117190_bib20","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1165\/rcmb.2013-0353OC","article-title":"Knockout mice reveal key roles for claudin 18 in alveolar barrier properties and fluid homeostasis","volume":"51","author":"Li","year":"2014","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1016\/j.isci.2026.117190_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2024.e39338","article-title":"Role of mir-455-3p in the alleviation of lps-induced acute lung injury by allicin","volume":"10","author":"Zheng","year":"2024","journal-title":"Heliyon"},{"key":"10.1016\/j.isci.2026.117190_bib22","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1158\/0008-5472.CAN-21-3896","article-title":"Claudin-12 deficiency inhibits tumor growth by impairing transendothelial migration of myeloid-derived suppressor cells","volume":"82","author":"Cao","year":"2022","journal-title":"Cancer Res."},{"key":"10.1016\/j.isci.2026.117190_bib23","article-title":"Exosomes derived from bladder epithelial cells infected with uropathogenic escherichia coli increase the severity of urinary tract infections (utis) by impairing macrophage function","volume":"20","author":"Wang","year":"2024","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.isci.2026.117190_bib24","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.7150\/jca.36438","article-title":"Alteration of cyp4a11 expression in renal cell carcinoma: diagnostic and prognostic implications","volume":"11","author":"Kim","year":"2020","journal-title":"J. Cancer"}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S258900422602568X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S258900422602568X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:07:25Z","timestamp":1791594445000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S258900422602568X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":24,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S258900422602568X"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2026.117190","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Claudin-12 sustains urothelial barrier defense against uropathogenic E. coli via calcium-dependent immunomodulation","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2026.117190","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117190"}}