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Evol."},{"key":"10.1016\/j.isci.2026.117402_bib77","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1146\/annurev-ento-022024-011250","article-title":"The Evolutionary Biology of Chelicerata","volume":"70","author":"Sharma","year":"2025","journal-title":"Annu. Rev. Entomol."},{"key":"10.1016\/j.isci.2026.117402_bib78","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1534\/genetics.106.058289","article-title":"Drosophila mus301\/spindle-C Encodes a Helicase With an Essential Role in Double-Strand DNA Break Repair and Meiotic Progression","volume":"174","author":"McCaffrey","year":"2006","journal-title":"Genetics"},{"key":"10.1016\/j.isci.2026.117402_bib79","doi-asserted-by":"crossref","first-page":"8716","DOI":"10.1074\/jbc.M110271200","article-title":"A Human DNA Helicase Homologous to the DNA Cross-link Sensitivity Protein Mus308","volume":"277","author":"Marini","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117402_bib80","doi-asserted-by":"crossref","DOI":"10.1093\/genetics\/iyad211","article-title":"FlyBase: updates to the Drosophila genes and genomes database","volume":"227","author":"\u00d6zt\u00fcrk-\u00c7olak","year":"2024","journal-title":"Genetics"},{"key":"10.1016\/j.isci.2026.117402_bib81","doi-asserted-by":"crossref","first-page":"D789","DOI":"10.1093\/nar\/gku1205","article-title":"OMIM.org: Online Mendelian Inheritance in Man (OMIM\u00ae), an online catalog of human genes and genetic disorders","volume":"43","author":"Amberger","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2026.117402_bib82","doi-asserted-by":"crossref","first-page":"E5871","DOI":"10.1073\/pnas.1706865114","article-title":"Disruption of a horizontally transferred phytoene desaturase abolishes carotenoid accumulation and diapause in Tetranychus urticae","volume":"114","author":"Bryon","year":"2017","journal-title":"Proc. Natl. Acad. Sci. 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Genet."},{"key":"10.1016\/j.isci.2026.117402_bib85","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1111\/j.1558-5646.2007.00052.x","article-title":"THE BUILD UP OF MUTATION\u2013SELECTION\u2013 DRIFT BALANCE IN LABORATORY DROSOPHILA POPULATIONS","volume":"61","author":"Garc\u00eda-Dorado","year":"2007","journal-title":"Evolution"},{"key":"10.1016\/j.isci.2026.117402_bib86","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1534\/genetics.113.158220","article-title":"Identifying Signatures of Selection in Genetic Time Series","volume":"196","author":"Feder","year":"2014","journal-title":"Genetics"},{"key":"10.1016\/j.isci.2026.117402_bib87","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1098\/rsbl.2010.0580","article-title":"On measuring selection in experimental evolution","volume":"7","author":"Chevin","year":"2011","journal-title":"Biol. Lett."},{"key":"10.1016\/j.isci.2026.117402_bib88","doi-asserted-by":"crossref","first-page":"7394","DOI":"10.1038\/ncomms8394","article-title":"Polymerase \u0398 is a key driver of genome evolution and of CRISPR\/Cas9-mediated mutagenesis","volume":"6","author":"van Schendel","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.117402_bib89","doi-asserted-by":"crossref","first-page":"gkaf1532","DOI":"10.1093\/nar\/gkaf1532","article-title":"Microhomology-mediated end joining is the predominant form of DNA repair in the mosquito Aedes aegypti with implications for gene editing, gene drive, and transgene removal","volume":"54","author":"Romanowski","year":"2026","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2026.117402_bib90","doi-asserted-by":"crossref","first-page":"4886","DOI":"10.1038\/s41598-025-88564-6","article-title":"Dynamic interplay of cNHEJ and MMEJ pathways of DNA double-strand break repair during embryonic development in zebrafish","volume":"15","author":"Carrara","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2026.117402_bib91","doi-asserted-by":"crossref","first-page":"5295","DOI":"10.1038\/s41467-022-32743-w","article-title":"Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks","volume":"13","author":"van de Kooij","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.117402_bib92","doi-asserted-by":"crossref","DOI":"10.1242\/dmm.035352","article-title":"CRISPR\/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments","volume":"11","author":"Boel","year":"2018","journal-title":"Dis. Model. Mech."},{"key":"10.1016\/j.isci.2026.117402_bib93","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.1038\/ncomms4216","article-title":"A Polymerase Theta-dependent repair pathway suppresses extensive genomic instability at endogenous G4 DNA sites","volume":"5","author":"Koole","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.117402_bib94","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/j.tig.2019.06.001","article-title":"Templated Insertions: A Smoking Gun for Polymerase Theta-Mediated End Joining","volume":"35","author":"Schimmel","year":"2019","journal-title":"Trends Genet."},{"key":"10.1016\/j.isci.2026.117402_bib95","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1002\/em.22564","article-title":"Templated insertions\u2014DNA repair gets acrobatic","volume":"65","author":"Stroik","year":"2024","journal-title":"Environ. Mol. Mutagen."},{"key":"10.1016\/j.isci.2026.117402_bib96","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/nsmb.2961","article-title":"Mechanism of microhomology-mediated end-joining promoted by human DNA polymerase \u03b8","volume":"22","author":"Kent","year":"2015","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.isci.2026.117402_bib97","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1006813","article-title":"Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair","volume":"13","author":"Beagan","year":"2017","journal-title":"PLoS Genet."},{"key":"10.1016\/j.isci.2026.117402_bib98","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1001005","article-title":"Dual Roles for DNA Polymerase Theta in Alternative End-Joining Repair of Double-Strand Breaks in Drosophila","volume":"6","author":"Chan","year":"2010","journal-title":"PLoS Genet."},{"key":"10.1016\/j.isci.2026.117402_bib99","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.molcel.2016.06.020","article-title":"Essential Roles for Polymerase \u03b8-Mediated End Joining in the Repair of Chromosome Breaks","volume":"63","author":"Wyatt","year":"2016","journal-title":"Mol. 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Biol."},{"key":"10.1016\/j.isci.2026.117402_bib103","doi-asserted-by":"crossref","first-page":"E10745","DOI":"10.1073\/pnas.1711979114","article-title":"Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks","volume":"114","author":"Paix","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2026.117402_bib104","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-98965-y","article-title":"Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair","volume":"11","author":"Schubert","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2026.117402_bib105","doi-asserted-by":"crossref","first-page":"2945","DOI":"10.1093\/nar\/gky076","article-title":"DNA mismatch repair and oligonucleotide end-protection promote base-pair substitution distal from a CRISPR\/Cas9-induced DNA break","volume":"46","author":"Harmsen","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2026.117402_bib106","first-page":"52","article-title":"Transient suppression of MLH1 allows effective single-nucleotide substitution by single-stranded DNA oligonucleotides","volume":"715","author":"Dekker","year":"2011","journal-title":"Mutat. Res."},{"key":"10.1016\/j.isci.2026.117402_bib107","doi-asserted-by":"crossref","DOI":"10.1242\/dev.199994","article-title":"Knock-in tagging in zebrafish facilitated by insertion into non-coding regions","volume":"148","author":"Levic","year":"2021","journal-title":"Development"},{"key":"10.1016\/j.isci.2026.117402_bib108","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s13059-017-1220-4","article-title":"Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins","volume":"18","author":"Quadros","year":"2017","journal-title":"Genome Biol."},{"key":"10.1016\/j.isci.2026.117402_bib109","article-title":"Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs","volume":"44","author":"Paix","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2026.117402_bib110","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1186\/s12864-023-09377-3","article-title":"ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells","volume":"24","author":"Mabuchi","year":"2023","journal-title":"BMC Genom."},{"key":"10.1016\/j.isci.2026.117402_bib111","doi-asserted-by":"crossref","DOI":"10.3389\/fgeed.2023.1068637","article-title":"In search of an ideal template for therapeutic genome editing: A review of current developments for structure optimization","volume":"5","author":"Shakirova","year":"2023","journal-title":"Front. Genome Ed."},{"key":"10.1016\/j.isci.2026.117402_bib112","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s13059-017-1164-8","article-title":"Efficient precise knockin with a double cut HDR donor after CRISPR\/Cas9-mediated double-stranded DNA cleavage","volume":"18","author":"Zhang","year":"2017","journal-title":"Genome Biol."},{"key":"10.1016\/j.isci.2026.117402_bib113","doi-asserted-by":"crossref","DOI":"10.3389\/fnins.2020.00838","article-title":"Novel Therapeutic Approaches for the Treatment of Retinal Degenerative Diseases: Focus on CRISPR\/Cas-Based Gene Editing","volume":"14","author":"Gallego","year":"2020","journal-title":"Front. Neurosci."},{"key":"10.1016\/j.isci.2026.117402_bib114","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/s13578-023-01159-4","article-title":"Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair","volume":"13","author":"Li","year":"2023","journal-title":"Cell Biosci."},{"key":"10.1016\/j.isci.2026.117402_bib115","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2024.102344","article-title":"CRISPR-Cas9-mediated homology-directed repair for precise gene editing","volume":"35","author":"Liao","year":"2024","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"10.1016\/j.isci.2026.117402_bib116","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1101\/gr.161638.113","article-title":"Highly efficient CRISPR\/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair","volume":"24","author":"Auer","year":"2014","journal-title":"Genome Res."},{"key":"10.1016\/j.isci.2026.117402_bib117","doi-asserted-by":"crossref","first-page":"5560","DOI":"10.1038\/ncomms6560","article-title":"Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR\/Cas9","volume":"5","author":"Nakade","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.117402_bib118","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1038\/s12276-025-01442-z","article-title":"Gene editing and CRISPR-dependent homology-mediated end joining","volume":"57","author":"Ruis","year":"2025","journal-title":"Exp. Mol. Med."},{"key":"10.1016\/j.isci.2026.117402_bib119","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.ydbio.2022.06.001","article-title":"CRISPR\/Cas9-based simple transgenesis in Xenopus laevis","volume":"489","author":"Shibata","year":"2022","journal-title":"Dev. Biol."},{"key":"10.1016\/j.isci.2026.117402_bib120","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s00439-020-02198-4","article-title":"Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats","volume":"140","author":"Yoshimi","year":"2021","journal-title":"Hum. Genet."},{"key":"10.1016\/j.isci.2026.117402_bib121","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.39468","article-title":"Efficient single-copy HDR by 5\u2019 modified long dsDNA donors","volume":"7","author":"Gutierrez-Triana","year":"2018","journal-title":"eLife"},{"key":"10.1016\/j.isci.2026.117402_bib122","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/s41589-019-0432-1","article-title":"An efficient gene knock-in strategy using 5\u2032-modified double-stranded DNA donors with short homology arms","volume":"16","author":"Yu","year":"2020","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.isci.2026.117402_bib123","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1111\/eva.12159","article-title":"Population-level effects of fitness costs associated with repressible female-lethal transgene insertions in two pest insects","volume":"7","author":"Harvey-Samuel","year":"2014","journal-title":"Evol. Appl."},{"key":"10.1016\/j.isci.2026.117402_bib124","doi-asserted-by":"crossref","DOI":"10.1126\/science.abj7484","article-title":"Direct observation of adaptive tracking on ecological time scales in Drosophila","volume":"375","author":"Rudman","year":"2022","journal-title":"Science"},{"key":"10.1016\/j.isci.2026.117402_bib125","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1093\/jme\/tjad015","article-title":"A Globally Distributed Insecticide Resistance Allele Confers a Fitness Cost in the Absence of Insecticide in Aedes aegypti (Diptera: Culicidae), the Yellow Fever Mosquito","volume":"60","author":"Fisher","year":"2023","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.isci.2026.117402_bib126","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s10340-023-01605-9","article-title":"Fitness costs of pyrethroid resistance in the polyphagous pest mite, Halotydeus destructor, under field conditions","volume":"97","author":"Umina","year":"2024","journal-title":"J. Pest. Sci."},{"key":"10.1016\/j.isci.2026.117402_bib127","doi-asserted-by":"crossref","first-page":"10381","DOI":"10.1128\/MCB.24.23.10381-10389.2004","article-title":"The Mouse Genomic Instability Mutation chaos1 Is an Allele of Polq That Exhibits Genetic Interaction with Atm","volume":"24","author":"Shima","year":"2004","journal-title":"Mol. 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