{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:50:46Z","timestamp":1791597046874,"version":"4.3.4"},"reference-count":33,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Forschung, Technologie und Raumfahrt","doi-asserted-by":"publisher","award":["COMPRare EJP RD JTC-2022"],"award-info":[{"award-number":["COMPRare EJP RD JTC-2022"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.isci.2026.117463","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:15:27Z","timestamp":1788610527000},"page":"117463","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Carboxypeptidase M deficiency aggravates complement-induced lung injury"],"prefix":"10.1016","volume":"29","author":[{"given":"Fatimunnisa","family":"Qadri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chubin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Sch\u00fctz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Popova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Atocha","family":"Czaplinska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Zemechmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramin","family":"Greckl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Alenina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joao B.","family":"Pesquero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 F.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Bader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2026.117463_bib1","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/s41577-021-00634-8","article-title":"Crosstalk between the renin\u2013angiotensin, complement and kallikrein\u2013kinin systems in inflammation","volume":"22","author":"Bekassy","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.isci.2026.117463_bib2","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/s41577-023-00926-1","article-title":"A guide to complement biology, pathology and therapeutic opportunity","volume":"24","author":"Mastellos","year":"2024","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.isci.2026.117463_bib3","doi-asserted-by":"crossref","DOI":"10.1172\/JCI163105","article-title":"C5aR1 signaling triggers lung immunopathology in COVID-19 through neutrophil extracellular traps","volume":"133","author":"Silva","year":"2023","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.isci.2026.117463_bib4","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1016\/j.molimm.2009.04.027","article-title":"The role of the anaphylatoxins in health and disease","volume":"46","author":"Klos","year":"2009","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.isci.2026.117463_bib5","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1146\/annurev.immunol.23.021704.115835","article-title":"Role of C5a in inflammatory responses","volume":"23","author":"Guo","year":"2005","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.isci.2026.117463_bib6","doi-asserted-by":"crossref","first-page":"3542","DOI":"10.4049\/jimmunol.1403068","article-title":"Is the Complement Activation Product C3a a Proinflammatory Molecule? Re-evaluating the Evidence and the Myth","volume":"194","author":"Coulthard","year":"2015","journal-title":"J Immunol."},{"key":"10.1016\/j.isci.2026.117463_bib7","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1172\/JCI106462","article-title":"Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase","volume":"49","author":"Bokisch","year":"1970","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.isci.2026.117463_bib8","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1348-0421.2002.tb02669.x","article-title":"Inactivation of C3a and C5a octapeptides by carboxypeptidase R and carboxypeptidase N","volume":"46","author":"Campbell","year":"2002","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.isci.2026.117463_bib9","doi-asserted-by":"crossref","first-page":"7165","DOI":"10.1074\/jbc.C100714200","article-title":"The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg74","volume":"277","author":"Cain","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117463_bib10","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1124\/pr.111.005223","article-title":"International union of basic and clinical pharmacology. LXXXVII. complement peptide C5a, C4a, and C3a receptors","volume":"65","author":"Klos","year":"2013","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.isci.2026.117463_bib11","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0165-2478(99)00002-4","article-title":"C3a(desArg) does not bind to and signal through the human C3a receptor","volume":"67","author":"Wilken","year":"1999","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.isci.2026.117463_bib12","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.cca.2008.10.003","article-title":"Carboxypeptidase M: Multiple alliances and unknown partners","volume":"399","author":"Deiteren","year":"2009","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.isci.2026.117463_bib13","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.jmb.2004.02.058","article-title":"Crystal structure of human carboxypeptidase M, A membrane-bound enzyme that regulates peptide hormone activity","volume":"338","author":"Reverter","year":"2004","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.isci.2026.117463_bib14","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1016\/j.toxicon.2010.04.007","article-title":"Cobra venom factor: Structure, function, and humanization for therapeutic complement depletion","volume":"56","author":"Vogel","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.isci.2026.117463_bib15","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/978-1-4614-0106-3_9","article-title":"Role of C3, C5 and anaphylatoxin receptors in acute lung injury and in sepsis","volume":"946","author":"Bosmann","year":"2012","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.isci.2026.117463_bib16","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1172\/JCI118818","article-title":"Requirement and role of C5a in acute lung inflammatory injury in rats","volume":"98","author":"Mulligan","year":"1996","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.isci.2026.117463_bib17","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1016\/j.intimp.2006.03.002","article-title":"Complement inhibitors selectively attenuate injury following administration of cobra venom factor to rats","volume":"6","author":"Proctor","year":"2006","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.isci.2026.117463_bib18","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0003-2697(03)00252-5","article-title":"Electrochemiluminescence assay for basic carboxypeptidases: Inhibition of basic carboxypeptidases and activation of thrombin-activatable fibrinolysis inhibitor","volume":"319","author":"Mao","year":"2003","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.isci.2026.117463_bib19","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1165\/rcmb.2009-0210ST","article-title":"An official american thoracic society workshop report: Features and measurements of experimental acute lung injury in animals","volume":"44","author":"Matute-Bello","year":"2011","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1016\/j.isci.2026.117463_bib20","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.bbapap.2006.11.017","article-title":"The role of the S1 binding site of carboxypeptidase M in substrate specificity and turn-over","volume":"1774","author":"Deiteren","year":"2007","journal-title":"Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"},{"key":"10.1016\/j.isci.2026.117463_bib21","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1016\/S0021-9258(19)41837-1","article-title":"Purification and partial characterization of human and porcine C3a anaphylatoxin","volume":"250","author":"Hugli","year":"1975","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117463_bib22","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1111\/jth.12956","article-title":"Carboxypeptidase B2 deficiency reveals opposite effects of complement C3a and C5a in a murine polymicrobial sepsis model","volume":"13","author":"Shao","year":"2015","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.isci.2026.117463_bib23","doi-asserted-by":"crossref","first-page":"2990","DOI":"10.1016\/j.jtha.2025.05.036","article-title":"Deficiencies of carboxypeptidase N and carboxypeptidase B2 have opposite effects in a virulent mouse E. coli sepsis model","volume":"23","author":"Zhou","year":"2025","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.isci.2026.117463_bib24","doi-asserted-by":"crossref","first-page":"6533","DOI":"10.4049\/jimmunol.0804207","article-title":"Targeted Disruption of the Gene Encoding the Murine Small Subunit of Carboxypeptidase N (CPN1) Causes Susceptibility to C5a Anaphylatoxin-Mediated Shock","volume":"182","author":"Mueller-Ortiz","year":"2009","journal-title":"J Immunol"},{"key":"10.1016\/j.isci.2026.117463_bib25","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1111\/jth.13964","article-title":"Carboxypeptidase B2 and N play different roles in regulation of activated complements C3a and C5a in mice","volume":"16","author":"Morser","year":"2018","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.isci.2026.117463_bib26","first-page":"48","article-title":"Potentiation of the anaphylatoxins in vivo using an inhibitor of serum carboxypeptidase N (SCPN). I. Lethality and pathologic effects on pulmonary tissue","volume":"112","author":"Huey","year":"1983","journal-title":"Am J Pathol"},{"key":"10.1016\/j.isci.2026.117463_bib27","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/S0140-6736(23)01524-6","article-title":"Complement in human disease: approved and up-and-coming therapeutics","volume":"403","author":"West","year":"2024","journal-title":"Lancet"},{"key":"10.1016\/j.isci.2026.117463_bib28","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1080\/08916930600739456","article-title":"Complement in lung disease","volume":"39","author":"Sarma","year":"2006","journal-title":"Autoimmunity"},{"key":"10.1016\/j.isci.2026.117463_bib29","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1080\/14787210.2022.2150165","article-title":"The efficacy and safety of complement C5a inhibitors for patients with severe COVID-19: a systematic review and meta-analysis","volume":"21","author":"Tsai","year":"2023","journal-title":"Expert Rev. Anti. Infect. Ther."},{"key":"10.1016\/j.isci.2026.117463_bib30","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1016\/j.theriogenology.2003.08.006","article-title":"Efficiency of transgenic rat production is independent of transgene-construct and overnight embryo culture","volume":"61","author":"Popova","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.isci.2026.117463_bib31","doi-asserted-by":"crossref","first-page":"15083","DOI":"10.1016\/S0021-9258(18)77226-8","article-title":"Carboxypeptidase M in Madin-Darby canine kidney cells. Evidence that carboxypeptidase M has a phosphatidylinositol glycan anchor","volume":"265","author":"Deddish","year":"1990","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117463_bib32","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/978-1-4939-6887-9_25","article-title":"Mass spectrometric analysis of proteins","volume":"1586","author":"Chalk","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.isci.2026.117463_bib33","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226028415?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226028415?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:22:07Z","timestamp":1791595327000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004226028415"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S2589004226028415"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2026.117463","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Carboxypeptidase M deficiency aggravates complement-induced lung injury","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2026.117463","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117463"}}