{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T22:25:22Z","timestamp":1794349522282,"version":"4.9.0"},"reference-count":47,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010905","name":"Major Research Plan of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92049109"],"award-info":[{"award-number":["92049109"]}],"id":[{"id":"10.13039\/501100010905","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32000549"],"award-info":[{"award-number":["32000549"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82003024"],"award-info":[{"award-number":["82003024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370629"],"award-info":[{"award-number":["32370629"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013058","name":"Jiangsu Provincial Key Research and Development Program","doi-asserted-by":"publisher","award":["BE20230804"],"award-info":[{"award-number":["BE20230804"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.isci.2026.117540","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:14:36Z","timestamp":1790349276000},"page":"117540","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification"],"prefix":"10.1016","volume":"29","author":[{"given":"Danping","family":"Guan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8763-2139","authenticated-orcid":false,"given":"Xiaoshan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixi","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinyao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuqing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomin","family":"Bu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanting","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruizhi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoubin","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoli","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoju","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2338-8076","authenticated-orcid":false,"given":"Shengkai","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyi","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2026.117540_bib1","article-title":"Cancer statistics, 2026","volume":"76","author":"Siegel","year":"2026","journal-title":"CA Cancer J. Clin."},{"key":"10.1016\/j.isci.2026.117540_bib2","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1038\/s41575-019-0189-8","article-title":"Global burden of colorectal cancer: emerging trends, risk factors and prevention strategies","volume":"16","author":"Keum","year":"2019","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.isci.2026.117540_bib3","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1186\/s12885-020-07074-y","article-title":"Impact of the faecal immunochemical test on colorectal cancer survival","volume":"20","author":"Gutierrez-Stampa","year":"2020","journal-title":"Bmc Cancer"},{"key":"10.1016\/j.isci.2026.117540_bib4","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/s41575-022-00612-y","article-title":"Current and future colorectal cancer screening strategies","volume":"19","author":"Shaukat","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.isci.2026.117540_bib5","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1056\/NEJMoa2208375","article-title":"Effect of Colonoscopy Screening on Risks of Colorectal Cancer and Related Death","volume":"387","author":"Bretthauer","year":"2022","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.isci.2026.117540_bib6","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.gastro.2005.05.056","article-title":"A comparison of the immunochemical fecal occult blood test and total colonoscopy in the asymptomatic population","volume":"129","author":"Morikawa","year":"2005","journal-title":"Gastroenterology"},{"key":"10.1016\/j.isci.2026.117540_bib7","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1001\/jama.2013.285718","article-title":"Effect of 3 to 5 Years of Scheduled CEA and CT Follow-up to Detect Recurrence of Colorectal Cancer The FACS Randomized Clinical Trial","volume":"311","author":"Primrose","year":"2014","journal-title":"Jama-Journal of the American Medical Association"},{"key":"10.1016\/j.isci.2026.117540_bib8","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1038\/cr.2008.282","article-title":"Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases","volume":"18","author":"Chen","year":"2008","journal-title":"Cell Res."},{"key":"10.1016\/j.isci.2026.117540_bib9","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/s41556-022-00880-5","article-title":"Exploring the expanding universe of small RNAs","volume":"24","author":"Shi","year":"2022","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.isci.2026.117540_bib10","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1093\/jmcb\/mjt052","article-title":"Identification and characterization of an ancient class of small RNAs enriched in serum associating with active infection","volume":"6","author":"Zhang","year":"2014","journal-title":"J. Mol. Cell Biol."},{"key":"10.1016\/j.isci.2026.117540_bib11","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1186\/s12943-020-01280-9","article-title":"Peripheral blood non-canonical small non-coding RNAs as novel biomarkers in lung cancer","volume":"19","author":"Gu","year":"2020","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.isci.2026.117540_bib12","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/nrc1997","article-title":"MicroRNA signatures in human cancers","volume":"6","author":"Calin","year":"2006","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.isci.2026.117540_bib13","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1200\/JCO.2009.24.9342","article-title":"Serum MicroRNA Signatures Identified in a Genome-Wide Serum MicroRNA Expression Profiling Predict Survival of Non-Small-Cell Lung Cancer","volume":"28","author":"Hu","year":"2010","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.isci.2026.117540_bib14","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.7150\/thno.80054","article-title":"Human circulating small non-coding RNA signature as a non-invasive biomarker in clinical diagnosis of acute myeloid leukaemia","volume":"13","author":"Xia","year":"2023","journal-title":"Theranostics"},{"key":"10.1016\/j.isci.2026.117540_bib15","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/s41574-024-01005-8","article-title":"Circulating non-coding RNA biomarkers of endocrine tumours","volume":"20","author":"Butz","year":"2024","journal-title":"Nat. Rev. Endocrinol."},{"key":"10.1016\/j.isci.2026.117540_bib16","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/s12943-021-01389-5","article-title":"A novel class of tsRNA signatures as biomarkers for diagnosis and prognosis of pancreatic cancer","volume":"20","author":"Jin","year":"2021","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.isci.2026.117540_bib17","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13073-021-00833-x","article-title":"5\u2032-tRF-GlyGCC: a tRNA-derived small RNA as a novel biomarker for colorectal cancer diagnosis","volume":"13","author":"Wu","year":"2021","journal-title":"Genome Med."},{"key":"10.1016\/j.isci.2026.117540_bib18","doi-asserted-by":"crossref","first-page":"245","DOI":"10.7150\/thno.102650","article-title":"tRNA-derived small RNAs in disease immunity","volume":"15","author":"Jia","year":"2025","journal-title":"Theranostics"},{"key":"10.1016\/j.isci.2026.117540_bib19","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1093\/jmcb\/mjx016","article-title":"A sequence of 28S rRNA-derived small RNAs is enriched in mature sperm and various somatic tissues and possibly associates with inflammation","volume":"9","author":"Chu","year":"2017","journal-title":"J. Mol. Cell Biol."},{"key":"10.1016\/j.isci.2026.117540_bib20","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1186\/s12967-025-06190-2","article-title":"Peripheral blood microbiome signature and Mycobacterium tuberculosis-derived rsRNA as diagnostic biomarkers for tuberculosis in human","volume":"23","author":"Gu","year":"2025","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.isci.2026.117540_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.eclinm.2019.12.006","article-title":"A validated composite model to predict risk of curve progression in adolescent idiopathic scoliosis","volume":"18","author":"Zhang","year":"2020","journal-title":"Eclinicalmedicine"},{"key":"10.1016\/j.isci.2026.117540_bib22","article-title":"A Combination of Let-7d, Let-7g and Let-7i Serves as a Stable Reference for Normalization of Serum microRNAs","volume":"8","author":"Chen","year":"2013","journal-title":"Plos One"},{"key":"10.1016\/j.isci.2026.117540_bib23","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.ejca.2010.10.025","article-title":"A five-microRNA signature identified from genome-wide serum microRNA expression profiling serves as a fingerprint for gastric cancer diagnosis","volume":"47","author":"Liu","year":"2011","journal-title":"Eur. J. Cancer"},{"key":"10.1016\/j.isci.2026.117540_bib24","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1373\/clinchem.2011.172767","article-title":"Serum MicroRNA Expression Profile as a Biomarker in the Diagnosis and Prognosis of Pancreatic Cancer","volume":"58","author":"Liu","year":"2012","journal-title":"Clin. Chem."},{"key":"10.1016\/j.isci.2026.117540_bib25","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1373\/clinchem.2010.147553","article-title":"Expression Profile of MicroRNAs in Serum: A Fingerprint for Esophageal Squamous Cell Carcinoma","volume":"56","author":"Zhang","year":"2010","journal-title":"Clin. Chem."},{"key":"10.1016\/j.isci.2026.117540_bib26","doi-asserted-by":"crossref","first-page":"6033","DOI":"10.1038\/s41467-020-19865-9","article-title":"Quantification of purified endogenous miRNAs with high sensitivity and specificity","volume":"11","author":"Shin","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.117540_bib27","first-page":"2059","article-title":"Identification of MicroRNAs and Their Target Genes Related to the Accumulation of Anthocyanins in Litchi chinensis by High -Throughput Sequencing and Degradome Analysis","volume":"7","author":"Liu","year":"2016","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.isci.2026.117540_bib28","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1105\/tpc.108.061143","article-title":"Eleven golden rules of quantitative RT-PCR","volume":"20","author":"Udvardi","year":"2008","journal-title":"Plant Cell"},{"key":"10.1016\/j.isci.2026.117540_bib29","doi-asserted-by":"crossref","first-page":"2034","DOI":"10.1039\/C8SC04621B","article-title":"Linear-hairpin variable primer RT-qPCR for MicroRNA","volume":"10","author":"Lan","year":"2019","journal-title":"Chem. Sci."},{"key":"10.1016\/j.isci.2026.117540_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2021.103613","article-title":"Translating genomic exploration of the family Polyomaviridae into confident human polyomavirus detection","volume":"25","author":"Kamminga","year":"2022","journal-title":"Iscience"},{"key":"10.1016\/j.isci.2026.117540_bib31","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkw399","article-title":"Sensitive and specific miRNA detection method using SplintR Ligase","volume":"44","author":"Jin","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2026.117540_bib32","doi-asserted-by":"crossref","first-page":"3489","DOI":"10.1158\/1538-7445.AM2017-3489","article-title":"Abstract 3489: Noncoding RNA distribution in clear cell renal cell cancer: small RNA-seq data","volume":"77","author":"Koduru","year":"2017","journal-title":"Cancer Res."},{"key":"10.1016\/j.isci.2026.117540_bib33","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/j.juro.2011.04.110","article-title":"miRNA Profiling for Clear Cell Renal Cell Carcinoma: Biomarker Discovery and Identification of Potential Controls and Consequences of miRNA Dysregulation","volume":"186","author":"White","year":"2011","journal-title":"J. Urol."},{"key":"10.1016\/j.isci.2026.117540_bib34","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.jmoldx.2012.02.003","article-title":"The Clinical Utility of miR-21 as a Diagnostic and Prognostic Marker for Renal Cell Carcinoma","volume":"14","author":"Faragalla","year":"2012","journal-title":"J. Mol. Diagn."},{"key":"10.1016\/j.isci.2026.117540_bib35","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1016\/j.humpath.2014.01.013","article-title":"Clear cell papillary renal cell carcinoma: micro-RNA expression profiling and comparison with clear cell renal cell carcinoma and papillary renal cell carcinoma","volume":"45","author":"Munari","year":"2014","journal-title":"Hum. Pathol."},{"key":"10.1016\/j.isci.2026.117540_bib36","doi-asserted-by":"crossref","DOI":"10.1155\/2019\/3717683","article-title":"Altered Serum MicroRNA Profile May Serve as an Auxiliary Tool for Discriminating Aggressive Thyroid Carcinoma from Nonaggressive Thyroid Cancer and Benign Thyroid Nodules","volume":"2019","author":"Zhang","year":"2019","journal-title":"Dis. Markers"},{"key":"10.1016\/j.isci.2026.117540_bib37","first-page":"311","article-title":"MicroRNAome Genome: A Treasure for Cancer Diagnosis and Therapy","volume":"64","author":"Berindan-Neagoe","year":"2014","journal-title":"CA Cancer J. Clin."},{"key":"10.1016\/j.isci.2026.117540_bib38","doi-asserted-by":"crossref","first-page":"4141","DOI":"10.1039\/D0CS00609B","article-title":"Advances in multiplexed techniques for the detection and quantification of microRNAs","volume":"50","author":"Jet","year":"2021","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.isci.2026.117540_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2023.105225","article-title":"Emerging functional principles of tRNA-derived small RNAs and other regulatory small RNAs","volume":"299","author":"Chen","year":"2023","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.isci.2026.117540_bib40","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2319569121","article-title":"The tRNA(Val) half: A strong endogenous Toll-like receptor 7 ligand with a 5'-terminal universal sequence signature","volume":"121","author":"Pawar","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.isci.2026.117540_bib41","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.tibs.2025.01.004","article-title":"Small RNA and Toll-like receptor interactions: origins and disease mechanisms","volume":"50","author":"Yu","year":"2025","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.isci.2026.117540_bib42","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s10020-025-01412-1","article-title":"Dissecting the small RNA code of inflammatory bowel disease","volume":"32","author":"Plank","year":"2026","journal-title":"Mol. Med."},{"key":"10.1016\/j.isci.2026.117540_bib43","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1128\/JCM.00167-14","article-title":"Rapid Genome Detection of Schmallenberg Virus and Bovine Viral Diarrhea Virus by Use of Isothermal Amplification Methods and High-Speed Real-Time Reverse Transcriptase PCR","volume":"52","author":"Aebischer","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.isci.2026.117540_bib44","doi-asserted-by":"crossref","first-page":"3665","DOI":"10.1007\/s11033-012-2442-x","article-title":"Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis","volume":"40","author":"Mohammadi-Yeganeh","year":"2013","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.isci.2026.117540_bib45","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gni178","article-title":"Real-time quantification of microRNAs by stem-loop RT-PCR - art. no. e179","volume":"33","author":"Chen","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.isci.2026.117540_bib46","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1586\/erd.10.22","article-title":"Recent advances in the development of nucleic acid diagnostics","volume":"7","author":"O'Connor","year":"2010","journal-title":"Expert Rev. Med. Devices"},{"key":"10.1016\/j.isci.2026.117540_bib47","doi-asserted-by":"crossref","first-page":"5487","DOI":"10.1039\/b509522k","article-title":"Fluorescence based strategies for genetic analysis","author":"Ranasinghe","year":"2005","journal-title":"Chem. Commun."}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226029196?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226029196?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T22:01:17Z","timestamp":1794348077000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004226029196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":47,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["S2589004226029196"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2026.117540","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2026.117540","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117540"}}