{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T11:31:17Z","timestamp":1794483077630,"version":"4.9.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013290","name":"National Key Research and Development Program of China Stem Cell and Translational Research","doi-asserted-by":"publisher","award":["2022YFE0138500"],"award-info":[{"award-number":["2022YFE0138500"]}],"id":[{"id":"10.13039\/501100013290","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013290","name":"National Key Research and Development Program of China Stem Cell and Translational Research","doi-asserted-by":"publisher","award":["2017ZX106080"],"award-info":[{"award-number":["2017ZX106080"]}],"id":[{"id":"10.13039\/501100013290","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["iScience"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.isci.2026.117857","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T08:53:51Z","timestamp":1791881631000},"page":"117857","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["A dual recombinant strategy to quantify PreS1 antibody in hepatitis B patients with genotype B or C virus by ELISA"],"prefix":"10.1016","volume":"29","author":[{"given":"Yumin","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumei","family":"Chi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanyi","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingrui","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqi","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7075-0331","authenticated-orcid":false,"given":"Zhenning","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.isci.2026.117857_bib1","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s12985-017-0814-5","article-title":"Association of the tandem polymorphisms (rs148314165, rs200820567) in TNFAIP3 with chronic hepatitis B virus infection in Chinese Han population","volume":"14","author":"Li","year":"2017","journal-title":"Virol. J."},{"key":"10.1016\/j.isci.2026.117857_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.microc.2021.106155","article-title":"Magnetic separation and enzymatic catalysis conjugated colorimetric immunosensor for Hepatitis B surface antigen detection","volume":"168","author":"Hu","year":"2021","journal-title":"Microchem. J."},{"key":"10.1016\/j.isci.2026.117857_bib3","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.661204","article-title":"The epigenetic modulation of cancer and immune pathways in hepatitis B virus-associated hepatocellular carcinoma: the influence of HBx and miRNA dysregulation","volume":"12","author":"Sartorius","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.isci.2026.117857_bib4","doi-asserted-by":"crossref","DOI":"10.1097\/HC9.0000000000000364","article-title":"Single-cell landscape identifies the immunophenotypes and microenvironments of HBV-positive and HBV-negative liver cancer","volume":"8","author":"Zheng","year":"2024","journal-title":"Hepatol. Commun."},{"key":"10.1016\/j.isci.2026.117857_bib5","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.canlet.2017.04.017","article-title":"Hepatitis B virus PreS1 facilitates hepatocellular carcinoma development by promoting appearance and self-renewal of liver cancer stem cells","volume":"400","author":"Liu","year":"2017","journal-title":"Cancer Lett."},{"key":"10.1016\/j.isci.2026.117857_bib6","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1002\/hep.26181","article-title":"Myristoylated preS1-domain of the hepatitis B virus L-protein mediates specific binding to differentiated hepatocytes","volume":"58","author":"Meier","year":"2013","journal-title":"Hepatology"},{"key":"10.1016\/j.isci.2026.117857_bib7","doi-asserted-by":"crossref","first-page":"9241","DOI":"10.1038\/s41467-024-53533-6","article-title":"Structural basis for hepatitis B virus restriction by a viral receptor homologue","volume":"15","author":"Shionoya","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.117857_bib8","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1038\/s41467-023-39148-3","article-title":"Targeted viral adaptation generates a simian-tropic hepatitis B virus that infects marmoset cells","volume":"14","author":"Liu","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.isci.2026.117857_bib9","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1002\/hep.29239","article-title":"Vaccines targeting preS1 domain overcome immune tolerance in hepatitis B virus carrier mice","volume":"66","author":"Bian","year":"2017","journal-title":"Hepatology"},{"key":"10.1016\/j.isci.2026.117857_bib10","doi-asserted-by":"crossref","first-page":"331","DOI":"10.3390\/vaccines9040331","article-title":"Efficacy of birth dose vaccination in preventing mother-to-child transmission of hepatitis B: A randomized controlled trial comparing engerix-B and Sci-B-Vac","volume":"9","author":"Safadi","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.isci.2026.117857_bib11","doi-asserted-by":"crossref","first-page":"e01680-20","DOI":"10.1128\/JVI.01680-20","article-title":"Identification of two critical neutralizing epitopes in the receptor binding domain of hepatitis B virus preS1","volume":"95","author":"Yato","year":"2021","journal-title":"J. Virol."},{"key":"10.1016\/j.isci.2026.117857_bib12","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.jinf.2021.12.025","article-title":"Construction of an anti-hepatitis B virus preS1 antibody and usefulness of preS1 measurement for chronic hepatitis B patients: anti-HBV preS1 antibody","volume":"84","author":"Hatooka","year":"2022","journal-title":"J. Infect."},{"key":"10.1016\/j.isci.2026.117857_bib13","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1038\/s41594-023-01191-5","article-title":"Structural basis of hepatitis B virus receptor binding","volume":"31","author":"Asami","year":"2024","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.isci.2026.117857_bib14","doi-asserted-by":"crossref","first-page":"276","DOI":"10.3748\/wjg.v8.i2.276","article-title":"Detection of anti-preS1 antibodies for recovery of hepatitis B patients by immunoassay","volume":"8","author":"Wei","year":"2002","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.isci.2026.117857_bib15","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21020546","article-title":"PreS1 mutations alter the large HBsAg antigenicity of a hepatitis B virus strain isolated in bangladesh","volume":"21","author":"Hossain","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.isci.2026.117857_bib16","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1038\/s41565-020-0648-y","article-title":"Dual-targeting nanoparticle vaccine elicits a therapeutic antibody response against chronic hepatitis B","volume":"15","author":"Wang","year":"2020","journal-title":"Nat. Nanotechnol."},{"key":"10.1016\/j.isci.2026.117857_bib17","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/j.1440-1746.2007.05174.x","article-title":"Demonstration of an association between detection of IgG antibody reactivity towards the C-terminal region of the preS1 protein of hepatitis B virus and the capacity to respond to interferon therapy in chronic hepatitis B","volume":"23","author":"Hellstr\u00f6m","year":"2008","journal-title":"J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.isci.2026.117857_bib18","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1111\/j.1478-3231.2005.01139.x","article-title":"Changing disease burden of hepatocellular carcinoma in the Far East and Southeast Asia","volume":"25","author":"Kao","year":"2005","journal-title":"Liver Int."},{"key":"10.1016\/j.isci.2026.117857_bib19","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1093\/jnci\/djn243","article-title":"Associations between hepatitis B virus genotype and mutants and the risk of hepatocellular carcinoma","volume":"100","author":"Yang","year":"2008","journal-title":"J. Natl. Cancer Inst."},{"key":"10.1016\/j.isci.2026.117857_bib20","doi-asserted-by":"crossref","DOI":"10.1080\/21505594.2025.2500490","article-title":"Novel prognostic scoring models for hepatitis B virus-related acute-on-chronic liver failure: A comparison with classical models","volume":"16","author":"Guo","year":"2025","journal-title":"Virulence"},{"key":"10.1016\/j.isci.2026.117857_bib21","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1016\/j.jhep.2016.01.027","article-title":"Overview of hepatitis B viral replication and genetic variability","volume":"64","author":"Tong","year":"2016","journal-title":"J. Hepatol."},{"key":"10.1016\/j.isci.2026.117857_bib22","article-title":"Clinical isolates of hepatitis B virus genotype C have higher in vitro transmission efficiency than genotype B isolates","volume":"95","author":"Li","year":"2023","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.isci.2026.117857_bib23","article-title":"Current and future therapeutic options for chronic hepatitis D virus infection","volume":"14","author":"Pisaturo","year":"2024","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.isci.2026.117857_bib24","doi-asserted-by":"crossref","first-page":"7400","DOI":"10.3748\/wjg.v21.i24.7400","article-title":"Applications of human hepatitis B virus preS domain in bio- and nanotechnology","volume":"21","author":"Toita","year":"2015","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.isci.2026.117857_bib25","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.687785","article-title":"The genotype (A to H) dependent N-terminal sequence of HBV large surface protein affects viral replication, secretion and infectivity","volume":"12","author":"Ou","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.isci.2026.117857_bib26","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1038\/s41586-022-04857-0","article-title":"Structural insights into the HBV receptor and bile acid transporter NTCP","volume":"606","author":"Park","year":"2022","journal-title":"Nature"},{"key":"10.1016\/j.isci.2026.117857_bib27","doi-asserted-by":"crossref","first-page":"754","DOI":"10.3390\/vaccines9070754","article-title":"Epitope-paratope interaction of a neutralizing human anti-hepatitis B virus preS1 antibody that recognizes the receptor-binding motif","volume":"9","author":"Hong","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.isci.2026.117857_bib28","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01686-21","article-title":"Establishment of a monoclonal antibody against human NTCP that blocks hepatitis B virus infection","volume":"96","author":"Takemori","year":"2022","journal-title":"J. Virol."},{"key":"10.1016\/j.isci.2026.117857_bib29","article-title":"ELISA genotyping of hepatitis B virus in China with antibodies specific for genotypes B and C","volume":"14","author":"Li","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.isci.2026.117857_bib30","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1137086","article-title":"Rapid whole genome sequencing methods for RNA viruses","volume":"14","author":"Misu","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.isci.2026.117857_bib31","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1742-4690-6-55","article-title":"96 shRNAs designed for maximal coverage of HIV-1 variants","volume":"6","author":"McIntyre","year":"2009","journal-title":"Retrovirology"},{"key":"10.1016\/j.isci.2026.117857_bib32","doi-asserted-by":"crossref","first-page":"62","DOI":"10.3892\/etm.2022.11761","article-title":"Clinical characteristics of patients infected with novel coronavirus wild strain, Delta variant strain and Omicron variant strain in Quanzhou: A real-world study","volume":"25","author":"Zhang","year":"2023","journal-title":"Exp. Ther. Med."},{"key":"10.1016\/j.isci.2026.117857_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0237951","article-title":"Comparative study on three viral enrichment approaches based on RNA extraction for plant virus\/viroid detection using high-throughput sequencing","volume":"15","author":"Gaafar","year":"2020","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2026.117857_bib34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0037874","article-title":"Ultra-deep pyrosequencing detects conserved genomic sites and quantifies linkage of drug-resistant amino acid changes in the hepatitis B virus genome","volume":"7","author":"Rodriguez-Fr\u00edas","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.isci.2026.117857_bib35","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s13099-025-00719-y","article-title":"Mutational landscape of the surface antigen of hepatitis B virus in patients with hepatocellular carcinoma","volume":"17","author":"Bagasi","year":"2025","journal-title":"Gut Pathog."}],"container-title":["iScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226032384?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2589004226032384?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T10:30:46Z","timestamp":1794479446000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2589004226032384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":35,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["S2589004226032384"],"URL":"https:\/\/doi.org\/10.1016\/j.isci.2026.117857","relation":{},"ISSN":["2589-0042"],"issn-type":[{"value":"2589-0042","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A dual recombinant strategy to quantify PreS1 antibody in hepatitis B patients with genotype B or C virus by ELISA","name":"articletitle","label":"Article Title"},{"value":"iScience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.isci.2026.117857","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117857"}}