{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:07:08Z","timestamp":1791079628313,"version":"4.1.0"},"reference-count":39,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100004334","name":"Merck","doi-asserted-by":"publisher","award":["AI020241"],"award-info":[{"award-number":["AI020241"]}],"id":[{"id":"10.13039\/100004334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004334","name":"Merck","doi-asserted-by":"publisher","award":["AI051645"],"award-info":[{"award-number":["AI051645"]}],"id":[{"id":"10.13039\/100004334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004334","name":"Merck","doi-asserted-by":"publisher","award":["AI022571"],"award-info":[{"award-number":["AI022571"]}],"id":[{"id":"10.13039\/100004334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI020241"],"award-info":[{"award-number":["AI020241"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI051645"],"award-info":[{"award-number":["AI051645"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI022571"],"award-info":[{"award-number":["AI022571"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jacionline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Allergy and Clinical Immunology"],"published-print":{"date-parts":[[2010,2]]},"DOI":"10.1016\/j.jaci.2009.11.029","type":"journal-article","created":{"date-parts":[[2010,2,12]],"date-time":"2010-02-12T04:31:11Z","timestamp":1265949071000},"page":"477-482","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":76,"title":["Cysteinyl leukotrienes acting via granule membrane-expressed receptors elicit secretion from within cell-free human eosinophil granules"],"prefix":"10.1016","volume":"125","author":[{"given":"Josiane S.","family":"Neves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy L.","family":"Radke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter F.","family":"Weller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jaci.2009.11.029_bib1","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1056\/NEJMra071371","article-title":"Leukotrienes","volume":"357","author":"Peters-Golden","year":"2007","journal-title":"N Engl J Med"},{"key":"10.1016\/j.jaci.2009.11.029_bib2","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1100\/tsw.2007.185","article-title":"Cysteinyl-leukotriene receptors and cellular signals","volume":"7","author":"Rovati","year":"2007","journal-title":"Sci World J"},{"key":"10.1016\/j.jaci.2009.11.029_bib3","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/21658","article-title":"Characterization of the human cysteinyl leukotriene CysLT1 receptor","volume":"399","author":"Lynch","year":"1999","journal-title":"Nature"},{"key":"10.1016\/j.jaci.2009.11.029_bib4","doi-asserted-by":"crossref","first-page":"30531","DOI":"10.1074\/jbc.M003490200","article-title":"Characterization of the human cysteinyl leukotriene 2 receptor","volume":"275","author":"Heise","year":"2000","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jaci.2009.11.029_bib5","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0065-2776(08)01003-1","article-title":"Biology of the eosinophil","volume":"101","author":"Blanchard","year":"2009","journal-title":"Adv Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib6","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1146\/annurev.immunol.24.021605.090720","article-title":"The eosinophil","volume":"24","author":"Rothenberg","year":"2006","journal-title":"Annu Rev Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib7","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1067\/mai.2000.105712","article-title":"Mechanisms of eosinophil-associated inflammation","volume":"105","author":"Gleich","year":"2000","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib8","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0952-3278(03)00074-7","article-title":"Eosinophils and cysteinyl leukotrienes","volume":"69","author":"Bandeira-Melo","year":"2003","journal-title":"Prostaglandins Leukot Essent Fatty Acids"},{"key":"10.1016\/j.jaci.2009.11.029_bib9","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1002\/med.20071","article-title":"Cysteinyl-leukotrienes and their receptors in asthma and other inflammatory diseases: critical update and emerging trends","volume":"27","author":"Capra","year":"2007","journal-title":"Med Res Rev"},{"key":"10.1016\/j.jaci.2009.11.029_bib10","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.4049\/jimmunol.173.3.1503","article-title":"Cysteinyl leukotrienes and their receptors: cellular distribution and function in immune and inflammatory responses","volume":"173","author":"Kanaoka","year":"2004","journal-title":"J Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib11","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1158\/0008-5472.732.65.3","article-title":"A novel localization of the G-protein-coupled CysLT1 receptor in the nucleus of colorectal adenocarcinoma cells","volume":"65","author":"Nielsen","year":"2005","journal-title":"Cancer Res"},{"key":"10.1016\/j.jaci.2009.11.029_bib12","doi-asserted-by":"crossref","first-page":"3263","DOI":"10.1182\/blood-2007-07-100453","article-title":"CysLT2 receptors interact with CysLT1 receptors and down-modulate cysteinyl leukotriene dependent mitogenic responses of mast cells","volume":"110","author":"Jiang","year":"2007","journal-title":"Blood"},{"key":"10.1016\/j.jaci.2009.11.029_bib13","doi-asserted-by":"crossref","first-page":"3333","DOI":"10.1073\/pnas.0508946103","article-title":"Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion","volume":"103","author":"Spencer","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jaci.2009.11.029_bib14","doi-asserted-by":"crossref","first-page":"18478","DOI":"10.1073\/pnas.0804547105","article-title":"Eosinophil granules function extracellularly as receptor-mediated secretory organelles","volume":"105","author":"Neves","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jaci.2009.11.029_bib15","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.1164\/ajrccm.161.6.9906085","article-title":"New aspects of degranulation and fates of airway mucosal eosinophils","volume":"161","author":"Erjefalt","year":"2000","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1016\/j.jaci.2009.11.029_bib16","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1177\/000348940311200211","article-title":"Cytolysis of eosinophils in nasal secretions","volume":"112","author":"Watanabe","year":"2003","journal-title":"Ann Otol Rhinol Laryngol"},{"key":"10.1016\/j.jaci.2009.11.029_bib17","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S0091-6749(97)70031-9","article-title":"Dermal eosinophils in atopic dermatitis undergo cytolytic degeneration","volume":"99","author":"Cheng","year":"1997","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib18","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1007\/s004360050459","article-title":"Immunoelectron microscopic evidence for release of eosinophil granule matrix protein onto microfilariae of Onchocerca volvulus in the skin after exposure to amocarzine","volume":"84","author":"Gutierrez-Pena","year":"1998","journal-title":"Parasitol Res"},{"key":"10.1016\/j.jaci.2009.11.029_bib19","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.jaci.2006.10.016","article-title":"Esophageal remodeling in pediatric eosinophilic esophagitis","volume":"119","author":"Aceves","year":"2007","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib20","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1097\/00000372-199602000-00008","article-title":"Free eosinophil granules in urticaria: a correlation with the duration of wheals","volume":"18","author":"Toyoda","year":"1996","journal-title":"Am J Dermatopathol"},{"key":"10.1016\/j.jaci.2009.11.029_bib21","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1097\/MPG.0b013e31806ab384","article-title":"Esophageal subepithelial fibrosis in children with eosinophilic esophagitis","volume":"45","author":"Chehade","year":"2007","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"10.1016\/j.jaci.2009.11.029_bib22","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.jim.2009.03.006","article-title":"Subcellular fractionation of human eosinophils: isolation of functional specific granules on isoosmotic density gradients","volume":"344","author":"Neves","year":"2009","journal-title":"J Immunol Methods"},{"key":"10.1016\/j.jaci.2009.11.029_bib23","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1189\/jlb.0108058","article-title":"Human eosinophils constitutively express multiple Th1, Th2, and immunoregulatory cytokines that are secreted rapidly and differentially","volume":"85","author":"Spencer","year":"2009","journal-title":"J Leukoc Biol"},{"key":"10.1016\/j.jaci.2009.11.029_bib24","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.jaci.2009.04.020","article-title":"Leukotriene E(4): perspective on the forgotten mediator","volume":"124","author":"Lee","year":"2009","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib25","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1164\/ajrccm.164.8.2102033","article-title":"Inhaled leukotriene E(4), but not leukotriene D(4), increased airway inflammatory cells in subjects with atopic asthma","volume":"164","author":"Gauvreau","year":"2001","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1016\/j.jaci.2009.11.029_bib26","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.jaci.2009.05.046","article-title":"The leukotriene E(4) puzzle: finding the missing pieces and revealing the pathobiologic implications","volume":"124","author":"Austen","year":"2009","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib27","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.bbrc.2005.09.052","article-title":"Identification of endogenous surrogate ligands for human P2Y12 receptors by in silico and in vitro methods","volume":"337","author":"Nonaka","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.jaci.2009.11.029_bib28","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1189\/jlb.0505286","article-title":"Activation of human eosinophils via P2 receptors: novel findings and future perspectives","volume":"79","author":"Ferrari","year":"2006","journal-title":"J Leukoc Biol"},{"key":"10.1016\/j.jaci.2009.11.029_bib29","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/j.1476-5381.2009.00169.x","article-title":"G protein-coupled receptor hetero-dimerization: contribution to pharmacology and function","volume":"158","author":"Milligan","year":"2009","journal-title":"Br J Pharmacol"},{"key":"10.1016\/j.jaci.2009.11.029_bib30","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/1471-230X-9-32","article-title":"Montelukast in the treatment of duodenal eosinophilia in children with dyspepsia: effect on eosinophil density and activation in relation to pharmacokinetics","volume":"9","author":"Friesen","year":"2009","journal-title":"BMC Gastroenterol"},{"key":"10.1016\/j.jaci.2009.11.029_bib31","doi-asserted-by":"crossref","first-page":"351","DOI":"10.2165\/00044011-200626060-00006","article-title":"Montelukast decreases plasma endothelin-1 and serum eosinophil cationic protein levels in paediatric atopic asthma","volume":"26","author":"Kopriva","year":"2006","journal-title":"Clin Drug Investig"},{"key":"10.1016\/j.jaci.2009.11.029_bib32","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.1517\/14656566.8.13.2173","article-title":"Update on montelukast and its role in the treatment of asthma, allergic rhinitis and exercise-induced bronchoconstriction","volume":"8","author":"Storms","year":"2007","journal-title":"Expert Opin Pharmacother"},{"key":"10.1016\/j.jaci.2009.11.029_bib33","doi-asserted-by":"crossref","first-page":"4615","DOI":"10.1038\/sj.emboj.7601341","article-title":"The orphan receptor GPR17 identified as a new dual uracil nucleotides\/cysteinyl-leukotrienes receptor","volume":"25","author":"Ciana","year":"2006","journal-title":"EMBO J"},{"key":"10.1016\/j.jaci.2009.11.029_bib34","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.bcp.2005.10.003","article-title":"CysLT1 leukotriene receptor antagonists inhibit the effects of nucleotides acting at P2Y receptors","volume":"71","author":"Mamedova","year":"2005","journal-title":"Biochem Pharmacol"},{"key":"10.1016\/j.jaci.2009.11.029_bib35","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.jaci.2006.03.010","article-title":"Montelukast regulates eosinophil protease activity through a leukotriene-independent mechanism","volume":"118","author":"Langlois","year":"2006","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib36","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1084\/jem.186.6.909","article-title":"Eosinophil lipid bodies: specific, inducible intracellular sites for enhanced eicosanoid formation","volume":"186","author":"Bozza","year":"1997","journal-title":"J\u00a0Exp Med"},{"key":"10.1016\/j.jaci.2009.11.029_bib37","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.4049\/jimmunol.162.3.1669","article-title":"Decreased leukotriene C4 synthesis accompanies adherence-dependent nuclear import of 5-lipoxygenase in human blood eosinophils","volume":"162","author":"Brock","year":"1999","journal-title":"J Immunol"},{"key":"10.1016\/j.jaci.2009.11.029_bib38","doi-asserted-by":"crossref","first-page":"22779","DOI":"10.1074\/jbc.M101436200","article-title":"Extranuclear lipid bodies, elicited by CCR3-mediated signaling pathways, are the sites of chemokine-enhanced leukotriene C4 production in eosinophils and basophils","volume":"276","author":"Bandeira-Melo","year":"2001","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jaci.2009.11.029_bib39","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1084\/jem.20020516","article-title":"Intracrine cysteinyl leukotriene receptor-mediated signaling of eosinophil vesicular transport-mediated interleukin-4 secretion","volume":"196","author":"Bandeira-Melo","year":"2002","journal-title":"J Exp Med"}],"container-title":["Journal of Allergy and Clinical Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091674909017503?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091674909017503?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T08:21:10Z","timestamp":1758788470000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0091674909017503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["S0091674909017503"],"URL":"https:\/\/doi.org\/10.1016\/j.jaci.2009.11.029","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.2675956.2338055","asserted-by":"object"}]},"ISSN":["0091-6749"],"issn-type":[{"value":"0091-6749","type":"print"}],"subject":[],"published":{"date-parts":[[2010,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cysteinyl leukotrienes acting via granule membrane-expressed receptors elicit secretion from within cell-free human eosinophil granules","name":"articletitle","label":"Article Title"},{"value":"Journal of Allergy and Clinical Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jaci.2009.11.029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}