{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:19:28Z","timestamp":1790266768581,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jacionline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Allergy and Clinical Immunology"],"published-print":{"date-parts":[[2010,12]]},"DOI":"10.1016\/j.jaci.2010.07.029","type":"journal-article","created":{"date-parts":[[2010,9,27]],"date-time":"2010-09-27T01:35:23Z","timestamp":1285551323000},"page":"1226-1233.e1","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":53,"title":["Characterization of \u03b6-associated protein, 70 kd (ZAP70)\u2013deficient human lymphocytes"],"prefix":"10.1016","volume":"126","author":[{"given":"Chaim M.","family":"Roifman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harjit","family":"Dadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raz","family":"Somech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Nahum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel","family":"Sharfe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jaci.2010.07.029_bib1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/0092-8674(92)90598-7","article-title":"ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain","volume":"71","author":"Chan","year":"1992","journal-title":"Cell"},{"key":"10.1016\/j.jaci.2010.07.029_bib2","doi-asserted-by":"crossref","first-page":"12310","DOI":"10.1016\/S0021-9258(17)32717-5","article-title":"Molecular cloning of human Syk. A\u00a0B cell protein-tyrosine kinase associated with the surface immunoglobulin M-B cell receptor complex","volume":"269","author":"Law","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jaci.2010.07.029_bib3","doi-asserted-by":"crossref","first-page":"4434","DOI":"10.1128\/MCB.17.8.4434","article-title":"Regulation of T-cell antigen receptor signalling by Syk tyrosine protein kinase","volume":"17","author":"Latour","year":"1997","journal-title":"Mol Cell Biol"},{"key":"10.1016\/j.jaci.2010.07.029_bib4","doi-asserted-by":"crossref","first-page":"4758","DOI":"10.4049\/jimmunol.152.10.4758","article-title":"Differential expression of ZAP-70 and Syk protein tyrosine kinases, and the role of this family of protein tyrosine kinases in TCR signaling","volume":"152","author":"Chan","year":"1994","journal-title":"J Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib5","doi-asserted-by":"crossref","first-page":"11685","DOI":"10.1016\/S0021-9258(19)49749-4","article-title":"A tyrosine-phosphorylated 70-kDa protein binds a photoaffinity analogue of ATP and associates with both the zeta chain and CD3 components of the activated T cell antigen receptor","volume":"267","author":"Wange","year":"1992","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jaci.2010.07.029_bib6","doi-asserted-by":"crossref","first-page":"647","DOI":"10.4049\/jimmunol.144.2.647","article-title":"Stimulation via CD3 Ti but not CD2 induces rapid tyrosine phosphorylation of a 68-kDa protein in the human Jurkat T cell line","volume":"144","author":"Jin","year":"1990","journal-title":"J Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib7","doi-asserted-by":"crossref","first-page":"18730","DOI":"10.1074\/jbc.270.32.18730","article-title":"Activating and inhibitory mutations in adjacent tyrosines in the kinase domain of ZAP-70","volume":"270","author":"Wange","year":"1995","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jaci.2010.07.029_bib8","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/0092-8674(94)90368-9","article-title":"Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase","volume":"76","author":"Arpaia","year":"1994","journal-title":"Cell"},{"key":"10.1016\/j.jaci.2010.07.029_bib9","series-title":"Lymphocyte activation and signal transduction","article-title":"Zap-70: 15 years on and still an expanding world","author":"Sharfe","year":"2007"},{"key":"10.1016\/j.jaci.2010.07.029_bib10","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1084\/jem.170.6.2177","article-title":"Depletion of CD8+ cells in human thymic medulla results in selective immune deficiency","volume":"170","author":"Roifman","year":"1989","journal-title":"J Exp Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib11","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1084\/jem.20042524","article-title":"New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling","volume":"201","author":"Dik","year":"2007","journal-title":"J Exp Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib12","doi-asserted-by":"crossref","first-page":"e445","DOI":"10.1542\/peds.2005-0369","article-title":"Novel RAG1 Mutation in a Case of Severe Combined Immunodeficiency","volume":"116","author":"Zhang","year":"2005","journal-title":"Pediatrics"},{"key":"10.1016\/j.jaci.2010.07.029_bib13","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/j.jaci.2009.06.048","article-title":"Reduced central tolerance in Omenn syndrome leads to immature self reactive oligoclonal T cells","volume":"124","author":"Somech","year":"2009","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib14","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1016\/S0140-6736(00)02293-5","article-title":"Assessment of thymic output in adults after haematopoietic stem cell transplantation and prediction of T-cell reconstitution","volume":"355","author":"Douek","year":"2000","journal-title":"Lancet"},{"key":"10.1016\/j.jaci.2010.07.029_bib15","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1056\/NEJMoa031178","article-title":"Effect of CD3\u03b4 deficiency on Maturation of \u03b1\/\u03b2 and \u03b3\/\u03b4 T-cell lineages in severe combined immunodeficiency","volume":"349","author":"Dadi","year":"2003","journal-title":"N Engl J Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib16","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/83784","article-title":"Disruption of a new forkhead\/winged helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse","volume":"27","author":"Brunkow","year":"2001","journal-title":"Nat Genet"},{"key":"10.1016\/j.jaci.2010.07.029_bib17","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1146\/annurev.immunol.23.021704.115611","article-title":"The B7 family revisited","volume":"23","author":"Greenwald","year":"2005","journal-title":"Annu Rev Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib18","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.immuni.2006.07.011","article-title":"Transforming growth factor beta controls development, homeostasis, and tolerance of T cells by regulatory T cell dependant and independent mechanisms","volume":"25","author":"Li","year":"2006","journal-title":"Immunity"},{"key":"10.1016\/j.jaci.2010.07.029_bib19","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.immuni.2006.07.012","article-title":"Cellular mechanisms of fatal early-onset autoimmunity in mice with the T cell specific targeting of transforming growth factor-beta receptor","volume":"25","author":"Marie","year":"2006","journal-title":"Immunity"},{"key":"10.1016\/j.jaci.2010.07.029_bib20","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1084\/jem.194.9.1253","article-title":"Sensory adaptation in na\u00efve peripheral CD4 T cells","volume":"194","author":"Smith","year":"2001","journal-title":"J Exp Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib21","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1084\/jem.193.10.1179","article-title":"Dynamic tuning of T cell reactivity by self-peptide-major histocompatibility complex ligands","volume":"193","author":"Wong","year":"2001","journal-title":"J Exp Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib22","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1084\/jem.20051304","article-title":"Independent roles for IL-2 and GATA-3 in stimulating na\u00efve CD4+ T cells to generate a Th2- inducing cytokine environment","volume":"202","author":"Yamane","year":"2005","journal-title":"J Exp Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib23","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/S1074-7613(00)80671-8","article-title":"Inhibition of Th1 development mediated by GATA-3 through an IL-4 independent mechanism","volume":"9","author":"Ouyang","year":"1998","journal-title":"Immunity"},{"key":"10.1016\/j.jaci.2010.07.029_bib24","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/ni808","article-title":"TH cell differentiation is accompanied by dynamic changes in histone acetylation of cytokine genes","volume":"3","author":"Avni","year":"2002","journal-title":"Nat Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib25","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S1074-7613(01)00125-X","article-title":"Regulation of IL-4 gene expression by distal regulatory elements and GATA-3 at the chromatin level","volume":"14","author":"Lee","year":"2001","journal-title":"Immunity"},{"key":"10.1016\/j.jaci.2010.07.029_bib26","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1084\/jem.192.1.105","article-title":"GATA-3 induces T helper cell type 2 (Th2) cytokine expression and chromatin remodeling in committed Th1 cells","volume":"192","author":"Lee","year":"2000","journal-title":"J Exp Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib27","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1038\/ni1115","article-title":"Long-range intrachromosomal interactions in the T\u00a0helper type 2 cytokine locus","volume":"5","author":"Spilianakis","year":"2004","journal-title":"Nat Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib28","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.autrev.2006.06.001","article-title":"Transforming growth factor-\u03b2 signaling through the Smad proteins: role in systemic sclerosis","volume":"5","author":"Verrecchia","year":"2006","journal-title":"Autoimmun Rev"},{"key":"10.1016\/j.jaci.2010.07.029_bib29","doi-asserted-by":"crossref","first-page":"6526","DOI":"10.4049\/jimmunol.173.11.6526","article-title":"Cutting edge: TGF-\u03b2 signaling is required for the in vivo expansion and immunosuppressive capacity of regulatory CD4+ CD25+ T cells","volume":"173","author":"Huber","year":"2004","journal-title":"J Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib30","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1084\/jem.20030152","article-title":"Conversion of peripheral CD4+ CD25-na\u00efve T cells to CD4+ CD25+ regulatory T cells by TGF-\u03b2 induction of transcription factor Foxp3","volume":"198","author":"Chen","year":"2003","journal-title":"J Exp Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib31","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1084\/jem.170.6.2081","article-title":"Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones","volume":"170","author":"Florentino","year":"1989","journal-title":"J Exp Med"},{"key":"10.1016\/j.jaci.2010.07.029_bib32","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0092-8674(93)80068-P","article-title":"Interleukin-10-deficient mice develop chronic enterocolitis","volume":"75","author":"Kuhn","year":"1993","journal-title":"Cell"},{"key":"10.1016\/j.jaci.2010.07.029_bib33","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1038\/ni1539","article-title":"TGF-\u03b2 and IL-6 drive the production of IL-17 and IL-10 by T\u00a0cells and restrain T(H)-17 cell-mediated pathology","volume":"8","author":"McGeachy","year":"2007","journal-title":"Nat Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib34","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1038\/ni1537","article-title":"Interleukins 27 and 6 induce STAT3-mediated T cell production of interleukin 10","volume":"8","author":"Stumhofer","year":"2007","journal-title":"Nat Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib35","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/nature03724","article-title":"Cellular and genetic mechanisms of self tolerance and autoimmunity","volume":"435","author":"Goodnow","year":"2005","journal-title":"Nature"},{"key":"10.1016\/j.jaci.2010.07.029_bib36","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0065-2776(08)60402-2","article-title":"CD95 APO-1\/Fas-mediated apoptosis: live and let die","volume":"71","author":"Krammer","year":"1999","journal-title":"Adv Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib37","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1126\/science.7533326","article-title":"The Fas death factor","volume":"267","author":"Nagata","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.jaci.2010.07.029_bib38","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.iac.2010.03.001","article-title":"Hematopoietic stem cell transplantation for profound T-cell deficiency (combined immunodeficiency)","volume":"30","author":"Roifman","year":"2010","journal-title":"Immunol Allergy Clin North Am"},{"key":"10.1016\/j.jaci.2010.07.029_bib39","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/376435a0","article-title":"Essential role for ZAP70 in both positive and negative selection of thymocytes","volume":"376","author":"Negishi","year":"1995","journal-title":"Nature"},{"key":"10.1016\/j.jaci.2010.07.029_bib40","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.4049\/jimmunol.163.5.2610","article-title":"Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression","volume":"163","author":"Chu","year":"1999","journal-title":"J Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib41","doi-asserted-by":"crossref","first-page":"656","DOI":"10.4049\/jimmunol.166.1.656","article-title":"Distinct T cell developmental consequences in humans and mice expressing identical mutations in the DLAARN motif of ZAP70","volume":"166","author":"Elder","year":"2001","journal-title":"J Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib42","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/nature02119","article-title":"Altered thymic T cell selection due to a mutation of the Zap 70 gene causes autoimmune arthritis in mice","volume":"426","author":"Sakaguchi","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.jaci.2010.07.029_bib43","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s00431-008-0718-x","article-title":"Clinical heterogeneity can hamper the diagnosis of patients with ZAP 70 deficiency","volume":"168","author":"Turul","year":"2009","journal-title":"Eur J Pediatr"},{"key":"10.1016\/j.jaci.2010.07.029_bib44","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1046\/j.1365-2249.1999.00759.x","article-title":"Existence of activated and memory CD4+ T cells in peripheral blood and their skin infiltration in CD8 deficiency","volume":"115","author":"Katamura","year":"1999","journal-title":"Clin Exp Immunol"},{"key":"10.1016\/j.jaci.2010.07.029_bib45","doi-asserted-by":"crossref","first-page":"A390","DOI":"10.1016\/S0091-6749(96)81048-7","article-title":"A 3 yr old boy with ZAP-70 deficiency","volume":"97","author":"Parry","year":"1996","journal-title":"J Allergy Clin Immunol"}],"container-title":["Journal of Allergy and Clinical Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091674910011747?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091674910011747?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T08:23:56Z","timestamp":1758788636000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0091674910011747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["S0091674910011747"],"URL":"https:\/\/doi.org\/10.1016\/j.jaci.2010.07.029","relation":{},"ISSN":["0091-6749"],"issn-type":[{"value":"0091-6749","type":"print"}],"subject":[],"published":{"date-parts":[[2010,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of \u03b6-associated protein, 70 kd (ZAP70)\u2013deficient human lymphocytes","name":"articletitle","label":"Article Title"},{"value":"Journal of Allergy and Clinical Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jaci.2010.07.029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}