{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T06:52:08Z","timestamp":1761979928259,"version":"build-2065373602"},"reference-count":37,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001411","name":"ICMR","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of the American Society of Cytopathology"],"published-print":{"date-parts":[[2022,5]]},"DOI":"10.1016\/j.jasc.2022.01.001","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T01:17:31Z","timestamp":1642036651000},"page":"154-164","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Multiplex fluorescence in situ hybridization testing for anaplastic lymphoma kinase and c-ros oncogene 1 gene rearrangements on cytology smears in lung adenocarcinomas: comparative study with formalin-fixed paraffin-embedded sections"],"prefix":"10.1016","volume":"11","author":[{"given":"Aruna","family":"Nambirajan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deeksha","family":"Rana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Komal","family":"Samant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aswini","family":"Prabakaran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prabhat","family":"Malik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7589-537X","authenticated-orcid":false,"given":"Deepali","family":"Jain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jasc.2022.01.001_bib2","doi-asserted-by":"crossref","first-page":"321","DOI":"10.5858\/arpa.2017-0388-CP","volume":"142","author":"Lindeman","year":"2018","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.jasc.2022.01.001_bib3","series-title":"IASLC Atlas of ALK and ROS1 Testing in Lung Cancer","first-page":"25","article-title":"ALK testing with IHC","author":"Thunnissen","year":"2016"},{"key":"10.1016\/j.jasc.2022.01.001_bib4","first-page":"147","article-title":"ALK (D5F3) CDx: an immunohistochemistry assay to identify ALK-positive NSCLC patients","volume":"11","author":"Uruga","year":"2018","journal-title":"Pharmgenomics Pers Med"},{"key":"10.1016\/j.jasc.2022.01.001_bib5","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1016\/j.jtho.2016.07.012","article-title":"ALK immunohistochemistry in NSCLC: discordant staining can impact patient treatment regimen","volume":"11","author":"Ibrahim","year":"2016","journal-title":"J Thorac Oncol"},{"key":"10.1016\/j.jasc.2022.01.001_bib6","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.1158\/1078-0432.CCR-08-3248","article-title":"KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer","volume":"15","author":"Takeuchi","year":"2009","journal-title":"Clin Cancer Res"},{"key":"10.1016\/j.jasc.2022.01.001_bib7","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.jtho.2015.12.111","article-title":"ALK protein analysis by IHC staining after recent regulatory changes: a comparison of two widely used approaches, revision of the literature, and a new testing algorithm","volume":"11","author":"Marchetti","year":"2016","journal-title":"J Thorac Oncol"},{"key":"10.1016\/j.jasc.2022.01.001_bib8","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/cyt.12627","article-title":"Cell blocks in cytopathology: an update","volume":"29","author":"Nambirajan","year":"2018","journal-title":"Cytopathology"},{"key":"10.1016\/j.jasc.2022.01.001_bib9","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1002\/cncy.22137","article-title":"Immunocytochemistry for predictive biomarker testing in lung cancer cytology","volume":"127","author":"Jain","year":"2019","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib10","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.lungcan.2013.03.006","article-title":"Clinical application of immunocytochemical detection of ALK rearrangement on cytology slides for detection or screening of lung adenocarcinoma","volume":"80","author":"Tanaka","year":"2013","journal-title":"Lung Cancer"},{"key":"10.1016\/j.jasc.2022.01.001_bib11","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1159\/000430083","article-title":"Anaplastic lymphoma kinase immunocytochemistry on cell-transferred cytologic smears of lung adenocarcinoma","volume":"59","author":"Zhang","year":"2015","journal-title":"Acta Cytol"},{"key":"10.1016\/j.jasc.2022.01.001_bib12","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1002\/cncy.21983","article-title":"Detection of ROS1-positive non-small cell lung cancer on cytological specimens using immunocytochemistry","volume":"126","author":"Vlajnic","year":"2018","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib13","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.lungcan.2010.09.013","article-title":"Frequent ALK rearrangement and TTF-1\/p63 co-expression in lung adenocarcinoma with signet-ring cell component","volume":"72","author":"Yoshida","year":"2011","journal-title":"Lung Cancer"},{"key":"10.1016\/j.jasc.2022.01.001_bib14","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1097\/PAS.0b013e3182758fe6","article-title":"ROS1-rearranged lung cancer: a clinicopathologic and molecular study of 15 surgical cases","volume":"37","author":"Yoshida","year":"2013","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib15","series-title":"IASLC Atlas of ALK and ROS1 Testing in Lung Cancer","first-page":"41","article-title":"ALK testing with FISH","author":"Yoshida","year":"2016"},{"key":"10.1016\/j.jasc.2022.01.001_bib16","series-title":"IASLC Atlas of ALK and ROS1 Testing in Lung Cancer","first-page":"53","article-title":"ROS1 testing with FISH","author":"Varella-Garcia","year":"2016"},{"key":"10.1016\/j.jasc.2022.01.001_bib17","doi-asserted-by":"crossref","first-page":"e647","DOI":"10.1016\/j.cllc.2018.04.016","article-title":"Evaluation of a dual ALK\/ROS1 fluorescent in situ hybridization test in non-small-cell lung cancer","volume":"19","author":"Ginestet","year":"2018","journal-title":"Clin Lung Cancer"},{"key":"10.1016\/j.jasc.2022.01.001_bib18","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1136\/jclinpath-2019-206152","article-title":"Multiplex fluorescence in situ hybridisation to detect anaplastic lymphoma kinase and ROS proto-oncogene 1 receptor tyrosine kinase rearrangements in lung cancer cytological samples","volume":"73","author":"Zito Marino","year":"2020","journal-title":"J Clin Pathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib19","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/cncy.21286","article-title":"The use of stained cytologic direct smears for ALK gene rearrangement analysis of lung adenocarcinoma","volume":"121","author":"Betz","year":"2013","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib20","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s00428-012-1281-4","article-title":"EML4-ALK testing in non-small cell carcinomas of the lung: a review with recommendations","volume":"461","author":"Thunnissen","year":"2012","journal-title":"Virchows Arch"},{"key":"10.1016\/j.jasc.2022.01.001_bib21","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1002\/dc.23318","article-title":"ALK and ROS1 rearrangements tested by fluorescence in situ hybridization in cytological smears from advanced non-small cell lung cancer patients","volume":"43","author":"Bozzetti","year":"2015","journal-title":"Diagn Cytopathol"},{"volume":"Vol. 5","article-title":"Thoracic tumours","year":"2021","key":"10.1016\/j.jasc.2022.01.001_bib22"},{"key":"10.1016\/j.jasc.2022.01.001_bib23","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.jtho.2016.03.019","article-title":"A validation study for the use of ROS1 immunohistochemical staining in screening for ROS1 translocations in lung cancer","volume":"11","author":"Viola","year":"2016","journal-title":"J Thorac Oncol"},{"key":"10.1016\/j.jasc.2022.01.001_bib24","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1309\/F5ADJUWVD2WFL8J5","article-title":"Multitarget FISH analysis in the diagnosis of lung cancer","volume":"123","author":"Bubendorf","year":"2005","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib25","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1365-2303.2004.00210.x","article-title":"Assessing HER-2 status in fresh frozen and archival cytological samples obtained by fine needle aspiration cytology","volume":"15","author":"Tom\u00e1s","year":"2004","journal-title":"Cytopathology"},{"key":"10.1016\/j.jasc.2022.01.001_bib26","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1002\/dc.23088","article-title":"Assessment of Her-2\/neu status using immunocytochemistry and fluorescence in situ hybridization on fine-needle aspiration cytology smears: experience from a tertiary care centre in India","volume":"42","author":"Durgapal","year":"2014","journal-title":"Diagn Cytopathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib27","doi-asserted-by":"crossref","first-page":"S1160","DOI":"10.1016\/j.jtho.2021.08.616","article-title":"P59.27 complementary utility of combined ALK\/ROS1 FISH with immunohistochemistry for ALK\/ROS1 rearrangement testing in lung cancer","volume":"16","author":"Nambirajan","year":"2021","journal-title":"J Thorac Oncol"},{"key":"10.1016\/j.jasc.2022.01.001_bib28","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1111\/cyt.12941","article-title":"EGFR mutation testing in cytology smears of advanced non-small cell lung carcinomas: practical applications in low-resource laboratories","volume":"32","author":"Singh","year":"2021","journal-title":"Cytopathology"},{"key":"10.1016\/j.jasc.2022.01.001_bib29","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1159\/000479217","article-title":"Use of exfoliative specimens and fine-needle aspiration smears for mutation testing in lung adenocarcinoma","volume":"61","author":"Jain","year":"2017","journal-title":"Acta Cytol"},{"key":"10.1016\/j.jasc.2022.01.001_bib30","doi-asserted-by":"crossref","first-page":"933","DOI":"10.5858\/arpa.2020-0119-CP","volume":"144","author":"Roy-Chowdhuri","year":"2020","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.jasc.2022.01.001_bib31","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1097\/JTO.0000000000000104","article-title":"ALK status testing in non-small-cell lung carcinoma by FISH on ThinPrep slides with cytology material","volume":"9","author":"Minca","year":"2014","journal-title":"J Thorac Oncol"},{"key":"10.1016\/j.jasc.2022.01.001_bib32","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1002\/cncy.21418","article-title":"Anaplastic lymphoma kinase gene rearrangements in cytological samples of non-small cell lung cancer: comparison with histological assessment","volume":"122","author":"Proietti","year":"2014","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib33","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1111\/cyt.12060","article-title":"ALK rearrangements in EBUS-derived transbronchial needle aspiration cytology in lung cancer","volume":"24","author":"Neat","year":"2013","journal-title":"Cytopathology"},{"key":"10.1016\/j.jasc.2022.01.001_bib34","doi-asserted-by":"crossref","first-page":"4486","DOI":"10.1002\/cncr.27411","article-title":"Correlations between the percentage of tumor cells showing an anaplastic lymphoma kinase (ALK) gene rearrangement, ALK signal copy number, and response to crizotinib therapy in ALK fluorescence in situ hybridization-positive non small cell lung cancer","volume":"118","author":"Camidge","year":"2012","journal-title":"Cancer"},{"key":"10.1016\/j.jasc.2022.01.001_bib35","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/cyt.12237","article-title":"ALK translocation detection in non-small cell lung cancer cytological samples obtained by TBNA or EBUS-TBNA","volume":"27","author":"Bravaccini","year":"2016","journal-title":"Cytopathology"},{"key":"10.1016\/j.jasc.2022.01.001_bib36","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1002\/cncy.21835","article-title":"Diagnosis of anaplastic lymphoma kinase rearrangement in cytological samples through a fluorescence in situ hybridization-based assay: Cytological smears versus cell blocks","volume":"125","author":"Zito Marino","year":"2017","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.01.001_bib37","doi-asserted-by":"crossref","first-page":"291","DOI":"10.5858\/arpa.2017-0208-RA","article-title":"Cytology smears in the era of molecular biomarkers in non-small cell lung cancer: doing more with less","volume":"142","author":"Lozano","year":"2018","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.jasc.2022.01.001_bib38","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1159\/000339792","article-title":"Role of fluorescence in situ hybridization in lung cancer cytology","volume":"56","author":"Savic","year":"2012","journal-title":"Acta Cytol"}],"container-title":["Journal of the American Society of Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213294522000011?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213294522000011?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T06:47:10Z","timestamp":1761979630000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2213294522000011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["S2213294522000011"],"URL":"https:\/\/doi.org\/10.1016\/j.jasc.2022.01.001","relation":{},"ISSN":["2213-2945"],"issn-type":[{"type":"print","value":"2213-2945"}],"subject":[],"published":{"date-parts":[[2022,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multiplex fluorescence in situ hybridization testing for anaplastic lymphoma kinase and c-ros oncogene 1 gene rearrangements on cytology smears in lung adenocarcinomas: comparative study with formalin-fixed paraffin-embedded sections","name":"articletitle","label":"Article Title"},{"value":"Journal of the American Society of Cytopathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jasc.2022.01.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 American Society of Cytopathology. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}