{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T06:51:46Z","timestamp":1761979906818,"version":"build-2065373602"},"reference-count":43,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100009957","name":"Adhesives and Sealant Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009957","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of the American Society of Cytopathology"],"published-print":{"date-parts":[[2022,9]]},"DOI":"10.1016\/j.jasc.2022.05.003","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T21:45:08Z","timestamp":1655156708000},"page":"295-305","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["High-risk human papillomavirus testing in cytology aspiration samples from the head and neck part 1: a review of the literature on available testing options"],"prefix":"10.1016","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3943-635X","authenticated-orcid":false,"given":"Varsha","family":"Manucha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9820-632X","authenticated-orcid":false,"given":"Adebowale J.","family":"Adeniran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saeed","family":"Asiry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rana S.","family":"Hoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel N.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0740-3227","authenticated-orcid":false,"given":"Annemieke","family":"van Zante","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8822-5655","authenticated-orcid":false,"given":"Christopher J.","family":"VandenBussche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2848-3185","authenticated-orcid":false,"given":"Christopher C.","family":"Griffith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jasc.2022.05.003_bib1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.5858\/arpa.2017-0286-CP","article-title":"Human papillomavirus testing in head and neck carcinomas guideline from the college of American pathologists","volume":"142","author":"Lewis","year":"2018","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.jasc.2022.05.003_bib2","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.1200\/JCO.18.00684","article-title":"Human papillomavirus testing in head and neck carcinomas: ASCO clinical practice guideline endorsement of the college of American pathologists guideline","volume":"36","author":"Fakhry","year":"2018","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.jasc.2022.05.003_bib3","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.path.2018.04.002","article-title":"Head and Neck Cytopathology: Human Papillomavirus-Positive Carcinomas, Including Diagnostic Updates, Testing Modalities, and Recommendations","volume":"11","author":"Madrigal","year":"2018","journal-title":"Surg Pathol Clin"},{"key":"10.1016\/j.jasc.2022.05.003_bib4","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1002\/dc.23548","article-title":"Fine-needle aspiration of cervical lymph nodes yields adequate materials for accurate HPV testing in metastatic head and neck squamous cell carcinomas","volume":"44","author":"Hou","year":"2016","journal-title":"Diagn Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib5","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jasc.2015.06.002","article-title":"Ultrasonography-guided fine-needle aspiration with concurrent small core biopsy of neck masses and lymph nodes yields adequate material for HPV testing in head and neck squamous cell carcinomas","volume":"5","author":"Allison","year":"2016","journal-title":"J Am Soc Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib6","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1369\/0022155416649579","article-title":"A Buffered Alcohol-Based Fixative for Histomorphologic and Molecular Applications","volume":"64","author":"Perry","year":"2016","journal-title":"J Histochem Cytochem"},{"key":"10.1016\/j.jasc.2022.05.003_bib7","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1002\/cncy.22170","article-title":"CytoLyt fixation significantly inhibits MIB1 immunoreactivity whereas alternative Ki-67 clone 30-9 is not susceptible to the inhibition: Critical diagnostic implications","volume":"127","author":"Buonocore","year":"2019","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib8","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1097\/PAI.0000000000000082","article-title":"CytoLyt\u00ae fixation and decalcification pretreatments alter antigenicity in normal tissues compared with standard formalin fixation","volume":"23","author":"Gruchy","year":"2015","journal-title":"Appl Immunohistochem Mol Morphol"},{"key":"10.1016\/j.jasc.2022.05.003_bib9","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/cncy.20034","article-title":"Immunohistochemical detection of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 expression in breast carcinomas: comparison on CB, needle-core, and tissue block preparations","volume":"117","author":"Hanley","year":"2009","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib10","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1002\/cncy.22191","article-title":"Cytologic evaluation of p16 staining in head and neck squamous cell carcinoma in CytoLyt versus formalin-fixed material","volume":"127","author":"Buonocore","year":"2019","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib11","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.jasc.2020.09.012","article-title":"Cytological interpretation of p16 immunohistochemistry in head and neck carcinomas: does the choice of fixative matter?","volume":"10","author":"Gargano","year":"2021","journal-title":"J Am Soc Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib12","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1002\/cncy.22178","article-title":"Heterogeneity of p16 immunohistochemistry and increased sensitivity of RNA in situ hybridization in cytology specimens of HPV-related head and neck squamous cell carcinoma","volume":"127","author":"Wong","year":"2019","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib13","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/cyt.12911","article-title":"Correlation of p16 immunostaining in cell-blocks with corresponding tissue specimens for squamous cell carcinomas of the oropharynx","volume":"32","author":"Song","year":"2021","journal-title":"Cytopathology"},{"key":"10.1016\/j.jasc.2022.05.003_bib14","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1159\/000496158","article-title":"Current Status of p16 Immunohistochemistry and HPV Testing in Fine Needle Aspiration Specimens of the Head and Neck","volume":"64","author":"Jalaly","year":"2020","journal-title":"Acta Cytol"},{"key":"10.1016\/j.jasc.2022.05.003_bib15","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1002\/cncy.21600","article-title":"Correlation of p16 immunohistochemistry in FNA biopsies with corresponding tissue specimens in HPV-related squamous cell carcinomas of the oropharynx","volume":"123","author":"Jalaly","year":"2015","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib16","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.humpath.2016.04.002","article-title":"The utility of p16 immunostaining in fine needle aspiration in p16-positive head and neck squamous cell carcinoma","volume":"54","author":"Xu","year":"2016","journal-title":"Hum Pathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib17","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.jasc.2021.03.004","article-title":"ROC analysis of p16 expression in cell blocks of metastatic head and neck squamous cell carcinoma","volume":"10","author":"Wilson","year":"2021","journal-title":"J Am Soc Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib18","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.jasc.2021.06.008","article-title":"p16 immunostaining in fine-needle aspirations of the head and neck: determining the optimal positivity threshold in HPV-related squamous cell cancer","volume":"10","author":"Wang","year":"2021","journal-title":"J Am Soc Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib19","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1002\/cncy.22399","article-title":"Quantitative assessment of p16 expression in FNA specimens from head and neck squamous cell carcinoma and correlation with HPV status","volume":"129","author":"Abi-Raad","year":"2020","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib20","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jasc.2020.01.004","article-title":"Threshold for interpretation of p16 immunostaining in fine-needle aspirate specimens of metastatic oropharyngeal squamous cell carcinoma","volume":"9","author":"Manucha","year":"2020","journal-title":"J Am Soc Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib21","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1159\/000375148","article-title":"The fidelity of p16 staining as a surrogate marker of human papillomavirus status in fine-needle aspirates and core biopsies of neck node metastases: Implications for HPV testing protocols","volume":"59","author":"Holmes","year":"2015","journal-title":"Acta Cytol"},{"key":"10.1016\/j.jasc.2022.05.003_bib22","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1158\/1078-0432.CCR-06-1690","article-title":"Detection of human papillomavirus-16 in fine-needle aspirates to determine tumor origin in patients with metastatic squamous cell carcinoma of the head and neck","volume":"13","author":"Begum","year":"2007","journal-title":"Clin Cancer Res"},{"key":"10.1016\/j.jasc.2022.05.003_bib23","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1002\/dc.21293","article-title":"Assessing for primary oropharyngeal or nasopharyngeal squamous cell carcinoma from fine needle aspiration of cervical lymph node metastases","volume":"38","author":"Jannapureddy","year":"2010","journal-title":"Diagn Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib24","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1097\/PAS.0b013e318265fb2b","article-title":"Detection of transcriptionally active high-risk HPV in patients with head and neck squamous cell carcinoma as visualized by a novel E6\/E7 mRNA in situ hybridization method","volume":"36","author":"Bishop","year":"2012","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib25","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1097\/PAS.0b013e318220e59d","article-title":"High-risk human papillomavirus E6\/E7 mRNA detection by a novel in situ hybridization assay strongly correlates with p16 expression and patient outcomes in oropharyngeal squamous cell carcinoma","volume":"35","author":"Ukpo","year":"2011","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib26","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1007\/s00405-012-2039-y","article-title":"The clinical impact of p16 status in fine-needle aspirates of cervical lymph node metastasis of head and neck squamous cell carcinomas","volume":"270","author":"Jakscha","year":"2013","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"10.1016\/j.jasc.2022.05.003_bib27","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1002\/cncy.22156","article-title":"HPV testing through p16 immunocytochemistry in neck-mass FNA and its correlation with tissue samples","volume":"127","author":"Yang","year":"2019","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib28","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1002\/dc.24696","article-title":"Diagnostic utility of p16 immunocytochemistry in metastatic cervical lymph nodes in head and neck cancers","volume":"49","author":"Kapoor","year":"2021","journal-title":"Diagn Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib29","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1002\/cncr.21027","article-title":"Human papillomavirus genome detection by in situ hybridization in fine-needle aspirates of metastatic lesions from head and neck squamous cell carcinomas","volume":"105","author":"Umudum","year":"2005","journal-title":"Cancer"},{"key":"10.1016\/j.jasc.2022.05.003_bib30","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1002\/cncr.23348","article-title":"Detection of human papillomavirus-related squamous cell carcinoma cytologically and by in situ hybridization in fine-needle aspiration biopsies of cervical metastasis: A tool for identifying the site of an occult head and neck primary","volume":"114","author":"Zhang","year":"2008","journal-title":"Cancer"},{"key":"10.1016\/j.jasc.2022.05.003_bib31","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1002\/cncy.21753","article-title":"Validation study of HPV DNA detection from stained FNA smears by polymerase chain reaction: Improving the diagnostic workup of patients with a tumor on the neck","volume":"124","author":"Channir","year":"2016","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib32","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.jasc.2016.05.001","article-title":"Aptima HR-HPV testing from Diff-Quick-stained fine-needle aspiration smears of oropharyngeal squamous cell carcinoma","volume":"5","author":"Han","year":"2016","journal-title":"J Am Soc Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib33","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1002\/cncy.21375","article-title":"Cervista HPV assays for fine-needle aspiration specimens are a valid option for human papillomavirus testing in patients with oropharyngeal carcinoma","volume":"122","author":"Guo","year":"2014","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib34","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1002\/dc.24603","article-title":"Liquid phase human papillomavirus genotype analysis of aspirated metastatic head and neck squamous cell carcinoma: fine needle aspiration supernatant is a rich source of tumor DNA that can increase the diagnostic yield","volume":"49","author":"Hao","year":"2021","journal-title":"Diagn Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib35","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1002\/cncy.22258","article-title":"Determination of high-risk HPV status of head and neck squamous cell carcinoma using the Roche cobas HPV test on cytologic specimens and acellular supernatant fluid","volume":"128","author":"Wright","year":"2020","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pvr.2018.05.003","article-title":"HPV E6 oncoproteins and nucleic acids in neck lymph node fine needle aspirates and oral samples from patients with oropharyngeal squamous cell carcinoma","volume":"6","author":"Chernesky","year":"2018","journal-title":"Papillomavirus Res"},{"key":"10.1016\/j.jasc.2022.05.003_bib37","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s40463-017-0189-z","article-title":"Developing a new diagnostic algorithm for human papilloma virus associated oropharyngeal carcinoma: an investigation of HPV DNA assays","volume":"46","author":"Cohen","year":"2017","journal-title":"J Otolaryngol Head Neck Surg"},{"key":"10.1016\/j.jasc.2022.05.003_bib38","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1309\/AJCPCZA4PSZCFHQ4","article-title":"Detection and genotype of high-risk human papillomavirus in fine-needle aspirates of patients with metastatic squamous cell carcinoma is helpful in determining tumor origin","volume":"143","author":"Baldassarri","year":"2015","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib39","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1002\/cncy.21372","article-title":"Performance of the roche cobas 4800 high-risk human papillomavirus test in cytologic preparations of squamous cell carcinoma of the head and neck","volume":"122","author":"Kerr","year":"2014","journal-title":"Cancer Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib40","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1002\/dc.23278","article-title":"Hybrid Capture 2 human papillomavirus testing of fine needle aspiration cytology of head and neck squamous cell carcinomas","volume":"43","author":"Hakima","year":"2015","journal-title":"Diagn Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib41","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.jasc.2014.02.004","article-title":"Hybrid Capture 2 human papilloma virus testing for head and neck cytology specimens","volume":"3","author":"Chute","year":"2014","journal-title":"J Am Soc Cytopathol"},{"key":"10.1016\/j.jasc.2022.05.003_bib42","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.oraloncology.2014.02.011","article-title":"Human papillomavirus status of head and neck cancer as determined in cytologic specimens using the hybrid-capture 2 assay","volume":"50","author":"Smith","year":"2014","journal-title":"Oral Oncol"},{"key":"10.1016\/j.jasc.2022.05.003_bib43","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1159\/000335730","article-title":"Assessment of fine needle aspiration specimen adequacy for high-risk HPV detection and genotyping in oropharyngeal squamous cell carcinoma","volume":"56","author":"Solomides","year":"2012","journal-title":"Acta Cytol"}],"container-title":["Journal of the American Society of Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213294522000576?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213294522000576?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T06:46:55Z","timestamp":1761979615000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2213294522000576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["S2213294522000576"],"URL":"https:\/\/doi.org\/10.1016\/j.jasc.2022.05.003","relation":{},"ISSN":["2213-2945"],"issn-type":[{"type":"print","value":"2213-2945"}],"subject":[],"published":{"date-parts":[[2022,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-risk human papillomavirus testing in cytology aspiration samples from the head and neck part 1: a review of the literature on available testing options","name":"articletitle","label":"Article Title"},{"value":"Journal of the American Society of Cytopathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jasc.2022.05.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 American Society of Cytopathology. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}