{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:03:11Z","timestamp":1790744591070,"version":"4.1.0"},"reference-count":50,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T00:00:00Z","timestamp":1632096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1016\/j.jbc.2021.101224","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T23:16:49Z","timestamp":1632266209000},"page":"101224","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":36,"title":["Mitochondrial respiratory chain function promotes extracellular matrix integrity in cartilage"],"prefix":"10.1016","volume":"297","author":[{"given":"Kristina","family":"Bubb","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"Holzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janica L.","family":"Nolte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5846-6941","authenticated-orcid":false,"given":"Marcus","family":"Kr\u00fcger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0152-4394","authenticated-orcid":false,"given":"Richard","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Schl\u00f6tzer-Schrehardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Brinckmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janine","family":"Altm\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Aszodi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Fleischhauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9413-0310","authenticated-orcid":false,"given":"Hauke","family":"Clausen-Schaumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Probst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3923-0554","authenticated-orcid":false,"given":"Bent","family":"Brachvogel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2021.101224_bib1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41580-020-00306-w","article-title":"Crosstalk between mechanotransduction and metabolism","volume":"22","author":"Romani","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib2","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.jid.2017.08.019","article-title":"Imbalance of mitochondrial respiratory chain complexes in the epidermis induces severe skin inflammation","volume":"138","author":"Weiland","year":"2018","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.jbc.2021.101224_bib3","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/j.cmet.2015.04.005","article-title":"Mosaic deficiency in mitochondrial oxidative metabolism promotes cardiac arrhythmia during aging","volume":"21","author":"Baris","year":"2015","journal-title":"Cell Metab."},{"key":"10.1016\/j.jbc.2021.101224_bib4","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<145::AID-GENE14>3.0.CO;2-C","article-title":"Col2a1-directed expression of Cre recombinase in differentiating chondrocytes in transgenic mice","volume":"26","author":"Ovchinnikov","year":"2000","journal-title":"Genesis"},{"key":"10.1016\/j.jbc.2021.101224_bib5","first-page":"3562","article-title":"miR-322 stabilizes MEK1 expression to inhibit RAF\/MEK\/ERK pathway activation in cartilage","volume":"144","author":"Bluhm","year":"2017","journal-title":"Development"},{"key":"10.1016\/j.jbc.2021.101224_bib6","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1083\/jcb.201809056","article-title":"Respiratory chain inactivation links cartilage-mediated growth retardation to mitochondrial diseases","volume":"218","author":"Holzer","year":"2019","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/nmeth.2769","article-title":"The promise of single-cell sequencing","volume":"11","author":"Eberwine","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jbc.2021.101224_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.matbio.2019.12.004","article-title":"Single cell RNA-sequencing reveals cellular heterogeneity and trajectories of lineage specification during murine embryonic limb development","volume":"89","author":"Kelly","year":"2020","journal-title":"Matrix Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib9","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.stemcr.2019.08.006","article-title":"Lgr5 and Col22a1 mark progenitor cells in the lineage toward juvenile articular chondrocytes","volume":"13","author":"Feng","year":"2019","journal-title":"Stem Cell Rep."},{"key":"10.1016\/j.jbc.2021.101224_bib10","doi-asserted-by":"crossref","DOI":"10.1242\/dev.185777","article-title":"A\u00a0single cell transcriptional atlas of early synovial joint development","volume":"147","author":"Bian","year":"2020","journal-title":"Development"},{"key":"10.1016\/j.jbc.2021.101224_bib11","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1016\/j.celrep.2016.04.043","article-title":"Systematic reconstruction of molecular cascades regulating GP development using single-cell RNA-seq","volume":"15","author":"Li","year":"2016","journal-title":"Cell Rep."},{"key":"10.1016\/j.jbc.2021.101224_bib12","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1002\/jbmr.30","article-title":"Sorting of growth plate chondrocytes allows the isolation and characterization of cells of a defined differentiation status","volume":"25","author":"Belluoccio","year":"2010","journal-title":"J.\u00a0Bone Miner. Res."},{"key":"10.1016\/j.jbc.2021.101224_bib13","doi-asserted-by":"crossref","first-page":"22163","DOI":"10.1074\/jbc.M610994200","article-title":"Abnormal collagen fibrils in cartilage of matrilin-1\/matrilin-3-deficient mice","volume":"282","author":"Nicolae","year":"2007","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2021.101224_bib14","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.aar2566","article-title":"Thrombospondin-1 promotes matrix homeostasis by interacting with collagen and lysyl oxidase precursors and collagen cross-linking sites","volume":"11","author":"Rosini","year":"2018","journal-title":"Sci. Signal."},{"key":"10.1016\/j.jbc.2021.101224_bib15","doi-asserted-by":"crossref","first-page":"4112","DOI":"10.3390\/ijms21114112","article-title":"Ablation of the miRNA cluster 24 has profound effects on extracellular matrix protein abundance in cartilage","volume":"21","author":"Georgieva","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbc.2021.101224_bib16","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.matbio.2017.07.005","article-title":"Identification of a myofibroblast-specific expression signature in skin wounds","volume":"65","author":"Bergmeier","year":"2018","journal-title":"Matrix Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib17","doi-asserted-by":"crossref","first-page":"5086","DOI":"10.3390\/ijms20205086","article-title":"Gene expression profiling of the extracellular matrix signature in macrophages of different activation status: Relevance for skin wound healing","volume":"20","author":"Etich","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbc.2021.101224_bib18","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ymeth.2008.01.002","article-title":"Advances in collagen cross-link analysis","volume":"45","author":"Eyre","year":"2008","journal-title":"Methods"},{"key":"10.1016\/j.jbc.2021.101224_bib19","doi-asserted-by":"crossref","first-page":"5665","DOI":"10.1111\/jcmm.15227","article-title":"Osteoidosis leads to altered differentiation and function of osteoclasts","volume":"24","author":"Grunherz","year":"2020","journal-title":"J.\u00a0Cell. Mol. Med."},{"key":"10.1016\/j.jbc.2021.101224_bib20","first-page":"732","article-title":"Effect of different hydroxyapatite incorporation methods on the structural and biological properties of porous collagen scaffolds for bone repair","volume":"227","author":"Ryan","year":"2015","journal-title":"J.\u00a0Anat."},{"key":"10.1016\/j.jbc.2021.101224_bib21","doi-asserted-by":"crossref","first-page":"5707","DOI":"10.4049\/jimmunol.180.8.5707","article-title":"Production of type VI collagen by human macrophages: A new dimension in macrophage functional heterogeneity","volume":"180","author":"Schnoor","year":"2008","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.jbc.2021.101224_bib22","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.matbio.2014.08.008","article-title":"The biology of lubricin: Near frictionless joint motion","volume":"39","author":"Jay","year":"2014","journal-title":"Matrix Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib23","doi-asserted-by":"crossref","first-page":"37758","DOI":"10.1074\/jbc.M111.248039","article-title":"Cartilage intermediate layer protein 2 (CILP-2) is expressed in articular and meniscal cartilage and down-regulated in experimental osteoarthritis","volume":"286","author":"Bernardo","year":"2011","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2021.101224_bib24","doi-asserted-by":"crossref","first-page":"23463","DOI":"10.1074\/jbc.273.36.23463","article-title":"A\u00a0novel cartilage protein (CILP) present in the mid-zone of human articular cartilage increases with age","volume":"273","author":"Lorenzo","year":"1998","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2021.101224_bib25","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.ydbio.2017.04.006","article-title":"Cell origin, volume and arrangement are drivers of articular cartilage formation, morphogenesis and response to injury in mouse limbs","volume":"426","author":"Decker","year":"2017","journal-title":"Dev. Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib26","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1016\/j.joca.2020.03.015","article-title":"Collagen IX deficiency leads to premature vascularization and ossification of murine femoral heads through an imbalance of pro- and antiangiogenic factors","volume":"28","author":"Heilig","year":"2020","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.jbc.2021.101224_bib27","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.bone.2012.10.011","article-title":"Differential development of the distal and proximal femoral epiphysis and physis in mice","volume":"52","author":"Cole","year":"2013","journal-title":"Bone"},{"key":"10.1016\/j.jbc.2021.101224_bib28","doi-asserted-by":"crossref","first-page":"8687","DOI":"10.1038\/ncomms9687","article-title":"Characterizing noise structure in single-cell RNA-seq distinguishes genuine from technical stochastic allelic expression","volume":"6","author":"Kim","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2021.101224_bib29","doi-asserted-by":"crossref","first-page":"899","DOI":"10.4049\/jimmunol.1600502","article-title":"Stromelysin-2 (MMP10) moderates inflammation by controlling macrophage activation","volume":"197","author":"McMahan","year":"2016","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.jbc.2021.101224_bib30","doi-asserted-by":"crossref","first-page":"12059","DOI":"10.1038\/s41598-017-12129-5","article-title":"Glycogen synthase kinase-3beta inhibition links mitochondrial dysfunction, extracellular matrix remodelling and terminal differentiation in chondrocytes","volume":"7","author":"Guidotti","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbc.2021.101224_bib31","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S8756-3282(98)00064-7","article-title":"Stromelysin-1 (MMP-3) and stromelysin-2 (MMP-10) expression in developing human bone: Potential roles in skeletal development","volume":"23","author":"Bord","year":"1998","journal-title":"Bone"},{"key":"10.1016\/j.jbc.2021.101224_bib32","doi-asserted-by":"crossref","first-page":"3244","DOI":"10.1002\/art.22167","article-title":"Matrix metalloproteinase 10 promotion of collagenolysis via procollagenase activation: Implications for cartilage degradation in arthritis","volume":"54","author":"Barksby","year":"2006","journal-title":"Arthritis Rheum."},{"key":"10.1016\/j.jbc.2021.101224_bib33","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1002\/stem.1553","article-title":"MMP-10 is required for efficient muscle regeneration in mouse models of injury and muscular dystrophy","volume":"32","author":"Bobadilla","year":"2014","journal-title":"Stem Cells"},{"key":"10.1016\/j.jbc.2021.101224_bib34","doi-asserted-by":"crossref","first-page":"32167","DOI":"10.1074\/jbc.M003834200","article-title":"Thrombospondin type 1 repeats interact with matrix metalloproteinase 2. Regulation of metalloproteinase activity","volume":"275","author":"Bein","year":"2000","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2021.101224_bib35","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1002\/(SICI)1097-0177(199912)216:4\/5<434::AID-DVDY11>3.0.CO;2-X","article-title":"Mice lacking matrilin-1 (cartilage matrix protein) have alterations in type II collagen fibrillogenesis and fibril organization","volume":"216","author":"Huang","year":"1999","journal-title":"Dev. Dyn."},{"key":"10.1016\/j.jbc.2021.101224_bib36","doi-asserted-by":"crossref","first-page":"9896","DOI":"10.1073\/pnas.1919702117","article-title":"Matrix mechanotransduction mediated by thrombospondin-1\/integrin\/YAP in the vascular remodeling","volume":"117","author":"Yamashiro","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2021.101224_bib37","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.matbio.2014.01.012","article-title":"Current understanding of the thrombospondin-1 interactome","volume":"37","author":"Resovi","year":"2014","journal-title":"Matrix Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib38","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1089\/ten.tea.2010.0691","article-title":"Thrombospondin-1 prevents excessive ossification in cartilage repair tissue induced by osteogenic protein-1","volume":"17","author":"Gelse","year":"2011","journal-title":"Tissue Eng. Part A"},{"key":"10.1016\/j.jbc.2021.101224_bib39","doi-asserted-by":"crossref","first-page":"14301","DOI":"10.1074\/jbc.M113.529982","article-title":"Matrilin-1 is an inhibitor of neovascularization","volume":"289","author":"Foradori","year":"2014","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2021.101224_bib40","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a006627","article-title":"Molecular basis for the regulation of angiogenesis by thrombospondin-1 and -2","volume":"2","author":"Lawler","year":"2012","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.jbc.2021.101224_bib41","doi-asserted-by":"crossref","first-page":"296069","DOI":"10.1155\/2011\/296069","article-title":"Thrombospondin-1: Multiple paths to inflammation","volume":"2011","author":"Lopez-Dee","year":"2011","journal-title":"Mediators Inflamm."},{"key":"10.1016\/j.jbc.2021.101224_bib42","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/bs.mcb.2017.08.018","article-title":"Matrilins","volume":"143","author":"Paulsson","year":"2018","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib43","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0076-6879(96)64045-2","article-title":"Cytochemistry and immunocytochemistry of mitochondria in tissue sections","volume":"264","author":"Sciacco","year":"1996","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jbc.2021.101224_bib44","doi-asserted-by":"crossref","first-page":"3333","DOI":"10.1021\/acs.jproteome.8b00093","article-title":"Single muscle fiber proteomics reveals distinct protein changes in slow and fast fibers during muscle atrophy","volume":"17","author":"Lang","year":"2018","journal-title":"J.\u00a0Proteome Res."},{"key":"10.1016\/j.jbc.2021.101224_bib45","doi-asserted-by":"crossref","first-page":"12648","DOI":"10.1038\/s41598-018-31154-6","article-title":"Instant clue: A software suite for interactive data visualization and analysis","volume":"8","author":"Nolte","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbc.2021.101224_bib46","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.joca.2020.09.008","article-title":"The Matrilin-3 T298M mutation predisposes for post-traumatic osteoarthritis in a knock-in mouse model","volume":"29","author":"Seifer","year":"2021","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.jbc.2021.101224_bib47","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-59745-413-1_6","article-title":"Quantitative determination of collagen cross-links","volume":"522","author":"Avery","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jbc.2021.101224_bib48","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1038\/s41563-020-00894-0","article-title":"Basement membrane stiffness determines metastases formation","volume":"20","author":"Reuten","year":"2021","journal-title":"Nat. Mater."},{"key":"10.1016\/j.jbc.2021.101224_bib49","doi-asserted-by":"crossref","first-page":"115181","DOI":"10.1016\/j.bone.2019.115181","article-title":"Sensory neuropeptides are required for bone and cartilage homeostasis in a murine destabilization-induced osteoarthritis model","volume":"133","author":"Muschter","year":"2020","journal-title":"Bone"},{"key":"10.1016\/j.jbc.2021.101224_bib50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.matbio.2015.10.001","article-title":"Structural and mechanical properties of the proliferative zone of the developing murine growth plate cartilage assessed by atomic force microscopy","volume":"50","author":"Prein","year":"2016","journal-title":"Matrix Biol."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925821010279?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925821010279?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:09:02Z","timestamp":1758240542000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925821010279"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["S0021925821010279"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2021.101224","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2021,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mitochondrial respiratory chain function promotes extracellular matrix integrity in cartilage","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2021.101224","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"101224"}}