{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T03:41:03Z","timestamp":1776742863343,"version":"3.51.2"},"reference-count":26,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T00:00:00Z","timestamp":1645056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2022,4]]},"DOI":"10.1016\/j.jbc.2022.101746","type":"journal-article","created":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T15:57:43Z","timestamp":1645199863000},"page":"101746","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s"],"prefix":"10.1016","volume":"298","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7826-0185","authenticated-orcid":false,"given":"Jose A.","family":"Amaya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David C.","family":"Lamb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven L.","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0132-2410","authenticated-orcid":false,"given":"Patrick","family":"Caffrey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3471-9211","authenticated-orcid":false,"given":"Vidhi C.","family":"Murarka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas L.","family":"Poulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2022.101746_bib1","first-page":"122","article-title":"Amphotericin B: An update","volume":"53","author":"Abu-Salah","year":"1996","journal-title":"Br. J. Biomed. Sci."},{"key":"10.1016\/j.jbc.2022.101746_bib2","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/j.chembiol.2003.12.001","article-title":"Biosynthesis of deoxyamphotericins and deoxyamphoteronolides by engineered strains of Streptomyces nodosus","volume":"10","author":"Byrne","year":"2003","journal-title":"Chem. Biol."},{"key":"10.1016\/j.jbc.2022.101746_bib3","doi-asserted-by":"crossref","first-page":"3893","DOI":"10.1007\/s00253-016-7474-z","article-title":"Polyene macrolide biosynthesis in streptomycetes and related bacteria: Recent advances from genome sequencing and experimental studies","volume":"100","author":"Caffrey","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbc.2022.101746_bib4","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1126\/science.1235797","article-title":"Structural basis for effector control and redox partner recognition in cytochrome P450","volume":"340","author":"Tripathi","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.jbc.2022.101746_bib5","doi-asserted-by":"crossref","first-page":"3412","DOI":"10.1021\/bi100183g","article-title":"P450cam visits an open conformation in the absence of substrate","volume":"49","author":"Lee","year":"2010","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbc.2022.101746_bib6","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.chembiol.2010.05.026","article-title":"Structure of cytochrome P450 PimD suggests epoxidation of the polyene macrolide pimaricin occurs via a hydroperoxoferric intermediate","volume":"17","author":"Kells","year":"2010","journal-title":"Chem. Biol."},{"key":"10.1016\/j.jbc.2022.101746_bib7","doi-asserted-by":"crossref","first-page":"3919","DOI":"10.1021\/cr400415k","article-title":"Heme enzyme structure and function","volume":"114","author":"Poulos","year":"2014","journal-title":"Chem. Rev."},{"key":"10.1016\/j.jbc.2022.101746_bib8","doi-asserted-by":"crossref","first-page":"4260","DOI":"10.1016\/S0021-9258(17)41772-8","article-title":"A\u00a0role for Asp-251 in cytochrome P-450cam oxygen activation","volume":"269","author":"Gerber","year":"1994","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2022.101746_bib9","doi-asserted-by":"crossref","first-page":"31659","DOI":"10.1074\/jbc.M505261200","article-title":"Crystallographic study on the dioxygen complex of wild-type and mutant cytochrome P450cam. Implications for the dioxygen activation mechanism","volume":"280","author":"Nagano","year":"2005","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2022.101746_bib10","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1038\/nsb0796-632","article-title":"Substrate-assisted catalysis in cytochrome P450eryF","volume":"3","author":"Cupp-Vickery","year":"1996","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.jbc.2022.101746_bib11","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1002\/cctc.201402773","article-title":"Exploration of the substrate promiscuity of biosynthetic tailoring enzymes as a new source of structural diversity for polyene macrolide antifungals","volume":"7","author":"Santos-Aberturas","year":"2015","journal-title":"ChemCatChem"},{"key":"10.1016\/j.jbc.2022.101746_bib12","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1006\/jmbi.2000.4155","article-title":"How do substrates enter and products exit the buried active site of cytochrome P450cam? 2. Steered molecular dynamics and adiabatic mapping of substrate pathways","volume":"303","author":"Ludemann","year":"2000","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.jbc.2022.101746_bib13","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1103\/PhysRevLett.78.2690","article-title":"Nonequilibrium equality for free energy differences","volume":"78","author":"Jarzynski","year":"1997","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.jbc.2022.101746_bib14","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1128\/AEM.72.4.2514-2519.2006","article-title":"Characterization of the P450 monooxygenase NysL, responsible for C-10 hydroxylation during biosynthesis of the polyene macrolide antibiotic nystatin in Streptomyces noursei","volume":"72","author":"Volokhan","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jbc.2022.101746_bib15","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"XDS","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2022.101746_bib16","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1107\/S0907444906045975","article-title":"Solving structures of protein complexes by molecular replacement with Phaser","volume":"63","author":"McCoy","year":"2007","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2022.101746_bib17","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.jbc.2022.101746_bib18","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.ymeth.2011.07.005","article-title":"The Phenix software for automated determination of macromolecular structures","volume":"55","author":"Adams","year":"2011","journal-title":"Methods"},{"key":"10.1016\/j.jbc.2022.101746_bib19","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1107\/S0021889812017293","article-title":"Graphical tools for macromolecular crystallography in PHENIX","volume":"45","author":"Echols","year":"2012","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.jbc.2022.101746_bib20","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1002\/jcc.25187","article-title":"Fast and flexible gpu accelerated binding free energy calculations within the amber molecular dynamics package","volume":"39","author":"Mermelstein","year":"2018","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.jbc.2022.101746_bib21","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1002\/jcc.20035","article-title":"Development and testing of a general amber force field","volume":"25","author":"Wang","year":"2004","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.jbc.2022.101746_bib22","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1002\/jcc.10128","article-title":"Fast, efficient generation of high-quality atomic charges. AM1-BCC model: II. Parameterization and validation","volume":"23","author":"Jakalian","year":"2002","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.jbc.2022.101746_bib23","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/jcc.21922","article-title":"Quantum mechanically derived AMBER-compatible heme parameters for various states of the cytochrome P450 catalytic cycle","volume":"33","author":"Shahrokh","year":"2012","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.jbc.2022.101746_bib24","doi-asserted-by":"crossref","first-page":"5946","DOI":"10.1063\/1.1651473","article-title":"Calculating potentials of mean force from steered molecular dynamics simulations","volume":"120","author":"Park","year":"2004","journal-title":"J.\u00a0Chem. Phys."},{"key":"10.1016\/j.jbc.2022.101746_bib25","doi-asserted-by":"crossref","first-page":"5338","DOI":"10.1021\/acs.jpcb.0c02009","article-title":"How good is Jarzynski's equality for computer-aided drug design?","volume":"124","author":"Ho","year":"2020","journal-title":"J.\u00a0Phys. Chem. B"},{"key":"10.1016\/j.jbc.2022.101746_bib26","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.bpc.2016.01.003","article-title":"Steered molecular dynamics study of inhibitor binding in the internal binding site in dehaloperoxidase-hemoglobin","volume":"211","author":"Zhang","year":"2016","journal-title":"Biophys. Chem."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925822001867?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925822001867?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T03:14:08Z","timestamp":1720062848000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925822001867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["S0021925822001867"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2022.101746","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural analysis of P450 AmphL from Streptomyces nodosus provides insights into substrate selectivity of polyene macrolide antibiotic biosynthetic P450s","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2022.101746","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"101746"}}