{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:12:29Z","timestamp":1791616349641,"version":"4.3.4"},"reference-count":46,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,2,19]],"date-time":"2022-02-19T00:00:00Z","timestamp":1645228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2022,4]]},"DOI":"10.1016\/j.jbc.2022.101763","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:15:23Z","timestamp":1645488923000},"page":"101763","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"title":["Development of high-affinity nanobodies specific for NaV1.4 and NaV1.5 voltage-gated sodium channel isoforms"],"prefix":"10.1016","volume":"298","author":[{"given":"Lakshmi","family":"Srinivasan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanina","family":"Alzogaray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dakshnamurthy","family":"Selvakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Nathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse B.","family":"Yoder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharine M.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8777-0870","authenticated-orcid":false,"given":"Sebasti\u00e1n","family":"Klinke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5348-6196","authenticated-orcid":false,"given":"Justin N.","family":"Nwafor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda S.","family":"Labanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando A.","family":"Goldbaum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2015-2090","authenticated-orcid":false,"given":"Arne","family":"Sch\u00f6n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Freire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon F.","family":"Tomaselli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Mario","family":"Amzel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu","family":"Ben-Johny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra B.","family":"Gabelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2022.101763_bib1","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1152\/physrev.1996.76.3.887","article-title":"Structure and function of voltage-dependent sodium channels: Comparison of brain II and cardiac isoforms","volume":"76","author":"Fozzard","year":"1996","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.jbc.2022.101763_bib2","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1172\/JCI25505","article-title":"Inherited disorders of voltage-gated sodium channels","volume":"115","author":"George","year":"2005","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.jbc.2022.101763_bib3","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/74159","article-title":"Mutations of SCN1A, encoding a neuronal sodium channel, in two families with GEFS+2","volume":"24","author":"Escayg","year":"2000","journal-title":"Nat. Genet."},{"key":"10.1016\/j.jbc.2022.101763_bib4","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1086\/319516","article-title":"Neuronal sodium-channel alpha1-subunit mutations in generalized epilepsy with febrile seizures plus","volume":"68","author":"Wallace","year":"2001","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.jbc.2022.101763_bib5","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/s41573-019-0013-8","article-title":"Antibodies and venom peptides: New modalities for ion channels","volume":"18","author":"Wulff","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jbc.2022.101763_bib6","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1080\/19420862.2018.1548232","article-title":"Ion channels as therapeutic antibody targets","volume":"11","author":"Hutchings","year":"2019","journal-title":"MAbs"},{"key":"10.1016\/j.jbc.2022.101763_bib7","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1038\/s41467-020-14889-7","article-title":"Nanobody-enabled monitoring of kappa opioid receptor states","volume":"11","author":"Che","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2022.101763_bib8","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0167-4838(99)00030-8","article-title":"Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies","volume":"1431","author":"van der Linden","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jbc.2022.101763_bib9","doi-asserted-by":"crossref","first-page":"4586","DOI":"10.1073\/pnas.0505379103","article-title":"Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies","volume":"103","author":"De Genst","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2022.101763_bib10","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.coi.2018.02.003","article-title":"Modulating ion channel function with antibodies and nanobodies","volume":"52","author":"Stortelers","year":"2018","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.jbc.2022.101763_bib11","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1097\/j.pain.0000000000001587","article-title":"Antibodies binding the head domain of P2X4 inhibit channel function and reverse neuropathic pain","volume":"160","author":"Williams","year":"2019","journal-title":"Pain"},{"key":"10.1016\/j.jbc.2022.101763_bib12","doi-asserted-by":"crossref","first-page":"366ra162","DOI":"10.1126\/scitranslmed.aaf8463","article-title":"Nanobodies that block gating of the P2X7 ion channel ameliorate inflammation","volume":"8","author":"Danquah","year":"2016","journal-title":"Sci. translational Med."},{"key":"10.1016\/j.jbc.2022.101763_bib13","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.3389\/fimmu.2017.01234","article-title":"Unveiling a drift resistant cryptotope within marburgvirus nucleoprotein recognized by llama single-domain antibodies","volume":"8","author":"Garza","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jbc.2022.101763_bib14","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1107\/S2053230X17005969","article-title":"Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis","volume":"73","author":"Duhoo","year":"2017","journal-title":"Acta Crystallogr. F Struct. Biol. Commun."},{"key":"10.1016\/j.jbc.2022.101763_bib15","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1038\/ncomms3660","article-title":"Visualization and targeted disruption of protein interactions in living cells","volume":"4","author":"Herce","year":"2013","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2022.101763_bib16","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1038\/nmeth.1991","article-title":"A\u00a0simple, versatile method for GFP-based super-resolution microscopy via nanobodies","volume":"9","author":"Ries","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jbc.2022.101763_bib17","doi-asserted-by":"crossref","first-page":"E6227","DOI":"10.1073\/pnas.1801865115","article-title":"A\u00a0versatile nanobody-based toolkit to analyze retrograde transport from the cell surface","volume":"115","author":"Buser","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2022.101763_bib18","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1016\/j.jcmg.2018.07.027","article-title":"Nanobody-facilitated multiparametric PET\/MRI phenotyping of atherosclerosis","volume":"12","author":"Senders","year":"2019","journal-title":"JACC Cardiovasc. Imaging"},{"key":"10.1016\/j.jbc.2022.101763_bib19","article-title":"A\u00a0nanobody targeting viral nonstructural protein 9 inhibits porcine reproductive and respiratory syndrome virus replication","volume":"93","author":"Wang","year":"2019","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2022.101763_bib20","doi-asserted-by":"crossref","first-page":"4653","DOI":"10.1073\/pnas.1919409117","article-title":"A\u00a0nanobody targeting the LIN28:let-7 interaction fragment of TUT4 blocks uridylation of let-7","volume":"117","author":"Yu","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2022.101763_bib21","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nsmb.2180","article-title":"Fluorescent fusion protein knockout mediated by anti-GFP nanobody","volume":"19","author":"Caussinus","year":"2011","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.jbc.2022.101763_bib22","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02005-18","article-title":"Structural basis of nanobodies targeting the prototype norovirus","volume":"93","author":"Ruoff","year":"2019","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2022.101763_bib23","doi-asserted-by":"crossref","first-page":"4531","DOI":"10.1128\/JB.187.13.4531-4541.2005","article-title":"Llama antibodies against a lactococcal protein located at the tip of the phage tail prevent phage infection","volume":"187","author":"De Haard","year":"2005","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbc.2022.101763_bib24","doi-asserted-by":"crossref","first-page":"4838","DOI":"10.4049\/jimmunol.1600692","article-title":"Generation of immunity against pathogens via single-domain antibody-antigen constructs","volume":"197","author":"Duarte","year":"2016","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.jbc.2022.101763_bib25","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1056\/NEJMoa1806311","article-title":"Caplacizumab treatment for acquired thrombotic thrombocytopenic purpura","volume":"380","author":"Scully","year":"2019","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.jbc.2022.101763_bib26","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1128\/AAC.01802-15","article-title":"Generation and characterization of ALX-0171, a potent novel therapeutic nanobody for the treatment of respiratory syncytial virus infection","volume":"60","author":"Detalle","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jbc.2022.101763_bib27","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1039\/C3NR04878K","article-title":"Transport of nanoparticles through the blood-brain barrier for imaging and therapeutic applications","volume":"6","author":"Shilo","year":"2014","journal-title":"Nanoscale"},{"key":"10.1016\/j.jbc.2022.101763_bib28","doi-asserted-by":"crossref","first-page":"5126","DOI":"10.1038\/ncomms6126","article-title":"Regulation of the NaV1.5 cytoplasmic domain by calmodulin","volume":"5","author":"Gabelli","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2022.101763_bib29","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1038\/s41467-019-09570-7","article-title":"Ca(2+)-dependent regulation of sodium channels NaV1.4 and NaV1.5 is controlled by the post-IQ motif","volume":"10","author":"Yoder","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2022.101763_bib30","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1038\/nprot.2014.039","article-title":"A\u00a0general protocol for the generation of Nanobodies for structural biology","volume":"9","author":"Pardon","year":"2014","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jbc.2022.101763_bib31","article-title":"Inhibition of type VI secretion by an anti-TssM llama nanobody","volume":"10","author":"Nguyen","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.jbc.2022.101763_bib32","doi-asserted-by":"crossref","first-page":"581856","DOI":"10.3389\/fmicb.2020.581856","article-title":"Structural characterization of non-structural protein 9 complexed with specific nanobody pinpoints two important residues involved in porcine reproductive and respiratory syndrome virus replication","volume":"11","author":"Wang","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jbc.2022.101763_bib33","doi-asserted-by":"crossref","first-page":"114240","DOI":"10.1016\/j.ab.2021.114240","article-title":"Reversibility and irreversibility in the temperature denaturation of monoclonal antibodies","volume":"626","author":"Schon","year":"2021","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jbc.2022.101763_bib34","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/bs.mie.2021.01.047","article-title":"Probing ion channel macromolecular interactions using fluorescence resonance energy transfer","volume":"653","author":"Rivas","year":"2021","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jbc.2022.101763_bib35","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1021\/cr960383c","article-title":"Protein structure and the energetics of protein stability","volume":"97","author":"Robertson","year":"1997","journal-title":"Chem. Rev."},{"key":"10.1016\/j.jbc.2022.101763_bib36","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1002\/prot.25354","article-title":"Temperature stability of proteins: Analysis of irreversible denaturation using isothermal calorimetry","volume":"85","author":"Schon","year":"2017","journal-title":"Proteins"},{"key":"10.1016\/j.jbc.2022.101763_bib37","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.49253","article-title":"A\u00a0potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVbeta subunits","volume":"8","author":"Morgenstern","year":"2019","journal-title":"eLife"},{"key":"10.1016\/j.jbc.2022.101763_bib38","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jbiotec.2019.01.001","article-title":"Characterization of folding-sensitive nanobodies as tools to study the expression and quality of protein particle immunogens","volume":"293","author":"Alzogaray","year":"2019","journal-title":"J.\u00a0Biotechnol."},{"key":"10.1016\/j.jbc.2022.101763_bib39","doi-asserted-by":"crossref","first-page":"W636","DOI":"10.1093\/nar\/gkz268","article-title":"The EMBL-EBI search and sequence analysis tools APIs in 2019","volume":"47","author":"Madeira","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jbc.2022.101763_bib40","first-page":"191","article-title":"Differential scanning calorimetry","volume":"40","author":"Freire","year":"1995","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jbc.2022.101763_bib41","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.ymeth.2011.06.010","article-title":"Automated data collection for macromolecular crystallography","volume":"55","author":"Winter","year":"2011","journal-title":"Methods"},{"key":"10.1016\/j.jbc.2022.101763_bib42","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"Xds: Integration, scaling, space-group assigment and post refinement","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2022.101763_bib43","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.jbc.2022.101763_bib44","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2022.101763_bib45","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1107\/S0907444994003112","article-title":"The CCP4 suite: Programs for protein crystallography","volume":"50","author":"Collaborative Computational Project","year":"1994","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2022.101763_bib46","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1038\/nsb1203-980","article-title":"Announcing the worldwide protein Data Bank","volume":"10","author":"Berman","year":"2003","journal-title":"Nat. Struct. Biol."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925822002034?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925822002034?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T03:15:23Z","timestamp":1720062923000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925822002034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["S0021925822002034"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2022.101763","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of high-affinity nanobodies specific for NaV1.4 and NaV1.5 voltage-gated sodium channel isoforms","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2022.101763","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"101763"}}