{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T04:01:41Z","timestamp":1793246501610,"version":"4.5.0"},"reference-count":38,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T00:00:00Z","timestamp":1668124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012515","name":"Cyrus Tang Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012515","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903620"],"award-info":[{"award-number":["81903620"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81922067"],"award-info":[{"award-number":["81922067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073856"],"award-info":[{"award-number":["82073856"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82202956"],"award-info":[{"award-number":["82202956"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20190306"],"award-info":[{"award-number":["BK20190306"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["020814380160"],"award-info":[{"award-number":["020814380160"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1016\/j.jbc.2022.102696","type":"journal-article","created":{"date-parts":[[2022,11,13]],"date-time":"2022-11-13T13:28:57Z","timestamp":1668346137000},"page":"102696","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"title":["Spirodalesol analog 8A inhibits NLRP3 inflammasome activation and attenuates inflammatory disease by directly targeting adaptor protein ASC"],"prefix":"10.1016","volume":"298","author":[{"given":"Wen","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihao","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyang","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiancong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renxiang","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2022.102696_bib1","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.cell.2016.03.046","article-title":"Inflammasome complexes: emerging mechanisms and effector functions","volume":"165","author":"Rathinam","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2022.102696_bib2","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/s41392-021-00650-z","article-title":"Inflammasomes as therapeutic targets in human diseases","volume":"6","author":"Li","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"key":"10.1016\/j.jbc.2022.102696_bib3","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1083\/jcb.201602089","article-title":"The cell biology of inflammasomes: mechanisms of inflammasome activation and regulation","volume":"213","author":"Sharma","year":"2016","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.jbc.2022.102696_bib4","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1016\/j.tcb.2021.06.010","article-title":"Cell biology of inflammasome activation","volume":"31","author":"Pandey","year":"2021","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.jbc.2022.102696_bib5","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1007\/s00018-016-2396-6","article-title":"Assembly and regulation of ASC specks","volume":"74","author":"Hoss","year":"2017","journal-title":"Cell Mol. Life Sci."},{"key":"10.1016\/j.jbc.2022.102696_bib6","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.it.2019.09.005","article-title":"Emerging activators and regulators of inflammasomes and pyroptosis","volume":"40","author":"Xue","year":"2019","journal-title":"Trends Immunol."},{"key":"10.1016\/j.jbc.2022.102696_bib7","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.coi.2017.10.011","article-title":"Recent advances in inflammasome biology","volume":"50","author":"Place","year":"2017","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.jbc.2022.102696_bib8","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/s41586-018-0761-3","article-title":"PtdIns4P on dispersed trans-Golgi network mediates NLRP3 inflammasome activation","volume":"564","author":"Chen","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2022.102696_bib9","doi-asserted-by":"crossref","first-page":"6299","DOI":"10.1016\/j.cell.2021.11.011","article-title":"NLRP3 cages revealed by full-length mouse NLRP3 structure control pathway activation","volume":"184","author":"Andreeva","year":"2021","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2022.102696_bib10","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/s41586-019-1295-z","article-title":"Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome","volume":"570","author":"Sharif","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2022.102696_bib11","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1038\/s41419-019-1413-8","article-title":"Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors","volume":"10","author":"Yang","year":"2019","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.jbc.2022.102696_bib12","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/s41577-019-0165-0","article-title":"The NLRP3 inflammasome: molecular activation and regulation to therapeutics","volume":"19","author":"Swanson","year":"2019","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jbc.2022.102696_bib13","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/s41577-019-0215-7","article-title":"DAMP-sensing receptors in sterile inflammation and inflammatory diseases","volume":"20","author":"Gong","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jbc.2022.102696_bib14","doi-asserted-by":"crossref","first-page":"2880","DOI":"10.1016\/j.apsb.2021.03.011","article-title":"Lonicerin targets EZH2 to alleviate ulcerative colitis by autophagy-mediated NLRP3 inflammasome inactivation","volume":"11","author":"Lv","year":"2021","journal-title":"Acta Pharm. Sin. B"},{"key":"10.1016\/j.jbc.2022.102696_bib15","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/nrd.2018.97","article-title":"Targeting the NLRP3 inflammasome in inflammatory diseases","volume":"17","author":"Mangan","year":"2018","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jbc.2022.102696_bib16","doi-asserted-by":"crossref","first-page":"6496","DOI":"10.1021\/acs.orglett.6b03435","article-title":"Spirodalesol, an NLRP3 inflammasome activation inhibitor","volume":"18","author":"Zhang","year":"2016","journal-title":"Org. Lett."},{"key":"10.1016\/j.jbc.2022.102696_bib17","doi-asserted-by":"crossref","first-page":"972","DOI":"10.4161\/auto.28374","article-title":"Small molecule-driven mitophagy-mediated NLRP3 inflammasome inhibition is responsible for the prevention of colitis-associated cancer","volume":"10","author":"Guo","year":"2014","journal-title":"Autophagy"},{"key":"10.1016\/j.jbc.2022.102696_bib18","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/ni0311-199","article-title":"Mitochondrial control of the NLRP3 inflammasome","volume":"12","author":"Kepp","year":"2011","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.jbc.2022.102696_bib19","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/nature09663","article-title":"A\u00a0role for mitochondria in NLRP3 inflammasome activation","volume":"469","author":"Zhou","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2022.102696_bib20","doi-asserted-by":"crossref","first-page":"13280","DOI":"10.1038\/ncomms13280","article-title":"PKM2-dependent glycolysis promotes NLRP3 and AIM2 inflammasome activation","volume":"7","author":"Xie","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2022.102696_bib21","doi-asserted-by":"crossref","first-page":"2403","DOI":"10.4049\/jimmunol.1700796","article-title":"Targeting peroxiredoxin 1 by a curcumin analogue, AI-44, inhibits NLRP3 inflammasome activation and attenuates lipopolysaccharide-induced sepsis in mice","volume":"201","author":"Liu","year":"2018","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.jbc.2022.102696_bib22","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1016\/j.immuni.2013.05.015","article-title":"Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation","volume":"38","author":"Yan","year":"2013","journal-title":"Immunity"},{"key":"10.1016\/j.jbc.2022.102696_bib23","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/nm.3804","article-title":"The ketone metabolite beta-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease","volume":"21","author":"Youm","year":"2015","journal-title":"Nat. Med."},{"key":"10.1016\/j.jbc.2022.102696_bib24","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nm.3806","article-title":"A\u00a0small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases","volume":"21","author":"Coll","year":"2015","journal-title":"Nat. Med."},{"key":"10.1016\/j.jbc.2022.102696_bib25","doi-asserted-by":"crossref","first-page":"2550","DOI":"10.1038\/s41467-018-04947-6","article-title":"Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity","volume":"9","author":"He","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2022.102696_bib26","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.201708689","article-title":"Tranilast directly targets NLRP3 to treat inflammasome-driven diseases","volume":"10","author":"Huang","year":"2018","journal-title":"EMBO Mol. Med."},{"key":"10.1016\/j.jbc.2022.102696_bib27","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/s41589-019-0277-7","article-title":"MCC950 directly targets the NLRP3 ATP-hydrolysis motif for inflammasome inhibition","volume":"15","author":"Coll","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.jbc.2022.102696_bib28","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1038\/s41589-019-0278-6","article-title":"MCC950 closes the active conformation of NLRP3 to an inactive state","volume":"15","author":"Tapia-Abellan","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.jbc.2022.102696_bib29","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1038\/s41423-021-00683-y","article-title":"RRx-001 ameliorates inflammatory diseases by acting as a potent covalent NLRP3 inhibitor","volume":"18","author":"Chen","year":"2021","journal-title":"Cell Mol. Immunol."},{"key":"10.1016\/j.jbc.2022.102696_bib30","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1186\/s12974-020-01826-0","article-title":"IC100: a novel anti-ASC monoclonal antibody improves functional outcomes in an animal model of multiple sclerosis","volume":"17","author":"Desu","year":"2020","journal-title":"J.\u00a0Neuroinflammation"},{"key":"10.1016\/j.jbc.2022.102696_bib31","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/imr.12296","article-title":"Regulation of inflammasome activation","volume":"265","author":"Man","year":"2015","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.jbc.2022.102696_bib32","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1111\/j.1365-2443.2004.00789.x","article-title":"ASC is essential for LPS-induced activation of procaspase-1 independently of TLR-associated signal adaptor molecules","volume":"9","author":"Yamamoto","year":"2004","journal-title":"Genes Cells"},{"key":"10.1016\/j.jbc.2022.102696_bib33","doi-asserted-by":"crossref","first-page":"3173","DOI":"10.4049\/jimmunol.0802367","article-title":"Activation of inflammasomes requires intracellular redistribution of the apoptotic speck-like protein containing a caspase recruitment domain","volume":"182","author":"Bryan","year":"2009","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.jbc.2022.102696_bib34","doi-asserted-by":"crossref","first-page":"2168","DOI":"10.1038\/s41467-017-02351-0","article-title":"Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis","volume":"8","author":"Guo","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2022.102696_bib35","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.apsb.2018.08.009","article-title":"SHP2 inhibition triggers anti-tumor immunity and synergizes with PD-1 blockade","volume":"9","author":"Zhao","year":"2019","journal-title":"Acta Pharm. Sin. B"},{"key":"10.1016\/j.jbc.2022.102696_bib36","doi-asserted-by":"crossref","first-page":"115495","DOI":"10.1016\/j.taap.2021.115495","article-title":"NLRP3 protects mice from radiation-induced colon and skin damage via attenuating cGAS-STING signaling","volume":"418","author":"Wu","year":"2021","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.jbc.2022.102696_bib37","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1233606","article-title":"Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay","volume":"341","author":"Martinez Molina","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.jbc.2022.102696_bib38","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.abe2933","article-title":"Pharmacological targeting of NLRP3 deubiquitination for treatment of NLRP3-associated inflammatory diseases","volume":"6","author":"Ren","year":"2021","journal-title":"Sci. Immunol."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925822011395?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925822011395?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:33:03Z","timestamp":1758241983000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925822011395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":38,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["S0021925822011395"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2022.102696","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Spirodalesol analog 8A inhibits NLRP3 inflammasome activation and attenuates inflammatory disease by directly targeting adaptor protein ASC","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2022.102696","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"102696"}}