{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T18:30:40Z","timestamp":1775759440958,"version":"3.50.1"},"reference-count":49,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T00:00:00Z","timestamp":1683936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI173995"],"award-info":[{"award-number":["R21AI173995"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"National Institute of Allergy and Infectious Diseases Division of Microbiology and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI125657"],"award-info":[{"award-number":["R01AI125657"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.jbc.2023.104824","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T16:49:37Z","timestamp":1684169377000},"page":"104824","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"title":["Plasmodium parasitophorous vacuole membrane protein Pfs16 promotes malaria transmission by silencing mosquito immunity"],"prefix":"10.1016","volume":"299","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7465-4512","authenticated-orcid":false,"given":"Julian","family":"Ramelow","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yacob","family":"Keleta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guodong","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2023.104824_bib1","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.chom.2017.07.003","article-title":"The molecular basis of erythrocyte invasion by malaria parasites","volume":"22","author":"Cowman","year":"2017","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.jbc.2023.104824_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.1000406","article-title":"A\u00a0research agenda to underpin malaria eradication","volume":"8","author":"Alonso","year":"2011","journal-title":"PLoS Med."},{"key":"10.1016\/j.jbc.2023.104824_bib3","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.pt.2019.11.006","article-title":"Hardly vacuous: the parasitophorous vacuolar membrane of malaria parasites","volume":"36","author":"Goldberg","year":"2020","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.jbc.2023.104824_bib4","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1038\/73062","article-title":"Malaria transmission-blocking vaccines--how can their development be supported?","volume":"6","author":"Carter","year":"2000","journal-title":"Nat. Med."},{"key":"10.1016\/j.jbc.2023.104824_bib5","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1186\/s12936-021-03723-0","article-title":"Are malaria transmission-blocking vaccines acceptable to high burden communities? Results from a mixed methods study in Bo, Sierra Leone","volume":"20","author":"McCoy","year":"2021","journal-title":"Malar. J."},{"key":"10.1016\/j.jbc.2023.104824_bib6","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1016\/S0264-410X(00)00521-1","article-title":"Transmission blocking malaria vaccines","volume":"19","author":"Carter","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.jbc.2023.104824_bib7","doi-asserted-by":"crossref","first-page":"3543","DOI":"10.1038\/s41598-018-21801-3","article-title":"Sequential membrane rupture and vesiculation during Plasmodium berghei gametocyte egress from the red blood cell","volume":"8","author":"Andreadaki","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbc.2023.104824_bib8","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1038\/s41541-021-00401-9","article-title":"Molecular interactions between parasite and mosquito during midgut invasion as targets to block malaria transmission","volume":"6","author":"Keleta","year":"2021","journal-title":"NPJ Vaccines"},{"key":"10.1016\/j.jbc.2023.104824_bib9","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2021.654216","article-title":"Studies of the parasite-midgut interaction reveal Plasmodium proteins important for malaria transmission to mosquitoes","volume":"11","author":"Niu","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.jbc.2023.104824_bib10","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-71186-5","article-title":"Analysis of blood-induced Anopheles gambiae midgut proteins and sexual stage Plasmodium falciparum interaction reveals mosquito genes important for malaria transmission","volume":"10","author":"Cui","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbc.2023.104824_bib11","doi-asserted-by":"crossref","DOI":"10.4269\/ajtmh.21-1325","article-title":"Pfs47 as a malaria transmission-blocking vaccine target","author":"Molina-Cruz","year":"2022","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.jbc.2023.104824_bib12","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1073\/pnas.1917042117","article-title":"Plasmodium falciparum evades immunity of anopheline mosquitoes by interacting with a Pfs47 midgut receptor","volume":"117","author":"Molina-Cruz","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104824_bib13","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0166-6851(97)00123-0","article-title":"Developmentally regulated expression of pfs16, a marker for sexual differentiation of the human malaria parasite Plasmodium falciparum","volume":"89","author":"Dechering","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.jbc.2023.104824_bib14","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1017\/S0031182000068219","article-title":"Subcellular localization of Pfs16, a Plasmodium falciparum gametocyte antigen","volume":"108","author":"Baker","year":"1994","journal-title":"Parasitology"},{"key":"10.1016\/j.jbc.2023.104824_bib15","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.molbiopara.2003.10.014","article-title":"Functional analysis of Plasmodium falciparum parasitophorous vacuole membrane protein (Pfs16) during gametocytogenesis and gametogenesis by targeted gene disruption","volume":"133","author":"Kongkasuriyachai","year":"2004","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.jbc.2023.104824_bib16","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.immuni.2006.08.019","article-title":"Boosting NF-kappaB-dependent basal immunity of Anopheles gambiae aborts development of Plasmodium berghei","volume":"25","author":"Frolet","year":"2006","journal-title":"Immunity"},{"key":"10.1016\/j.jbc.2023.104824_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000335","article-title":"Caspar controls resistance to Plasmodium falciparum in diverse anopheline species","volume":"5","author":"Garver","year":"2009","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jbc.2023.104824_bib18","doi-asserted-by":"crossref","first-page":"11420","DOI":"10.1073\/pnas.0504950102","article-title":"Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae","volume":"102","author":"Meister","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104824_bib19","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1002\/dvdy.20283","article-title":"Dissecting JNK signaling, one KKKinase at a time","volume":"232","author":"Stronach","year":"2005","journal-title":"Dev. Dyn."},{"key":"10.1016\/j.jbc.2023.104824_bib20","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1186\/1471-2164-12-574","article-title":"The mitogen-activated protein kinome from Anopheles gambiae: identification, phylogeny and functional characterization of the ERK, JNK and p38 MAP kinases","volume":"12","author":"Horton","year":"2011","journal-title":"BMC Genomics"},{"key":"10.1016\/j.jbc.2023.104824_bib21","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S1534-5807(02)00325-8","article-title":"Sequential activation of signaling pathways during innate immune responses in Drosophila","volume":"3","author":"Boutros","year":"2002","journal-title":"Dev. Cell"},{"key":"10.1016\/j.jbc.2023.104824_bib22","doi-asserted-by":"crossref","first-page":"682","DOI":"10.18632\/genesandcancer.155","article-title":"JNK-signaling: a multiplexing hub in programmed cell death","volume":"8","author":"Dhanasekaran","year":"2017","journal-title":"Genes Cancer"},{"key":"10.1016\/j.jbc.2023.104824_bib23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003622","article-title":"The JNK pathway is a key mediator of Anopheles gambiae antiplasmodial immunity","volume":"9","author":"Garver","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jbc.2023.104824_bib24","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1073\/pnas.1423586112","article-title":"Plasmodium falciparum evades mosquito immunity by disrupting JNK-mediated apoptosis of invaded midgut cells","volume":"112","author":"Ramphul","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104824_bib25","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1101\/gad.1168104","article-title":"Targeting of TAK1 by the NF-kappa B protein Relish regulates the JNK-mediated immune response in Drosophila","volume":"18","author":"Park","year":"2004","journal-title":"Genes Dev."},{"key":"10.1016\/j.jbc.2023.104824_bib26","doi-asserted-by":"crossref","first-page":"48928","DOI":"10.1074\/jbc.M304802200","article-title":"Immune activation of NF-kappaB and JNK requires Drosophila TAK1","volume":"278","author":"Silverman","year":"2003","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2023.104824_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002737","article-title":"Anopheles Imd pathway factors and effectors in infection intensity-dependent anti-Plasmodium action","volume":"8","author":"Garver","year":"2012","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jbc.2023.104824_bib28","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1038\/s41587-021-01156-3","article-title":"SignalP 6.0 predicts all five types of signal peptides using protein language models","volume":"40","author":"Teufel","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jbc.2023.104824_bib29","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.jbc.2023.104824_bib30","doi-asserted-by":"crossref","first-page":"5553","DOI":"10.3390\/ijms22115553","article-title":"Deep learning-based advances in protein structure prediction","volume":"22","author":"Pakhrin","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbc.2023.104824_bib31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1009394","article-title":"Transport mechanisms at the malaria parasite-host cell interface","volume":"17","author":"Beck","year":"2021","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jbc.2023.104824_bib32","doi-asserted-by":"crossref","first-page":"16490","DOI":"10.1074\/jbc.M114.623165","article-title":"Anopheles midgut FREP1 mediates Plasmodium invasion","volume":"290","author":"Zhang","year":"2015","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2023.104824_bib33","article-title":"Targeting Plasmodium \u03b1-tubulin-1 to block malaria transmission to mosquitoes","volume":"13","author":"Zhang","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.jbc.2023.104824_bib34","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-53208-z","article-title":"Engineering a virus-like particle as an antigenic platform for a Pfs47-targeted malaria transmission-blocking vaccine","volume":"9","author":"Yenkoidiok-Douti","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbc.2023.104824_bib35","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1186\/s12936-020-03368-5","article-title":"Particle-based, Pfs230 and Pfs25 immunization is effective, but not improved by duplexing at fixed total antigen dose","volume":"19","author":"Huang","year":"2020","journal-title":"Malar. J."},{"key":"10.1016\/j.jbc.2023.104824_bib36","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1038\/nsmb.3048","article-title":"The Anopheles-midgut APN1 structure reveals a new malaria transmission-blocking vaccine epitope","volume":"22","author":"Atkinson","year":"2015","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.jbc.2023.104824_bib37","doi-asserted-by":"crossref","first-page":"13461","DOI":"10.1073\/pnas.0702239104","article-title":"Disruption of Plasmodium falciparum development by antibodies against a conserved mosquito midgut antigen","volume":"104","author":"Dinglasan","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104824_bib38","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.694759","article-title":"Safety and immunogenicity of ChAd63\/MVA Pfs25-IMX313 in a phase I first-in-human trial","volume":"12","author":"de Graaf","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jbc.2023.104824_bib39","doi-asserted-by":"crossref","first-page":"20675","DOI":"10.1073\/pnas.1321024110","article-title":"Genome-block expression-assisted association studies discover malaria resistance genes in Anopheles gambiae","volume":"110","author":"Li","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104824_bib40","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.10392","article-title":"A\u00a0diverse global fungal library for drug discovery","volume":"8","author":"Niu","year":"2020","journal-title":"PeerJ."},{"key":"10.1016\/j.jbc.2023.104824_bib41","doi-asserted-by":"crossref","first-page":"3018","DOI":"10.3390\/molecules25133018","article-title":"Fungal metabolite asperaculane B inhibits malaria infection and transmission","volume":"25","author":"Niu","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.jbc.2023.104824_bib42","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1186\/s13071-021-04677-7","article-title":"A\u00a0novel fungal metabolite inhibits Plasmodium falciparum transmission and infection","volume":"14","author":"Niu","year":"2021","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.jbc.2023.104824_bib43","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1128\/EC.00008-11","article-title":"Protein targeting to the parasitophorous vacuole membrane of Plasmodium falciparum","volume":"10","author":"Eksi","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.jbc.2023.104824_bib44","doi-asserted-by":"crossref","first-page":"3439","DOI":"10.1073\/pnas.1619441114","article-title":"Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress","volume":"114","author":"Hale","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104824_bib45","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1462-5822.2008.01242.x","article-title":"Irreversible effect of cysteine protease inhibitors on the release of malaria parasites from infected erythrocytes","volume":"11","author":"Glushakova","year":"2009","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.jbc.2023.104824_bib46","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.pt.2015.06.009","article-title":"Exosomes and other extracellular vesicles: the new communicators in parasite infections","volume":"31","author":"Coakley","year":"2015","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.jbc.2023.104824_bib47","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1038\/s41579-019-0321-3","article-title":"The parasitophorous vacuole of the blood-stage malaria parasite","volume":"18","author":"Matz","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jbc.2023.104824_bib48","series-title":"Specificity of the Innate Immune System: A Closer Look at the Mosquito Pattern-Recognition Receptor Repertoire","author":"Das","year":"2009"},{"key":"10.1016\/j.jbc.2023.104824_bib49","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0057909","article-title":"Qualification of standard membrane-feeding assay with Plasmodium falciparum malaria and potential improvements for future assays","volume":"8","author":"Miura","year":"2013","journal-title":"PLoS One"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925823018525?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925823018525?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:47:10Z","timestamp":1758242830000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925823018525"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["S0021925823018525"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2023.104824","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Plasmodium parasitophorous vacuole membrane protein Pfs16 promotes malaria transmission by silencing mosquito immunity","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2023.104824","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"104824"}}