{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:31:12Z","timestamp":1792798272230,"version":"4.5.0"},"reference-count":48,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2023,7]]},"DOI":"10.1016\/j.jbc.2023.104860","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T20:35:22Z","timestamp":1684960522000},"page":"104860","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Structure of complex III with bound antimalarial agent CK-2-68 provides insights into selective inhibition of Plasmodium cytochrome bc1 complexes"],"prefix":"10.1016","volume":"299","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8680-6851","authenticated-orcid":false,"given":"Lothar","family":"Esser","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison","family":"Zeher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3336-9635","authenticated-orcid":false,"given":"Rick","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-An","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4352-0562","authenticated-orcid":false,"given":"Kristin D.","family":"Lane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Wellems","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2023.104860_bib1","series-title":"Global Malaria Programme","author":"Organization","year":"2021"},{"key":"10.1016\/j.jbc.2023.104860_bib2","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1046\/j.1365-2958.1999.01515.x","article-title":"Resistance mutations reveal the atovaquone-binding domain of cytochrome b in malaria parasites","volume":"33","author":"Srivastava","year":"1999","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jbc.2023.104860_bib3","doi-asserted-by":"crossref","first-page":"2817","DOI":"10.1074\/jbc.M309984200","article-title":"Molecular basis for atovaquone resistance in Pneumocystis jirovecii modeled in the cytochrome bc(1) complex of Saccharomyces cerevisiae","volume":"279","author":"Kessl","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2023.104860_bib4","doi-asserted-by":"crossref","first-page":"3820","DOI":"10.1038\/s41467-021-23919-x","article-title":"Composition and stage dynamics of mitochondrial complexes in Plasmodium falciparum","volume":"12","author":"Evers","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2023.104860_bib5","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1128\/AAC.50.5.1841-1851.2006","article-title":"Functional characterization and target validation of alternative complex I of Plasmodium falciparum mitochondria","volume":"50","author":"Biagini","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jbc.2023.104860_bib6","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1021\/jm201184h","article-title":"Identification, design and biological evaluation of heterocyclic quinolones targeting Plasmodium falciparum type II NADH:quinone oxidoreductase (PfNDH2)","volume":"55","author":"Leung","year":"2012","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2023.104860_bib7","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1021\/jm201179h","article-title":"Identification, design and biological evaluation of bisaryl quinolones targeting Plasmodium falciparum type II NADH:quinone oxidoreductase (PfNDH2)","volume":"55","author":"Pidathala","year":"2012","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2023.104860_bib8","doi-asserted-by":"crossref","first-page":"1994","DOI":"10.1021\/acs.jmedchem.6b01733","article-title":"Target elucidation by cocrystal structures of NADH-ubiquinone oxidoreductase of Plasmodium falciparum (PfNDH2) with small molecule to eliminate drug-resistant malaria","volume":"60","author":"Yang","year":"2017","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2023.104860_bib9","doi-asserted-by":"crossref","first-page":"6285","DOI":"10.1073\/pnas.1804492115","article-title":"Selection of Plasmodium falciparum cytochrome B mutants by putative PfNDH2 inhibitors","volume":"115","author":"Lane","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104860_bib10","doi-asserted-by":"crossref","first-page":"32661","DOI":"10.1074\/jbc.M111.269399","article-title":"Arrested oocyst maturation in Plasmodium parasites lacking type II NADH:ubiquinone dehydrogenase","volume":"286","author":"Boysen","year":"2011","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2023.104860_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0214023","article-title":"Mitochondrial type II NADH dehydrogenase of Plasmodium falciparum (PfNDH2) is dispensable in the asexual blood stages","volume":"14","author":"Ke","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.jbc.2023.104860_bib12","doi-asserted-by":"crossref","first-page":"8298","DOI":"10.1073\/pnas.1205651109","article-title":"Generation of quinolone antimalarials targeting the Plasmodium falciparum mitochondrial respiratory chain for the treatment and prophylaxis of malaria","volume":"109","author":"Biagini","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104860_bib13","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1124\/mol.108.045120","article-title":"Acridinediones: selective and potent inhibitors of the malaria parasite mitochondrial bc1 complex","volume":"73","author":"Biagini","year":"2008","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.jbc.2023.104860_bib14","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1073\/pnas.1416611112","article-title":"Antimalarial 4(1H)-pyridones bind to the Qi site of cytochrome bc1","volume":"112","author":"Capper","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104860_bib15","doi-asserted-by":"crossref","first-page":"4029","DOI":"10.1038\/ncomms5029","article-title":"Structural analysis of atovaquone-inhibited cytochrome bc1 complex reveals the molecular basis of antimalarial drug action","volume":"5","author":"Birth","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2023.104860_bib16","doi-asserted-by":"crossref","first-page":"25019","DOI":"10.1074\/jbc.M116.744391","article-title":"Hydrogen bonding to the substrate is not required for rieske iron-sulfur protein docking to the quinol oxidation site of complex III","volume":"291","author":"Esser","year":"2016","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2023.104860_bib17","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/0005-2728(81)90238-3","article-title":"Myxothiazol, a new inhibitor of the cytochrome b-c1 segment of th respiratory chain","volume":"638","author":"Thierbach","year":"1981","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jbc.2023.104860_bib18","doi-asserted-by":"crossref","first-page":"177ra37","DOI":"10.1126\/scitranslmed.3005029","article-title":"Quinolone-3-diarylethers: a new class of antimalarial drug","volume":"5","author":"Nilsen","year":"2013","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.jbc.2023.104860_bib19","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/j.bbabio.2006.05.031","article-title":"Generation, characterization and crystallization of a highly active and stable cytochrome bc1 complex mutant from Rhodobacter sphaeroides","volume":"1757","author":"Elberry","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jbc.2023.104860_bib20","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.jmb.2004.05.065","article-title":"Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex","volume":"341","author":"Esser","year":"2004","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.jbc.2023.104860_bib21","doi-asserted-by":"crossref","first-page":"13045","DOI":"10.1073\/pnas.0601149103","article-title":"Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex","volume":"103","author":"Esser","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2023.104860_bib22","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1016\/S0006-3495(99)77022-0","article-title":"Steered molecular dynamics simulation of teh rieske subunit motion in the cytochrome bc1 complex","volume":"77","author":"Izrailev","year":"1999","journal-title":"Biophys. J."},{"key":"10.1016\/j.jbc.2023.104860_bib23","series-title":"Crystal Structure of Bovine Cytochrome Bc1 in Complex with Quinolone Inhibitor CK-2-68","author":"Amporndanai","year":"2021"},{"key":"10.1016\/j.jbc.2023.104860_bib24","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1126\/science.277.5322.60","article-title":"Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria","volume":"277","author":"Xia","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.jbc.2023.104860_bib25","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2023.104860_bib26","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/0169-4758(93)90040-M","article-title":"Atovaquone - a novel broad-spectrum anti-infective drug","volume":"9","author":"Hudson","year":"1993","journal-title":"Parasitol. Today"},{"key":"10.1016\/j.jbc.2023.104860_bib27","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1042\/bst0270577","article-title":"Famoxadone and oxazolidinones: potent inhibitors of cytochrome bc1","volume":"27","author":"Jordan","year":"1999","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.jbc.2023.104860_bib28","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1002\/(SICI)1096-9063(199902)55:2<105::AID-PS879>3.0.CO;2-D","article-title":"Mode of action of famoxadone","volume":"55","author":"Jordan","year":"1999","journal-title":"Pestic. Sci."},{"key":"10.1016\/j.jbc.2023.104860_bib29","doi-asserted-by":"crossref","first-page":"11692","DOI":"10.1021\/bi026252p","article-title":"The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition","volume":"41","author":"Gao","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbc.2023.104860_bib30","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1002\/ps.565","article-title":"Mechanisms influencing the evolution of resistance to Qo inhibitor fungicides","volume":"58","author":"Gisi","year":"2002","journal-title":"Pest Manag. Sci."},{"key":"10.1016\/j.jbc.2023.104860_bib31","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1128\/AAC.44.8.2100-2108.2000","article-title":"Mutations in Plasmodium falciparum cytochrome b that are associated with atovaquone resistance are located at a putative drug-binding site","volume":"44","author":"Korsinczky","year":"2000","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jbc.2023.104860_bib32","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1039\/jr9470001034","article-title":"Tetrahydroacridones and related compounds as antimalarials","author":"Stephen","year":"1947","journal-title":"J.\u00a0Chem. Soc."},{"key":"10.1016\/j.jbc.2023.104860_bib33","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1107\/S2052252518001616","article-title":"X-ray and cryo-EM structures of inhibitor-bound cytochrome bc1 complexes for structure-based drug discovery","volume":"5","author":"Amporndanai","year":"2018","journal-title":"IUCrJ"},{"key":"10.1016\/j.jbc.2023.104860_bib34","first-page":"1109","article-title":"Targeting the ubiquinol-reduction (Qi) site of the mitochondrial cytochrome bc1 complex for the development of next generation quinolone antimalarials","volume":"11","author":"Amporndanai","year":"2022","journal-title":"Biology (Basel)"},{"key":"10.1016\/j.jbc.2023.104860_bib35","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1021\/acsmedchemlett.8b00371","article-title":"Potent antimalarial 2-pyrazolyl quinolone bc 1 (Qi) inhibitors with improved drug-like properties","volume":"9","author":"David Hong","year":"2018","journal-title":"ACS Med. Chem. Lett."},{"key":"10.1016\/j.jbc.2023.104860_bib36","doi-asserted-by":"crossref","first-page":"203","DOI":"10.3389\/fcimb.2020.00203","article-title":"Potent tetrahydroquinolone eliminates apicomplexan parasites","volume":"10","author":"McPhillie","year":"2020","journal-title":"Front. Cell Infect. Microbiol"},{"key":"10.1016\/j.jbc.2023.104860_bib37","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1128\/AAC.04149-14","article-title":"Subtle changes in endochin-like quinolone structure alter the site of inhibition within the cytochrome bc1 complex of Plasmodium falciparum","volume":"59","author":"Stickles","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jbc.2023.104860_bib38","doi-asserted-by":"crossref","first-page":"4096","DOI":"10.1021\/bi00260a028","article-title":"Syntheses of biologically active ubiquinone derivatives","volume":"21","author":"Yu","year":"1982","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbc.2023.104860_bib39","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S0076-6879(08)04425-X","article-title":"Chapter 25 Analysis of electron transfer and superoxide generation in the cytochrome bc1 complex","volume":"456","author":"Yu","year":"2009","journal-title":"Met. Enzymol."},{"key":"10.1016\/j.jbc.2023.104860_bib40","article-title":"Studies on inhibition of respiratory cytochrome bc1 complex by the fungicide pyrimorph suggest a novel inhibitory mechanism","volume":"9","author":"Xiao","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jbc.2023.104860_bib41","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.35383","article-title":"cisTEM, user-friendly software for single-particle image processing","volume":"7","author":"Grant","year":"2018","journal-title":"Elife"},{"key":"10.1016\/j.jbc.2023.104860_bib42","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"Phenix: a comprehensive python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. Sect. D"},{"key":"10.1016\/j.jbc.2023.104860_bib43","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1107\/S0907444902016657","article-title":"Phenix: building new software for automated crystallographic structure determination","volume":"58","author":"Adams","year":"2002","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2023.104860_bib44","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2023.104860_bib45","doi-asserted-by":"crossref","first-page":"D396","DOI":"10.1093\/nar\/gkv1126","article-title":"EMDataBank unified data resource for 3DEM","volume":"44","author":"Lawson","year":"2016","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.jbc.2023.104860_bib46","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","article-title":"The protein Data Bank","volume":"28","author":"Berman","year":"2000","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.jbc.2023.104860_bib47","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1107\/S0021889891004399","article-title":"Molscript - a program to produce both detailed and schematic plots of protein structures","volume":"24","author":"Kraulis","year":"1991","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.jbc.2023.104860_bib48","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1107\/S0907444998017363","article-title":"Further additions to MolScript version 1.4, including reading and contouring of electron-density maps","volume":"55","author":"Esnouf","year":"1999","journal-title":"Acta Crystallogr. D Biol. Crystallogr."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925823018884?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925823018884?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:34:46Z","timestamp":1758242086000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925823018884"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7]]},"references-count":48,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["S0021925823018884"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2023.104860","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2023,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structure of complex III with bound antimalarial agent CK-2-68 provides insights into selective inhibition of Plasmodium cytochrome bc1 complexes","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2023.104860","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"104860"}}