{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:37:14Z","timestamp":1782779834662,"version":"3.54.5"},"reference-count":39,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T00:00:00Z","timestamp":1687392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009666","name":"Salt Science Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009666","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101102"],"award-info":[{"award-number":["82101102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515012506"],"award-info":[{"award-number":["2022A1515012506"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2701800"],"award-info":[{"award-number":["2021YFC2701800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2023,8]]},"DOI":"10.1016\/j.jbc.2023.104953","type":"journal-article","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T10:42:15Z","timestamp":1687603335000},"page":"104953","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Cataract-causing Y204X mutation of crystallin protein CRY\u03b2B1 promotes its C-terminal degradation and higher-order oligomerization"],"prefix":"10.1016","volume":"299","author":[{"given":"Xuping","family":"Jing","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingwei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyu","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bu-Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ping","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dao-Man","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2023.104953_bib1","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.1110\/ps.03265903","article-title":"Crystal structure of truncated human betaB1-crystallin","volume":"12","author":"Van Montfort","year":"2003","journal-title":"Protein Sci."},{"key":"10.1016\/j.jbc.2023.104953_bib2","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.ijbiomac.2017.12.013","article-title":"Increasing susceptibility to oxidative stress by cataract-causing crystallin mutations","volume":"108","author":"Zhao","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.jbc.2023.104953_bib3","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1098\/rstb.2010.0227","article-title":"Clinical and experimental advances in congenital and paediatric cataracts","volume":"366","author":"Churchill","year":"2011","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"10.1016\/j.jbc.2023.104953_bib4","first-page":"624","article-title":"A\u00a0novel truncation mutation in CRYBB1 associated with autosomal dominant congenital cataract with nystagmus","volume":"23","author":"Rao","year":"2017","journal-title":"Mol. Vis."},{"key":"10.1016\/j.jbc.2023.104953_bib5","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.str.2017.02.001","article-title":"Human betaB2-Crystallin forms a face-en-face dimer in solution: an integrated NMR and SAXS study","volume":"25","author":"Xi","year":"2017","journal-title":"Structure"},{"key":"10.1016\/j.jbc.2023.104953_bib6","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nsb0396-267","article-title":"The X-ray structures of two mutant crystallin domains shed light on the evolution of multi-domain proteins","volume":"3","author":"Norledge","year":"1996","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.jbc.2023.104953_bib7","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1006\/jmbi.2000.4197","article-title":"The N-terminal domain of betaB2-crystallin resembles the putative ancestral homodimer","volume":"304","author":"Clout","year":"2000","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.jbc.2023.104953_bib8","doi-asserted-by":"crossref","first-page":"11250","DOI":"10.1074\/jbc.272.17.11250","article-title":"Size of human lens beta-crystallin aggregates are distinguished by N-terminal truncation of betaB1","volume":"272","author":"Ajaz","year":"1997","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2023.104953_bib9","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S0167-4838(99)00123-5","article-title":"Formation of betaA3\/betaB2-crystallin mixed complexes: involvement of N- and C-terminal extensions","volume":"1432","author":"Werten","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jbc.2023.104953_bib10","doi-asserted-by":"crossref","first-page":"7179","DOI":"10.1021\/bi900313c","article-title":"Truncated human beta B1-crystallin shows altered structural properties and interaction with human beta A3-crystallin","volume":"48","author":"Srivastava","year":"2009","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbc.2023.104953_bib11","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1038\/347776a0","article-title":"X-Ray-analysis of beta-B2-crystallin and evolution of oligomeric lens proteins","volume":"347","author":"Bax","year":"1990","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2023.104953_bib12","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1107\/S0907444995014302","article-title":"An eye lens protein-water structure: 1.2 angstrom resolution structure of gamma B-crystallin at 150K","volume":"52","author":"Kumaraswamy","year":"1996","journal-title":"Acta Crystallogr. D"},{"key":"10.1016\/j.jbc.2023.104953_bib13","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1002\/pro.5560070602","article-title":"Circular permutation of beta B2-crystallin changes the hierarchy of domain assembly","volume":"7","author":"Wright","year":"1998","journal-title":"Protein Sci."},{"key":"10.1016\/j.jbc.2023.104953_bib14","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1110\/ps.062659107","article-title":"Mutation of interfaces in domain-swapped human beta B2-crystallin","volume":"16","author":"Smith","year":"2007","journal-title":"Protein Sci."},{"key":"10.1016\/j.jbc.2023.104953_bib15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0070336","article-title":"Structural integrity of the greek key motif in beta gamma-crystallins is vital for central eye lens transparency","volume":"8","author":"Vendra","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jbc.2023.104953_bib16","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1086\/344212","article-title":"A\u00a0nonsense mutation in CRYBB1 associated with autosomal dominant cataract linked to human chromosome 22q","volume":"71","author":"Mackay","year":"2002","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.jbc.2023.104953_bib17","first-page":"727","article-title":"A\u00a0novel nonsense mutation in CRYBB1 associated with autosomal dominant congenital cataract","volume":"14","author":"Yang","year":"2008","journal-title":"Mol. Vis."},{"key":"10.1016\/j.jbc.2023.104953_bib18","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.ejmg.2013.12.006","article-title":"Molecular genetics of congenital nuclear cataract","volume":"57","author":"Deng","year":"2014","journal-title":"Eur. J. Med. Genet."},{"key":"10.1016\/j.jbc.2023.104953_bib19","doi-asserted-by":"crossref","first-page":"E2050","DOI":"10.1002\/humu.21436","article-title":"A\u00a0novel mutation in CRYBB1 associated with congenital cataract-microcornea syndrome: the p.Ser129Arg mutation destabilizes the betaB1\/betaA3-crystallin heteromer but not the betaB1-crystallin homomer","volume":"32","author":"Wang","year":"2011","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.jbc.2023.104953_bib20","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1016\/j.bbadis.2014.07.022","article-title":"Cataract-causing mutation R233H affects the stabilities of betaB1- and betaA3\/betaB1-crystallins with different pH-dependence","volume":"1842","author":"Xi","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jbc.2023.104953_bib21","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1080\/02713683.2019.1675176","article-title":"A\u00a0novel mutation p.S93R in CRYBB1 associated with dominant congenital cataract and microphthalmia","volume":"45","author":"Jin","year":"2019","journal-title":"Curr. Eye Res."},{"key":"10.1016\/j.jbc.2023.104953_bib22","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1042\/BCJ20160247","article-title":"Congenital microcornea-cataract syndrome-causing mutation X253R increases betaB1-crystallin hydrophobicity to promote aggregate formation","volume":"473","author":"Leng","year":"2016","journal-title":"Biochem. J."},{"key":"10.1016\/j.jbc.2023.104953_bib23","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1007\/s13238-016-0284-3","article-title":"Cataract-causing mutation S228P promotes betaB1-crystallin aggregation and degradation by separating two interacting loops in C-terminal domain","volume":"7","author":"Qi","year":"2016","journal-title":"Protein Cell"},{"key":"10.1016\/j.jbc.2023.104953_bib24","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jbc.2023.104953_bib25","first-page":"587","article-title":"CRYBB1 mutation associated with congenital cataract and microcornea","volume":"11","author":"Willoughby","year":"2005","journal-title":"Mol. Vis."},{"key":"10.1016\/j.jbc.2023.104953_bib26","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1097\/00029330-200705010-00015","article-title":"A\u00a0missense mutation S228P in the CRYBB1 gene causes autosomal dominant congenital cataract","volume":"120","author":"Wang","year":"2007","journal-title":"Chin. Med. J. (Engl.)"},{"key":"10.1016\/j.jbc.2023.104953_bib27","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.exer.2016.06.011","article-title":"Mutations and mechanisms in congenital and age-related cataracts","volume":"156","author":"Shiels","year":"2017","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.jbc.2023.104953_bib28","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1146\/annurev-vision-091517-034346","article-title":"Biology of inherited cataracts and opportunities for treatment","volume":"5","author":"Shiels","year":"2019","journal-title":"Annu. Rev. Vis. Sci."},{"key":"10.1016\/j.jbc.2023.104953_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2021.108662","article-title":"Inherited cataracts: genetic mechanisms and pathways new and old","volume":"209","author":"Shiels","year":"2021","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.jbc.2023.104953_bib30","doi-asserted-by":"crossref","DOI":"10.1590\/1678-4685-gmb-2020-0334","article-title":"A\u00a0novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate","volume":"44","author":"Xian","year":"2021","journal-title":"Genet. Mol. Biol."},{"key":"10.1016\/j.jbc.2023.104953_bib31","doi-asserted-by":"crossref","first-page":"e174","DOI":"10.1093\/nar\/gnh172","article-title":"Site-directed, ligase-independent mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h","volume":"32","author":"Chiu","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jbc.2023.104953_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003549","article-title":"Crystal structure of the full-length Japanese encephalitis virus NS5 reveals a conserved methyltransferase-polymerase interface","volume":"9","author":"Lu","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jbc.2023.104953_bib33","first-page":"12","article-title":"The macromolecular crystallography beamline of SSRF","volume":"26","author":"Wang","year":"2015","journal-title":"Nucl. Sci. Tech."},{"key":"10.1016\/j.jbc.2023.104953_bib34","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1107\/S090744499900935X","article-title":"The finer things in X-ray diffraction data collection","volume":"55","author":"Pflugrath","year":"1999","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2023.104953_bib35","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J.\u00a0Appl. Crystallogr."},{"key":"10.1016\/j.jbc.2023.104953_bib36","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2023.104953_bib37","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1107\/S0907444902016657","article-title":"PHENIX: building new software for automated crystallographic structure determination","volume":"58","author":"Adams","year":"2002","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2023.104953_bib38","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","article-title":"Crystallography & NMR system: a new software suite for macromolecular structure determination","volume":"54","author":"Brunger","year":"1998","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2023.104953_bib39","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1093\/bioinformatics\/btl332","article-title":"THESEUS: maximum likelihood superpositioning and analysis of macromolecular structures","volume":"22","author":"Theobald","year":"2006","journal-title":"Bioinformatics"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925823019816?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925823019816?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T16:35:41Z","timestamp":1762187741000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925823019816"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":39,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["S0021925823019816"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2023.104953","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2023,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cataract-causing Y204X mutation of crystallin protein CRY\u03b2B1 promotes its C-terminal degradation and higher-order oligomerization","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2023.104953","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"104953"}}