{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T05:56:04Z","timestamp":1788587764804,"version":"build-2803163510"},"reference-count":52,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,2,24]],"date-time":"2024-02-24T00:00:00Z","timestamp":1708732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007670","name":"Dalhousie Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007670","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004336","name":"Novartis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004336","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2022-02982"],"award-info":[{"award-number":["RGPIN-2022-02982"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020170","name":"Research Nova Scotia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1016\/j.jbc.2024.107119","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T11:23:54Z","timestamp":1709119434000},"page":"107119","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Complex N-glycosylation of mGluR6 is required for trans-synaptic interaction with ELFN adhesion proteins"],"prefix":"10.1016","volume":"300","author":[{"given":"Michael L.","family":"Miller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustansir","family":"Pindwarawala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3647-1926","authenticated-orcid":false,"given":"Melina A.","family":"Agosto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2024.107119_bib1","doi-asserted-by":"crossref","first-page":"11868","DOI":"10.1016\/S0021-9258(19)50280-0","article-title":"Molecular characterization of a novel retinal metabotropic glutamate receptor mGluR6 with a high agonist selectivity for L-2-amino-4-phosphonobutyrate","volume":"268","author":"Nakajima","year":"1993","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.107119_bib2","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0092-8674(94)90151-1","article-title":"Developmentally regulated postsynaptic localization of a metabotropic glutamate receptor in rat rod bipolar cells","volume":"77","author":"Nomura","year":"1994","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2024.107119_bib3","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/0092-8674(95)90354-2","article-title":"Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene","volume":"80","author":"Masu","year":"1995","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2024.107119_bib4","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1002\/1096-9861(20000731)423:3<402::AID-CNE4>3.0.CO;2-E","article-title":"Localization of mGluR6 to dendrites of ON bipolar cells in primate retina","volume":"423","author":"Vardi","year":"2000","journal-title":"J.\u00a0Comp. Neurol."},{"key":"10.1016\/j.jbc.2024.107119_bib5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1146\/annurev-vision-102016-061338","article-title":"The transduction cascade in retinal ON-bipolar cells: signal processing and disease","volume":"3","author":"Martemyanov","year":"2017","journal-title":"Annu. Rev. Vis. Sci."},{"key":"10.1016\/j.jbc.2024.107119_bib6","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1016\/j.neuron.2015.09.002","article-title":"Mechanism for selective synaptic wiring of rod photoreceptors into the retinal circuitry and its role in vision","volume":"87","author":"Cao","year":"2015","journal-title":"Neuron"},{"key":"10.1016\/j.jbc.2024.107119_bib7","doi-asserted-by":"crossref","first-page":"23914","DOI":"10.1073\/pnas.2009940117","article-title":"Interplay between cell-adhesion molecules governs synaptic wiring of cone photoreceptors","volume":"117","author":"Cao","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.107119_bib8","doi-asserted-by":"crossref","first-page":"5022","DOI":"10.1073\/pnas.1722498115","article-title":"Synaptic adhesion protein ELFN1 is a selective allosteric modulator of group III metabotropic glutamate receptors in trans","volume":"115","author":"Dunn","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.107119_bib9","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1126\/science.1222482","article-title":"Elfn1 regulates target-specific release probability at CA1-interneuron synapses","volume":"338","author":"Sylwestrak","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.107119_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms5501","article-title":"Elfn1 recruits presynaptic mGluR7 in trans and its loss results in seizures","volume":"5","author":"Tomioka","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2024.107119_bib11","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1038\/s41380-019-0512-3","article-title":"ELFN2 is a postsynaptic cell adhesion molecule with essential roles in controlling group III mGluRs in the brain and neuropsychiatric behavior","volume":"24","author":"Dunn","year":"2019","journal-title":"Mol. Psychiatry"},{"key":"10.1016\/j.jbc.2024.107119_bib12","doi-asserted-by":"crossref","first-page":"4461","DOI":"10.1523\/JNEUROSCI.2276-18.2019","article-title":"Elfn1-induced constitutive activation of mGluR7 determines frequency-dependent recruitment of somatostatin interneurons","volume":"39","author":"Stachniak","year":"2019","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.jbc.2024.107119_bib13","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41593-020-00748-7","article-title":"Transcriptomic organization of the human brain in post-traumatic stress disorder","volume":"24","author":"Girgenti","year":"2021","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.jbc.2024.107119_bib14","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1186\/s12920-021-01066-y","article-title":"Exome sequencing identifies novel and known mutations in families with intellectual disability","volume":"14","author":"Rasheed","year":"2021","journal-title":"BMC Med. Genomics"},{"key":"10.1016\/j.jbc.2024.107119_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejmg.2021.104340","article-title":"Biallelic mutations in ELFN1 gene associated with developmental and epileptic encephalopathy and joint laxity","volume":"64","author":"Dursun","year":"2021","journal-title":"Eur. J. Med. Genet."},{"key":"10.1016\/j.jbc.2024.107119_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.neuropharm.2021.108799","article-title":"Membrane trafficking and positioning of mGluRs at presynaptic and postsynaptic sites of excitatory synapses","volume":"200","author":"Bodz\u0119ta","year":"2021","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.jbc.2024.107119_bib17","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1016\/j.neuron.2018.05.018","article-title":"Glutamatergic signaling in the central nervous system: ionotropic and metabotropic receptors in concert","volume":"98","author":"Reiner","year":"2018","journal-title":"Neuron"},{"key":"10.1016\/j.jbc.2024.107119_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2021.101418","article-title":"The mGluR6 ligand-binding domain, but not the C-terminal domain, is required for synaptic localization in retinal ON-bipolar cells","volume":"297","author":"Agosto","year":"2021","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.107119_bib19","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/j.tibs.2020.07.008","article-title":"Structural and biophysical mechanisms of class C G protein-coupled receptor function","volume":"45","author":"Ellaithy","year":"2020","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.jbc.2024.107119_bib20","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1111\/jnc.15217","article-title":"Involvement of the C-terminal domain in cell surface localization and G-protein coupling of mGluR6","volume":"158","author":"Rai","year":"2020","journal-title":"J.\u00a0Neurochem."},{"key":"10.1016\/j.jbc.2024.107119_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcn.2023.103875","article-title":"The intracellular C-terminal domain of mGluR6 contains ER retention motifs","volume":"126","author":"Shimohata","year":"2023","journal-title":"Mol. Cell. Neurosci."},{"key":"10.1016\/j.jbc.2024.107119_bib22","series-title":"Essentials of Glycobiology","year":"2022"},{"key":"10.1016\/j.jbc.2024.107119_bib23","doi-asserted-by":"crossref","first-page":"7153","DOI":"10.1167\/iovs.13-12907","article-title":"Orphan receptor GPR179 forms macromolecular complexes with components of metabotropic signaling cascade in retina ON-bipolar neurons","volume":"54","author":"Orlandi","year":"2013","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.jbc.2024.107119_bib24","doi-asserted-by":"crossref","first-page":"e001","DOI":"10.1017\/S0952523817000360","article-title":"Differential epitope masking reveals synapse-specific complexes of TRPM1","volume":"35","author":"Agosto","year":"2018","journal-title":"Vis. Neurosci."},{"key":"10.1016\/j.jbc.2024.107119_bib25","doi-asserted-by":"crossref","first-page":"4409","DOI":"10.1113\/jphysiol.2008.157289","article-title":"Allelic variance between GRM6 mutants, Grm6nob3 and Grm6nob4 results in differences in retinal ganglion cell visual responses","volume":"586","author":"Maddox","year":"2008","journal-title":"J.\u00a0Physiol."},{"key":"10.1016\/j.jbc.2024.107119_bib26","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1523\/JNEUROSCI.3437-13.2014","article-title":"An unconventional secretory pathway mediates the cilia targeting of peripherin\/rds","volume":"34","author":"Tian","year":"2014","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.jbc.2024.107119_bib27","first-page":"310","article-title":"Prediction of glycosylation across the human proteome and the correlation to protein function","volume":"7","author":"Gupta","year":"2002","journal-title":"Pac. Symp. Biocomput."},{"key":"10.1016\/j.jbc.2024.107119_bib28","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1038\/363274a0","article-title":"Substitution of three amino acids switches receptor specificity of Gq\u03b1 to that of Gi\u03b1","volume":"363","author":"Conklin","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.107119_bib29","doi-asserted-by":"crossref","first-page":"29961","DOI":"10.1074\/jbc.M114.574483","article-title":"Selectivity and evolutionary divergence of metabotropic glutamate receptors for endogenous ligands and G proteins coupled to phospholipase C or TRP channels","volume":"289","author":"Kang","year":"2014","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.107119_bib30","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1073\/pnas.2235688100","article-title":"Electroporation and RNA interference in the rodent retina in\u00a0vivo and in\u00a0vitro","volume":"101","author":"Matsuda","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.107119_bib31","doi-asserted-by":"crossref","first-page":"7748","DOI":"10.1523\/JNEUROSCI.0397-08.2008","article-title":"A\u00a0core paired-type and POU homeodomain-containing transcription factor program drives retinal bipolar cell gene expression","volume":"28","author":"Kim","year":"2008","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.jbc.2024.107119_bib32","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/cne.24944","article-title":"LRRTM4 is a member of the transsynaptic complex between rod photoreceptors and bipolar cells","volume":"529","author":"Agosto","year":"2021","journal-title":"J.\u00a0Comp. Neurol."},{"key":"10.1016\/j.jbc.2024.107119_bib33","doi-asserted-by":"crossref","first-page":"27019","DOI":"10.1074\/jbc.M114.593780","article-title":"Oligomeric state of purified transient receptor potential melastatin-1 (TRPM1), a protein essential for dim light vision","volume":"289","author":"Agosto","year":"2014","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.107119_bib34","doi-asserted-by":"crossref","first-page":"14977","DOI":"10.1096\/fj.202001544R","article-title":"N-linked glycosylation of the mGlu7 receptor regulates the forward trafficking and transsynaptic interaction with Elfn1","volume":"34","author":"Park","year":"2020","journal-title":"FASEB J."},{"key":"10.1016\/j.jbc.2024.107119_bib35","doi-asserted-by":"crossref","first-page":"8763","DOI":"10.1073\/pnas.1807439115","article-title":"N-glycan microheterogeneity regulates interactions of plasma proteins","volume":"115","author":"Wu","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.107119_bib36","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/glycob\/cwz068","article-title":"A\u00a0synopsis of recent developments defining how N-glycosylation impacts immunoglobulin G structure and function","volume":"30","author":"Yamaguchi","year":"2020","journal-title":"Glycobiology"},{"key":"10.1016\/j.jbc.2024.107119_bib37","doi-asserted-by":"crossref","first-page":"216","DOI":"10.3389\/fendo.2018.00216","article-title":"In\u00a0Vivo and in\u00a0Vitro impact of carbohydrate variation on human follicle-stimulating hormone function","volume":"9","author":"Bousfield","year":"2018","journal-title":"Front. Endocrinol. (Lausanne)"},{"key":"10.1016\/j.jbc.2024.107119_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejcb.2021.151186","article-title":"Advances in understanding N-glycosylation structure, function, and regulation in health and disease","volume":"100","author":"Esmail","year":"2021","journal-title":"Eur. J. Cell Biol."},{"key":"10.1016\/j.jbc.2024.107119_bib39","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.neuron.2016.08.036","article-title":"Mechanism of assembly and cooperativity of homomeric and heteromeric metabotropic glutamate receptors","volume":"92","author":"Levitz","year":"2016","journal-title":"Neuron"},{"key":"10.1016\/j.jbc.2024.107119_bib40","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/s41586-019-0881-4","article-title":"Structural insights into the activation of metabotropic glutamate receptors","volume":"566","author":"Koehl","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.107119_bib41","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03495-2","article-title":"Structures of Gi-bound metabotropic glutamate receptors mGlu2 and mGlu4","volume":"594","author":"Lin","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.107119_bib42","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.bmcl.2018.01.037","article-title":"Determination of L-AP4-bound human mGlu8 receptor amino terminal domain structure and the molecular basis for L-AP4\u2019s group III mGlu receptor functional potency and selectivity","volume":"28","author":"Schkeryantz","year":"2018","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.jbc.2024.107119_bib43","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/s41586-021-03641-w","article-title":"Structures of human mGlu2 and mGlu7 homo- and heterodimers","volume":"594","author":"Du","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.107119_bib44","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1038\/s41422-022-00623-z","article-title":"Structural basis of the activation of metabotropic glutamate receptor 3","volume":"32","author":"Fang","year":"2022","journal-title":"Cell Res."},{"key":"10.1016\/j.jbc.2024.107119_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2021.109648","article-title":"Agonists and allosteric modulators promote signaling from different metabotropic glutamate receptor 5 conformations","volume":"36","author":"Nasrallah","year":"2021","journal-title":"Cell Rep."},{"key":"10.1016\/j.jbc.2024.107119_bib46","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/j.tins.2023.08.001","article-title":"Synapse organizers as molecular codes for synaptic plasticity","volume":"46","author":"Connor","year":"2023","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.jbc.2024.107119_bib47","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/bs.ircmb.2016.02.009","article-title":"Heparan sulfate: biosynthesis, structure, and function","volume":"325","author":"Li","year":"2016","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"10.1016\/j.jbc.2024.107119_bib48","doi-asserted-by":"crossref","DOI":"10.1523\/ENEURO.0143-18.2018","article-title":"A\u00a0large endoplasmic reticulum-resident pool of TRPM1 in retinal ON bipolar cells","volume":"5","author":"Agosto","year":"2018","journal-title":"eNeuro"},{"key":"10.1016\/j.jbc.2024.107119_bib49","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/S0092-8674(00)80877-6","article-title":"Neuroligin expressed in nonneuronal cells triggers presynaptic development in contacting axons","volume":"101","author":"Scheiffele","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2024.107119_bib50","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/nsmb.3014","article-title":"PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome","volume":"22","author":"Kroeze","year":"2015","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.jbc.2024.107119_bib51","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2113373118","article-title":"Recurrent high-impact mutations at cognate structural positions in class A G protein-coupled receptors expressed in tumors","volume":"118","author":"Huh","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.107119_bib52","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1167\/iovs.12-9662","article-title":"The rd8 mutation of the Crb1 gene is present in vendor lines of C57BL\/6N mice and embryonic stem cells, and confounds ocular induced mutant phenotypes","volume":"53","author":"Mattapallil","year":"2012","journal-title":"Invest. Ophthalmol. Vis. Sci."}],"updated-by":[{"DOI":"10.1016\/j.jbc.2024.107782","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000}}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925824016144?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925824016144?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T16:45:16Z","timestamp":1762188316000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925824016144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":52,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["S0021925824016144"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2024.107119","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Complex N-glycosylation of mGluR6 is required for trans-synaptic interaction with ELFN adhesion proteins","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2024.107119","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"107119"}}