{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T20:14:16Z","timestamp":1788984856927,"version":"build-2803163510"},"reference-count":74,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003792","name":"Brain Foundation","doi-asserted-by":"publisher","award":["ALZ2022-0004"],"award-info":[{"award-number":["ALZ2022-0004"]}],"id":[{"id":"10.13039\/501100003792","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003792","name":"Brain Foundation","doi-asserted-by":"publisher","award":["ALZ2019-0004"],"award-info":[{"award-number":["ALZ2019-0004"]}],"id":[{"id":"10.13039\/501100003792","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003792","name":"Brain Foundation","doi-asserted-by":"publisher","award":["FO2022-0072"],"award-info":[{"award-number":["FO2022-0072"]}],"id":[{"id":"10.13039\/501100003792","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003792","name":"Brain Foundation","doi-asserted-by":"publisher","award":["FO2020-0207"],"award-info":[{"award-number":["FO2020-0207"]}],"id":[{"id":"10.13039\/501100003792","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2016-00748"],"award-info":[{"award-number":["2016-00748"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2023-03275"],"award-info":[{"award-number":["2023-03275"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2021-01083"],"award-info":[{"award-number":["2021-01083"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2021-03524"],"award-info":[{"award-number":["2021-03524"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2023-03931"],"award-info":[{"award-number":["2023-03931"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006129","name":"King Gustaf V and Queen Victoria's Masonic Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018856","name":"Science and Engineering Faculty, Queensland University of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018856","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.jbc.2024.108032","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T23:02:26Z","timestamp":1732834946000},"page":"108032","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dual-ligand fluorescence microscopy enables chronological and spatial histological assignment of distinct amyloid-\u03b2 deposits"],"prefix":"10.1016","volume":"301","author":[{"given":"Ther\u00e9se","family":"Klingstedt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Shirani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1833-9697","authenticated-orcid":false,"given":"Farjana","family":"Parvin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofie","family":"Nystr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Hammarstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Graff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5466-8370","authenticated-orcid":false,"given":"Martin","family":"Ingelsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5803-3871","authenticated-orcid":false,"given":"Ruben","family":"Vidal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardino","family":"Ghetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-3859","authenticated-orcid":false,"given":"Dag","family":"Sehlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stina","family":"Syv\u00e4nen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5582-140X","authenticated-orcid":false,"given":"K. Peter. R.","family":"Nilsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2024.108032_bib1","first-page":"146","article-title":"\u00dcber eine eigenartige Erkrankung der Hirnrinde","volume":"64","author":"Alzheimer","year":"1907","journal-title":"Allg. Z. Psychiatr."},{"key":"10.1016\/j.jbc.2024.108032_bib2","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/S0006-291X(84)80190-4","article-title":"Alzheimer\u2019s disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein","volume":"120","author":"Glenner","year":"1984","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jbc.2024.108032_bib3","doi-asserted-by":"crossref","first-page":"4245","DOI":"10.1073\/pnas.82.12.4245","article-title":"Amyloid plaque core protein in Alzheimer disease and Down syndrome","volume":"82","author":"Masters","year":"1985","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.108032_bib4","doi-asserted-by":"crossref","first-page":"6084","DOI":"10.1016\/S0021-9258(17)38495-8","article-title":"Microtubule-associated protein tau. A component of Alzheimer paired helical filaments","volume":"261","author":"Grundke-Iqbal","year":"1986","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108032_bib5","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/0006-8993(88)90673-7","article-title":"Protein chemical and immunocytochemical studies of meningovascular \u03b2-amyloid protein in Alzheimer's disease and normal aging","volume":"474","author":"Joachim","year":"1988","journal-title":"Brain Res."},{"key":"10.1016\/j.jbc.2024.108032_bib6","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1111\/j.1471-4159.1988.tb01087.x","article-title":"Differences between vascular and plaque core amyloid in Alzheimer\u2019s disease","volume":"51","author":"Prelli","year":"1988","journal-title":"J.\u00a0Neurochem."},{"key":"10.1016\/j.jbc.2024.108032_bib7","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0896-6273(94)90458-8","article-title":"Visualization of A\u03b242(43) and A\u03b240 in senile plaques with end-specific A\u03b2 monoclonals: evidence that an initially deposited species is A\u03b242(43)","volume":"13","author":"Iwatsubo","year":"1993","journal-title":"Neuron"},{"key":"10.1016\/j.jbc.2024.108032_bib8","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1006\/abbi.1993.1112","article-title":"Peptide compositions of the cerebrovascular and senile plaque core amyloid deposits of Alzheimer's disease","volume":"301","author":"Miller","year":"1993","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.jbc.2024.108032_bib9","first-page":"1257","article-title":"Predominant deposition of amyloid-\u03b242(43) in plaques in cases of Alzheimer's disease and hereditary cerebral hemorrhage associated with mutations in the amyloid precursor protein gene","volume":"148","author":"Mann","year":"1996","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.jbc.2024.108032_bib10","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.neulet.2019.02.033","article-title":"A\u00a0distinct brain beta amyloid signature in cerebral amyloid angiopathy compared to Alzheimer\u2019s disease","volume":"701","author":"Gkanatsiou","year":"2019","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.jbc.2024.108032_bib11","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1126\/science.1105850","article-title":"Self-propagating, molecular-level polymorphism in Alzheimer\u2019s \u03b2-Amyloid Fibrils","volume":"307","author":"Petkova","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.108032_bib12","doi-asserted-by":"crossref","first-page":"18349","DOI":"10.1073\/pnas.0806270105","article-title":"Molecular structural basis for polymorphism in Alzheimer's beta-amyloid fibrils","volume":"105","author":"Paravastu","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.108032_bib13","doi-asserted-by":"crossref","first-page":"7462","DOI":"10.1073\/pnas.0712290105","article-title":"Paired beta-sheet structure of an Abeta(1-40) amyloid fibril revealed by electron microscopy","volume":"105","author":"Sachse","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.108032_bib14","doi-asserted-by":"crossref","first-page":"11858","DOI":"10.1073\/pnas.1503455112","article-title":"Peptide dimer structure in an A\u03b2(1-42) fibril visualized with cryo-EM","volume":"112","author":"Schmidt","year":"2015","journal-title":"Proc. Natl. Acad. Sc.i U. S. A."},{"key":"10.1016\/j.jbc.2024.108032_bib15","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1126\/science.aao2825","article-title":"Fibril structure of amyloid-\u03b2(1-42) by cryo-electron microscopy","volume":"358","author":"Gremer","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.108032_bib16","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1016\/j.cell.2013.08.035","article-title":"Molecular structure of \u03b2-amyloid fibrils in Alzheimer\u2019s disease brain tissue","volume":"154","author":"Lu","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2024.108032_bib17","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1038\/nature20814","article-title":"Structural variation in amyloid-\u03b2 fibrils from Alzheimer's disease clinical subtypes","volume":"541","author":"Qiang","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.108032_bib18","doi-asserted-by":"crossref","first-page":"4760","DOI":"10.1038\/s41467-019-12683-8","article-title":"Cryo-EM structure and polymorphism of A\u03b2 amyloid fibrils purified from Alzheimer\u2019s brain tissue","volume":"10","author":"Kollmer","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2024.108032_bib19","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1126\/science.abm7285","article-title":"Cryo-EM structures of amyloid-\u03b2 42 filaments from human brains","volume":"375","author":"Yang","year":"2022","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.108032_bib20","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s00401-022-02533-1","article-title":"Cryo-EM structures of amyloid-\u03b2 filaments with the Arctic mutation (E22G) from human and mouse brains","volume":"145","author":"Yang","year":"2023","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.jbc.2024.108032_bib21","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1186\/s40478-023-01694-8","article-title":"Cryo-EM structures of A\u03b240 filaments from the leptomeninges of individuals with Alzheimer's disease and cerebral amyloid angiopathy","volume":"11","author":"Yang","year":"2023","journal-title":"Acta Neuropathol. Commun."},{"key":"10.1016\/j.jbc.2024.108032_bib22","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1038\/s41593-023-01484-4","article-title":"Cryo-EM of A\u03b2 fibrils from mouse models find tg-APPArcSwe fibrils resemble those found in patients with sporadic Alzheimer's disease","volume":"26","author":"Zielinski","year":"2023","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.jbc.2024.108032_bib23","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/s41586-023-06437-2","article-title":"Molecular pathology of neurodegenerative diseases by cryo-EM of amyloids","volume":"621","author":"Scheres","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.108032_bib24","first-page":"1017","article-title":"Small organic probes as amyloid specific ligands--past and recent molecular scaffolds","volume":"14","author":"Nilsson","year":"2013","journal-title":"EMBO Rep."},{"key":"10.1016\/j.jbc.2024.108032_bib25","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1002\/ana.20009","article-title":"Imaging brain amyloid in Alzheimer's disease with Pittsburgh Compound-B","volume":"55","author":"Klunk","year":"2004","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.jbc.2024.108032_bib26","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1093\/brain\/awn016","article-title":"Post-mortem correlates of in\u00a0vivo PiB-PET amyloid imaging in a typical case of Alzheimer\u2019s disease","volume":"131","author":"Ikonomovic","year":"2008","journal-title":"Brain"},{"key":"10.1016\/j.jbc.2024.108032_bib27","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.neuint.2008.12.016","article-title":"[11C] PIB-amyloid binding and levels of A\u03b240 and A\u03b242 in postmortem brain tissue from Alzheimer patients","volume":"54","author":"Svedberg","year":"2009","journal-title":"Neurochem. Int."},{"key":"10.1016\/j.jbc.2024.108032_bib28","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s00401-009-0583-3","article-title":"Deficient high-affinity binding of Pittsburgh compound B in a case of Alzheimer\u2019s disease","volume":"119","author":"Rosen","year":"2010","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.jbc.2024.108032_bib29","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/nm0498-452","article-title":"A\u00a0variant of Alzheimer's disease with spastic paraparesis and unusual plaques due to deletion of exon 9 of presenilin 1","volume":"4","author":"Crook","year":"1998","journal-title":"Nat. Med."},{"key":"10.1016\/j.jbc.2024.108032_bib30","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1750-1326-3-20","article-title":"Histopathological and molecular heterogeneity among individuals with dementia associated with Presenilin mutations","volume":"3","author":"Maarouf","year":"2008","journal-title":"Mol. Neurodegener."},{"key":"10.1016\/j.jbc.2024.108032_bib31","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1093\/brain\/awab434","article-title":"11C-PiB PET can underestimate brain amyloid-\u03b2 burden when cotton wool plaques are numerous","volume":"145","author":"Abrahamson","year":"2021","journal-title":"Brain"},{"key":"10.1016\/j.jbc.2024.108032_bib32","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1021\/cb4000376","article-title":"Evidence for age-dependent in\u00a0vivo conformational rearrangement within A\u03b2 amyloid deposits","volume":"8","author":"Nystr\u00f6m","year":"2013","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.jbc.2024.108032_bib33","doi-asserted-by":"crossref","first-page":"9072","DOI":"10.1002\/chem.201500556","article-title":"Distinct spacing between anionic groups: an essential chemical determinant for achieving thiophene-based ligands to distinguish \u03b2-amyloid or tau polymorphic aggregates","volume":"21","author":"Klingstedt","year":"2015","journal-title":"Chemistry"},{"key":"10.1016\/j.jbc.2024.108032_bib34","doi-asserted-by":"crossref","first-page":"13018","DOI":"10.1073\/pnas.1713215114","article-title":"Amyloid polymorphisms constitute distinct clouds of conformational variants in different etiological subtypes of Alzheimer\u2019s disease","volume":"114","author":"Rasmussen","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.108032_bib35","doi-asserted-by":"crossref","first-page":"E782","DOI":"10.1073\/pnas.1714966115","article-title":"Structural heterogeneity and intersubject variability of A\u03b2 in familial and sporadic Alzheimer's disease","volume":"115","author":"Condello","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.108032_bib36","doi-asserted-by":"crossref","first-page":"6719","DOI":"10.1074\/jbc.RA118.006604","article-title":"Pyroglutamation of amyloid-\u03b2x-42 (A\u03b2x-42) followed by A\u03b21-40 deposition underlies plaque polymorphism in progressing Alzheimer's disease pathology","volume":"294","author":"Michno","year":"2019","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108032_bib37","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1186\/s40478-021-01298-0","article-title":"Emergence of distinct and heterogeneous strains of amyloid beta with advanced Alzheimer's disease pathology in Down syndrome","volume":"9","author":"Maxwell","year":"2021","journal-title":"Acta Neuropathol. Commun."},{"key":"10.1016\/j.jbc.2024.108032_bib38","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1186\/s40478-022-01456-y","article-title":"Dual-probe fluorescence spectroscopy for sensitive quantitation of Alzheimer's amyloid pathology","volume":"10","author":"Stepanchuk","year":"2022","journal-title":"Acta Neuropathol. Commun."},{"key":"10.1016\/j.jbc.2024.108032_bib39","article-title":"Thiophene-based ligands for histological multiplex spectral detection of distinct protein aggregates in Alzheimer's disease","volume":"29","author":"Lantz","year":"2023","journal-title":"Chemistry"},{"key":"10.1016\/j.jbc.2024.108032_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.jphotobiol.2023.112786","article-title":"Amyloid dye pairs as spectral sensors for enhanced detection and differentiation of misfolded proteins","volume":"248","author":"Stepanchuk","year":"2023","journal-title":"J.\u00a0Photochem. Photobiol. B."},{"key":"10.1016\/j.jbc.2024.108032_bib41","article-title":"EMBER multidimensional spectral microscopy enables quantitative determination of disease- and cell-specific amyloid strains","volume":"120","author":"Yang","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2024.108032_bib42","doi-asserted-by":"crossref","first-page":"12537","DOI":"10.1002\/chem.201402890","article-title":"Pentameric thiophene-based ligands that spectrally discriminate amyloid-\u03b2 and tau aggregates display distinct solvatochromism and viscosity-induced spectral shifts","volume":"20","author":"Simon","year":"2014","journal-title":"Chemistry"},{"key":"10.1016\/j.jbc.2024.108032_bib43","doi-asserted-by":"crossref","first-page":"15133","DOI":"10.1002\/chem.201502999","article-title":"A\u00a0palette of fluorescent thiophene-based ligands for the identification of protein aggregates","volume":"21","author":"Shirani","year":"2005","journal-title":"Chemistry"},{"key":"10.1016\/j.jbc.2024.108032_bib44","doi-asserted-by":"crossref","DOI":"10.1002\/ejoc.202300583","article-title":"Distinct heterocyclic moieties govern the selectivity of thiophene-vinylene-based ligands towards A\u03b2 or tau pathology in Alzheimer's disease","volume":"26","author":"Bj\u00f6rk","year":"2023","journal-title":"Eur. J. Org. Chem."},{"key":"10.1016\/j.jbc.2024.108032_bib45","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1002\/cbic.202100199","article-title":"Thiophene-based optical ligands that selectively detect A\u03b2 pathology in Alzheimer's disease","volume":"22","author":"Klingstedt","year":"2021","journal-title":"Chembiochem."},{"key":"10.1016\/j.jbc.2024.108032_bib46","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aab1923","article-title":"Structure-based drug design identifies polythiophenes as antiprion compounds","volume":"7","author":"Herrmann","year":"2015","journal-title":"Sci. Transl Med."},{"key":"10.1016\/j.jbc.2024.108032_bib47","doi-asserted-by":"crossref","first-page":"13287","DOI":"10.1073\/pnas.94.24.13287","article-title":"Two amyloid precursor protein transgenic mouse models with Alzheimer disease-like pathology","volume":"94","author":"Sturchler-Pierrat","year":"1997","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108032_bib48","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.neurobiolaging.2004.12.007","article-title":"The Arctic Alzheimer mutation facilitates early intraneuronal Abeta aggregation and senile plaque formation in transgenic mice","volume":"27","author":"Lord","year":"2006","journal-title":"Neurobiol. Aging"},{"key":"10.1016\/j.jbc.2024.108032_bib49","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/nn.3697","article-title":"Single App knock-in mouse models of Alzheimer's disease","volume":"17","author":"Saito","year":"2014","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.jbc.2024.108032_bib50","first-page":"1234","article-title":"Epitomic characterization of the specificity of the anti-amyloid A\u03b2 monoclonal antibodies 6E10 and 4G8","volume":"66","author":"Baghallab","year":"2018","journal-title":"J.\u00a0Alzheimers Dis."},{"key":"10.1016\/j.jbc.2024.108032_bib51","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1212\/WNL.57.4.621","article-title":"Screening for PS1 mutations in a referral-based series of AD cases: 21 novel mutations","volume":"57","author":"Rogaeva","year":"2001","journal-title":"Neurology"},{"key":"10.1016\/j.jbc.2024.108032_bib52","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s10048-006-0043-3","article-title":"Founder effect for the Ala431Glu mutation of the presenilin 1 gene causing early-onset Alzheimer's disease in Mexican families","volume":"7","author":"Yescas","year":"2006","journal-title":"Neurogenetics"},{"key":"10.1016\/j.jbc.2024.108032_bib53","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s10048-006-0053-1","article-title":"The A431E mutation in PSEN1 causing Familial Alzheimer\u2019s Disease originating in Jalisco state, Mexico: an additional fifteen families","volume":"7","author":"Murrell","year":"2006","journal-title":"Neurogenetics"},{"key":"10.1016\/j.jbc.2024.108032_bib54","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1038\/nn0901-887","article-title":"The \u2018Arctic\u2019 APP mutation (E693G) causes Alzheimer\u2019s disease by enhanced A\u00df protofibril formation","volume":"4","author":"Nilsberth","year":"2001","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.jbc.2024.108032_bib55","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1001\/archneur.65.4.499","article-title":"Clinical and neuropathological features of the Arctic APP mutation causing early onset Alzheimer's disease","volume":"65","author":"Basun","year":"2008","journal-title":"Arch. Neurol."},{"key":"10.1016\/j.jbc.2024.108032_bib56","doi-asserted-by":"crossref","first-page":"10810","DOI":"10.1021\/bi0508237","article-title":"Amino-terminally truncated A\u03b2 peptide species are the main component of cotton wool plaques","volume":"44","author":"Miravalle","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbc.2024.108032_bib57","doi-asserted-by":"crossref","first-page":"1010.e1","DOI":"10.1016\/j.neurobiolaging.2011.10.022","article-title":"The Arctic amyloid-\u03b2 precursor protein (A\u03b2PP) mutation results in distinct plaques and accumulation of N- and C-truncated A\u03b2","volume":"33","author":"Philipson","year":"2012","journal-title":"Neurobiol. Aging"},{"key":"10.1016\/j.jbc.2024.108032_bib58","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/2051-5960-1-60","article-title":"The Arctic A\u03b2PP mutation leads to Alzheimer's disease pathology with highly variable topographic deposition of differentially truncated A\u03b2","volume":"1","author":"Kalimo","year":"2013","journal-title":"Acta. Neuropathol. Commun."},{"key":"10.1016\/j.jbc.2024.108032_bib59","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.2967\/jnumed.109.063305","article-title":"Phase 1 study of the Pittsburgh compound B derivative 18F-flutemetamol in healthy volunteers and patients with probable Alzheimer disease","volume":"50","author":"Nelissen","year":"2009","journal-title":"J.\u00a0Nucl. Med."},{"key":"10.1016\/j.jbc.2024.108032_bib60","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1002\/ana.22068","article-title":"18F-flutemetamol amyloid imaging in Alzheimer disease and mild cognitive impairment: a phase 2 trial","volume":"68","author":"Vandenberghe","year":"2010","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.jbc.2024.108032_bib61","doi-asserted-by":"crossref","first-page":"197","DOI":"10.14283\/jpad.2022.30","article-title":"Two randomized phase 3 studies of aducanumab in early Alzheimer's disease","volume":"9","author":"Budd Haeberlein","year":"2022","journal-title":"J.\u00a0Prev. Alzheimers Dis."},{"key":"10.1016\/j.jbc.2024.108032_bib62","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1056\/NEJMoa2212948","article-title":"Lecanemab in early Alzheimer's disease","volume":"388","author":"van Dyck","year":"2023","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.jbc.2024.108032_bib63","article-title":"Donanemab in early symptomatic Alzheimer disease: the TRAILBLAZER-ALZ 2 randomized clinical trial","volume":"6","author":"Sims","year":"2023","journal-title":"JAMA Netw. Open"},{"key":"10.1016\/j.jbc.2024.108032_bib64","doi-asserted-by":"crossref","first-page":"3815","DOI":"10.1002\/pmic.200700311","article-title":"Urea-based two-dimensional electrophoresis of beta-amyloid peptides in human plasma: evidence for novel Abeta species","volume":"7","author":"Maler","year":"2007","journal-title":"Proteomics"},{"key":"10.1016\/j.jbc.2024.108032_bib65","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.neuroscience.2006.08.027","article-title":"High sensitivity analysis of amyloid-beta peptide composition in amyloid deposits from human and PS2APP mouse brain","volume":"143","author":"Guntert","year":"2006","journal-title":"Neuroscience"},{"key":"10.1016\/j.jbc.2024.108032_bib66","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1159\/000203774","article-title":"Identification of novel APP\/Abeta isoforms in human cerebrospinal fluid","volume":"6","author":"Portelius","year":"2009","journal-title":"Neurodegener. Dis."},{"key":"10.1016\/j.jbc.2024.108032_bib67","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.202216789","article-title":"Whole-brain microscopy reveals distinct temporal and spatial efficacy of anti-A\u03b2 therapies","volume":"15","author":"Kirschenbaum","year":"2023","journal-title":"EMBO Mol. Med."},{"key":"10.1016\/j.jbc.2024.108032_bib68","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1186\/s40478-019-0832-1","article-title":"In\u00a0vivo detection of tau fibrils and amyloid \u03b2 aggregates with luminescent conjugated oligothiophenes and multiphoton microscopy","volume":"7","author":"Calvo-Rodriguez","year":"2019","journal-title":"Acta Neuropathol. Commun."},{"key":"10.1016\/j.jbc.2024.108032_bib69","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1021\/acschemneuro.4c00021","article-title":"Thiophene-based ligands for specific assignment of distinct A\u03b2 pathologies in Alzheimer's disease","volume":"15","author":"Klingstedt","year":"2024","journal-title":"ACS Chem. Neurosci."},{"key":"10.1016\/j.jbc.2024.108032_bib70","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1212\/WNL.0b013e31825fdf18","article-title":"Low PiB PET retention in presence of pathologic CSF biomarkers in Arctic APP mutation carriers","volume":"79","author":"Sch\u00f6ll","year":"2012","journal-title":"Neurology"},{"key":"10.1016\/j.jbc.2024.108032_bib71","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/s41586-020-1984-7","article-title":"Discriminating \u03b1-synuclein strains in Parkinson's disease and multiple system atrophy","volume":"578","author":"Shahnawaz","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.108032_bib72","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.celrep.2014.02.031","article-title":"Detection of misfolded A\u03b2 oligomers for sensitive biochemical diagnosis of Alzheimer's disease","volume":"7","author":"Salvadores","year":"2014","journal-title":"Cell Rep."},{"key":"10.1016\/j.jbc.2024.108032_bib73","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1021\/acsmedchemlett.5b00309","article-title":"(11)C and (18)F radiolabeling of tetra- and pentathiophenes as PET-ligands for amyloid protein aggregates","volume":"7","author":"Nordeman","year":"2016","journal-title":"ACS Med. Chem. Lett."},{"key":"10.1016\/j.jbc.2024.108032_bib75","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1038\/s41467-023-38495-5","article-title":"The in-tissue molecular architecture of \u03b2-amyloid pathology in the mammalian brain","volume":"14","author":"Leistner","year":"2023","journal-title":"Nat. Commun."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925824025341?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925824025341?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T19:35:17Z","timestamp":1788982517000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925824025341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":74,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["S0021925824025341"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2024.108032","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dual-ligand fluorescence microscopy enables chronological and spatial histological assignment of distinct amyloid-\u03b2 deposits","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2024.108032","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"108032"}}