{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T19:34:39Z","timestamp":1793388879575,"version":"4.5.0"},"reference-count":125,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.jbc.2024.108079","type":"journal-article","created":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T02:55:45Z","timestamp":1734144945000},"page":"108079","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["The zymogenic form of SARS-CoV-2 main protease: A discrete target for drug discovery"],"prefix":"10.1016","volume":"301","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7325-6961","authenticated-orcid":false,"given":"Pavel","family":"Novotn\u00fd","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Humpol\u00ed\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Nov\u00e1kov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stancho","family":"Stanchev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kvido","family":"St\u0159\u00ed\u0161ovsk\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michala","family":"Zgarbov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Kry\u0161t\u016ffek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Starkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Hradilek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ad\u00e9la","family":"Moravcov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"G\u00fcnterov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Bach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Majer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Konvalinka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5536-3587","authenticated-orcid":false,"given":"Ta\u0165\u00e1na","family":"Majerov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2024.108079_bib1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/s41579-020-00468-6","article-title":"Coronavirus biology and replication: implications for SARS-CoV-2","volume":"19","author":"V'Kovski","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jbc.2024.108079_bib2","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1021\/acschembio.1c00324","article-title":"The SARS-CoV-2 programmed -1 ribosomal frameshifting element crystal structure solved to 2.09 \u00c5 using chaperone-assisted RNA crystallography","volume":"16","author":"Roman","year":"2021","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib3","doi-asserted-by":"crossref","first-page":"4330","DOI":"10.1128\/JVI.02480-09","article-title":"Achieving a golden mean: mechanisms by which coronaviruses ensure synthesis of the correct stoichiometric ratios of viral proteins","volume":"84","author":"Plant","year":"2010","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib4","article-title":"Structural basis for replicase polyprotein cleavage and substrate specificity of main protease from SARS-CoV-2","volume":"119","author":"Zhao","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108079_bib5","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1089\/cmb.2020.0627","article-title":"Mapping the nonstructural transmembrane proteins of severe acute respiratory syndrome coronavirus 2","volume":"28","author":"Thomas","year":"2021","journal-title":"J.\u00a0Comput. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib6","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41580-021-00432-z","article-title":"Structures and functions of coronavirus replication-transcription complexes and their relevance for SARS-CoV-2 drug design","volume":"23","author":"Malone","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol"},{"key":"10.1016\/j.jbc.2024.108079_bib7","doi-asserted-by":"crossref","first-page":"E3900","DOI":"10.1073\/pnas.1323705111","article-title":"One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities","volume":"111","author":"Subissi","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108079_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2024.107367","article-title":"Kinetic comparison of all eleven viral polyprotein cleavage site processing events by SARS-CoV-2 main protease using a linked protein FRET platform","volume":"300","author":"Kenward","year":"2024","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib9","article-title":"Biochemical and structural insights into SARS-CoV-2 polyprotein processing","volume":"8","author":"Yadav","year":"2022","journal-title":"Mpro Sci. Adv."},{"key":"10.1016\/j.jbc.2024.108079_bib10","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1042\/BCJ20200029","article-title":"Processing of the SARS-CoV pp1a\/ab nsp7-10 region","volume":"477","author":"Krichel","year":"2020","journal-title":"Biochem. J."},{"key":"10.1016\/j.jbc.2024.108079_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2023.104697","article-title":"SARS-CoV-2 polyprotein substrate regulates the stepwise M(pro) cleavage reaction","volume":"299","author":"Narwal","year":"2023","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib12","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1021\/acschembio.4c00096","article-title":"High-resolution substrate specificity profiling of SARS-CoV-2 M(pro); comparison to SARS-CoV M(pro)","volume":"19","author":"Yaghi","year":"2024","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2021.167118","article-title":"A\u00a0crystallographic snapshot of SARS-CoV-2 main protease maturation process","volume":"433","author":"Noske","year":"2021","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib14","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abe0751","article-title":"Structure and inhibition of the SARS-CoV-2 main protease reveal strategy for developing dual inhibitors against M(pro) and cathepsin L","volume":"6","author":"Sacco","year":"2020","journal-title":"Sci. Adv."},{"key":"10.1016\/j.jbc.2024.108079_bib15","doi-asserted-by":"crossref","first-page":"7894","DOI":"10.1038\/s41467-023-43666-5","article-title":"SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelle","volume":"14","author":"Zimmermann","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2024.108079_bib16","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.87884.3","article-title":"Emerging variants of SARS-CoV-2 NSP10 highlight strong functional conservation of its binding to two non-structural proteins, NSP14 and NSP16","volume":"12","author":"Wang","year":"2023","journal-title":"Elife"},{"key":"10.1016\/j.jbc.2024.108079_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbagen.2023.130319","article-title":"SS148 and WZ16 inhibit the activities of nsp10-nsp16 complexes from all seven human pathogenic coronaviruses","volume":"1867","author":"Li","year":"2023","journal-title":"Biochim. Biophys. Acta Gen. Subj"},{"key":"10.1016\/j.jbc.2024.108079_bib18","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1126\/science.abi9310","article-title":"Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme","volume":"373","author":"Liu","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.108079_bib19","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1093\/nar\/gkab1279","article-title":"Coronaviral RNA-methyltransferases: function, structure and inhibition","volume":"50","author":"Nencka","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jbc.2024.108079_bib20","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02335-21","article-title":"SARS-CoV-2 Nsp5 demonstrates two distinct mechanisms targeting RIG-I and MAVS to evade the innate immune response","volume":"12","author":"Liu","year":"2021","journal-title":"mBio"},{"key":"10.1016\/j.jbc.2024.108079_bib21","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1021\/acscentsci.3c00054","article-title":"SARS-CoV-2 M(pro) protease variants of concern display altered viral substrate and cell host target galectin-8 processing but retain sensitivity toward antivirals","volume":"9","author":"Chen","year":"2023","journal-title":"ACS Cent. Sci."},{"key":"10.1016\/j.jbc.2024.108079_bib22","article-title":"SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense","volume":"14","author":"Ju","year":"2023","journal-title":"mBio"},{"key":"10.1016\/j.jbc.2024.108079_bib23","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/s41586-020-2601-5","article-title":"Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity","volume":"587","author":"Shin","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.108079_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2021.109892","article-title":"Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CL(pro) substrate degradome","volume":"37","author":"Pablos","year":"2021","journal-title":"Cell Rep"},{"key":"10.1016\/j.jbc.2024.108079_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2023.102990","article-title":"The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes","volume":"299","author":"Song","year":"2023","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib26","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02374-20","article-title":"Inhibitors of coronavirus 3CL proteases protect cells from protease-mediated cytotoxicity","volume":"95","author":"Resnick","year":"2021","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib27","article-title":"Gain-of-Signal assays for probing inhibition of SARS-CoV-2 M(pro)\/3CL(pro)","volume":"13","author":"Moghadasi","year":"2022","journal-title":"Living. Cells. mBio"},{"key":"10.1016\/j.jbc.2024.108079_bib28","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1021\/acscentsci.1c00910","article-title":"Evaluation of SARS-CoV-2 main protease inhibitors using a novel cell-based assay","volume":"8","author":"Cao","year":"2022","journal-title":"ACS Cent. Sci."},{"key":"10.1016\/j.jbc.2024.108079_bib29","doi-asserted-by":"crossref","first-page":"4259","DOI":"10.1074\/jbc.RA118.006780","article-title":"Polyprotein processing and intermolecular interactions within the viral replication complex spatially and temporally control norovirus protease activity","volume":"294","author":"Emmott","year":"2019","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib30","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.coviro.2013.03.009","article-title":"Viral precursor polyproteins: keys of regulation from replication to maturation","volume":"3","author":"Yost","year":"2013","journal-title":"Curr. Opin. Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib31","doi-asserted-by":"crossref","first-page":"16534","DOI":"10.1073\/pnas.1210418109","article-title":"Structural and functional insights into alphavirus polyprotein processing and pathogenesis","volume":"109","author":"Shin","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108079_bib32","doi-asserted-by":"crossref","first-page":"13722","DOI":"10.1128\/JVI.02271-14","article-title":"Induced maturation of human immunodeficiency virus","volume":"88","author":"Mattei","year":"2014","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib33","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.1128\/JVI.72.4.2846-2854.1998","article-title":"Sequential steps in human immunodeficiency virus particle maturation revealed by alterations of individual Gag polyprotein cleavage sites","volume":"72","author":"Wiegers","year":"1998","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib34","doi-asserted-by":"crossref","first-page":"3213","DOI":"10.1073\/pnas.88.8.3213","article-title":"Human immunodeficiency virus proteinase dimer as component of the viral polyprotein prevents particle assembly and viral infectivity","volume":"88","author":"Kr\u00e4usslich","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108079_bib35","doi-asserted-by":"crossref","DOI":"10.1096\/fj.202002271","article-title":"Spatial and temporal roles of SARS-CoV PL(pro)-A snapshot","volume":"35","author":"Yan","year":"2021","journal-title":"Faseb j"},{"key":"10.1016\/j.jbc.2024.108079_bib36","doi-asserted-by":"crossref","first-page":"12611","DOI":"10.1128\/JVI.02050-13","article-title":"Chimeric exchange of coronavirus nsp5 proteases (3CLpro) identifies common and divergent regulatory determinants of protease activity","volume":"87","author":"Stobart","year":"2013","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib37","article-title":"Timeliness of proteolytic events is prerequisite for efficient functioning of the alphaviral","volume":"92","author":"Lulla","year":"2018","journal-title":"Replicase J. Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib38","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00707-22","article-title":"Selective polyprotein processing determines norovirus sensitivity to Trim7","volume":"96","author":"Sullender","year":"2022","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib39","doi-asserted-by":"crossref","first-page":"2631","DOI":"10.1002\/j.1460-2075.1990.tb07445.x","article-title":"Cleavage-site preferences of Sindbis virus polyproteins containing the non-structural proteinase. Evid. temporal Regul. polyprotein Process. vivo","volume":"9","author":"de Groot","year":"1990","journal-title":"EMBO J."},{"key":"10.1016\/j.jbc.2024.108079_bib40","doi-asserted-by":"crossref","first-page":"9746","DOI":"10.1128\/JVI.79.15.9746-9755.2005","article-title":"Persistence of bovine viral diarrhea virus is determined by a cellular cofactor of a viral autoprotease","volume":"79","author":"Lackner","year":"2005","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib41","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.1002\/j.1460-2075.1994.tb06587.x","article-title":"Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus- and plus-strand RNA synthesis","volume":"13","author":"Lemm","year":"1994","journal-title":"EMBO J."},{"key":"10.1016\/j.jbc.2024.108079_bib42","doi-asserted-by":"crossref","first-page":"3428","DOI":"10.1128\/JVI.80.7.3428-3437.2006","article-title":"Mutagenesis analysis of the nsp4 main proteinase reveals determinants of arterivirus replicase polyprotein autoprocessing","volume":"80","author":"van Aken","year":"2006","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib43","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0300-9084(88)90166-6","article-title":"Polyprotein processing in picornavirus replication","volume":"70","author":"Kr\u00e4usslich","year":"1988","journal-title":"Biochimie"},{"key":"10.1016\/j.jbc.2024.108079_bib44","doi-asserted-by":"crossref","first-page":"10280","DOI":"10.1128\/JVI.00017-07","article-title":"Processing of open reading frame 1a replicase proteins nsp7 to nsp10 in murine hepatitis virus strain A59 replication","volume":"81","author":"Deming","year":"2007","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib45","doi-asserted-by":"crossref","first-page":"6080","DOI":"10.1038\/s41467-024-50437-3","article-title":"Pan-serotype dengue virus inhibitor JNJ-A07 targets NS4A-2K-NS4B interaction with NS2B\/NS3 and blocks replication organelle formation","volume":"15","author":"Kiemel","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2024.108079_bib46","doi-asserted-by":"crossref","first-page":"27015","DOI":"10.1016\/S0021-9258(18)47119-0","article-title":"Stimulation of poliovirus proteinase 3Cpro-related proteolysis by the genome-linked protein VPg and its precursor 3AB","volume":"269","author":"Molla","year":"1994","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib47","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1038\/s41573-023-00672-y","article-title":"Therapeutic strategies for COVID-19: progress and lessons learned","volume":"22","author":"Li","year":"2023","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jbc.2024.108079_bib48","doi-asserted-by":"crossref","first-page":"797","DOI":"10.3390\/biom14070797","article-title":"Inhibitors of SARS-CoV-2 main protease (Mpro) as anti-coronavirus Agents","volume":"14","author":"Zag\u00f3rska","year":"2024","journal-title":"Biomolecules"},{"key":"10.1016\/j.jbc.2024.108079_bib49","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1126\/science.abb3405","article-title":"Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved \u03b1-ketoamide inhibitors","volume":"368","author":"Zhang","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.108079_bib50","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/s41586-020-2223-y","article-title":"Structure of M(pro) from SARS-CoV-2 and discovery of its inhibitors","volume":"582","author":"Jin","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2024.108079_bib51","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/s41392-022-00884-5","article-title":"Structural biology of SARS-CoV-2: open the door for novel therapies","volume":"7","author":"Yan","year":"2022","journal-title":"Signal. Transduct. Target. Ther."},{"key":"10.1016\/j.jbc.2024.108079_bib52","doi-asserted-by":"crossref","first-page":"3336","DOI":"10.1016\/j.csbj.2022.06.023","article-title":"Maturation of the SARS-CoV-2 virus is regulated by dimerization of its main protease","volume":"20","author":"Kaptan","year":"2022","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"10.1016\/j.jbc.2024.108079_bib53","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02094-21","article-title":"Regulation of the dimerization and activity of SARS-CoV-2 main protease through reversible glutathionylation of cysteine 300","volume":"12","author":"Davis","year":"2021","journal-title":"mBio"},{"key":"10.1016\/j.jbc.2024.108079_bib54","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.131392","article-title":"SARS-CoV-2 M(pro) oligomerization as a potential target for therapy","volume":"267","author":"Lis","year":"2024","journal-title":"Int. J. Biol. Macromol"},{"key":"10.1016\/j.jbc.2024.108079_bib55","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1038\/s42003-022-03084-7","article-title":"Modulation of the monomer-dimer equilibrium and catalytic activity of SARS-CoV-2 main protease by a transition-state analog inhibitor","volume":"5","author":"Nashed","year":"2022","journal-title":"Commun. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib56","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1038\/s42003-022-03910-y","article-title":"Autoprocessing and oxyanion loop reorganization upon GC373 and nirmatrelvir binding of monomeric SARS-CoV-2 main protease catalytic domain","volume":"5","author":"Nashed","year":"2022","journal-title":"Commun. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib57","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s13238-010-0011-4","article-title":"Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode","volume":"1","author":"Chen","year":"2010","journal-title":"Protein Cell"},{"key":"10.1016\/j.jbc.2024.108079_bib58","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2024.168616","article-title":"Visualizing the active site oxyanion loop transition upon ensitrelvir binding and transient dimerization of SARS-CoV-2 main protease","volume":"436","author":"Kovalevsky","year":"2024","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib59","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1038\/s42003-023-05469-8","article-title":"Insights into the mechanism of SARS-CoV-2 main protease autocatalytic maturation from model precursors","volume":"6","author":"Aniana","year":"2023","journal-title":"Commun. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib60","doi-asserted-by":"crossref","first-page":"31257","DOI":"10.1074\/jbc.M502577200","article-title":"Mechanism of the maturation process of SARS-CoV 3CL protease","volume":"280","author":"Hsu","year":"2005","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib61","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1111\/febs.12222","article-title":"Autoprocessing mechanism of severe acute respiratory syndrome coronavirus 3C-like protease (SARS-CoV 3CLpro) from its polyproteins","volume":"280","author":"Muramatsu","year":"2013","journal-title":"Febs J"},{"key":"10.1016\/j.jbc.2024.108079_bib62","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1101\/gr.849004","article-title":"WebLogo: a sequence logo generator","volume":"14","author":"Crooks","year":"2004","journal-title":"Genome Res."},{"key":"10.1016\/j.jbc.2024.108079_bib63","doi-asserted-by":"crossref","DOI":"10.1016\/j.mam.2022.101159","article-title":"Viral proteases as therapeutic targets","volume":"88","author":"Majerov\u00e1","year":"2022","journal-title":"Mol. Aspects. Med."},{"key":"10.1016\/j.jbc.2024.108079_bib64","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1056\/NEJMoa2301425","article-title":"Oral simnotrelvir for adult patients with mild-to-moderate covid-19","volume":"390","author":"Cao","year":"2024","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.jbc.2024.108079_bib65","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1080\/17460441.2023.2267001","article-title":"Lessons learnt from the preclinical discovery and development of ensitrelvir as a COVID-19 therapeutic option","volume":"19","author":"Ferraro","year":"2024","journal-title":"Expert Opin. Drug Discov."},{"key":"10.1016\/j.jbc.2024.108079_bib66","doi-asserted-by":"crossref","first-page":"9072","DOI":"10.1073\/pnas.1102278108","article-title":"Inhibition of autoprocessing of natural variants and multidrug resistant mutant precursors of HIV-1 protease by clinical inhibitors","volume":"108","author":"Louis","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108079_bib67","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-28638-w","article-title":"Inhibition of the precursor and mature forms of HIV-1 protease as a tool for drug evaluation","volume":"8","author":"Humpol\u00ed\u010dkov\u00e1","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbc.2024.108079_bib68","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00928-23","article-title":"Differential inhibition of intra- and inter-molecular protease cleavages by antiviral compounds","volume":"97","author":"Doherty","year":"2023","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib69","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.3390\/v13101981","article-title":"Precursors of viral proteases as distinct drug targets","volume":"13","author":"Majerov\u00e1","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.jbc.2024.108079_bib70","doi-asserted-by":"crossref","first-page":"10136","DOI":"10.1073\/pnas.1805195115","article-title":"Targeting intramolecular proteinase NS2B\/3 cleavages for trans-dominant inhibition of dengue virus","volume":"115","author":"Constant","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108079_bib71","doi-asserted-by":"crossref","first-page":"29692","DOI":"10.1074\/jbc.M109.027144","article-title":"HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity","volume":"284","author":"M\u00fcller","year":"2009","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib72","article-title":"Placement of leucine zipper motifs at the carboxyl terminus of HIV-1 protease significantly reduces virion production","volume":"7","author":"Pan","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.jbc.2024.108079_bib73","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.virol.2008.10.038","article-title":"Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly","volume":"384","author":"Z\u00e1bransk\u00fd","year":"2009","journal-title":"Virology"},{"key":"10.1016\/j.jbc.2024.108079_bib74","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s12977-019-0479-9","article-title":"NNRTI-induced HIV-1 protease-mediated cytotoxicity induces rapid death of CD4 T cells during productive infection and latency reversal","volume":"16","author":"Trinit\u00e9","year":"2019","journal-title":"Retrovirology"},{"key":"10.1016\/j.jbc.2024.108079_bib75","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/1742-4690-7-89","article-title":"Selective killing of human immunodeficiency virus infected cells by non-nucleoside reverse transcriptase inhibitor-induced activation of HIV protease","volume":"7","author":"Jochmans","year":"2010","journal-title":"Retrovirology"},{"key":"10.1016\/j.jbc.2024.108079_bib76","doi-asserted-by":"crossref","first-page":"3348","DOI":"10.1128\/JVI.02306-12","article-title":"Efavirenz enhances HIV-1 gag processing at the plasma membrane through Gag-Pol dimerization","volume":"87","author":"Sudo","year":"2013","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib77","doi-asserted-by":"crossref","DOI":"10.1126\/science.abe1707","article-title":"CARD8 is an inflammasome sensor for HIV-1 protease activity","volume":"371","author":"Wang","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.108079_bib78","doi-asserted-by":"crossref","first-page":"5306","DOI":"10.1073\/pnas.0700184104","article-title":"Utilizing the activation mechanism of serine proteases to engineer hepatocyte growth factor into a Met antagonist","volume":"104","author":"Kirchhofer","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108079_bib79","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/s42003-020-01266-9","article-title":"Over-activation of a nonessential bacterial protease DegP as an antibiotic strategy","volume":"3","author":"Cho","year":"2020","journal-title":"Commun. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib80","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/0014-5793(76)80443-7","article-title":"Induction of the bovine trypsinogen-trypsin transition by peptides sequentially similar to the N-terminus of trypsin","volume":"68","author":"Bode","year":"1976","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jbc.2024.108079_bib81","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1038\/s41589-018-0222-1","article-title":"An allosteric MALT1 inhibitor is a molecular corrector rescuing function in an immunodeficient patient","volume":"15","author":"Quancard","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib82","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1128\/AAC.45.9.2616-2622.2001","article-title":"Cell-based fluorescence assay for human immunodeficiency virus type 1 protease activity Antimicrob","volume":"45","author":"Lindsten","year":"2001","journal-title":"Agents. Chemother."},{"key":"10.1016\/j.jbc.2024.108079_bib83","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.jcv.2006.01.014","article-title":"Non-infectious fluorimetric assay for phenotyping of drug-resistant HIV proteinase mutants","volume":"36","author":"Majerov\u00e1-Uhl\u00edkov\u00e1","year":"2006","journal-title":"J.\u00a0Clin. Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib84","doi-asserted-by":"crossref","first-page":"28134","DOI":"10.1074\/jbc.M109.095851","article-title":"Maturation mechanism of severe acute respiratory syndrome (SARS) coronavirus 3C-like proteinase","volume":"285","author":"Li","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib85","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.02559-21","article-title":"BRET-based self-cleaving biosensors for SARS-CoV-2 3CLpro inhibitor discovery","volume":"10","author":"Hou","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.jbc.2024.108079_bib86","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1038\/s41422-020-0356-z","article-title":"Boceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main protease","volume":"30","author":"Ma","year":"2020","journal-title":"Cell Res"},{"key":"10.1016\/j.jbc.2024.108079_bib87","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1126\/science.abb4489","article-title":"Structure-based design of antiviral drug candidates targeting the SARS-CoV-2 main protease","volume":"368","author":"Dai","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.108079_bib88","doi-asserted-by":"crossref","first-page":"2984","DOI":"10.1093\/jac\/dkac257","article-title":"Efficacy of ensitrelvir against SARS-CoV-2 in a delayed-treatment mouse model","volume":"77","author":"Nobori","year":"2022","journal-title":"J.\u00a0Antimicrob. Chemother."},{"key":"10.1016\/j.jbc.2024.108079_bib89","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1126\/science.abl4784","article-title":"An oral SARS-CoV-2 M(pro) inhibitor clinical candidate for the treatment of COVID-19","volume":"374","author":"Owen","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.jbc.2024.108079_bib90","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1038\/s41467-021-20900-6","article-title":"A\u00a0small molecule compound with an indole moiety inhibits the main protease of SARS-CoV-2 and blocks virus replication","volume":"12","author":"Hattori","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2024.108079_bib91","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1016\/j.chembiol.2017.09.007","article-title":"General and modular strategy for designing potent, selective, and pharmacologically compliant inhibitors of rhomboid proteases","volume":"24","author":"Tich\u00e1","year":"2017","journal-title":"Cell Chem Biol"},{"key":"10.1016\/j.jbc.2024.108079_bib92","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2116097119","article-title":"Massively parallel, computationally guided design of a proenzyme","volume":"119","author":"Yachnin","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"key":"10.1016\/j.jbc.2024.108079_bib93","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/s13238-010-0044-8","article-title":"Three-dimensional domain swapping as a mechanism to lock the active conformation in a super-active octamer of SARS-CoV main protease","volume":"1","author":"Zhang","year":"2010","journal-title":"Protein Cell"},{"key":"10.1016\/j.jbc.2024.108079_bib94","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.3390\/v16081218","article-title":"Assay development and validation for innovative antiviral development targeting the N-terminal autoprocessing of SARS-CoV-2 main protease precursors","volume":"16","author":"Huang","year":"2024","journal-title":"Viruses"},{"key":"10.1016\/j.jbc.2024.108079_bib95","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.77433","article-title":"Comprehensive fitness landscape of SARS-CoV-2 M(pro) reveals insights into viral resistance mechanisms","volume":"11","author":"Flynn","year":"2022","journal-title":"Elife"},{"key":"10.1016\/j.jbc.2024.108079_bib96","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.biochi.2019.05.004","article-title":"Exploiting the unique features of Zika and Dengue proteases for inhibitor design","volume":"166","author":"Majerov\u00e1","year":"2019","journal-title":"Biochimie"},{"key":"10.1016\/j.jbc.2024.108079_bib97","doi-asserted-by":"crossref","first-page":"14702","DOI":"10.1021\/acs.jmedchem.1c01214","article-title":"Indole chloropyridinyl ester-derived SARS-CoV-2 3CLpro inhibitors: enzyme inhibition, antiviral efficacy, structure-activity relationship, and X-ray structural studies","volume":"64","author":"Ghosh","year":"2021","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib98","doi-asserted-by":"crossref","DOI":"10.1128\/aac.00840-23","article-title":"Evaluation of in\u00a0vitro antiviral activity of SARS-CoV-2 M(pro) inhibitor pomotrelvir and cross-resistance to nirmatrelvir resistance substitutions Antimicrob","volume":"67","author":"Tong","year":"2023","journal-title":"Agents Chemother."},{"key":"10.1016\/j.jbc.2024.108079_bib99","doi-asserted-by":"crossref","first-page":"2905","DOI":"10.1021\/acs.jmedchem.1c00616","article-title":"Peptidomimetic \u03b1-acyloxymethylketone warheads with six-membered lactam P1 glutamine mimic: SARS-CoV-2 3CL protease inhibition, coronavirus antiviral activity, and in\u00a0vitro biological stability","volume":"65","author":"Bai","year":"2022","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib100","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1021\/acs.jmedchem.3c02248","article-title":"Invention of MK-7845, a SARS-CoV-2 3CL protease inhibitor employing a novel difluorinated glutamine mimic","volume":"67","author":"Shurtleff","year":"2024","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib101","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00049-24","article-title":"Optimization of quenched fluorescent peptide substrates of SARS-CoV-2 3CL(pro) main protease (Mpro) from proteomic identification of P6-P6' active site specificity","volume":"98","author":"Cesar Ramos de Jesus","year":"2024","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2024.108079_bib102","doi-asserted-by":"crossref","first-page":"27603","DOI":"10.1039\/D1RA05597F","article-title":"tert-Butyloxycarbonyl-protected amino acid ionic liquids and their application to dipeptide synthesis","volume":"11","author":"Chen","year":"2021","journal-title":"RSC Adv."},{"key":"10.1016\/j.jbc.2024.108079_bib103","doi-asserted-by":"crossref","first-page":"4282","DOI":"10.1038\/s41467-020-18096-2","article-title":"Feline coronavirus drug inhibits the main protease of SARS-CoV-2 and blocks virus replication","volume":"11","author":"Vuong","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2024.108079_bib104","doi-asserted-by":"crossref","first-page":"6807","DOI":"10.1016\/S0040-4039(01)01416-2","article-title":"An efficient synthesis of a key intermediate for the preparation of the rhinovirus protease inhibitor AG7088 via asymmetric dianionic cyanomethylation of N-Boc-l-(+)-glutamic acid dimethyl ester","volume":"42","author":"Tian","year":"2001","journal-title":"Tetrahedron Lett."},{"key":"10.1016\/j.jbc.2024.108079_bib105","doi-asserted-by":"crossref","first-page":"6767","DOI":"10.1021\/jm050548m","article-title":"Design and synthesis of peptidomimetic severe acute respiratory syndrome chymotrypsin-like protease inhibitors","volume":"48","author":"Ghosh","year":"2005","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib106","first-page":"663","article-title":"An efficient method for preparation of optically active N-protected \u03b1-amino aldehydes from N-protected \u03b1-amino alcohols collect Czech","volume":"60","author":"Sou\u010dek Milan","year":"1995","journal-title":"Chem. Commun."},{"key":"10.1016\/j.jbc.2024.108079_bib107","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1021\/ol0061485","article-title":"New\u00a0synthetic technology for efficient construction of alpha-hydroxy-beta-amino amides via the Passerini reaction","volume":"2","author":"Semple","year":"2000","journal-title":"Org. Lett."},{"key":"10.1016\/j.jbc.2024.108079_bib108","doi-asserted-by":"crossref","first-page":"110","DOI":"10.3390\/mps6060110","article-title":"Protocol for facile synthesis of fmoc-N-Me-AA-OH using 2-CTC resin as temporary and reusable protecting group","volume":"6","author":"Rom\u00e1n","year":"2023","journal-title":"Methods Protoc"},{"key":"10.1016\/j.jbc.2024.108079_bib109","first-page":"1410","article-title":"Designed parasite-selective rhomboid inhibitors block invasion and clear blood-stage malaria cell","volume":"27","author":"Gandhi","year":"2020","journal-title":"Chem. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib110","article-title":"The S1'-S3' pocket of the SARS-CoV-2 main protease is critical for substrate selectivity and can Be targeted with covalent inhibitors","volume":"62","author":"Liu","year":"2023","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.jbc.2024.108079_bib111","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1080\/14756366.2021.1901088","article-title":"Inhibition of bacterial and human zinc-metalloproteases by bisphosphonate- and catechol-containing compounds","volume":"36","author":"Rahman","year":"2021","journal-title":"J.\u00a0Enzyme Inhib. Med. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib112","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1002\/anie.197805221","article-title":"Simple method for the esterification of carboxylic acids","volume":"17","author":"Neises","year":"1978","journal-title":"Angew. Chem. Int. Edition English"},{"key":"10.1016\/j.jbc.2024.108079_bib113","doi-asserted-by":"crossref","first-page":"7119","DOI":"10.1016\/S0040-4039(97)01635-3","article-title":"A\u00a0simple linker for the attachment of aldehydes to the solid phase. Appl. Solid phase Synth. by multipin\u2122 method","volume":"38","author":"Ede","year":"1997","journal-title":"Tetrahedron Lett."},{"key":"10.1016\/j.jbc.2024.108079_bib114","doi-asserted-by":"crossref","first-page":"4395","DOI":"10.1002\/1521-3773(20011203)40:23<4395::AID-ANIE4395>3.0.CO;2-R","article-title":"Oxidizing polymers: a polymer-supported, recyclable hypervalent iodine(V) reagent for the efficient conversion of alcohols, carbonyl compounds, and unsaturated carbamates in solution","volume":"40","author":"Sorg","year":"2001","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.jbc.2024.108079_bib115","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/s41589-020-00689-z","article-title":"SARS-CoV-2 M(pro) inhibitors and activity-based probes for patient-sample imaging","volume":"17","author":"Rut","year":"2021","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib116","doi-asserted-by":"crossref","first-page":"2806","DOI":"10.1021\/jm980068d","article-title":"Structure-based design, synthesis, and biological evaluation of irreversible human rhinovirus 3C protease inhibitors. 1. Michael acceptor structure-activity studies","volume":"41","author":"Dragovich","year":"1998","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib117","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2024.107675","article-title":"Characterization of alternate encounter assemblies of SARS-CoV-2 main protease","volume":"300","author":"Aniana","year":"2024","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbc.2024.108079_bib118","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1038\/s41467-023-37035-5","article-title":"An in-solution snapshot of SARS-COV-2 main protease maturation process and inhibition","volume":"14","author":"Noske","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2024.108079_bib119","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2022.167876","article-title":"Unmasking the conformational stability and inhibitor binding to SARS-CoV-2 main protease active site mutants and miniprecursor","volume":"434","author":"Kovalevsky","year":"2022","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.jbc.2024.108079_bib120","doi-asserted-by":"crossref","first-page":"11592","DOI":"10.1021\/bi9016928","article-title":"Hepatitis C virus NS3 protease is activated by low concentrations of protease inhibitors","volume":"48","author":"Dahl","year":"2009","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbc.2024.108079_bib121","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/nmeth.1318","article-title":"Enzymatic assembly of DNA molecules up to several hundred kilobases","volume":"6","author":"Gibson","year":"2009","journal-title":"Nat. Methods."},{"key":"10.1016\/j.jbc.2024.108079_bib122","doi-asserted-by":"crossref","first-page":"2356","DOI":"10.1002\/anie.201508642","article-title":"iBodies: Modular synthetic antibody Mimetics based on hydrophilic polymers decorated with functional moieties","volume":"55","author":"\u0160\u00e1cha","year":"2016","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.jbc.2024.108079_bib123","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.1364\/OE.11.003583","article-title":"Fast calculation of fluorescence correlation data with asynchronous time-correlated single-photon counting","volume":"11","author":"Wahl","year":"2003","journal-title":"Opt. Express"},{"key":"10.1016\/j.jbc.2024.108079_bib124","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1016\/j.bpj.2020.03.011","article-title":"Membrane protein dimerization in cell-derived lipid membranes measured by FRET with MC simulations","volume":"118","author":"\u0160kerle","year":"2020","journal-title":"Biophys. J."},{"key":"10.1016\/j.jbc.2024.108079_bib125","first-page":"201","article-title":"The behavior and significance of slow-binding enzyme inhibitors","volume":"61","author":"Morrison","year":"1988","journal-title":"Adv. Enzymol. Relat. Areas Mol. Biol."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002192582402581X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002192582402581X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T21:17:17Z","timestamp":1738012637000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002192582402581X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":125,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["S002192582402581X"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2024.108079","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The zymogenic form of SARS-CoV-2 main protease: A discrete target for drug discovery","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2024.108079","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"108079"}}