{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T03:43:30Z","timestamp":1782186210543,"version":"3.54.5"},"reference-count":61,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"NIH National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM135189"],"award-info":[{"award-number":["R01GM135189"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014185","name":"Searle Scholars Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["RP170644"],"award-info":[{"award-number":["RP170644"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["RP220582"],"award-info":[{"award-number":["RP220582"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["RR150033"],"award-info":[{"award-number":["RR150033"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["S10 OD026758-01"],"award-info":[{"award-number":["S10 OD026758-01"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007914","name":"The University of Texas Southwestern Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007914","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000888","name":"W M Keck Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000888","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"National Institutes of Health Office of the Director","doi-asserted-by":"publisher","award":["S10 OD026758-01"],"award-info":[{"award-number":["S10 OD026758-01"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["AI158124"],"award-info":[{"award-number":["AI158124"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["I-1911"],"award-info":[{"award-number":["I-1911"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["I-1612"],"award-info":[{"award-number":["I-1612"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2025,4]]},"DOI":"10.1016\/j.jbc.2025.108378","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T19:07:49Z","timestamp":1741115269000},"page":"108378","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Covalent inhibition of the SARS-CoV-2 NiRAN domain via an active-site cysteine"],"prefix":"10.1016","volume":"301","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3666-8769","authenticated-orcid":false,"given":"Genaro","family":"Hernandez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0010-0733","authenticated-orcid":false,"given":"Adam","family":"Osinski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3516-5855","authenticated-orcid":false,"given":"Abir","family":"Majumdar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Eitson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Antczak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Paw\u0142owski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanspeter","family":"Niederstrasser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7183-2865","authenticated-orcid":false,"given":"Kelly A.","family":"Servage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce","family":"Posner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Schoggins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph M.","family":"Ready","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent S.","family":"Tagliabracci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2025.108378_bib1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s13643-024-02476-6","article-title":"The global economic burden of COVID-19 disease: a comprehensive systematic review and meta-analysis","volume":"13","author":"Faramarzi","year":"2024","journal-title":"Syst. Rev."},{"key":"10.1016\/j.jbc.2025.108378_bib2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.3389\/fpubh.2020.00241","article-title":"Economic consequences of the COVID-19 outbreak: the need for epidemic preparedness","volume":"8","author":"Pak","year":"2020","journal-title":"Front. Public Health"},{"key":"10.1016\/j.jbc.2025.108378_bib3","doi-asserted-by":"crossref","DOI":"10.1017\/S0266462321000155","article-title":"The broader societal impacts of COVID-19 and the growing importance of capturing these in health economic analyses","volume":"37","author":"Schnitzler","year":"2021","journal-title":"Int. J. Technol. Assess. Health Care"},{"key":"10.1016\/j.jbc.2025.108378_bib4","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41586-020-2012-7","article-title":"A\u00a0pneumonia outbreak associated with a new coronavirus of probable bat origin","volume":"579","author":"Zhou","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2025.108378_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.742167","article-title":"Implication of SARS-CoV-2 immune escape spike variants on secondary and vaccine breakthrough infections","volume":"12","author":"Ahmad","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jbc.2025.108378_bib6","article-title":"SARS-CoV-2 vaccine breakthrough infections of omicron and delta variants in healthcare workers","volume":"11","author":"Regenhardt","year":"2023","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.jbc.2025.108378_bib7","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/j.cell.2022.03.019","article-title":"Neutralizing immunity in vaccine breakthrough infections from the SARS-CoV-2 Omicron and Delta variants","volume":"185","author":"Servellita","year":"2022","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2025.108378_bib8","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1056\/NEJMra2106441","article-title":"The vaccine-hesitant moment","volume":"387","author":"Larson","year":"2022","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.jbc.2025.108378_bib9","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1056\/NEJMoa2007764","article-title":"Remdesivir for the treatment of covid-19 - final report","volume":"383","author":"Beigel","year":"2020","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.jbc.2025.108378_bib10","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1056\/NEJMoa2118542","article-title":"Oral nirmatrelvir for high-risk, Nonhospitalized adults with covid-19","volume":"386","author":"Hammond","year":"2022","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.jbc.2025.108378_bib11","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1056\/NEJMoa2116044","article-title":"Molnupiravir for oral treatment of covid-19 in Nonhospitalized patients","volume":"386","author":"Jayk Bernal","year":"2022","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.jbc.2025.108378_bib12","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1126\/science.abl4784","article-title":"An oral SARS-CoV-2 M(pro) inhibitor clinical candidate for the treatment of COVID-19","volume":"374","author":"Owen","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.jbc.2025.108378_bib13","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1016\/S0140-6736(20)31022-9","article-title":"Remdesivir in adults with severe COVID-19: a randomised, double-blind, placebo-controlled, multicentre trial","volume":"395","author":"Wang","year":"2020","journal-title":"Lancet"},{"key":"10.1016\/j.jbc.2025.108378_bib14","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1056\/NEJMoa2116846","article-title":"Early remdesivir to prevent progression to severe covid-19 in outpatients","volume":"386","author":"Gottlieb","year":"2022","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.jbc.2025.108378_bib15","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1021\/acscentsci.3c00538","article-title":"Naturally occurring mutations of SARS-CoV-2 main protease confer drug resistance to nirmatrelvir","volume":"9","author":"Hu","year":"2023","journal-title":"ACS Cent. Sci."},{"key":"10.1016\/j.jbc.2025.108378_bib16","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/j.chembiol.2024.03.008","article-title":"SARS-CoV-2 resistance to monoclonal antibodies and small-molecule drugs","volume":"31","author":"Iketani","year":"2024","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.jbc.2025.108378_bib17","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/s41586-022-05514-2","article-title":"Multiple pathways for SARS-CoV-2 resistance to nirmatrelvir","volume":"613","author":"Iketani","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2025.108378_bib18","doi-asserted-by":"crossref","first-page":"12500","DOI":"10.1021\/acs.jmedchem.2c01005","article-title":"Targeting SARS-CoV-2 main protease for treatment of COVID-19: covalent inhibitors structure-activity relationship insights and evolution perspectives","volume":"65","author":"La Monica","year":"2022","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2025.108378_bib19","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.02428-20","article-title":"Human safety, tolerability, and pharmacokinetics of molnupiravir, a novel broad-spectrum oral antiviral agent with activity against SARS-CoV-2 antimicrob","volume":"65","author":"Painter","year":"2021","journal-title":"Agents Chemother."},{"key":"10.1016\/j.jbc.2025.108378_bib20","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1007\/s43440-022-00388-7","article-title":"Oral antiviral treatments for COVID-19: opportunities and challenges","volume":"74","author":"Rahmah","year":"2022","journal-title":"Pharmacol. Rep."},{"key":"10.1016\/j.jbc.2025.108378_bib21","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1128\/jvi.41.2.557-565.1982","article-title":"Further characterization of mRNA's of mouse hepatitis virus: presence of common 5'-end nucleotides","volume":"41","author":"Lai","year":"1982","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.jbc.2025.108378_bib22","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s12250-016-3726-4","article-title":"Molecular mechanisms of coronavirus RNA capping and methylation","volume":"31","author":"Chen","year":"2016","journal-title":"Virol. Sin."},{"key":"10.1016\/j.jbc.2025.108378_bib23","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/s41586-022-05185-z","article-title":"The mechanism of RNA capping by SARS-CoV-2","volume":"609","author":"Park","year":"2022","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2025.108378_bib24","doi-asserted-by":"crossref","first-page":"4347","DOI":"10.1016\/j.cell.2022.09.037","article-title":"A\u00a0mechanism for SARS-CoV-2 RNA capping and its inhibition by nucleotide analog inhibitors","volume":"185","author":"Yan","year":"2022","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2025.108378_bib25","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2022310118","article-title":"Coronavirus replication-transcription complex: vital and selective NMPylation of a conserved site in nsp9 by the NiRAN-RdRp subunit","volume":"118","author":"Slanina","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2025.108378_bib26","doi-asserted-by":"crossref","first-page":"8822","DOI":"10.1093\/nar\/gkab677","article-title":"NMPylation and de-NMPylation of SARS-CoV-2 nsp9 by the NiRAN domain","volume":"49","author":"Wang","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jbc.2025.108378_bib27","doi-asserted-by":"crossref","first-page":"3921","DOI":"10.1016\/j.molcel.2023.10.001","article-title":"Structural and functional insights into the enzymatic plasticity of the SARS-CoV-2 NiRAN Domain","volume":"83","author":"Small","year":"2023","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jbc.2025.108378_bib28","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A\u00a0simple statistical parameter for use in evaluation and validation of high throughput screening assays","volume":"4","author":"Zhang","year":"1999","journal-title":"J.\u00a0Biomol. Screen"},{"key":"10.1016\/j.jbc.2025.108378_bib29","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1177\/1087057107312628","article-title":"Quantitative assessment of hit detection and confirmation in single and duplicate high-throughput screenings","volume":"13","author":"Wu","year":"2008","journal-title":"J.\u00a0Biomol. Screen"},{"key":"10.1016\/j.jbc.2025.108378_bib30","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1021\/jm901137j","article-title":"New substructure filters for removal of pan assay interference compounds (PAINS) from screening libraries and for their exclusion in bioassays","volume":"53","author":"Baell","year":"2010","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.jbc.2025.108378_bib31","doi-asserted-by":"crossref","DOI":"10.3390\/molecules27227728","article-title":"Covalent warheads targeting cysteine residue: promising approach drug development","volume":"27","author":"Huang","year":"2022","journal-title":"Molecules"},{"key":"10.1016\/j.jbc.2025.108378_bib32","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.cell.2018.08.046","article-title":"Protein AMPylation by an evolutionarily conserved Pseudokinase","volume":"175","author":"Sreelatha","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2025.108378_bib33","doi-asserted-by":"crossref","first-page":"2617","DOI":"10.1039\/C9CS00720B","article-title":"Advances in covalent kinase inhibitors","volume":"49","author":"Abdeldayem","year":"2020","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.jbc.2025.108378_bib34","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1038\/s41586-022-05664-3","article-title":"Structural basis for substrate selection by the SARS-CoV-2 replicase","volume":"614","author":"Malone","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2025.108378_bib35","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/s41467-022-28113-1","article-title":"A\u00a0dual mechanism of action of AT-527 against SARS-CoV-2 polymerase","volume":"13","author":"Shannon","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2025.108378_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.coviro.2022.101279","article-title":"Nucleoside analogs for management of respiratory virus infections: mechanism of action and clinical efficacy","volume":"57","author":"Stevaert","year":"2022","journal-title":"Curr. Opin. Virol."},{"key":"10.1016\/j.jbc.2025.108378_bib37","doi-asserted-by":"crossref","first-page":"8416","DOI":"10.1093\/nar\/gkv838","article-title":"Discovery of an essential nucleotidylating activity associated with a newly delineated conserved domain in the RNA polymerase-containing protein of all nidoviruses","volume":"43","author":"Lehmann","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jbc.2025.108378_bib38","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/s41586-020-2286-9","article-title":"A\u00a0SARS-CoV-2 protein interaction map reveals targets for drug repurposing","volume":"583","author":"Gordon","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2025.108378_bib39","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1021\/acsinfecdis.9b00012","article-title":"Quantifying the interactions between biomolecules: guidelines for assay design and data analysis","volume":"5","author":"Tonge","year":"2019","journal-title":"ACS Infect. Dis."},{"key":"10.1016\/j.jbc.2025.108378_bib40","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1101\/gr.849004","article-title":"WebLogo: a sequence logo generator","volume":"14","author":"Crooks","year":"2004","journal-title":"Genome Res."},{"key":"10.1016\/j.jbc.2025.108378_bib41","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1038\/nbt.3988","article-title":"MMseqs2 enables sensitive protein sequence searching for the analysis of massive data sets","volume":"35","author":"Steinegger","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jbc.2025.108378_bib42","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1093\/bib\/bbx108","article-title":"MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization","volume":"20","author":"Katoh","year":"2019","journal-title":"Brief Bioinform."},{"key":"10.1016\/j.jbc.2025.108378_bib43","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/s41586-020-2368-8","article-title":"Structure of replicating SARS-CoV-2 polymerase","volume":"584","author":"Hillen","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2025.108378_bib44","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.chom.2020.12.009","article-title":"Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors","volume":"29","author":"Hoffmann","year":"2021","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.jbc.2025.108378_bib45","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1038\/s41564-023-01431-w","article-title":"LY6E is a pan-coronavirus restriction factor in the respiratory tract","volume":"8","author":"Mar","year":"2023","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.jbc.2025.108378_bib46","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3001091","article-title":"A\u00a0plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research","volume":"19","author":"Rihn","year":"2021","journal-title":"Plos Biol."},{"key":"10.1016\/j.jbc.2025.108378_bib47","series-title":"Chiral macrocyclic compounds as protein kinase inhibitors and uses thereof. CN114763360A\u00b72022-07-19","author":"SHUGUAN","year":"2022"},{"key":"10.1016\/j.jbc.2025.108378_bib48","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jsb.2005.07.007","article-title":"Automated electron microscope tomography using robust prediction of specimen movements","volume":"152","author":"Mastronarde","year":"2005","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.jbc.2025.108378_bib49","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.jsb.2012.09.006","article-title":"RELION: implementation of a Bayesian approach to cryo-EM structure determination","volume":"180","author":"Scheres","year":"2012","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.jbc.2025.108378_bib50","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nmeth.4193","article-title":"MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy","volume":"14","author":"Zheng","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jbc.2025.108378_bib51","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/s42003-019-0437-z","article-title":"SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM","volume":"2","author":"Wagner","year":"2019","journal-title":"Commun. Biol."},{"key":"10.1016\/j.jbc.2025.108378_bib52","series-title":"asarnow\/pyem: UCSF pyem v0.5 (v0.5)","author":"Asarnow","year":"2019"},{"key":"10.1016\/j.jbc.2025.108378_bib53","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera--a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.jbc.2025.108378_bib54","doi-asserted-by":"crossref","first-page":"e4792","DOI":"10.1002\/pro.4792","article-title":"UCSF ChimeraX: tools for structure building and analysis","volume":"32","author":"Meng","year":"2023","journal-title":"Protein Sci."},{"key":"10.1016\/j.jbc.2025.108378_bib55","series-title":"The PyMOL Molecular Graphics System","author":"Schr\u00f6dinger","year":"2015"},{"key":"10.1016\/j.jbc.2025.108378_bib56","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2025.108378_bib57","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1107\/S2059798318002425","article-title":"ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps","volume":"74","author":"Croll","year":"2018","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"10.1016\/j.jbc.2025.108378_bib58","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1107\/S2059798317000067","article-title":"AceDRG: a stereochemical description generator for ligands","volume":"73","author":"Long","year":"2017","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"10.1016\/j.jbc.2025.108378_bib59","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta crystallographica Sect. D, Biol. Crystallogr."},{"key":"10.1016\/j.jbc.2025.108378_bib60","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/pro.3330","article-title":"MolProbity: more and better reference data for improved all-atom structure validation","volume":"27","author":"Williams","year":"2018","journal-title":"Protein Sci."},{"key":"10.1016\/j.jbc.2025.108378_bib61","doi-asserted-by":"crossref","first-page":"2778","DOI":"10.1021\/ci200227u","article-title":"LigPlot+: multiple ligand-protein interaction diagrams for drug discovery","volume":"51","author":"Laskowski","year":"2011","journal-title":"J.\u00a0Chem. Inf. Model."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925825002273?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925825002273?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T22:59:32Z","timestamp":1778626772000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925825002273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":61,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["S0021925825002273"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2025.108378","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.11.22.624893","asserted-by":"object"}]},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Covalent inhibition of the SARS-CoV-2 NiRAN domain via an active-site cysteine","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2025.108378","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology\u00e9","name":"copyright","label":"Copyright"}],"article-number":"108378"}}