{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:49:13Z","timestamp":1787921353382,"version":"build-2784847793"},"reference-count":83,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32 GM158458"],"award-info":[{"award-number":["T32 GM158458"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35 GM133497"],"award-info":[{"award-number":["R35 GM133497"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P30GM133894"],"award-info":[{"award-number":["P30GM133894"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["GT15765"],"award-info":[{"award-number":["GT15765"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000937","name":"M J Murdock Charitable Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000937","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006151","name":"Basic Energy Sciences","doi-asserted-by":"publisher","award":["76SF00515"],"award-info":[{"award-number":["76SF00515"]}],"id":[{"id":"10.13039\/100006151","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006151","name":"Basic Energy Sciences","doi-asserted-by":"publisher","award":["DE-AC02"],"award-info":[{"award-number":["DE-AC02"]}],"id":[{"id":"10.13039\/100006151","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000015","name":"U.S. Department of Energy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006132","name":"Office of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006132","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006206","name":"Biological and Environmental Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006206","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016549","name":"Universal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016549","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.jbc.2025.110801","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T23:01:03Z","timestamp":1762383663000},"page":"110801","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Pseudomonas aeruginosa membrane histidine kinase BqsS\/CarS directly senses environmental ferrous iron (Fe2+)"],"prefix":"10.1016","volume":"301","author":[{"given":"Alexander","family":"Paredes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chioma","family":"Iheacho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly N.","family":"Chac\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9332-8683","authenticated-orcid":false,"given":"Aaron T.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2025.110801_bib1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.jhin.2009.04.020","article-title":"Pseudomonas aeruginosa: a formidable and ever-present adversary","volume":"73","author":"Kerr","year":"2009","journal-title":"J. Hosp. Infect."},{"key":"10.1016\/j.jbc.2025.110801_bib2","doi-asserted-by":"crossref","first-page":"8671","DOI":"10.3390\/ijms21228671","article-title":"Pseudomonas Aeruginosa biofilms","volume":"21","author":"Thi","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbc.2025.110801_bib3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0005513","article-title":"Pseudomonas Aeruginosa PAO1 preferentially grows as aggregates in liquid batch cultures and disperses upon starvation","volume":"4","author":"Schleheck","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.jbc.2025.110801_bib4","doi-asserted-by":"crossref","first-page":"6746","DOI":"10.1128\/JB.183.23.6746-6751.2001","article-title":"Biofilms and planktonic cells of Pseudomonas Aeruginosa have similar resistance to killing by antimicrobials","volume":"183","author":"Spoering","year":"2001","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbc.2025.110801_bib5","first-page":"43","article-title":"Microbes and environment","author":"Gupta","year":"2016","journal-title":"Princ. Appl. Environ. Biotechnol. Sustain. Future"},{"key":"10.1016\/j.jbc.2025.110801_bib6","doi-asserted-by":"crossref","DOI":"10.1099\/mgen.0.000370","article-title":"Antibiotic resistance in Pseudomonas Aeruginosa and adaptation to complex dynamic environments","volume":"6","author":"Sommer","year":"2020","journal-title":"Microb. Genomics"},{"key":"10.1016\/j.jbc.2025.110801_bib7","doi-asserted-by":"crossref","first-page":"913","DOI":"10.3389\/fmicb.2019.00913","article-title":"Tolerance and resistance of Pseudomonas Aeruginosa biofilms to antimicrobial agents-how P. Aeruginosa can escape antibiotics","volume":"10","author":"Ciofu","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jbc.2025.110801_bib8","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s13756-019-0533-3","article-title":"Antibiotics versus biofilm: an emerging battleground in microbial communities","volume":"8","author":"Sharma","year":"2019","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"10.1016\/j.jbc.2025.110801_bib9","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1021\/acs.biochem.3c00296","article-title":"Metal messengers: communication in the bacterial world through transition-metal-sensing two-component systems","volume":"62","author":"Paredes","year":"2023","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbc.2025.110801_bib10","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/nrmicro2836","article-title":"Nutritional immunity: transition metals at the pathogen\u2013host interface","volume":"10","author":"Hood","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jbc.2025.110801_bib11","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.VMBF-0027-2015","article-title":"Bacterial metabolism shapes the host\u2013pathogen interface","volume":"4","author":"Passalacqua","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.jbc.2025.110801_bib12","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.chom.2018.05.008","article-title":"The impact of dietary transition metals on host-bacterial interactions","volume":"23","author":"Lopez","year":"2018","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.jbc.2025.110801_bib13","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1146\/annurev-genet-120215-035146","article-title":"Transition metals and virulence in bacteria","volume":"50","author":"Palmer","year":"2016","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/j.jbc.2025.110801_bib14","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.cbpa.2016.09.012","article-title":"Metal homeostasis in infectious disease: recent advances in bacterial metallophores and the human metal-withholding response","volume":"37","author":"Neumann","year":"2017","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.jbc.2025.110801_bib15","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1042\/EBC20160084","article-title":"Transition metals at the host\u2013pathogen interface: how Neisseria exploit human metalloproteins for acquiring iron and zinc","volume":"61","author":"Neumann","year":"2017","journal-title":"Essays Biochem."},{"key":"10.1016\/j.jbc.2025.110801_bib16","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1146\/annurev-biochem-062917-012312","article-title":"Transition metal sequestration by the host-defense protein Calprotectin","volume":"87","author":"Zygiel","year":"2018","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.jbc.2025.110801_bib17","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1146\/annurev.ge.26.120192.000443","article-title":"Communication modules in bacterial signaling proteins","volume":"26","author":"Parkinson","year":"1992","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/j.jbc.2025.110801_bib18","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1104\/pp.117.3.723","article-title":"The two-component System1: regulation of diverse signaling pathways in prokaryotes and eukaryotes","volume":"117","author":"Chang","year":"1998","journal-title":"Plant Physiol."},{"key":"10.1016\/j.jbc.2025.110801_bib19","doi-asserted-by":"crossref","first-page":"7850","DOI":"10.1073\/pnas.83.20.7850","article-title":"Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC","volume":"83","author":"Nixon","year":"1986","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2025.110801_bib20","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.tibs.2007.03.002","article-title":"Bacterial response regulators: versatile regulatory strategies from common domains","volume":"32","author":"Gao","year":"2007","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.jbc.2025.110801_bib21","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1146\/annurev.biochem.69.1.183","article-title":"Two-component signal transduction","volume":"69","author":"Stock","year":"2000","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.jbc.2025.110801_bib22","series-title":"Streptococcus Pyogenes: Basic Biology to Clinical Manifestations","article-title":"Virulence-related transcriptional regulators of Streptococcus Pyogenes","author":"Vega","year":"2022"},{"key":"10.1016\/j.jbc.2025.110801_bib23","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1080\/21505594.2022.2127196","article-title":"Two-component systems regulate bacterial virulence in response to the host gastrointestinal environment and metabolic cues","volume":"13","author":"Shaw","year":"2022","journal-title":"Virulence"},{"key":"10.1016\/j.jbc.2025.110801_bib24","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1042\/BST20180546","article-title":"Control Freaks\u2014signals and cues governing the regulation of virulence in attaching and effacing pathogens","volume":"47","author":"Turner","year":"2018","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.jbc.2025.110801_bib25","article-title":"The response regulator OmpR contributes to the pathogenicity of Avian pathogenic Escherichia Coli","volume":"101","author":"Fu","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.jbc.2025.110801_bib26","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.tim.2024.07.009","article-title":"Temperature sensing and virulence regulation in pathogenic bacteria","volume":"33","author":"Roncarati","year":"2025","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.jbc.2025.110801_bib27","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a012427","article-title":"Bacterial quorum sensing: its role in virulence and possibilities for its control","volume":"2","author":"Rutherford","year":"2012","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.jbc.2025.110801_bib28","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1128\/JB.02252-12","article-title":"The interacting cra and KdpE regulators are involved in the expression of multiple virulence factors in enterohemorrhagic Escherichia Coli","volume":"195","author":"Njoroge","year":"2013","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbc.2025.110801_bib29","doi-asserted-by":"crossref","first-page":"88","DOI":"10.4161\/cib.1.1.6717","article-title":"A novel two-component system BqsS-BqsR modulates quorum sensing-dependent biofilm decay in Pseudomonas Aeruginosa","volume":"1","author":"Dong","year":"2008","journal-title":"Commun. Integr. Biol."},{"key":"10.1016\/j.jbc.2025.110801_bib30","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1128\/JB.05634-11","article-title":"BqsR\/BqsS constitute a two-component system that senses extracellular Fe(II) in Pseudomonas Aeruginosa","volume":"194","author":"Kreamer","year":"2012","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbc.2025.110801_bib31","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1128\/JB.00963-15","article-title":"The Pseudomonas Aeruginosa PAO1 two-component regulator CarSR regulates calcium homeostasis and calcium-induced virulence factor production through its regulatory targets CarO and CarP","volume":"198","author":"Guragain","year":"2016","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbc.2025.110801_bib32","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1046\/j.1365-2222.2000.00988.x","article-title":"The relationship of eosinophil granule proteins to ions in the sputum of patients with cystic fibrosis","volume":"30","author":"Halmerbauer","year":"2000","journal-title":"Clin. Exp. Allergy J. Br. Soc. Allergy Clin. Immunol."},{"key":"10.1016\/j.jbc.2025.110801_bib33","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1136\/adc.48.4.267","article-title":"Flow rate and inorganic components of submandibular saliva in cystic fibrosis","volume":"48","author":"Blomfield","year":"1973","journal-title":"Arch. Dis. Child."},{"key":"10.1016\/j.jbc.2025.110801_bib34","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1378\/chest.114.3.814","article-title":"Increased iron and ferritin content of sputum from patients with cystic Fibrosis or chronic bronchitis","volume":"114","author":"Stites","year":"1998","journal-title":"Chest"},{"key":"10.1016\/j.jbc.2025.110801_bib35","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-67729-5","article-title":"Altered iron metabolism in cystic Fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas Aeruginosa survival","volume":"10","author":"Hazlett","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbc.2025.110801_bib36","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.jcf.2012.10.010","article-title":"Iron accumulates in the lavage and explanted lungs of cystic fibrosis patients","volume":"12","author":"Ghio","year":"2013","journal-title":"J. Cyst. Fibros."},{"key":"10.1016\/j.jbc.2025.110801_bib37","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1183\/09031936.00154006","article-title":"Increased airway iron as a potential factor in the persistence of Pseudomonas Aeruginosa infection in cystic fibrosis","volume":"30","author":"Reid","year":"2007","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.jbc.2025.110801_bib38","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00557-13","article-title":"Ferrous iron is a significant component of bioavailable iron in cystic Fibrosis airways","volume":"4","author":"Hunter","year":"2013","journal-title":"mBio"},{"key":"10.1016\/j.jbc.2025.110801_bib39","series-title":"Methods in Enzymology","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S0076-6879(09)63035-4","article-title":"Chapter 35 purification of membrane proteins","volume":"463","author":"Lin","year":"2009"},{"key":"10.1016\/j.jbc.2025.110801_bib40","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1038\/s41467-024-55671-3","article-title":"Mechanism of sensor kinase CitA transmembrane signaling","volume":"16","author":"Zhang","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2025.110801_bib41","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1016\/j.bbamem.2012.02.015","article-title":"Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism","volume":"1818","author":"Patching","year":"2012","journal-title":"Biochim. Biophys. Acta BBA Biomembr."},{"key":"10.1016\/j.jbc.2025.110801_bib42","doi-asserted-by":"crossref","first-page":"eaah6345","DOI":"10.1126\/science.aah6345","article-title":"Mechanism of transmembrane signaling by sensor histidine kinases","volume":"356","author":"Gushchin","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.jbc.2025.110801_bib43","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.jmb.2011.08.011","article-title":"Determinants of homodimerization specificity in histidine kinases","volume":"413","author":"Ashenberg","year":"2011","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jbc.2025.110801_bib44","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2002-3-10-reviews3013","article-title":"Histidine protein kinases: key signal transducers outside the animal kingdom","volume":"3","author":"Wolanin","year":"2002","journal-title":"Genome Biol."},{"key":"10.1016\/j.jbc.2025.110801_bib45","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02549-14","article-title":"The ferrous iron-responsive BqsRS two-component system activates genes that promote cationic stress tolerance","volume":"6","author":"Kreamer","year":"2015","journal-title":"mBio"},{"key":"10.1016\/j.jbc.2025.110801_bib46","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1093\/femsre\/fuv049","article-title":"Bacterial ferrous iron transport: the feo system","volume":"40","author":"Lau","year":"2016","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.jbc.2025.110801_bib47","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1126\/science.271.5255.1552","article-title":"A permease-oxidase complex involved in high-affinity iron uptake in yeast","volume":"271","author":"Stearman","year":"1996","journal-title":"Science"},{"key":"10.1016\/j.jbc.2025.110801_bib48","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1126\/science.288.5468.1062","article-title":"A high-affinity iron permease essential for Candida Albicans virulence","volume":"288","author":"Ramanan","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.jbc.2025.110801_bib49","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1006\/jmbi.1995.0274","article-title":"High resolution crystal structures of amphibian red-cell L ferritin: potential roles for structural plasticity and solvation in function","volume":"248","author":"Trikha","year":"1995","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jbc.2025.110801_bib50","doi-asserted-by":"crossref","first-page":"6162","DOI":"10.1128\/JB.01465-12","article-title":"Characterization of a ferrous iron-responsive two-component system in nontypeable Haemophilus Influenzae","volume":"194","author":"Steele","year":"2012","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbc.2025.110801_bib51","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1006133","article-title":"Two-component signaling system VgrRS directly senses extracytoplasmic and intracellular iron to control bacterial adaptation under iron depleted stress","volume":"12","author":"Wang","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jbc.2025.110801_bib52","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1080\/03079457.2020.1781791","article-title":"The two-component system, BasSR, is involved in the regulation of biofilm and virulence in Avian pathogenic Escherichia Coli","volume":"49","author":"Yu","year":"2020","journal-title":"Avian Pathol."},{"key":"10.1016\/j.jbc.2025.110801_bib53","doi-asserted-by":"crossref","first-page":"1758","DOI":"10.1271\/bbb.68.1758","article-title":"A genome-wide view of the Escherichia Coli BasS\u2013BasR two-component system implicated in iron-responses","volume":"68","author":"Hagiwara","year":"2004","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.jbc.2025.110801_bib54","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0092-8674(00)00092-1","article-title":"A signal transduction system that responds to extracellular iron","volume":"103","author":"W\u00f6sten","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2025.110801_bib55","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1107\/S0909049596005651","article-title":"State-of-the-Art analysis of whole X-Ray absorption spectra","volume":"3","author":"Binsted","year":"1996","journal-title":"J. Synchrotron Radiat."},{"key":"10.1016\/j.jbc.2025.110801_bib56","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1021\/ic00109a007","article-title":"X-Ray absorption pre-edge studies of high-spin Iron(II) complexes","volume":"34","author":"Randall","year":"1995","journal-title":"Inorg. Chem."},{"key":"10.1016\/j.jbc.2025.110801_bib57","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41579-018-0055-7","article-title":"Structural insights into the signalling mechanisms of two-component systems","volume":"16","author":"Jacob-Dubuisson","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jbc.2025.110801_bib58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pep.2017.09.007","article-title":"Expression and purification of functionally active ferrous iron transporter FeoB from Klebsiella Pneumoniae","volume":"142","author":"Smith","year":"2018","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.jbc.2025.110801_bib59","doi-asserted-by":"crossref","DOI":"10.3390\/ijms251810127","article-title":"Impact of metal ions on cellular functions: a focus on mesenchymal stem\/stromal cell differentiation","volume":"25","author":"Peters","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbc.2025.110801_bib60","doi-asserted-by":"crossref","first-page":"60","DOI":"10.2478\/intox-2014-0009","article-title":"Toxicity, mechanism and health effects of some heavy metals","volume":"7","author":"Jaishankar","year":"2014","journal-title":"Interdiscip. Toxicol."},{"key":"10.1016\/j.jbc.2025.110801_bib61","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1016\/j.ccr.2010.01.005","article-title":"Iron\u2013dinitrogen coordination chemistry: dinitrogen activation and reactivity","volume":"254","author":"Crossland","year":"2010","journal-title":"Coord. Chem. Rev."},{"key":"10.1016\/j.jbc.2025.110801_bib62","doi-asserted-by":"crossref","first-page":"3249","DOI":"10.1073\/pnas.74.8.3249","article-title":"Isolation of an iron-molybdenum cofactor from nitrogenase","volume":"74","author":"Shah","year":"1977","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2025.110801_bib63","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0168-6445(03)00055-X","article-title":"Bacterial iron homeostasis","volume":"27","author":"Andrews","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.jbc.2025.110801_bib64","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/s41579-022-00745-6","article-title":"Nutritional immunity: the battle for nutrient metals at the host\u2013pathogen interface","volume":"20","author":"Murdoch","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jbc.2025.110801_bib65","series-title":"Pseudomonas: Volume 4 Molecular Biology of Emerging Issues","first-page":"1","article-title":"New insights on iron acquisition mechanisms in pathogenic pseudomonas","author":"Schalk","year":"2006"},{"key":"10.1016\/j.jbc.2025.110801_bib66","doi-asserted-by":"crossref","first-page":"3283","DOI":"10.3390\/ijms20133283","article-title":"The impact of iron overload and ferroptosis on reproductive disorders in humans: implications for Preeclampsia","volume":"20","author":"Ng","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbc.2025.110801_bib67","doi-asserted-by":"crossref","first-page":"6418","DOI":"10.1038\/ncomms7418","article-title":"Dysregulation of transition metal ion homeostasis is the molecular basis for cadmium toxicity in Streptococcus Pneumoniae","volume":"6","author":"Begg","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2025.110801_bib68","doi-asserted-by":"crossref","first-page":"11076","DOI":"10.1073\/pnas.0504266102","article-title":"Iron and Pseudomonas Aeruginosa biofilm Formation","volume":"102","author":"Banin","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2025.110801_bib69","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1128\/JB.01473-07","article-title":"Influence of quorum sensing and iron on twitching motility and biofilm Formation in Pseudomonas Aeruginosa","volume":"190","author":"Patriquin","year":"2008","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbc.2025.110801_bib70","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/S0966-842X(00)01913-2","article-title":"Mechanisms of biofilm resistance to antimicrobial agents","volume":"9","author":"Mah","year":"2001","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.jbc.2025.110801_bib71","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1128\/CMR.15.2.167-193.2002","article-title":"Biofilms: survival mechanisms of clinically relevant microorganisms","volume":"15","author":"Donlan","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.jbc.2025.110801_bib72","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.medcli.2009.11.012","article-title":"The role of iron in the interaction between host and pathogen","volume":"134","author":"del Castillo-Rueda","year":"2010","journal-title":"Med. Clin. (Barc.)"},{"key":"10.1016\/j.jbc.2025.110801_bib73","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1128\/JB.185.3.1027-1036.2003","article-title":"Rhamnolipid surfactant production affects biofilm Architecture in Pseudomonas Aeruginosa PAO1","volume":"185","author":"Davey","year":"2003","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbc.2025.110801_bib74","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1021\/ac60289a016","article-title":"Ferrozine---a new spectrophotometric reagent for iron","volume":"42","author":"Stookey","year":"1970","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jbc.2025.110801_bib75","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.ab.2014.02.026","article-title":"Optimized ferrozine-based assay for dissolved iron","volume":"454","author":"Jeitner","year":"2014","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jbc.2025.110801_bib76","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/s41467-023-36158-z","article-title":"Iron-sulfur clusters are involved in post-translational arginylation","volume":"14","author":"Van","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2025.110801_bib77","series-title":"EXAFSPAK","author":"George","year":"1995"},{"key":"10.1016\/j.jbc.2025.110801_bib78","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1088\/0022-3719\/17\/1\/019","article-title":"A rapid, exact curved-wave theory for EXAFS calculations","volume":"17","author":"Gurman","year":"1984","journal-title":"J. Phys. C Solid State Phys."},{"key":"10.1016\/j.jbc.2025.110801_bib79","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1088\/0022-3719\/19\/11\/021","article-title":"Rapid, exact, curved-wave theory for EXAFS calculations. II. The multiple-scattering contributions","volume":"19","author":"Gurman","year":"1986","journal-title":"J. Phys. C Solid State Phys."},{"key":"10.1016\/j.jbc.2025.110801_bib80","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2025.110801_bib81","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jbc.2025.110801_bib82","first-page":"175","article-title":"A hidden markov model for predicting transmembrane helices in protein sequences","volume":"6","author":"Sonnhammer","year":"1998","journal-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol."},{"key":"10.1016\/j.jbc.2025.110801_bib83","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1093\/bioinformatics\/btt607","article-title":"Protter: interactive protein feature visualization and integration with experimental proteomic data","volume":"30","author":"Omasits","year":"2014","journal-title":"Bioinformatics"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925825026535?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925825026535?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T15:29:19Z","timestamp":1773329359000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925825026535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":83,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["S0021925825026535"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2025.110801","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The Pseudomonas aeruginosa membrane histidine kinase BqsS\/CarS directly senses environmental ferrous iron (Fe2+)","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2025.110801","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology\u00e9","name":"copyright","label":"Copyright"}],"article-number":"110801"}}