{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T16:55:16Z","timestamp":1780505716780,"version":"3.54.1"},"reference-count":43,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001664","name":"Leibnitz Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001664","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Forschung, Technologie und Raumfahrt","doi-asserted-by":"publisher","award":["13N15713"],"award-info":[{"award-number":["13N15713"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013457","name":"Center for Sepsis Control and Care","doi-asserted-by":"publisher","award":["FKZ 01EO1502"],"award-info":[{"award-number":["FKZ 01EO1502"]}],"id":[{"id":"10.13039\/501100013457","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"EU Framework Programme for Research and Innovation Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["861122"],"award-info":[{"award-number":["861122"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100024886","name":"Friedrich-Loeffler-Institut","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024886","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.jbc.2025.110940","type":"journal-article","created":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T20:57:20Z","timestamp":1763326640000},"page":"110940","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Insights into chlamydial infection at the sub-cellular level using label-free Raman spectroscopy in comparison to electron microscopy"],"prefix":"10.1016","volume":"302","author":[{"given":"Nancy","family":"Unger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0881-6052","authenticated-orcid":false,"given":"Elisabeth M.","family":"Liebler-Tenorio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8910-4137","authenticated-orcid":false,"given":"Rustam R.","family":"Guliev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Eiserloh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandor","family":"Nietzsche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frauke","family":"Nowak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Zuchantke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Berens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7643-9114","authenticated-orcid":false,"given":"Christiane","family":"Schnee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Neugebauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2025.110940_bib1","doi-asserted-by":"crossref","DOI":"10.1155\/2019\/1356540","article-title":"Intracellular pathogens: host immunity and microbial persistence strategies","volume":"2019","author":"Thakur","year":"2019","journal-title":"J. Immunol. Res."},{"key":"10.1016\/j.jbc.2025.110940_bib2","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.3390\/microorganisms8091405","article-title":"Zoonotic diseases: etiology, impact, and control","volume":"8","author":"Rahman","year":"2020","journal-title":"Microorganisms"},{"key":"10.1016\/j.jbc.2025.110940_bib3","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s40588-015-0014-2","article-title":"Chlamydia abortus: new aspects of infectious abortion in sheep and potential risk for pregnant women","volume":"2","author":"Essig","year":"2015","journal-title":"Curr. Clin. Microbiol. Rep."},{"key":"10.1016\/j.jbc.2025.110940_bib4","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1053\/jcpa.2002.0629","article-title":"Animal chlamydioses and zoonotic implications","volume":"128","author":"Longbottom","year":"2003","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.jbc.2025.110940_bib5","doi-asserted-by":"crossref","first-page":"628","DOI":"10.3201\/eid2603.191417","article-title":"Chlamydia abortus in pregnant woman with acute respiratory distress syndrome","volume":"26","author":"Pichon","year":"2020","journal-title":"Emerging Infect. Dis."},{"key":"10.1016\/j.jbc.2025.110940_bib6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s13567-022-01052-x","article-title":"Recent advances and public health implications for environmental exposure to Chlamydia abortus: from enzootic to zoonotic disease","volume":"53","author":"Turin","year":"2022","journal-title":"Vet. Res."},{"key":"10.1016\/j.jbc.2025.110940_bib7","doi-asserted-by":"crossref","first-page":"3561","DOI":"10.2147\/IDR.S469837","article-title":"A case Study of severe pneumonia caused by mixed infection of Chlamydia abortus and influenza a in a female patient","volume":"17","author":"Ye","year":"2024","journal-title":"Infect Drug Resist"},{"key":"10.1016\/j.jbc.2025.110940_bib8","article-title":"Severe community-acquired pneumonia caused by Chlamydia abortus in China: a case report","volume":"11","author":"Yang","year":"2024","journal-title":"Front Med. (Lausanne)"},{"key":"10.1016\/j.jbc.2025.110940_bib9","doi-asserted-by":"crossref","first-page":"630","DOI":"10.3201\/eid3103.241406","article-title":"Community-acquired pneumonia caused by avain Chlamydia abortus","volume":"31","author":"Gooskens","year":"2025","journal-title":"Emerging Infect. Dis."},{"key":"10.1016\/j.jbc.2025.110940_bib10","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/S1473-3099(24)00529-2","article-title":"Circulation of avian Chlamydia abortus in the Netherlands and community-acquired pneumonia: an outbreak investigation and retrospective cohort study","volume":"25","author":"Raven","year":"2025","journal-title":"Lancet Infect Dis."},{"key":"10.1016\/j.jbc.2025.110940_bib11","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.femsre.2005.03.002","article-title":"The chlamydial developmental cycle","volume":"29","author":"Abdelrahman","year":"2005","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.jbc.2025.110940_bib12","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1177\/0300985813505114","article-title":"Techniques in electron microscopy of animal tissue","volume":"51","author":"Cheville","year":"2014","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.jbc.2025.110940_bib13","series-title":"Nanotechnology Characterization Tools for Biosensing and Medical Diagnosis","first-page":"277","article-title":"Label-Free raman imaging","author":"Hobro","year":"2018"},{"key":"10.1016\/j.jbc.2025.110940_bib14","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1038\/nprot.2016.036","article-title":"Using Raman spectroscopy to characterize biological materials","volume":"11","author":"Butler","year":"2016","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jbc.2025.110940_bib15","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1002\/jrs.4844","article-title":"The application of Raman spectroscopy for the detection and identification of microorganisms","volume":"47","author":"St\u00f6ckel","year":"2016","journal-title":"J. Raman Spectrosc."},{"key":"10.1016\/j.jbc.2025.110940_bib16","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1111\/j.1365-2958.2010.07241.x","article-title":"Raman microspectroscopy reveals long-term extracellular activity of chlamydiae","volume":"77","author":"Haider","year":"2010","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jbc.2025.110940_bib17","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1038\/s43586-021-00075-6","article-title":"Raman microspectroscopy for microbiology","volume":"1","author":"Lee","year":"2021","journal-title":"Nat. Rev. Methods Primers"},{"key":"10.1016\/j.jbc.2025.110940_bib18","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.micinf.2013.03.005","article-title":"Characterizing the intracellular distribution of metabolites in intact Chlamydia-infected cells by Raman and two-photon microscopy","volume":"15","author":"Szaszak","year":"2013","journal-title":"Microbes Infect"},{"key":"10.1016\/j.jbc.2025.110940_bib19","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1021\/ac503316s","article-title":"Label-free imaging and spectroscopic analysis of intracellular bacterial infections","volume":"87","author":"Gro\u00dfe","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jbc.2025.110940_bib20","doi-asserted-by":"crossref","first-page":"4988","DOI":"10.1021\/acs.analchem.1c04754","article-title":"Looking inside non-destructively: label-free, raman-based visualization of intracellular Coxiella burnetii","volume":"94","author":"Unger","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jbc.2025.110940_bib21","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1093\/jmicro\/dfae011","article-title":"Chlamydia psittaci infected cell studies by 4Pi Raman and atomic force microscopy","volume":"73","author":"Khalenkow","year":"2024","journal-title":"Microscopy"},{"key":"10.1016\/j.jbc.2025.110940_bib22","series-title":"Biology of Chlamydia","first-page":"35","article-title":"One face of Chlamydia trachomatis: the infectious elementary body","author":"Coss\u00e9","year":"2018"},{"key":"10.1016\/j.jbc.2025.110940_bib23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1008878","article-title":"The Chlamydia trachomatis secreted effector TmeA hijacks the N-WASP-ARP2\/3 actin remodeling axis to facilitate cellular invasion","volume":"16","author":"Faris","year":"2020","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.jbc.2025.110940_bib24","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1111\/1574-6976.12059","article-title":"Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities","volume":"38","author":"Omsland","year":"2014","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.jbc.2025.110940_bib25","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/s41579-023-00860-y","article-title":"Intracellular lifestyle of Chlamydia trachomatis and host\u2013pathogen interactions","volume":"21","author":"Stelzner","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jbc.2025.110940_bib26","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1002\/cphc.200600507","article-title":"Towards a detailed understanding of bacterial metabolism--spectroscopic characterization of Staphylococcus epidermidis","volume":"8","author":"Neugebauer","year":"2007","journal-title":"Chemphyschem"},{"key":"10.1016\/j.jbc.2025.110940_bib27","doi-asserted-by":"crossref","first-page":"3590","DOI":"10.1039\/C6AN00152A","article-title":"Raman spectroscopy: an evolving technique for live cell studies","volume":"141","author":"Smith","year":"2016","journal-title":"Analyst"},{"key":"10.1016\/j.jbc.2025.110940_bib28","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.3390\/genes13112127","article-title":"Recent advances in raman spectral imaging in cell diagnosis and gene expression prediction","volume":"13","author":"Watanabe","year":"2022","journal-title":"Genes"},{"key":"10.1016\/j.jbc.2025.110940_bib29","first-page":"895","article-title":"Elektronenmikroskopische Schnelldiagnostik an Gewebeproben bei Infektionskrankheiten","volume":"6","author":"Bergmann","year":"1991","journal-title":"Med. Techn Assist"},{"key":"10.1016\/j.jbc.2025.110940_bib30","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.trac.2015.12.001","article-title":"An overview of emerging hyphenated SEM-EDX and Raman spectroscopy systems: applications in life, environmental and materials sciences","volume":"77","author":"Cardell","year":"2016","journal-title":"Trac Trends Anal. Chem."},{"key":"10.1016\/j.jbc.2025.110940_bib31","series-title":"Confocal Raman Microscopy","year":"2018"},{"key":"10.1016\/j.jbc.2025.110940_bib32","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.morpho.2018.12.003","article-title":"Confocal Raman microscopy in life sciences","volume":"103","author":"Gomes da Costa","year":"2019","journal-title":"Morphologie"},{"key":"10.1016\/j.jbc.2025.110940_bib33","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.virol.2008.05.001","article-title":"Behind the chlamydial cloak: the replication cycle of chlamydiaphage Chp2 revealed","volume":"377","author":"Salim","year":"2008","journal-title":"Virology"},{"key":"10.1016\/j.jbc.2025.110940_bib34","series-title":"R: A Language and Environment for Statistical Computing","author":"Team","year":"2025"},{"key":"10.1016\/j.jbc.2025.110940_bib35","series-title":"hyperSpec: a package to handle hyperspectral data sets in R","author":"Beleites","year":"2018"},{"key":"10.1016\/j.jbc.2025.110940_bib36","series-title":"Modern Applied Statistics with S","year":"2002"},{"key":"10.1016\/j.jbc.2025.110940_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chemolab.2016.03.024","article-title":"Automatization of spike correction in Raman spectra of biological samples","volume":"155","author":"Ryabchykov","year":"2016","journal-title":"Chemometrics Intell. Lab. Syst."},{"key":"10.1016\/j.jbc.2025.110940_bib39","series-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2016"},{"key":"10.1016\/j.jbc.2025.110940_bib40","series-title":"Cowplot: streamlined plot theme and plot annotations for \u2018ggplot2\u2019 (Wilkelab\/cowplot: 1.2.0)","author":"Wilke","year":"2024"},{"key":"10.1016\/j.jbc.2025.110940_bib41","series-title":"ggh4x: Hacks for \u201cggplot2\u201d. R package version 0.3.1.9000","author":"van den Brand","year":"2025"},{"key":"10.1016\/j.jbc.2025.110940_bib42","series-title":"N-FINDR: an algorithm for fast autonomous spectral end-member determination in hyperspectral data","author":"Winter","year":"1999"},{"key":"10.1016\/j.jbc.2025.110940_bib43","doi-asserted-by":"crossref","first-page":"824","DOI":"10.3390\/ijms24010824","article-title":"Label-free characterization of macrophage polarization using raman spectroscopy","volume":"24","author":"Naumann","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbc.2025.110940_bib44","series-title":"Statistical Models","article-title":"Local regression models (chapter 8)","author":"Cleveland","year":"1992"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925825027929?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925825027929?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T08:37:15Z","timestamp":1766133435000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925825027929"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["S0021925825027929"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2025.110940","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Insights into chlamydial infection at the sub-cellular level using label-free Raman spectroscopy in comparison to electron microscopy","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2025.110940","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology\u00e9","name":"copyright","label":"Copyright"}],"article-number":"110940"}}