{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T00:06:43Z","timestamp":1793923603683,"version":"4.8.0"},"reference-count":34,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007707","name":"University of California, Davis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007707","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012870","name":"Ovarian Cancer Alliance of Greater Cincinnati","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012870","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Institutes of Health National Cancer Institute","doi-asserted-by":"publisher","award":["R01CA233752"],"award-info":[{"award-number":["R01CA233752"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007862","name":"California National Primate Research Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007862","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"US Department of Defense","doi-asserted-by":"publisher","award":["OC180412"],"award-info":[{"award-number":["OC180412"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.jbc.2025.110962","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T17:28:59Z","timestamp":1763659739000},"page":"110962","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["An evolutionary T119R substitution in the macaque T-cell immunoreceptor TIGIT enables proteolytic shedding and reveals clinical antibody vulnerabilities"],"prefix":"10.1016","volume":"302","author":[{"given":"Eric","family":"Pirillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Freenor V","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brice E.N.","family":"Wamba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanchita","family":"Bhatnagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanmoy","family":"Mondal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7898-7218","authenticated-orcid":false,"given":"Jogender","family":"Tushir-Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2025.110962_bib1","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1016\/j.ccell.2021.07.007","article-title":"The CD155\/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer","volume":"39","author":"Freed-Pastor","year":"2021","journal-title":"Cancer Cell"},{"key":"10.1016\/j.jbc.2025.110962_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21030922","article-title":"CD155: a multi-functional molecule in tumor progression","volume":"21","author":"Molfetta","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbc.2025.110962_bib3","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1056\/NEJMoa063842","article-title":"Cytokine storm in a phase 1 trial of the anti-CD28 monoclonal antibody TGN1412","volume":"355","author":"Suntharalingam","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jbc.2025.110962_bib4","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1200\/JCO.23.01363","article-title":"SKYSCRAPER-02: tiragolumab in combination with atezolizumab plus chemotherapy in untreated extensive-stage small-cell lung cancer","volume":"42","author":"Rudin","year":"2024","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.jbc.2025.110962_bib5","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.annonc.2021.11.002","article-title":"First-in-human phase 1 study of the anti-TIGIT antibody vibostolimab as monotherapy or with pembrolizumab for advanced solid tumors, including non-small-cell lung cancer","volume":"33","author":"Niu","year":"2022","journal-title":"Ann. Oncol."},{"key":"10.1016\/j.jbc.2025.110962_bib6","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/S1470-2045(24)00679-X","article-title":"Tiragolumab in combination with atezolizumab and bevacizumab in patients with unresectable, locally advanced or metastatic hepatocellular carcinoma (MORPHEUS-Liver): a randomised, open-label, phase 1b-2, study","volume":"26","author":"Finn","year":"2025","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.jbc.2025.110962_bib7","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1007\/s00262-025-04128-7","article-title":"Anti-TIGIT therapies: a review of preclinical and clinical efficacy and mechanisms","volume":"74","author":"Sundstrom","year":"2025","journal-title":"Cancer Immunol. Immunother."},{"key":"10.1016\/j.jbc.2025.110962_bib8","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1471-2164-7-15","article-title":"Comparative analysis of cancer genes in the human and chimpanzee genomes","volume":"7","author":"Puente","year":"2006","journal-title":"BMC Genomics"},{"key":"10.1016\/j.jbc.2025.110962_bib9","doi-asserted-by":"crossref","first-page":"5748","DOI":"10.1038\/s41467-025-60990-0","article-title":"Evolutionary regulation of human Fas ligand (CD95L) by plasmin in solid cancer immunotherapy","volume":"16","author":"Wamba","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2025.110962_bib10","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1172\/JCI35382","article-title":"The calm after the cytokine storm: lessons from the TGN1412 trial","volume":"118","author":"St Clair","year":"2008","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jbc.2025.110962_bib11","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1160\/TH04-12-0774","article-title":"The role of CD40 in CD40L- and antibody-mediated platelet activation","volume":"93","author":"Langer","year":"2005","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.jbc.2025.110962_bib12","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1111\/ajt.14197","article-title":"Fc-Silent Anti-CD154 domain antibody effectively prevents Nonhuman primate renal allograft rejection","volume":"17","author":"Kim","year":"2017","journal-title":"Am. J. Transpl."},{"key":"10.1016\/j.jbc.2025.110962_bib13","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1182\/blood-2015-09-670117","article-title":"Natural heterogeneity of alpha2-antiplasmin: functional and clinical consequences","volume":"127","author":"Abdul","year":"2016","journal-title":"Blood"},{"key":"10.1016\/j.jbc.2025.110962_bib14","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/j.1365-2141.2005.05444.x","article-title":"Molecular mechanisms of fibrinolysis","volume":"129","author":"Cesarman-Maus","year":"2005","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.jbc.2025.110962_bib15","doi-asserted-by":"crossref","first-page":"5374","DOI":"10.1073\/pnas.90.11.5374","article-title":"Breaching the conformational integrity of the catalytic triad of the serine protease plasmin: localized disruption of a side chain of His-603 strongly inhibits the amidolytic activity of human plasmin","volume":"90","author":"Mhashilkar","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2025.110962_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0054104","article-title":"The structure of human microplasmin in complex with textilinin-1, an aprotinin-like inhibitor from the Australian brown snake","volume":"8","author":"Millers","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jbc.2025.110962_bib17","doi-asserted-by":"crossref","first-page":"5399","DOI":"10.1073\/pnas.1120606109","article-title":"Structure of TIGIT immunoreceptor bound to poliovirus receptor reveals a cell-cell adhesion and signaling mechanism that requires cis-trans receptor clustering","volume":"109","author":"Stengel","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2025.110962_bib18","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1161\/01.CIR.96.2.569","article-title":"Aprotinin inhibits plasmin-induced platelet activation during cardiopulmonary bypass","volume":"96","author":"Shigeta","year":"1997","journal-title":"Circulation"},{"key":"10.1016\/j.jbc.2025.110962_bib19","doi-asserted-by":"crossref","first-page":"5016","DOI":"10.1038\/s41467-023-40755-3","article-title":"TIGIT can inhibit T cell activation via ligation-induced nanoclusters, independent Cd226 Co-stimulation","volume":"14","author":"Worboys","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2025.110962_bib20","doi-asserted-by":"crossref","DOI":"10.1080\/19420862.2023.2253788","article-title":"Biophysical characterization of PVR family interactions and therapeutic antibody recognition to TIGIT","volume":"15","author":"Diong","year":"2023","journal-title":"MAbs"},{"key":"10.1016\/j.jbc.2025.110962_bib21","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1038\/s41419-025-07984-4","article-title":"TIGIT in cancer: from mechanism of action to promising immunotherapeutic strategies","volume":"16","author":"Cui","year":"2025","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.jbc.2025.110962_bib22","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.cell.2019.02.016","article-title":"Suppression of exosomal PD-L1 induces systemic anti-tumor immunity and memory","volume":"177","author":"Poggio","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2025.110962_bib23","article-title":"An Fc-Competent anti-human TIGIT blocking antibody ociperlimab (BGB-A1217) elicits strong immune responses and potent anti-tumor efficacy in pre-clinical models","volume":"13","author":"Chen","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jbc.2025.110962_bib24","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1158\/0008-5472.CAN-23-2455","article-title":"Fc-Silent Anti-TIGIT antibodies potentiate antitumor immunity without depleting regulatory T cells","volume":"84","author":"Piovesan","year":"2024","journal-title":"Cancer Res."},{"key":"10.1016\/j.jbc.2025.110962_bib25","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/s41586-024-07121-9","article-title":"Anti-TIGIT antibody improves PD-L1 blockade through myeloid and T(reg)","volume":"627","author":"Guan","year":"2024","journal-title":"Cell Nat."},{"key":"10.1016\/j.jbc.2025.110962_bib26","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1111\/j.1600-0684.1997.tb00042.x","article-title":"Advantages and limitations of nonhuman primates as animal models in genetic research on complex diseases","volume":"26","author":"VandeBerg","year":"1997","journal-title":"J. Med. Primatol."},{"key":"10.1016\/j.jbc.2025.110962_bib27","doi-asserted-by":"crossref","first-page":"3885","DOI":"10.1038\/s41598-020-60436-1","article-title":"Interspecies comparison of simultaneous thrombin and plasmin generation","volume":"10","author":"Tarandovskiy","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbc.2025.110962_bib28","first-page":"269","article-title":"Effects of hepatic ischemia on coagulation in primates. Application to liver transplantation","volume":"68","author":"Dinbar","year":"1970","journal-title":"Surgery"},{"key":"10.1016\/j.jbc.2025.110962_bib29","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1001\/jamaoncol.2023.3867","article-title":"Anti-TIGIT antibody tiragolumab alone or with atezolizumab in patients with advanced solid tumors: a phase 1a\/1b nonrandomized controlled trial","volume":"9","author":"Kim","year":"2023","journal-title":"JAMA Oncol."},{"key":"10.1016\/j.jbc.2025.110962_bib30","doi-asserted-by":"crossref","DOI":"10.3390\/antib13030056","article-title":"Immune-related adverse events associated with atezolizumab: insights from real-world pharmacovigilance data","author":"Frey","year":"2024","journal-title":"Antibodies (Basel)"},{"key":"10.1016\/j.jbc.2025.110962_bib31","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms12997","article-title":"Genome sequence of the basal haplorrhine primate Tarsius syrichta reveals unusual insertions","volume":"7","author":"Schmitz","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2025.110962_bib32","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1101\/gr.255174.119","article-title":"Paternal age in rhesus macaques is positively associated with germline mutation accumulation but not with measures of offspring sociability","volume":"30","author":"Wang","year":"2020","journal-title":"Genome Res."},{"key":"10.1016\/j.jbc.2025.110962_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000838","article-title":"A comparison of humans and baboons suggests germline mutation rates do not track cell divisions","volume":"18","author":"Wu","year":"2020","journal-title":"Plos Biol."},{"key":"10.1016\/j.jbc.2025.110962_bib34","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.ccell.2018.07.005","article-title":"A single-agent dual-specificity targeting of FOLR1 and DR5 as an effective strategy for ovarian cancer","volume":"34","author":"Shivange","year":"2018","journal-title":"Cancer Cell"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925825028145?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925825028145?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T09:00:20Z","timestamp":1773910820000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925825028145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["S0021925825028145"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2025.110962","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An evolutionary T119R substitution in the macaque T-cell immunoreceptor TIGIT enables proteolytic shedding and reveals clinical antibody vulnerabilities","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2025.110962","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology\u00e9","name":"copyright","label":"Copyright"}],"article-number":"110962"}}