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Med."},{"key":"10.1016\/j.jbc.2026.111353_bib84","doi-asserted-by":"crossref","DOI":"10.1186\/s12974-020-01826-0","article-title":"IC100: a novel anti-ASC monoclonal antibody improves functional outcomes in an animal model of multiple sclerosis","author":"Desu","year":"2020","journal-title":"J Neuroinflammation"},{"key":"10.1016\/j.jbc.2026.111353_bib85","doi-asserted-by":"crossref","DOI":"10.1038\/s41419-021-04420-1","article-title":"Identification of an ASC oligomerization inhibitor for the treatment of inflammatory diseases","author":"Soriano-Teruel","year":"2021","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.jbc.2026.111353_bib86","doi-asserted-by":"crossref","DOI":"10.1186\/s12974-022-02682-w","article-title":"Directly targeting ASC by lonidamine alleviates inflammasome-driven diseases","author":"Chen","year":"2022","journal-title":"J Neuroinflammation"},{"key":"10.1016\/j.jbc.2026.111353_bib87","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2022.102696","article-title":"Spirodalesol analog 8A inhibits NLRP3 inflammasome activation and attenuates inflammatory disease by directly targeting adaptor protein ASC","author":"Liu","year":"2022","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbc.2026.111353_bib88","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/gene.2014.30","article-title":"The CLRX.1\/NOD24 (NLRP2P) pseudogene codes a functional negative regulator of NF-\u03baB, pyrin-only protein 4","volume":"15","author":"Porter","year":"2014","journal-title":"Genes Immun."},{"key":"10.1016\/j.jbc.2026.111353_bib89","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/imr.12290","article-title":"Inhibiting the inflammasome: one domain at a time","volume":"265","author":"Dorfleutner","year":"2015","journal-title":"Immunol. 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Chem."},{"key":"10.1016\/j.jbc.2026.111353_bib102","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1-2","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"key":"10.1016\/j.jbc.2026.111353_bib103","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.1002\/prot.22711","article-title":"Improved side-chain torsion potentials for the amber ff99SB protein force field","volume":"8","author":"Lindorff-Larsen","year":"2010","journal-title":"Proteins"},{"key":"10.1016\/j.jbc.2026.111353_bib104","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1063\/1.445869","article-title":"Comparison of simple potential functions for simulating liquid water","volume":"79","author":"Jorgensen","year":"1983","journal-title":"J. Chem. 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