{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:12:08Z","timestamp":1790107928772,"version":"4.0.1"},"reference-count":49,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Hunan Provincial Natural Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014472","name":"Scientific Research Foundation of Hunan Provincial Education Department","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014472","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.jbc.2026.113347","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:53:01Z","timestamp":1784677981000},"page":"113347","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular and structural dissection of Psalmopoeus pulcher venom reveals cysteine-rich peptides that target acid-sensing ion channel 1a"],"prefix":"10.1016","volume":"302","author":[{"given":"Zhaotun","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiatian","family":"Quan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongfang","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingxin","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichong","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7121-436X","authenticated-orcid":false,"given":"Zhen","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2026.113347_bib1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/brv.12793","article-title":"The biology and evolution of spider venoms","volume":"97","author":"Luddecke","year":"2022","journal-title":"Biol. Rev. Camb. Philos. Soc."},{"key":"10.1016\/j.jbc.2026.113347_bib2","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1111\/brv.13120","article-title":"Prey specificity of predatory venoms","volume":"99","author":"Michalek","year":"2024","journal-title":"Biol. Rev. Camb. Philos. Soc."},{"key":"10.1016\/j.jbc.2026.113347_bib3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/s41573-019-0013-8","article-title":"Antibodies and venom peptides: new modalities for ion channels","volume":"18","author":"Wulff","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jbc.2026.113347_bib4","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3390\/molecules29010035","article-title":"Spider-Venom peptides: structure, bioactivity, strategy, and research applications","volume":"29","author":"Guo","year":"2023","journal-title":"Molecules"},{"key":"10.1016\/j.jbc.2026.113347_bib5","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1586\/epr.09.45","article-title":"Venomics as a drug discovery platform","volume":"6","author":"Escoubas","year":"2009","journal-title":"Expert Rev. Proteomic."},{"key":"10.1016\/j.jbc.2026.113347_bib6","doi-asserted-by":"crossref","first-page":"6061","DOI":"10.3390\/molecules29246061","article-title":"In Silico Conotoxin studies: progress and prospects","volume":"29","author":"Li","year":"2024","journal-title":"Molecules"},{"key":"10.1016\/j.jbc.2026.113347_bib7","doi-asserted-by":"crossref","DOI":"10.1093\/femsre\/fuab052","article-title":"Phage display and other peptide display technologies","volume":"46","author":"Jaroszewicz","year":"2022","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.jbc.2026.113347_bib8","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.ejcb.2017.06.003","article-title":"Yeast-based assays for detecting protein-protein\/drug interactions and their inhibitors","volume":"96","author":"Moosavi","year":"2017","journal-title":"Eur. J. Cell Biol."},{"key":"10.1016\/j.jbc.2026.113347_bib9","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/nature05285","article-title":"Spider toxins activate the capsaicin receptor to produce inflammatory pain","volume":"444","author":"Siemens","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113347_bib10","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.cell.2004.08.026","article-title":"Neuroprotection in ischemia: blocking calcium-permeable acid-sensing ion channels","volume":"118","author":"Xiong","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2026.113347_bib11","doi-asserted-by":"crossref","first-page":"25116","DOI":"10.1074\/jbc.M003643200","article-title":"Isolation of a tarantula toxin specific for a class of proton-gated Na+ channels","volume":"275","author":"Escoubas","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbc.2026.113347_bib12","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1038\/ncomms1917","article-title":"Structure of the acid-sensing ion channel 1 in complex with the gating modifier Psalmotoxin 1","volume":"3","author":"Dawson","year":"2012","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbc.2026.113347_bib13","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1038\/nn1940","article-title":"A tarantula peptide against pain via ASIC1a channels and opioid mechanisms","volume":"10","author":"Mazzuca","year":"2007","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.jbc.2026.113347_bib14","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.ejpain.2010.08.005","article-title":"Spinal cord plasticity and acid-sensing ion channels involvement in a rodent model of irritable bowel syndrome","volume":"15","author":"Matricon","year":"2011","journal-title":"Eur. J. Pain."},{"key":"10.1016\/j.jbc.2026.113347_bib15","doi-asserted-by":"crossref","DOI":"10.1590\/1678-9199-jvatitd-2021-0026","article-title":"Pain-related toxins in scorpion and spider venoms: a face to face with ion channels","volume":"27","author":"Diochot","year":"2021","journal-title":"J. Venom Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.jbc.2026.113347_bib16","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1038\/s42003-025-09015-6","article-title":"Comparative venomics suggests an evolutionary adaption of spider venom from predation to defense","volume":"8","author":"L\u00fcddecke","year":"2025","journal-title":"Commun. Biol."},{"key":"10.1016\/j.jbc.2026.113347_bib17","article-title":"W\u00f6rterbuch der Wirbellosen\/Dictionary of Invertebrates: latein-deutsch-englisch","author":"Cole","year":"2016","journal-title":"Springer-Verlag"},{"key":"10.1016\/j.jbc.2026.113347_bib18","doi-asserted-by":"crossref","first-page":"611","DOI":"10.3390\/toxins11100611","article-title":"Spider venom: components, modes of action, and novel strategies in transcriptomic and proteomic analyses","volume":"11","author":"Langenegger","year":"2019","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.jbc.2026.113347_bib19","doi-asserted-by":"crossref","first-page":"140","DOI":"10.3390\/toxins14020140","article-title":"Molecular diversity of peptide toxins in the venom of spider Heteropoda pingtungensis as revealed by cDNA Library and transcriptome sequencing analysis","volume":"14","author":"Liao","year":"2022","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.jbc.2026.113347_bib20","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/0896-6273(95)90184-1","article-title":"An inhibitor of the Kv2. 1 potassium channel isolated from the venom of a Chilean tarantula","volume":"15","author":"Swartz","year":"1995","journal-title":"Neuron"},{"key":"10.1016\/j.jbc.2026.113347_bib21","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/nature03873","article-title":"Voltage-sensor activation with a tarantula toxin as cargo","volume":"436","author":"Revell Phillips","year":"2005","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113347_bib22","doi-asserted-by":"crossref","first-page":"15591","DOI":"10.1021\/bi061457+","article-title":"Solution structure and functional characterization of jingzhaotoxin-XI: a novel gating modifier of both potassium and sodium channels","volume":"45","author":"Liao","year":"2006","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbc.2026.113347_bib23","doi-asserted-by":"crossref","first-page":"2550","DOI":"10.1021\/pr1000016","article-title":"Molecular diversification of peptide toxins from the tarantula Haplopelma hainanum (Ornithoctonus hainana) venom based on transcriptomic, peptidomic, and genomic analyses","volume":"9","author":"Tang","year":"2010","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.jbc.2026.113347_bib24","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.toxicon.2011.06.006","article-title":"Characterization of voltage-dependent calcium channel blocking peptides from the venom of the tarantula Grammostola rosea","volume":"58","author":"Ono","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.jbc.2026.113347_bib25","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1016\/j.toxicon.2008.08.018","article-title":"JZTX-IV, a unique acidic sodium channel toxin isolated from the spider Chilobrachys jingzhao","volume":"52","author":"Wang","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.jbc.2026.113347_bib26","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1007\/s00018-008-8135-x","article-title":"Molecular diversity and evolution of cystine knot toxins of the tarantula Chilobrachys jingzhao","volume":"65","author":"Chen","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.jbc.2026.113347_bib27","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.toxicon.2008.03.024","article-title":"Molecular diversification based on analysis of expressed sequence tags from the venom glands of the Chinese bird spider Ornithoctonus huwena","volume":"51","author":"Jiang","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.jbc.2026.113347_bib28","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1110\/ps.30502","article-title":"The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution","volume":"11","author":"Shu","year":"2002","journal-title":"Protein Sci."},{"key":"10.1016\/j.jbc.2026.113347_bib29","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.toxicon.2016.10.013","article-title":"Isolation of two insecticidal toxins from venom of the Australian theraphosid spider Coremiocnemis tropix","volume":"123","author":"Ikonomopoulou","year":"2016","journal-title":"Toxicon"},{"key":"10.1016\/j.jbc.2026.113347_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0084404","article-title":"A new theraphosid spider toxin causes early insect cell death by necrosis when expressed in vitro during recombinant baculovirus infection","volume":"8","author":"Ardisson-Ara\u00fajo","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jbc.2026.113347_bib31","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1124\/mol.111.072207","article-title":"A dynamic pharmacophore drives the interaction between Psalmotoxin-1 and the putative drug target acid-sensing ion channel 1a","volume":"80","author":"Saez","year":"2011","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.jbc.2026.113347_bib32","doi-asserted-by":"crossref","first-page":"4985","DOI":"10.1111\/bph.13267","article-title":"Molecular dynamics and functional studies define a hot spot of crystal contacts essential for PcTx1 inhibition of acid-sensing ion channel 1a","volume":"172","author":"Saez","year":"2015","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.jbc.2026.113347_bib33","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.bcp.2019.03.004","article-title":"The modulation of acid-sensing ion channel 1 by PcTx1 is pH-, subtype- and species-dependent: importance of interactions at the channel subunit interface and potential for engineering selective analogues","volume":"163","author":"Cristofori-Armstrong","year":"2019","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.jbc.2026.113347_bib34","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.210242","article-title":"Darwin's bark spider shares a spidroin repertoire with Caerostris extrusa but achieves extraordinary silk toughness through gene expression","volume":"11","author":"Kono","year":"2021","journal-title":"Open Biol."},{"key":"10.1016\/j.jbc.2026.113347_bib35","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.toxicon.2008.05.020","article-title":"A rational nomenclature for naming peptide toxins from spiders and other venomous animals","volume":"52","author":"King","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.jbc.2026.113347_bib36","doi-asserted-by":"crossref","first-page":"27899","DOI":"10.1074\/jbc.M109.029009","article-title":"Identification of protein domains that control proton and calcium sensitivity of ASIC1a","volume":"284","author":"Sherwood","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbc.2026.113347_bib37","doi-asserted-by":"crossref","first-page":"3180","DOI":"10.1038\/s44318-025-00438-9","article-title":"Inherent fast inactivation particle of Nav channels as a new binding site for a neurotoxin","volume":"44","author":"Zhou","year":"2025","journal-title":"EMBO J."},{"key":"10.1016\/j.jbc.2026.113347_bib38","doi-asserted-by":"crossref","first-page":"3750","DOI":"10.1073\/pnas.1614728114","article-title":"Potent neuroprotection after stroke afforded by a double-knot spider-venom peptide that inhibits acid-sensing ion channel 1a","volume":"114","author":"Chassagnon","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbc.2026.113347_bib39","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/731293","article-title":"Molecular cloning and sequence analysis of the cDNAs encoding toxin-like peptides from the venom glands of tarantula Grammostola rosea","volume":"2012","author":"Kimura","year":"2012","journal-title":"Int. J. Pept."},{"key":"10.1016\/j.jbc.2026.113347_bib40","doi-asserted-by":"crossref","first-page":"evab185","DOI":"10.1093\/gbe\/evab185","article-title":"Evolution of the insect PPK gene family","volume":"13","author":"Latorre-Estivalis","year":"2021","journal-title":"Genome Biol. Evol."},{"key":"10.1016\/j.jbc.2026.113347_bib41","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.cub.2009.12.057","article-title":"Pickpocket is a DEG\/ENaC protein required for mechanical nociception in Drosophila larvae","volume":"20","author":"Zhong","year":"2010","journal-title":"Curr. Biol."},{"key":"10.1016\/j.jbc.2026.113347_bib42","doi-asserted-by":"crossref","first-page":"39878","DOI":"10.1074\/jbc.M112.411736","article-title":"Pickpocket1 is an ionotropic molecular sensory transducer","volume":"287","author":"Boiko","year":"2012","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbc.2026.113347_bib43","article-title":"Molecular diversity of peptides from Pandercetes sp. spider venom and antagonistic activity on voltage-gated calcium channels of the kappa-LhTx-1 toxin","volume":"337","author":"Tang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.jbc.2026.113347_bib44","first-page":"71","article-title":"DNASTAR\u2019s Lasergene sequence analysis software","volume":"132","author":"Burland","year":"2000","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jbc.2026.113347_bib45","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1002\/prot.20390","article-title":"A novel strategy for the identification of toxinlike structures in spider venom","volume":"59","author":"Kozlov","year":"2005","journal-title":"Proteins"},{"key":"10.1016\/j.jbc.2026.113347_bib46","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.toxicon.2013.08.054","article-title":"Characterization of the peptidylglycine alpha-amidating monooxygenase (PAM) from the venom ducts of neogastropods, Conus bullatus and Conus geographus","volume":"74","author":"Ul-Hasan","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.jbc.2026.113347_bib47","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113347_bib48","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejphar.2024.177213","article-title":"Pore blocking mechanisms of centipede toxin SsTx-4 on the inwardly rectifying potassium channels","volume":"988","author":"Tang","year":"2025","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.jbc.2026.113347_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2021.100326","article-title":"Engineering of highly potent and selective HNTX-III mutant against hNa(v)1.7 sodium channel for treatment of pain","volume":"296","author":"Zhang","year":"2021","journal-title":"J. Biol. Chem."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925826022192?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925826022192?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:53:08Z","timestamp":1790106788000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925826022192"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":49,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S0021925826022192"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2026.113347","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular and structural dissection of Psalmopoeus pulcher venom reveals cysteine-rich peptides that target acid-sensing ion channel 1a","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2026.113347","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"113347"}}