{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:49:01Z","timestamp":1792093741207,"version":"4.4.0"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:00:00Z","timestamp":1791590400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.jbc.2026.113649","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T15:09:35Z","timestamp":1791990575000},"page":"113649","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural and Kinetic Basis for Selective Inhibition of Canine Myeloperoxidase by Staphylococcal Peroxidase Inhibitor (SPIN) Orthologs"],"prefix":"10.1016","author":[{"given":"Soheila","family":"Fatehi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Herdendorf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Burklund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trisha","family":"Kvasnica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Lindquist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatem","family":"Kittana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1775-0727","authenticated-orcid":false,"given":"Brian V.","family":"Geisbrecht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2026.113649_bib1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/j.1600-065X.2007.00550.x","article-title":"How Human Neutrophils Kill and Degrade Microbes: an Integrated View","volume":"219","author":"Nauseef","year":"2007","journal-title":"Immunol Rev"},{"key":"10.1016\/j.jbc.2026.113649_bib2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1146\/annurev-immunol-020711-074942","article-title":"Neutrophil Function: From Mechanisms to Disease","volume":"30","author":"Amulic","year":"2012","journal-title":"Annu Rev Immunol"},{"key":"10.1016\/j.jbc.2026.113649_bib3","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/j.micinf.2003.09.008","article-title":"Neutrophil Granules and Secretory Vesicles","volume":"5","author":"Faurschou","year":"2003","journal-title":"Inflammation Microbes Infect"},{"key":"10.1016\/j.jbc.2026.113649_bib4","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1189\/jlb.0712349","article-title":"Myeloperoxidase: a Front-Line Defender Against Phagocytosed","volume":"93","author":"Klebanoff","year":"2013","journal-title":"Microorganisms J Leukocyte Biol"},{"key":"10.1016\/j.jbc.2026.113649_bib5","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1111\/cmi.12312","article-title":"Myeloperoxidase in Human Neutrophil Host Defense","volume":"16","author":"Nauseef","year":"2014","journal-title":"Cell Microbiol"},{"key":"10.1016\/j.jbc.2026.113649_bib6","doi-asserted-by":"crossref","first-page":"39860","DOI":"10.1074\/jbc.M605898200","article-title":"Modeling the Reactions of Superoxide and Myeloperoxidase in the Neutrophil Phagosome","volume":"281","author":"Winterbourn","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jbc.2026.113649_bib7","doi-asserted-by":"crossref","first-page":"9439","DOI":"10.1073\/pnas.1707032114","article-title":"Immune Evasion by a Staphylococal Inhibitor of Myeloperoxidase","volume":"114","author":"de Jong","year":"2017","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.jbc.2026.113649_bib8","doi-asserted-by":"crossref","unstructured":"Allison, M., Mishra, N., and Geisbrecht, B. V. (2022) Structure, Function, and Mechanistic Insights into a Novel Family of Myeloperoxidase Inhibitory Proteins Expressed by Staphylococci In Mammalian Heme Peroxidases: Diverse Roles in Health and Disease, Hawkins CL, Nauseef WM, eds. CRC Press, Boca Raton, FL Chapter 18, 307-319","DOI":"10.1201\/9781003212287-23"},{"key":"10.1016\/j.jbc.2026.113649_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.abb.2024.110177","article-title":"New Advances in Understanding Inhibition of Myeloperoxidase and Neutorphil Serine Proteases by Two Families of Staphylococcal Innate Immune Evasion Proteins","volume":"761","author":"Herdendorf","year":"2024","journal-title":"Arch Biochem Biophys"},{"key":"10.1016\/j.jbc.2026.113649_bib10","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.1074\/jbc.RA117.000134","article-title":"A Structurally Dynamic N-terminal Region Drives Function of the Staphylococcal Peroxidase Inhibitor (SPIN)","volume":"293","author":"de Jong","year":"2018","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jbc.2026.113649_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2022.102514","article-title":"The Staphylococcal Inhibitory Protein SPIN Binds to Human Myeloperoxidase with Picomolar Affinity but Only Dampens Halide Oxidation","volume":"298","author":"Leitgeb","year":"2022","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jbc.2026.113649_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.abb.2018.03.007","article-title":"Identification and Structural Characterization of a Novel Myeloperoxidase Inhibitor from Staphylococcus delphini","volume":"645","author":"Ploscariu","year":"2018","journal-title":"Arch Biochem Biophys"},{"key":"10.1016\/j.jbc.2026.113649_bib13","doi-asserted-by":"crossref","first-page":"2770","DOI":"10.1128\/JCM.00360-07","article-title":"Reclassification of phenotypically identified staphylococcus intermedius strains","volume":"45","author":"Sasaki","year":"2007","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jbc.2026.113649_bib14","article-title":"Are Staphylococcus intermedius Infections in Humans Cases of Mistaken Identity?","volume":"2","author":"Viau","year":"2015","journal-title":"A Case Series and Literature Review Open Forum Infectious Diseases"},{"key":"10.1016\/j.jbc.2026.113649_bib15","doi-asserted-by":"crossref","first-page":"4609","DOI":"10.1128\/JCM.01308-06","article-title":"First case of Staphylococcus pseudintermedius infection in a human","volume":"44","author":"Van Hoovels","year":"2006","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jbc.2026.113649_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.abb.2024.110023","article-title":"Staphylococcal Peroxidase Inhibitor (SPIN): Residue-Level Investigation of the Helical Bundle Domain","volume":"756","author":"Fatehi","year":"2024","journal-title":"Arch Biochem Biophys"},{"key":"10.1016\/j.jbc.2026.113649_bib17","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1146\/annurev-micro-092412-155746","article-title":"Neutrophils versus Staphylococcus aureus: a Biological Tug of War","volume":"67","author":"Spaan","year":"2013","journal-title":"Annu Rev Microbiol"},{"key":"10.1016\/j.jbc.2026.113649_bib18","doi-asserted-by":"crossref","unstructured":"de Jong, N. W. M., van Kessel, K. P. M., and van Strijp, J. A. G. (2019) Immune Evasion by Staphylococcus aureus Microbiol Spectr 7, GPP3-0061-2019,","DOI":"10.1128\/microbiolspec.GPP3-0061-2019"},{"key":"10.1016\/j.jbc.2026.113649_bib19","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/0022-2836(89)90143-5","article-title":"Tetragonal crystals of canine myeloperoxidase suitable for X-ray structural analysis","volume":"210","author":"Zeng","year":"1989","journal-title":"J Mol Biol"},{"key":"10.1016\/j.jbc.2026.113649_bib20","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0005-2795(77)90181-7","article-title":"The Subunit Structure of Crystalline Canine Myeloperoxidase","volume":"493","author":"Harrison","year":"1977","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.jbc.2026.113649_bib21","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0005-2744(78)90101-8","article-title":"Isolation procedure and some properties of myeloperoxidase from human leucocytes","volume":"524","author":"Bakkenist","year":"1978","journal-title":"Biochimica et Biophysica Acta (BBA) - Enzymology"},{"key":"10.1016\/j.jbc.2026.113649_bib22","doi-asserted-by":"crossref","first-page":"4211","DOI":"10.1016\/S0021-9258(19)69420-2","article-title":"The reductive cleavage of myeloperoxidase in half, producing enzymically active hemi-myeloperoxidase","volume":"256","author":"Andrews","year":"1981","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.jbc.2026.113649_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.abb.2024.110060","article-title":"Staphylococcal Peroxidase Inhibitor (SPIN): Investigation of the Inhibitory N-terminal Domain via a Stabilizing Disulfide Insertion","volume":"758","author":"Fatehi","year":"2024","journal-title":"Arch Biochem Biophys"},{"key":"10.1016\/j.jbc.2026.113649_bib24","article-title":"Exploring the Interaction of Biotinylated FcGamma RI and IgG1 Monoclonal Antibodies on Streptavidin-Coated Plasmonic Sensor Chips for Label-Free VEGF","volume":"14","author":"Khaligh","year":"2024","journal-title":"Detection Biosensors (Basel)"},{"key":"10.1016\/j.jbc.2026.113649_bib25","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1039\/D4CB00130C","article-title":"Bispecific FpFs: a versatile tool for preclinical antibody development","volume":"5","author":"Collins","year":"2024","journal-title":"RSC Chem Biol"},{"key":"10.1016\/j.jbc.2026.113649_bib26","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.abb.2005.09.017","article-title":"Active site structure and catalytic mechanisms of human peroxidases","volume":"445","author":"Furtm\u00fcller","year":"2006","journal-title":"Arch Biochem Biophys"},{"key":"10.1016\/j.jbc.2026.113649_bib27","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.jmb.2007.05.022","article-title":"Inference of Macromolecular Assemblies from Crystalline State","volume":"372","author":"Krissinel","year":"2007","journal-title":"J Mol Biol"},{"key":"10.1016\/j.jbc.2026.113649_bib28","doi-asserted-by":"crossref","unstructured":"Geisbrecht, B. V., Bouyain, S., and Pop, M. (2006) An Optimized System for the Expression and Purification of Secreted Bacterial Proteins Prot Expr Purif 46, 23-32,","DOI":"10.1016\/j.pep.2005.09.003"},{"key":"10.1016\/j.jbc.2026.113649_bib29","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1006\/abio.1996.9911","article-title":"A Continuous Spectrophotometeric Assay for Monoamine Oxidase and Related Enzymes in Tissue Homogenates","volume":"244","author":"Holt","year":"1997","journal-title":"Anal Biochem"},{"key":"10.1016\/j.jbc.2026.113649_bib30","doi-asserted-by":"crossref","first-page":"36","DOI":"10.4014\/jmb.1311.11088","article-title":"Peroxidase and Photoprotective Activities of Magnesium Protoporphyrin IX","volume":"24","author":"Kim","year":"2014","journal-title":"J Microbiol Biotechnol"},{"key":"10.1016\/j.jbc.2026.113649_bib31","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray Diffraction Data Collected in Oscillation Mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.jbc.2026.113649_bib32","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser Crystallographic","volume":"40","author":"McCoy","year":"2007","journal-title":"Software J Appl Cryst"},{"key":"10.1016\/j.jbc.2026.113649_bib33","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a Comprehensive Python-Based System for Macromolecular Structure Solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst D Biol Crystallogr"},{"key":"10.1016\/j.jbc.2026.113649_bib34","first-page":"539","article-title":"Fast, Scalable Generation of High-Quality Protein Multiple Sequence Alignments Using Clustal","volume":"7","author":"Sievers","year":"2011","journal-title":"Omega Mol Syst Biol"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925826025214?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925826025214?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:23:04Z","timestamp":1792092184000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925826025214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":34,"alternative-id":["S0021925826025214"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2026.113649","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural and Kinetic Basis for Selective Inhibition of Canine Myeloperoxidase by Staphylococcal Peroxidase Inhibitor (SPIN) Orthologs","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2026.113649","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"113649"}}