{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:41:58Z","timestamp":1792788118244,"version":"4.5.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:00:00Z","timestamp":1792195200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.jbc.2026.113670","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:20:44Z","timestamp":1792682444000},"page":"113670","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Site-specific m1A Installation by the MTR1 Ribozyme and Modulation of Mitochondrial mRNA Translation in vitro"],"prefix":"10.1016","author":[{"given":"Yuting","family":"Xie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoya","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1606-9259","authenticated-orcid":false,"given":"Jie","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4896-7557","authenticated-orcid":false,"given":"Yingying","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3081-1720","authenticated-orcid":false,"given":"Bo","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6301-881X","authenticated-orcid":false,"given":"Yuanlin","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2121-365X","authenticated-orcid":false,"given":"Lin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbc.2026.113670_bib1","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/j.cell.2017.05.045","article-title":"Dynamic RNA modifications in gene expression regulation","volume":"169","author":"Roundtree","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2026.113670_bib2","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1126\/science.aau1646","article-title":"RNA modifications modulate gene expression during development","volume":"361","author":"Frye","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.jbc.2026.113670_bib3","doi-asserted-by":"crossref","first-page":"D231","DOI":"10.1093\/nar\/gkab1083","article-title":"MODOMICS: a database of RNA modification pathways. 2021 update","volume":"50","author":"Boccaletto","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jbc.2026.113670_bib4","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1038\/s41392-022-01175-9","article-title":"RNA modifications: importance in immune cell biology and related diseases","volume":"7","author":"Cui","year":"2022","journal-title":"Signal Transduct Target Ther"},{"key":"10.1016\/j.jbc.2026.113670_bib5","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/s12943-024-02041-8","article-title":"The role of RNA methylation in tumor immunity and its potential in immunotherapy","volume":"23","author":"Li","year":"2024","journal-title":"Mol Cancer"},{"key":"10.1016\/j.jbc.2026.113670_bib6","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nchembio.2040","article-title":"Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome","volume":"12","author":"Li","year":"2016","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib7","doi-asserted-by":"crossref","first-page":"20","DOI":"10.3390\/biom7010020","article-title":"m1A post-transcriptional modification in tRNAs","volume":"7","author":"Oerum","year":"2017","journal-title":"Biomolecules"},{"key":"10.1016\/j.jbc.2026.113670_bib8","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nature24456","article-title":"The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution","volume":"551","author":"Safra","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113670_bib9","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/nature16998","article-title":"The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA","volume":"530","author":"Dominissini","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113670_bib10","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.molcel.2017.10.019","article-title":"Base-resolution mapping reveals distinct m1A methylome in nuclear- and mitochondrial-encoded transcripts","volume":"68","author":"Li","year":"2017","journal-title":"Mol Cell"},{"key":"10.1016\/j.jbc.2026.113670_bib11","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1002\/iub.2534","article-title":"N1-methyladenosine profiling of long non-coding RNA in colorectal cancer","volume":"73","author":"Shi","year":"2021","journal-title":"IUBMB Life"},{"key":"10.1016\/j.jbc.2026.113670_bib12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/s43587-023-00556-1","article-title":"Age-related noncanonical TRMT6-TRMT61A signaling impairs hematopoietic stem cells","volume":"4","author":"He","year":"2024","journal-title":"Nat Aging"},{"key":"10.1016\/j.jbc.2026.113670_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2025.168989","article-title":"Advances in human pre-tRNA maturation: TRMT10C and ELAC2 in focus","volume":"437","author":"Sikarwar","year":"2025","journal-title":"J Mol Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib14","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s41418-022-01044-6","article-title":"Mitochondrial RNA methyltransferase TRMT61B is a new, potential biomarker and therapeutic target for highly aneuploid cancers","volume":"30","author":"Mart\u00edn","year":"2023","journal-title":"Cell Death Differ"},{"key":"10.1016\/j.jbc.2026.113670_bib15","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/s41589-022-00976-x","article-title":"Structure and mechanism of the methyltransferase ribozyme MTR1","volume":"18","author":"Scheitl","year":"2022","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib16","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/s41586-020-2854-z","article-title":"Site-specific RNA methylation by a methyltransferase ribozyme","volume":"587","author":"Scheitl","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113670_bib17","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/s41589-022-00982-z","article-title":"Structure and mechanism of a methyltransferase ribozyme","volume":"18","author":"Deng","year":"2022","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib18","doi-asserted-by":"crossref","first-page":"4508","DOI":"10.1093\/nar\/gkad260","article-title":"Catalytic mechanism and pH dependence of a methyltransferase ribozyme (MTR1) from computational enzymology","volume":"51","author":"McCarthy","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jbc.2026.113670_bib19","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.tibs.2025.01.011","article-title":"RNA catalysis moving towards metabolic reactions: progress with ribozyme catalyzed alkyl transfer","volume":"50","author":"Lilley","year":"2025","journal-title":"Trends Biochem Sci"},{"key":"10.1016\/j.jbc.2026.113670_bib20","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0378-1119(89)90359-4","article-title":"Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension","volume":"77","author":"Horton","year":"1989","journal-title":"Gene"},{"key":"10.1016\/j.jbc.2026.113670_bib21","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1038\/nprot.2006.478","article-title":"Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts","volume":"2","author":"Frezza","year":"2007","journal-title":"Nat Protoc"},{"key":"10.1016\/j.jbc.2026.113670_bib22","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nature12730","article-title":"N6-methyladenosine-dependent regulation of messenger RNA stability","volume":"505","author":"Wang","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113670_bib23","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.molcel.2016.01.012","article-title":"Nuclear m(6)A reader YTHDC1 regulates mRNA splicing","volume":"61","author":"Xiao","year":"2016","journal-title":"Mol Cell"},{"key":"10.1016\/j.jbc.2026.113670_bib24","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1038\/nmeth.3898","article-title":"m6A-LAIC-seq reveals the census and complexity of the m6A epitranscriptome","volume":"13","author":"Molinie","year":"2016","journal-title":"Nat Methods"},{"key":"10.1016\/j.jbc.2026.113670_bib25","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.cell.2015.10.012","article-title":"5\u2032 UTR m6A promotes cap-independent translation","volume":"163","author":"Meyer","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2026.113670_bib26","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s12943-020-01172-y","article-title":"The emerging roles of N6-methyladenosine (m6A) deregulation in liver carcinogenesis","volume":"19","author":"Chen","year":"2020","journal-title":"Mol Cancer"},{"key":"10.1016\/j.jbc.2026.113670_bib27","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/s41556-018-0045-z","article-title":"Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation","volume":"20","author":"Huang","year":"2018","journal-title":"Nat Cell Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib28","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/nchembio.1432","article-title":"A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation","volume":"10","author":"Liu","year":"2014","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib29","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202500257","article-title":"Ribozyme-catalyzed site-specific labeling of RNA using O6-alkylguanine SNAP-tag substrates","volume":"64","author":"Walunj","year":"2025","journal-title":"Angew Chem Int Ed Engl"},{"key":"10.1016\/j.jbc.2026.113670_bib30","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nmeth.1321","article-title":"Imaging intracellular RNA distribution and dynamics in living cells","volume":"6","author":"Tyagi","year":"2009","journal-title":"Nat Methods"},{"key":"10.1016\/j.jbc.2026.113670_bib31","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.cell.2016.02.054","article-title":"Programmable RNA tracking in live cells with CRISPR\/Cas9","volume":"165","author":"Nelles","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.jbc.2026.113670_bib32","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202305463","article-title":"Ribozyme-catalyzed late-stage functionalization and fluorogenic labeling of RNA","volume":"62","author":"Scheitl","year":"2023","journal-title":"Angew Chem Int Ed Engl"},{"key":"10.1016\/j.jbc.2026.113670_bib33","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1126\/science.1207339","article-title":"RNA mimics of green fluorescent protein","volume":"333","author":"Paige","year":"2011","journal-title":"Science"},{"key":"10.1016\/j.jbc.2026.113670_bib34","doi-asserted-by":"crossref","first-page":"2412","DOI":"10.1021\/cb500499x","article-title":"RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking","volume":"9","author":"Dolgosheina","year":"2014","journal-title":"ACS Chem Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib35","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3390\/cells9010017","article-title":"Mitochondrial gene expression and beyond-novel aspects of cellular physiology","volume":"9","author":"Kotrys","year":"2019","journal-title":"Cells"},{"key":"10.1016\/j.jbc.2026.113670_bib36","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/s41586-022-04898-5","article-title":"Mitochondrial RNA modifications shape metabolic plasticity in metastasis","volume":"607","author":"Delaunay","year":"2022","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113670_bib37","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nature24456","article-title":"The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution","volume":"551","author":"Safra","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113670_bib38","doi-asserted-by":"crossref","first-page":"910","DOI":"10.3390\/genes13050910","article-title":"m1A RNA modification in gene expression regulation","volume":"13","author":"Jin","year":"2022","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.jbc.2026.113670_bib39","doi-asserted-by":"crossref","first-page":"19546","DOI":"10.1021\/jacs.9b10531","article-title":"Direct in vitro selection of trans-acting ribozymes for posttranscriptional, site-specific, and covalent fluorescent labeling of RNA","volume":"141","author":"Ghaem Maghami","year":"2019","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.jbc.2026.113670_bib40","doi-asserted-by":"crossref","first-page":"2826","DOI":"10.1002\/cbic.202100161","article-title":"Strategies for covalent labeling of long RNAs","volume":"22","author":"Depmeier","year":"2021","journal-title":"ChemBioChem"},{"key":"10.1016\/j.jbc.2026.113670_bib41","article-title":"Sequence-specific installation of aryl groups in RNA via DNA-catalyst conjugates","author":"Pratihar","year":"2025","journal-title":"Angew Chem Int Ed Engl"},{"key":"10.1016\/j.jbc.2026.113670_bib42","article-title":"Site-selective RNA modification by programmable DNA-small molecule conjugates","author":"Kha","year":"2025","journal-title":"Angew Chem Int Ed Engl"},{"key":"10.1016\/j.jbc.2026.113670_bib43","doi-asserted-by":"crossref","first-page":"13124","DOI":"10.1021\/ja0533702","article-title":"General deoxyribozyme-catalyzed synthesis of native 3'-5' RNA linkages","volume":"127","author":"Purtha","year":"2005","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.jbc.2026.113670_bib44","doi-asserted-by":"crossref","first-page":"3650","DOI":"10.3390\/molecules25163650","article-title":"New deoxyribozymes for the native ligation of RNA","volume":"25","author":"Scheitl","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.jbc.2026.113670_bib45","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/s41557-024-01707-6","article-title":"Engineering a DNA polymerase for modifying large RNA at specific positions","volume":"17","author":"Chen","year":"2025","journal-title":"Nat Chem"},{"key":"10.1016\/j.jbc.2026.113670_bib46","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/nature14352","article-title":"Synthesis and applications of RNAs with position-selective labelling and mosaic composition","volume":"522","author":"Liu","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113670_bib47","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1021\/acscentsci.2c01237","article-title":"Sulfonylation of RNA 2'-OH groups","volume":"9","author":"Chatterjee","year":"2023","journal-title":"ACS Cent Sci"},{"key":"10.1016\/j.jbc.2026.113670_bib48","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/978-1-62703-113-4_19","article-title":"Splint ligation of RNA with T4 DNA ligase","volume":"941","author":"Kershaw","year":"2012","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib49","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nprot.2007.530","article-title":"Direct detection of small RNAs using splinted ligation","volume":"3","author":"Maroney","year":"2008","journal-title":"Nat Protoc"},{"key":"10.1016\/j.jbc.2026.113670_bib50","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nature16471","article-title":"Crystal structure of a DNA catalyst","volume":"529","author":"Ponce-Salvatierra","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.jbc.2026.113670_bib51","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1080\/15476286.2020.1749797","article-title":"Optimization and characterization of position-selective labelling of RNA (PLOR) for diverse RNA and DNA sequences","volume":"17","author":"Zhang","year":"2020","journal-title":"RNA Biol"},{"key":"10.1016\/j.jbc.2026.113670_bib52","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkag211","article-title":"Site-specific ribozyme-mediated alkylation of DNA substrates","volume":"54","author":"He","year":"2026","journal-title":"Nucleic Acids Res"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925826025421?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925826025421?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:19Z","timestamp":1792786159000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925826025421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":52,"alternative-id":["S0021925826025421"],"URL":"https:\/\/doi.org\/10.1016\/j.jbc.2026.113670","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Site-specific m1A Installation by the MTR1 Ribozyme and Modulation of Mitochondrial mRNA Translation in vitro","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbc.2026.113670","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"113670"}}