{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:15:08Z","timestamp":1789996508763,"version":"4.0.1"},"reference-count":48,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24380050"],"award-info":[{"award-number":["JP24380050"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP15H04480"],"award-info":[{"award-number":["JP15H04480"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP16F16101"],"award-info":[{"award-number":["JP16F16101"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Bioscience and Bioengineering"],"published-print":{"date-parts":[[2018,5]]},"DOI":"10.1016\/j.jbiosc.2017.12.019","type":"journal-article","created":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T18:21:14Z","timestamp":1516213274000},"page":"525-531","source":"Crossref","is-referenced-by-count":18,"title":["Metabolic dependent and independent pH-drop shuts down VirSR quorum sensing in Clostridium perfringens"],"prefix":"10.1016","volume":"125","author":[{"given":"Keika","family":"Adachi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Ohtani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8507-6773","authenticated-orcid":false,"given":"Michio","family":"Kawano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindra Pal","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basit","family":"Yousuf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Sonomoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiro","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiosc.2017.12.019_bib1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1365-2958.1995.tb02234.x","article-title":"Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringens-mediated gas gangrene","volume":"15","author":"Awad","year":"1995","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib2","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/MMBR.00062-12","article-title":"Toxin plasmids of Clostridium perfringens","volume":"77","author":"Li","year":"2013","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib3","doi-asserted-by":"crossref","first-page":"96","DOI":"10.4161\/gmic.26419","article-title":"Host cell-induced signaling causes Clostridium perfringens to upregulate production of toxins important for intestinal infections","volume":"5","author":"Chen","year":"2014","journal-title":"Gut Microb."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib4","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1128\/JB.01135-07","article-title":"Conservation and evolutionary dynamics of the agr cell-to-cell communication system across firmicutes","volume":"190","author":"Wuster","year":"2007","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib5","doi-asserted-by":"crossref","first-page":"a012427","DOI":"10.1101\/cshperspect.a012427","article-title":"Bacterial quorum sensing: its role in virulence and possibilities for its control","volume":"2","author":"Rutherford","year":"2012","journal-title":"Cold Spring Harb Perspect Med."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib6","doi-asserted-by":"crossref","first-page":"5130","DOI":"10.3390\/s130405130","article-title":"Targeting agr- and agr-like quorum sensing systems for development of common therapeutics to treat multiple gram-positive bacterial infections","volume":"13","author":"Gray","year":"2013","journal-title":"Sensors"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib7","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1128\/JB.02614-14","article-title":"Structure-function analysis of peptide signaling in the Clostridium perfringens Agr-Like quorum sensing system","volume":"197","author":"Ma","year":"2015","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib8","doi-asserted-by":"crossref","first-page":"3919","DOI":"10.1128\/JB.01455-08","article-title":"Virulence gene regulation by the agr system in Clostridium perfringens","volume":"191","author":"Ohtani","year":"2009","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib9","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1128\/jb.178.9.2514-2520.1996","article-title":"The virR\/virS locus regulates the transcription of genes encoding extracellular toxin production in Clostridium perfringens","volume":"178","author":"Ba-Thein","year":"1996","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib10","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1046\/j.1365-2958.2000.01760.x","article-title":"Identification of novel VirR\/VirS-regulated genes in Clostridium perfringens","volume":"35","author":"Banu","year":"2000","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib11","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/S0966-842X(03)00118-5","article-title":"RNA-mediated control of virulence gene expression in bacterial pathogens","volume":"11","author":"Johansson","year":"2003","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib12","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1111\/j.1348-0421.2000.tb02528.x","article-title":"Genetic analysis of the ycgJ-metB-cysK-ygaG operon negatively regulated by the VirR\/VirS system in Clostridium perfringens","volume":"44","author":"Ohtani","year":"2000","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib13","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1128\/jb.176.6.1616-1623.1994","article-title":"The virR gene, a member of a class of two-component response regulators, regulates the production of perfringolysin O, collagenase, and hemagglutinin in Clostridium perfringens","volume":"176","author":"Shimizu","year":"1994","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib14","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1073\/pnas.022493799","article-title":"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater","volume":"99","author":"Shimizu","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib15","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1046\/j.1365-2958.2002.02743.x","article-title":"Clostridial VirR\/VirS regulon involves a regulatory RNA molecule for expression of toxins","volume":"43","author":"Shimizu","year":"2002","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib16","doi-asserted-by":"crossref","first-page":"7719","DOI":"10.1128\/JB.01573-07","article-title":"Characterization of genes regulated directly by the VirR\/VirS system in Clostridium perfringens","volume":"190","author":"Okumura","year":"2008","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib17","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1128\/JB.182.1.57-66.2000","article-title":"The VirR response regulator from Clostridium perfringens binds independently to two imperfect direct repeats located upstream of the pfoA promoter","volume":"182","author":"Cheung","year":"2000","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib18","doi-asserted-by":"crossref","first-page":"2937","DOI":"10.1128\/JB.00148-13","article-title":"Structural requirement in Clostridium perfringens collagenase mRNA 5\u2032 leader sequence for translational induction through small RNA-mRNA base pairing","volume":"195","author":"Obana","year":"2013","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib19","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1111\/j.1365-2958.2010.07258.x","article-title":"Stabilization of Clostridium perfringens collagenase mRNA by VR-RNA-dependent cleavage in 5\u2032 leader sequence","volume":"77","author":"Obana","year":"2010","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib20","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.anaerobe.2011.09.004","article-title":"Complex transcriptional regulation of citrate metabolism in Clostridium perfringens","volume":"18","author":"Yuan","year":"2012","journal-title":"Anaerobe"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib21","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.anaerobe.2009.10.003","article-title":"Identification of a two-component VirR\/VirS regulon in Clostridium perfringens","volume":"16","author":"Ohtani","year":"2010","journal-title":"Anaerobe"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib22","doi-asserted-by":"crossref","first-page":"e82514","DOI":"10.1371\/journal.pone.0082514","article-title":"Quorum sensing and self-quorum quenching in the intracellular pathogen Brucella melitensis","volume":"8","author":"Terwagne","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib23","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1462-5822.2004.00362.x","article-title":"Integration of environmental and host-derived signals with quorum sensing during plant-microbe interactions","volume":"6","author":"Newton","year":"2004","journal-title":"Cell Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib24","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.ijmm.2006.01.036","article-title":"A\u00a0network of networks: quorum sensing gene regulation in Pseudomonas aeruginosa","volume":"296","author":"Schuster","year":"2006","journal-title":"Iran. J. Med. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib25","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1128\/AEM.02014-07","article-title":"A\u00a0Rhodococcus qsdA-encoded enzyme defines a novel class of large-spectrum quorum-quenching lactonases","volume":"74","author":"Uroz","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib26","doi-asserted-by":"crossref","first-page":"5635","DOI":"10.1128\/IAI.70.10.5635-5646.2002","article-title":"N-Acylhomoserine lactones undergo lactonolysis in a pH-, temperature-, and acyl chain length-dependent manner during growth of Yersinia pseudotuberculosis and Pseudomonas aeruginosa","volume":"70","author":"Yates","year":"2002","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib27","doi-asserted-by":"crossref","first-page":"4638","DOI":"10.1073\/pnas.022056699","article-title":"Genetic control of quorum-sensing signal turnover in Agrobacterium tumefaciens","volume":"99","author":"Zhang","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib28","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1128\/MMBR.00002-06","article-title":"Messing with bacterial quorum sensing","volume":"70","author":"Gonz\u00e1lez","year":"2006","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib29","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1046\/j.1365-2958.2002.02863.x","article-title":"The luxS gene is involved in cell-cell signaling for toxin production in Clostridium perfringens","volume":"44","author":"Ohtani","year":"2002","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib30","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1111\/j.1348-0421.1992.tb02050.x","article-title":"An activity which restores theta toxin activity in some theta toxin-deficient mutants of Clostridium perfringens","volume":"36","author":"Imagawa","year":"1992","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib31","doi-asserted-by":"crossref","first-page":"fnv188","DOI":"10.1093\/femsle\/fnv188","article-title":"Rationale design of quorum-quenching peptides that target the VirSR system of Clostridium perfringens","volume":"362","author":"Singh","year":"2015","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib32","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S1389-1723(04)00279-8","article-title":"High butanol production by Clostridium saccharoperbutylacetonicum N1-4 in fed-batch culture with pH-Stat continuous butyric acid and glucose feeding method","volume":"98","author":"Tashiro","year":"2004","journal-title":"J.\u00a0Biosci. Bioeng."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib33","doi-asserted-by":"crossref","first-page":"12556","DOI":"10.1074\/jbc.R116.722710","article-title":"Impact of environmental cues on staphylococcal quorum sensing and biofilm development","volume":"291","author":"Kavanaugh","year":"2016","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib34","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1128\/IAI.60.8.3381-3388.1992","article-title":"Glucose and nonmaintained pH decrease expression of the accessory gene regulator (agr) in Staphylococcus aureus","volume":"60","author":"Regassa","year":"1992","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib35","doi-asserted-by":"crossref","first-page":"3422","DOI":"10.1128\/AEM.02763-10","article-title":"Integrated regulation of acetoin fermentation by quorum sensing and pH in Serratia plymuthica RVH1","volume":"77","author":"Moons","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib36","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1007\/s00438-003-0883-6","article-title":"Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs","volume":"269","author":"Hambraeus","year":"2003","journal-title":"Mol. Genet. Genom."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib37","doi-asserted-by":"crossref","first-page":"6379","DOI":"10.1128\/JB.00609-06","article-title":"Characterization of the Staphylococcus aureus heat shock, cold shock, stringent, and SOS responses and their effects on log-phase mRNA turnover","volume":"188","author":"Anderson","year":"2006","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib38","doi-asserted-by":"crossref","first-page":"9697","DOI":"10.1073\/pnas.112318199","article-title":"Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays","volume":"99","author":"Bernstein","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib39","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/nar\/gks1019","article-title":"Global analysis of mRNA stability in Mycobacterium tuberculosis","volume":"41","author":"Rustad","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib40","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1099\/mic.0.066266-0","article-title":"Regulation of pqs quorum sensing via catabolite repression control in Pseudomonas aeruginosa","volume":"159","author":"Zhang","year":"2013","journal-title":"Microbiology"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib41","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1186\/s12864-016-2706-2","article-title":"RNA-seq analysis of virR and revR mutants of Clostridium perfringens","volume":"17","author":"Low","year":"2016","journal-title":"BMC Genom."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib42","doi-asserted-by":"crossref","first-page":"E207","DOI":"10.3390\/toxins8070207","article-title":"Regulation of toxin production in Clostridium perfringens","volume":"8","author":"Ohtani","year":"2016","journal-title":"Toxins"},{"key":"10.1016\/j.jbiosc.2017.12.019_bib43","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00703-16","article-title":"The role of ribonuclease Y in Clostridium perfringens mRNA and processing","volume":"199","author":"Obana","year":"2017","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib44","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00310-17","article-title":"Structural and functional analysis of the Escherichia coli acid-sensing histidine kinase EvgS","volume":"199","author":"Sen","year":"2017","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib45","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.1007\/s00253-017-8137-4","article-title":"Mathematical modelling of clostridial acetone-butanol-ethanol fermentation","volume":"101","author":"Millat","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"suppl. 2","key":"10.1016\/j.jbiosc.2017.12.019_bib46","doi-asserted-by":"crossref","first-page":"S48","DOI":"10.1093\/cid\/civ523","article-title":"Evolving resistance among Gram-positive pathogens","volume":"61","author":"Munita","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib47","first-page":"e20863","article-title":"Antibiotic sensitivity of Clostridium perfringens isolated from feces in Tabriz, Iran","volume":"8","author":"Akhi","year":"2015","journal-title":"Jundishapur J. Microbiol."},{"key":"10.1016\/j.jbiosc.2017.12.019_bib48","first-page":"1","article-title":"Antimicrobial resistance: tackling a crisis for the health and wealth of nations","volume":"11","author":"O'Neill","year":"2014","journal-title":"Rev. Antimicrob. Resist."}],"container-title":["Journal of Bioscience and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1389172317309039?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1389172317309039?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,25]],"date-time":"2020-10-25T19:28:08Z","timestamp":1603654088000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1389172317309039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["S1389172317309039"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiosc.2017.12.019","relation":{},"ISSN":["1389-1723"],"issn-type":[{"value":"1389-1723","type":"print"}],"subject":[],"published":{"date-parts":[[2018,5]]}}}