{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:48:13Z","timestamp":1780393693079,"version":"3.54.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T00:00:00Z","timestamp":1654300800000},"content-version":"am","delay-in-days":368,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K18971"],"award-info":[{"award-number":["18K18971"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K21107"],"award-info":[{"award-number":["19K21107"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20H02535"],"award-info":[{"award-number":["20H02535"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009023","name":"Precursory Research for Embryonic Science and Technology","doi-asserted-by":"publisher","award":["JPMJPR19H2"],"award-info":[{"award-number":["JPMJPR19H2"]}],"id":[{"id":"10.13039\/501100009023","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018456","name":"Kanagawa Institute of Industrial Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018456","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Bioscience and Bioengineering"],"published-print":{"date-parts":[[2021,6]]},"DOI":"10.1016\/j.jbiosc.2021.02.001","type":"journal-article","created":{"date-parts":[[2021,3,7]],"date-time":"2021-03-07T08:28:35Z","timestamp":1615105715000},"page":"679-685","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["Impact of adipose-derived stem cells on engineering hair follicle germ-like tissue grafts for hair regenerative medicine"],"prefix":"10.1016","volume":"131","author":[{"given":"Rikuma","family":"Nakajima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Tate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6248-3755","authenticated-orcid":false,"given":"Tatsuto","family":"Kageyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7194-8099","authenticated-orcid":false,"given":"Junji","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiosc.2021.02.001_bib1","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1517\/14712598.2013.739601","article-title":"Therapeutic strategy for hair regeneration: hair cycle activation, niche environment modulation, wound-induced follicle neogenesis, and stem cell engineering","volume":"13","author":"Chueh","year":"2013","journal-title":"Expert Opin. Biol. Ther."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib2","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1517\/17460441.2015.1009892","article-title":"Drug discovery for alopecia: gone today, hair tomorrow","volume":"10","author":"Santos","year":"2015","journal-title":"Expert Opin. Drug Discov."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib3","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/j.1749-6632.1960.tb40920.x","article-title":"Autografts IN alopecias and other selected dermatological conditions","volume":"83","author":"Orentreich","year":"1959","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib4","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.nano.2018.08.012","article-title":"Engineering the niche for hair regeneration - a critical review","volume":"15","author":"Nilforoushzadeh","year":"2019","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib5","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1002\/sctm.19-0301","article-title":"Tissue engineering strategies for human hair follicle regeneration: how far from a hairy goal? Concise review","volume":"9","author":"Castro","year":"2020","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib6","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1046\/j.0022-202x.2001.01670.x","article-title":"Molecular mechanisms regulating hair follicle development","volume":"118","author":"Millar","year":"2002","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib7","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1111\/j.0022-202X.2005.23716.x","article-title":"Organogenesis from dissociated cells: generation of mature cycling hair follicles from skin-derived cells","volume":"124","author":"Zheng","year":"2005","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib8","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1038\/ncomms1784","article-title":"Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches","volume":"3","author":"Toyoshima","year":"2012","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib9","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.biomaterials.2012.09.083","article-title":"Scalable production of controllable dermal papilla spheroids on PVA surfaces and the effects of spheroid size on hair follicle regeneration","volume":"34","author":"Huang","year":"2013","journal-title":"Biomaterials"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib10","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.biomaterials.2017.10.056","article-title":"Spontaneous hair follicle germ (HFG) formation in\u00a0vitro, enabling the large-scale production of HFGs for regenerative medicine","volume":"154","author":"Kageyama","year":"2018","journal-title":"Biomaterials"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib11","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1089\/ten.tea.2013.0759","article-title":"Full-thickness skin with mature hair follicles generated from tissue culture expanded human cells","volume":"20","author":"Wu","year":"2014","journal-title":"Tissue Eng."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib12","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1038\/sj.jid.5700376","article-title":"Epithelial stem cells: a folliculocentric view","volume":"126","author":"Cotsarelis","year":"2006","journal-title":"J.\u00a0Invest. Dermatol."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib13","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1242\/jcs.082446","article-title":"Hair follicle dermal papilla cells at a glance","volume":"124","author":"Driskell","year":"2011","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib14","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.45977","article-title":"Skin vasculature and hair follicle cross-talking associated with stem cell activation and tissue homeostasis","volume":"8","author":"Li","year":"2019","journal-title":"Elife"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib15","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.cell.2011.08.020","article-title":"Niche crosstalk: intercellular signals at the hair follicle","volume":"146","author":"Jahoda","year":"2011","journal-title":"Cell"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib16","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/j.cell.2011.07.019","article-title":"Adipocyte lineage cells contribute to the skin stem cell niche to drive hair cycling","volume":"146","author":"Festa","year":"2011","journal-title":"Cell"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib17","doi-asserted-by":"crossref","first-page":"26436","DOI":"10.1038\/srep26436","article-title":"Assembling composite dermal papilla spheres with adipose-derived stem cells to enhance hair follicle induction","volume":"6","author":"Huang","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib18","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/nprot.2008.50","article-title":"Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in\u00a0vitro analysis and for grafting to immunodeficient mice","volume":"3","author":"Lichti","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib19","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.cell.2004.08.012","article-title":"Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche","volume":"118","author":"Blanpain","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib20","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1038\/nprot.2010.39","article-title":"Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis","volume":"5","author":"Jensen","year":"2010","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib21","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.biomaterials.2019.05.003","article-title":"Preparation of hair beads and hair follicle germs for regenerative medicine","volume":"212","author":"Kageyama","year":"2019","journal-title":"Biomaterials"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib22","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1016\/j.jbiosc.2020.08.005","article-title":"Effects of platelet-rich plasma on in\u00a0vitro hair follicle germ preparation for hair regenerative medicine","volume":"130","author":"Kageyama","year":"2020","journal-title":"J.\u00a0Biosci. Bioeng."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib23","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1038\/s41598-020-79722-z","article-title":"Hair follicle germs containing vascular endothelial cells for hair regenerative medicine","volume":"11","author":"Kageyama","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib24","doi-asserted-by":"crossref","first-page":"19385","DOI":"10.3390\/ijms141019385","article-title":"Keratin K15 as a biomarker of epidermal stem cells","volume":"14","author":"Bose","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib25","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1073\/pnas.91.1.206","article-title":"Experimental specification OF cell sorting, tissue spreading, and specific spatial patterning by quantitative differences in cadherin expression","volume":"91","author":"Steinberg","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib26","doi-asserted-by":"crossref","first-page":"20160877","DOI":"10.1098\/rsif.2016.0877","article-title":"Advances in multicellular spheroids formation","volume":"14","author":"Cui","year":"2017","journal-title":"J.\u00a0R. Soc. Interface"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib27","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.ydbio.2004.11.012","article-title":"The differential adhesion hypothesis: a direct evaluation","volume":"278","author":"Foty","year":"2005","journal-title":"Dev. Biol."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib28","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/j.bbrc.2013.07.081","article-title":"N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation","volume":"438","author":"Ishimine","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib29","doi-asserted-by":"crossref","first-page":"7336","DOI":"10.1073\/pnas.96.13.7336","article-title":"Selective activation of the versican promoter by epithelial-mesenchymal interactions during hair follicle development","volume":"96","author":"Kishimoto","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib30","doi-asserted-by":"crossref","first-page":"42777","DOI":"10.1038\/srep42777","article-title":"Induction of hair follicle dermal papilla cell properties in human induced pluripotent stem cell-derived multipotent LNGFR(+) THY-1(+) mesenchymal cells","volume":"7","author":"Veraitch","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib31","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1111\/exd.12001","article-title":"Unravelling hair follicle-adipocyte communication","volume":"21","author":"Schmidt","year":"2012","journal-title":"Exp. Dermatol."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib32","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1292\/jvms.16-0122","article-title":"Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor","volume":"78","author":"Lee","year":"2016","journal-title":"J.\u00a0Vet. Med. Sci."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib33","doi-asserted-by":"crossref","first-page":"8430","DOI":"10.1016\/j.biomaterials.2012.08.040","article-title":"An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids","volume":"33","author":"Anada","year":"2012","journal-title":"Biomaterials"},{"key":"10.1016\/j.jbiosc.2021.02.001_bib34","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1038\/s41598-018-38011-6","article-title":"Synergic effects of oxygen supply and antioxidants on pancreatic beta-cell spheroids","volume":"9","author":"Myasnikova","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbiosc.2021.02.001_bib35","doi-asserted-by":"crossref","first-page":"531","DOI":"10.2174\/1574888X12666170522114307","article-title":"Hair regeneration therapy: application of adipose-derived stem cells","volume":"12","author":"Fukuoka","year":"2017","journal-title":"Curr. Stem Cell Res. Ther."}],"container-title":["Journal of Bioscience and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1389172321000293?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1389172321000293?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T14:00:12Z","timestamp":1761400812000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1389172321000293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["S1389172321000293"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiosc.2021.02.001","relation":{},"ISSN":["1389-1723"],"issn-type":[{"value":"1389-1723","type":"print"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Impact of adipose-derived stem cells on engineering hair follicle germ-like tissue grafts for hair regenerative medicine","name":"articletitle","label":"Article Title"},{"value":"Journal of Bioscience and Bioengineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiosc.2021.02.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Society for Biotechnology, Japan. All rights reserved.","name":"copyright","label":"Copyright"}]}}