{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T14:07:48Z","timestamp":1761401268627,"version":"build-2065373602"},"reference-count":34,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T00:00:00Z","timestamp":1686700800000},"content-version":"am","delay-in-days":348,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Bioscience and Bioengineering"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.jbiosc.2022.03.006","type":"journal-article","created":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T07:43:44Z","timestamp":1650267824000},"page":"70-76","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Hapten-labeled fusion-polymerase chain reaction of multiple marker genes for the application of immunochromatographic test"],"prefix":"10.1016","volume":"134","author":[{"given":"Atsushi","family":"Tabata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rina","family":"Shirai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruka","family":"Miki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukihiro","family":"Nishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Kashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomi","family":"Aoyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3161-8236","authenticated-orcid":false,"given":"Shu","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Momoyo","family":"Azuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshifumi","family":"Tomoyasu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9995-7635","authenticated-orcid":false,"given":"Hideaki","family":"Nagamune","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiosc.2022.03.006_bib1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1101\/SQB.1986.051.01.032","article-title":"Specific enzymatic amplification of DNA in\u00a0vitro: the polymerase chain reaction","volume":"51","author":"Mullis","year":"1986","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib2","doi-asserted-by":"crossref","first-page":"S65","DOI":"10.1101\/gr.3.4.S65","article-title":"Multiplex PCR: advantages, development, and applications","volume":"3","author":"Edwards","year":"1994","journal-title":"PCR Methods Appl."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib3","doi-asserted-by":"crossref","first-page":"461","DOI":"10.3855\/jidc.3856","article-title":"Identification of the most common pathogenic bacteria in patients with suspected sepsis by multiplex PCR","volume":"8","author":"Arabestani","year":"2014","journal-title":"J.\u00a0Infect. Dev. Ctries."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib4","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.ijantimicag.2018.10.022","article-title":"Development and validation of a multiplex polymerase chain reaction assay for detection of the five families of plasmid-encoded colistin resistance","volume":"53","author":"Jousset","year":"2019","journal-title":"Int. J. Antimicrob. Agents"},{"key":"10.1016\/j.jbiosc.2022.03.006_bib5","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1111\/1750-3841.15033","article-title":"A\u00a0multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens","volume":"85","author":"Tao","year":"2020","journal-title":"J.\u00a0Food Sci."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib6","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s13205-021-02759-y","article-title":"Multiplex PCR method for simultaneous detection of five pathogenic bacteria closely related to foodborne diseases","volume":"11","author":"Chen","year":"2021","journal-title":"3 Biotech."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib7","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.cll.2015.02.006","article-title":"Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis","volume":"35","author":"Zhang","year":"2015","journal-title":"Clin. Lab. Med."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib8","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/j.cmi.2017.11.018","article-title":"Multiplex PCR system for the rapid diagnosis of respiratory virus infection: systematic review and meta-analysis","volume":"24","author":"Huang","year":"2018","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib9","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1128\/CMR.13.4.559","article-title":"Multiplex PCR: optimization and application in diagnostic virology","volume":"13","author":"Elnifro","year":"2000","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib10","doi-asserted-by":"crossref","first-page":"4895","DOI":"10.1093\/nar\/17.12.4895","article-title":"Precise gene fusion by PCR","volume":"17","author":"Yon","year":"1989","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib11","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1007\/s12033-017-0050-7","article-title":"Efficient one-step fusion PCR based on dual-asymmetric primers and two-step annealing","volume":"60","author":"Liu","year":"2018","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib12","doi-asserted-by":"crossref","first-page":"100759","DOI":"10.1016\/j.mex.2019.12.001","article-title":"Optimization of overlap extension PCR for efficient transgene construction","volume":"7","author":"Hilgarth","year":"2020","journal-title":"MethodsX"},{"key":"10.1016\/j.jbiosc.2022.03.006_bib13","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/978-1-61779-228-1_27","article-title":"Overlap extension PCR: an efficient method for transgene construction","volume":"772","author":"Nelson","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib14","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1002\/jobm.201700455","article-title":"Using overlap-extension PCR technique to fusing genes for constructing recombinant plasmids","volume":"58","author":"Lu","year":"2018","journal-title":"J.\u00a0Basic Microbiol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib15","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1128\/JCM.00476-17","article-title":"Point-of-care testing for infectious diseases: past, present, and future","volume":"55","author":"Kozel","year":"2017","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib16","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1128\/JCM.02262-09","article-title":"Development of an immunochromatographic assay specifically detecting pandemic H1N1 (2009) influenza virus","volume":"48","author":"Miyoshi-Akiyama","year":"2010","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0225365","article-title":"Evaluation of a nanophosphor lateral-flow assay for self-testing for herpes simplex virus type 2 seropositivity","volume":"14","author":"Goux","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.jbiosc.2022.03.006_bib18","doi-asserted-by":"crossref","first-page":"12550","DOI":"10.1021\/acsomega.0c01554","article-title":"Rapid detection of IgM antibodies against the SARS-CoV-2 virus via colloidal gold nanoparticle-based lateral-flow assay","volume":"5","author":"Huang","year":"2020","journal-title":"ACS Omega"},{"key":"10.1016\/j.jbiosc.2022.03.006_bib19","doi-asserted-by":"crossref","first-page":"5345","DOI":"10.1039\/D0AN00629G","article-title":"Development of a lateral flow immunoassay strip for rapid detection of IgG antibody against SARS-CoV-2 virus","volume":"145","author":"Wen","year":"2020","journal-title":"Analyst"},{"key":"10.1016\/j.jbiosc.2022.03.006_bib20","doi-asserted-by":"crossref","first-page":"403","DOI":"10.3390\/diagnostics10060403","article-title":"Development of a nucleic acid lateral flow immunoassay for the detection of human polyomavirus BK","volume":"10","author":"Huang","year":"2020","journal-title":"Diagnostics (Basel)"},{"key":"10.1016\/j.jbiosc.2022.03.006_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0137791","article-title":"Rapid molecular detection of multidrug-resistant Tuberculosis by PCR-nucleic acid lateral flow immunoassay","volume":"10","author":"Kamphee","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.jbiosc.2022.03.006_bib22","doi-asserted-by":"crossref","first-page":"3520","DOI":"10.1128\/JCM.01426-12","article-title":"Direct blood PCR in combination with nucleic acid lateral flow immunoassay for detection of Plasmodium species in settings where malaria is endemic","volume":"50","author":"Mens","year":"2012","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib23","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jmoldx.2017.09.004","article-title":"Plasmodium detection and differentiation by direct-on-blood PCR nucleic acid lateral flow immunoassay: development, validation, and evaluation","volume":"20","author":"Roth","year":"2018","journal-title":"J.\u00a0Mol. Diagn."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib24","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s12917-019-1774-3","article-title":"Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2","volume":"15","author":"Zhuang","year":"2019","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib25","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.mimet.2018.06.010","article-title":"Development of a rapid PCR-Nucleic Acid Lateral Flow Immunoassay (PCR-NALFIA) based on rDNA IGS sequence analysis for the detection of Macrophomina phaseolina in soil","volume":"151","author":"Pecchia","year":"2018","journal-title":"J.\u00a0Microbiol. Methods"},{"key":"10.1016\/j.jbiosc.2022.03.006_bib26","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.ijfoodmicro.2016.12.003","article-title":"Rapid detection\u00a0of\u00a0methicillin-resistant Staphylococcus aureus in pork using a nucleic acid-based lateral flow immunoassay","volume":"243","author":"Zhang","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib27","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.vetmic.2016.08.009","article-title":"Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes mecA and mecC","volume":"200","author":"Seidel","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib28","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1128\/AAC.48.6.2244-2250.2004","article-title":"Complete sequences of six penicillin-binding protein genes from 40 Streptococcus pneumoniae clinical isolates collected in Japan","volume":"48","author":"Sanbongi","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib29","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1574-6968.2010.02044.x","article-title":"Real-time PCR assays for the detection and quantification of Streptococcus pneumoniae","volume":"310","author":"Park","year":"2010","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib30","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1099\/jmm.0.017798-0","article-title":"Identification of the cpsA gene as a specific marker for the discrimination of Streptococcus pneumoniae from viridans group streptococci","volume":"59","author":"Park","year":"2010","journal-title":"J.\u00a0Med. Microbiol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib31","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1128\/JCM.00051-08","article-title":"Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay","volume":"46","author":"Fukushima","year":"2008","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib32","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1128\/AAC.03395-14","article-title":"Detection of single-nucleotide polymorphisms in Plasmodium falciparum by PCR primer extension and lateral flow immunoassay","volume":"59","author":"Moers","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib33","doi-asserted-by":"crossref","first-page":"5783","DOI":"10.1021\/acs.jafc.0c07980","article-title":"Nucleic acid amplification techniques in immunoassay: an integrated approach with hybrid performance","volume":"69","author":"Sang","year":"2021","journal-title":"J.\u00a0Agric. Food Chem."},{"key":"10.1016\/j.jbiosc.2022.03.006_bib34","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1021\/acsfoodscitech.0c00085","article-title":"Development of a polymerase chain reaction lateral flow immunoassay for rapid authentication of venison in food products","volume":"1","author":"Zhao","year":"2021","journal-title":"ACS Food Sci. Technol."}],"container-title":["Journal of Bioscience and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1389172322000846?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1389172322000846?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T14:02:50Z","timestamp":1761400970000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1389172322000846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["S1389172322000846"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiosc.2022.03.006","relation":{},"ISSN":["1389-1723"],"issn-type":[{"type":"print","value":"1389-1723"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Hapten-labeled fusion-polymerase chain reaction of multiple marker genes for the application of immunochromatographic test","name":"articletitle","label":"Article Title"},{"value":"Journal of Bioscience and Bioengineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiosc.2022.03.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Society for Biotechnology, Japan. All rights reserved.","name":"copyright","label":"Copyright"}]}}