{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T11:50:43Z","timestamp":1768564243258,"version":"3.49.0"},"reference-count":32,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002973","name":"Daiichi-Sankyo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002973","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Bioscience and Bioengineering"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.jbiosc.2025.08.006","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T13:29:13Z","timestamp":1757338153000},"page":"378-385","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Optimization of heterologous protein production process using genetically engineered Ogataea minuta toward industrial-scale manufacturing"],"prefix":"10.1016","volume":"140","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2820-5334","authenticated-orcid":false,"given":"Masashi","family":"Tsuda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Nakatani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Baba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Nonaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0291-8299","authenticated-orcid":false,"given":"Takehiko","family":"Yoko-o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6401-5071","authenticated-orcid":false,"given":"Yasunori","family":"Chiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiosc.2025.08.006_bib1","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1038\/s41587-022-01582-x","article-title":"Biopharmaceutical benchmarks 2022","volume":"40","author":"Walsh","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2023.108121","article-title":"Challenges and progress towards industrial recombinant protein production in yeasts: a review","volume":"64","author":"De Brabander","year":"2023","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib3","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/s12033-019-00164-8","article-title":"Yeast expression systems: overview and recent advances","volume":"61","author":"Baghban","year":"2019","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib4","first-page":"1","article-title":"Yeast synthetic biology for the production of recombinant therapeutic proteins","volume":"15","author":"Kim","year":"2015","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib5","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1038\/nbt1287-1305","article-title":"High-level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris","volume":"5","author":"Tschopp","year":"1987","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib6","doi-asserted-by":"crossref","first-page":"5301","DOI":"10.1007\/s00253-014-5732-5","article-title":"Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production","volume":"98","author":"Ahmad","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib7","first-page":"354","article-title":"Pichia pastoris: a workhorse for recombinant protein production","volume":"2","author":"Fickers","year":"2014","journal-title":"Curr. Res. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib8","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.jbiosc.2017.03.001","article-title":"Efficient antibody production in the methylotrophic yeast Ogataea minuta by overexpression of chaperones","volume":"124","author":"Suzuki","year":"2017","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib9","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.jbiosc.2022.12.006","article-title":"Selection of the methylotrophic yeast Ogataea minuta as a high-producing host for heterologous protein expression","volume":"135","author":"Tsuda","year":"2023","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib10","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s11274-024-04008-9","article-title":"Recent progress on heterologous protein production in methylotrophic yeast systems","volume":"40","author":"Tsuda","year":"2024","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib11","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1093\/bbb\/zbae160","article-title":"Development of a protein production system using Ogataea minuta alcohol oxidase-deficient strain under reduced-methanol-consumption conditions","volume":"89","author":"Tsuda","year":"2024","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib12","doi-asserted-by":"crossref","first-page":"94","DOI":"10.3389\/fbioe.2019.00094","article-title":"Advances in using Hansenula polymorpha as chassis for recombinant protein production","volume":"7","author":"Manfr\u00e3o-Netto","year":"2019","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib13","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1111\/j.1567-1364.2006.00116.x","article-title":"Production of Man5GlcNAc2-type sugar chain by the methylotrophic yeast Ogataea minuta","volume":"6","author":"Kuroda","year":"2006","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib14","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1128\/AEM.02106-07","article-title":"Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta","volume":"74","author":"Kuroda","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib15","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s40259-018-0278-1","article-title":"Anticalin\u00ae proteins as therapeutic agents in human diseases","volume":"32","author":"Rothe","year":"2018","journal-title":"BioDrugs"},{"key":"10.1016\/j.jbiosc.2025.08.006_bib16","series-title":"Molecular cloning: a laboratory manual","author":"Sambrook","year":"2001"},{"key":"10.1016\/j.jbiosc.2025.08.006_bib17","series-title":"Novel proteins specific for calcitonin gene-related peptide","author":"Kubota","year":"2017"},{"key":"10.1016\/j.jbiosc.2025.08.006_bib18","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1186\/s12934-021-01617-z","article-title":"Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up","volume":"20","author":"Madhavan","year":"2021","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib19","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1111\/j.1567-1364.2007.00291.x","article-title":"Antibody expression in protease-deficient strains of the methylotrophic yeast Ogataea minuta","volume":"7","author":"Kuroda","year":"2007","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib20","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/978-1-59745-456-8_2","article-title":"Vectors and strains for expression","volume":"389","author":"Lin-Cereghino","year":"2007","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib21","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0141-0229(97)00042-2","article-title":"Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fedbatch fermentations","volume":"22","author":"Chiruvolu","year":"1997","journal-title":"Enzyme Microb. Technol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib22","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s12934-015-0292-7","article-title":"Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter","volume":"14","author":"Capone","year":"2015","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib23","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1002\/bit.26529","article-title":"Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors","volume":"115","author":"Vogl","year":"2018","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib24","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0168-1656(03)00003-8","article-title":"Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes","volume":"102","author":"Jahic","year":"2003","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib25","first-page":"2490","article-title":"PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors","volume":"6","author":"Ammerer","year":"1986","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib26","doi-asserted-by":"crossref","first-page":"5 684","DOI":"10.1007\/s00253-004-1803-3","article-title":"Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium","volume":"67","author":"G\u00f6rgens","year":"2005","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib27","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.fbp.2019.09.011","article-title":"Continuous fermentation of recombinant Pichia pastoris Mut+ producing HBsAg: optimizing dilution rate and determining strain-specific parameters","volume":"118","author":"Rahimi","year":"2019","journal-title":"Food Bioprod. Process."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib28","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jbiotec.2017.01.008","article-title":"A review of control strategies for manipulating the feed rate in fed-batch fermentation processes","volume":"245","author":"Mears","year":"2017","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib29","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/j.jprocont.2007.01.019","article-title":"Oxygen control for an industrial pilot-scale fed-batch filamentous fungal fermentation","volume":"17","author":"Bodizs","year":"2007","journal-title":"J. Process. Control."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib30","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s12010-007-0003-x","article-title":"Expression of recombinant proteins in Pichia pastoris","volume":"142","author":"Li","year":"2007","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib31","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1475-2859-11-22","article-title":"Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway","volume":"11","author":"Krainer","year":"2012","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.jbiosc.2025.08.006_bib32","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/fox008","article-title":"Dynamic modeling reveals a three-step response of Saccharomyces cerevisiae to high CO2 levels accompanied by increasing ATP demands","volume":"17","author":"Eigenstetter","year":"2017","journal-title":"FEMS Yeast Res."}],"container-title":["Journal of Bioscience and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1389172325002208?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1389172325002208?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T03:45:53Z","timestamp":1761623153000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1389172325002208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":32,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["S1389172325002208"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiosc.2025.08.006","relation":{},"ISSN":["1389-1723"],"issn-type":[{"value":"1389-1723","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimization of heterologous protein production process using genetically engineered Ogataea minuta toward industrial-scale manufacturing","name":"articletitle","label":"Article Title"},{"value":"Journal of Bioscience and Bioengineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiosc.2025.08.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Society for Biotechnology, Japan. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}